Abstract
An indigo-producing strain was isolated from activated sludge and identified as Comamonas sp. based on 16S rRNA analysis. It produced indigo at 26.5 mg/l with a conversion of indole to indigo of 47%. Indole at 50 mg/l plus 200 mg naphthalene/l gave 32.2 mg indigo/l with a 58% conversion. A pathway for indigo formation is proposed. This is the first study of indigo biosynthesis by Comamonas sp.
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Acknowledgments
The authors would like to thank the National Natural Science Foundation of China (Nos. 21176040, 51078054 and 20923006), and the Open Project of State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (No. QAK201009) for their financial supports. We also thank the EssayStar Company (http:www.//essaystar.com/) for their English language editing.
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Qu, Y., Zhang, X., Ma, Q. et al. Indigo biosynthesis by Comamonas sp. MQ. Biotechnol Lett 34, 353–357 (2012). https://doi.org/10.1007/s10529-011-0778-2
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DOI: https://doi.org/10.1007/s10529-011-0778-2