Abstract
Soil salinity is a major constraint affecting crop growth and productivity, and limiting sustainable agricultural development in arid zones. Understanding the molecular mechanisms underlying the adaptation of canola to salt stress is important to improve salt tolerance and promote its cultivation in saline soils. To elucidate the metabolic and transcriptional regulatory mechanisms in canola under salt stress, the seedling roots of the control (no salt treatment) and roots of canola seedlings subjected to 72 h of 200 mM NaCl stress (hydroponics) were collected for metabolomic analysis, supplemented with RNA-Seq analysis and quantitative real-time PCR (qRT-PCR) validation. Metabolomic analysis showed that compared with the control, the metabolites of lipids accumulated more under NaCl stress, including unsaturated fatty acids (linoleic acid, dihomo-gamma-linolenic acid, oleic acid, nervonic acid, alpha-linolenic acid), glycerophospholipids (1-palmitoyl-sn-glycero-3-phosphocholine, 1-oleoyl-sn-glycero-3-phosphocholine, 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine) lecithin (phosphorylcholine), sphingolipids (N-palmitoylsphingosine) and steroids and steroid derivatives (25-hydroxyvitamin D3); while the metabolism of most amino acids (such as l-valine, l-threonine, l-isoleucine, l-glutamate, l-phenylalanine) and carbohydrates (such as d-fructose, cellobiose, sucrose, d-mannose) were lower. Both transcriptomic and metabolomic pathway analysis indicated that lipid metabolism was an important metabolic pathway in canola roots under NaCl stress. In summary, canola seedling roots could respond to NaCl stress through lipid metabolism genes and metabolites, which improved our knowledge in molecular mechanisms encoding NaCl tolerance in canola.
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Data availability
All Gene ID and annotation files could be obtained from Brassica napus genome v4.1 (https://www.genoscope.cns.fr/brassicanapus/data/). Raw data and other data generated or analyzed were included in this published article in this study (Table S1, Table S2, Table S3, Table S4, Table S5, Table S6, Table S7, Table S8, Table S9, Fig. S1, Fig. S2, Fig. S3). The datasets used and/or analysed have been deposited in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA, https://www.ncbi.nlm.nih.gov/sra). The accession number is PRJNA721127 (https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA721127), which includes 21 accession items (SAMN18700206–SAMN18700226).
Abbreviations
- RNA-seq:
-
RNA sequencing
- qRT-PCR:
-
Quantitative real-time polymerase chain reaction
- LC–MS:
-
Liquid chromatography mass spectrometry
- PCA:
-
Principal components analysis
- PLS-DA:
-
Partial least squares discriminant analysis
- VIP:
-
Variable importance in projection
- Log2FC:
-
Logarithm of fold change with base 2
- DEGs:
-
Differentially expressed genes
- GO:
-
Gene ontology
- KEGG:
-
Kyoto Encyclopedia of Genes and Genomes
- GABA:
-
4-Amino butanoate
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This research was financially supported by the Science and Technology Cooperation Program of XPCC (2020BC001) and the Major Program of XPCC (2018AA005).
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WW and FZ designed the research. WW, LS performed the experiments. WW, JP and LY performed the data analysis and interpretation. WW, JP and LY prepared the figures and tables. WW, FZ, JP and KHMS wrote the manuscript. All authors read, commented on- and approved the manuscript.
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Communicated by Zsófia Bánfalvi.
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Wang, W., Pang, J., Zhang, F. et al. Transcriptomic and metabolomics-based analysis of key biological pathways reveals the role of lipid metabolism in response to salt stress in the root system of Brassica napus. Plant Growth Regul 97, 127–141 (2022). https://doi.org/10.1007/s10725-021-00788-4
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DOI: https://doi.org/10.1007/s10725-021-00788-4