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Electrochemiluminescence immunoassay using a paper electrode incorporating porous silver and modified with mesoporous silica nanoparticles functionalized with blue-luminescent carbon dots

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Abstract

We have prepared carbon dots (C-dots) by pyrolysis of citric acid. The one-pot synthetic strategy is time-saving and simple and the resulting C-dots display blue luminescence with high quantum yield at an anodic potential of +0.6 V. An electrochemiluminescence (ECL) immunoassay was designed for the sensitive determination of cancer antigen 125 (CA125) by using amino-functionalized mesoporous silica nanoparticles (NH2-MSNs) capped with C-dots. These act as amplification labels on a novel kind of paper working electrode modified with silver nanoparticles (Ag-PWE). This electrode was fabricated by a seed-mediated growth approach and served as a support for the attachment of the antibody. The NH2-MSNs possess higher volume-to-surface ratio than the solid nanoparticles that can carry more C-dots which improves the sensitivity of the immunoassay. The ECL immunoassay was operated by using triethanolamine as a coreactant that is less toxic and more soluble than other popular coreactants. The assay has a linear response over a wide range of 0.01–50 U mL−1, and the detection limit is 4.3 mU mL−1. This makes the method one of the most sensitive assays for CA125.

Electrochemiluminescence immunoassay using a paper electrode incorporating porous silver and modified with mesoporous silica nanoparticles functionalized with blue-luminescent carbon dots

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Acknowledgments

This work was financially supported by Natural Science Research Foundation of China (21277058, 21175058, 21207048), Natural Science Foundation of Shandong Province, China (ZR2012BZ002, ZR2011BQ019).

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Authors and Affiliations

  1. Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, People’s Republic of China

    Weiyan Liu, Chao Ma, Hongmei Yang, Yan Zhang, Mei Yan & Jinghua Yu

  2. Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, People’s Republic of China

    Shenguang Ge

  3. Cancer Research Center, Shandong Tumor Hospital, Jinan, 250012, People’s Republic of China

    Xianrang Song

Authors
  1. Weiyan Liu
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  2. Chao Ma
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  3. Hongmei Yang
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  4. Yan Zhang
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  5. Mei Yan
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  6. Shenguang Ge
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  7. Jinghua Yu
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  8. Xianrang Song
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Correspondence to Mei Yan.

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Liu, W., Ma, C., Yang, H. et al. Electrochemiluminescence immunoassay using a paper electrode incorporating porous silver and modified with mesoporous silica nanoparticles functionalized with blue-luminescent carbon dots. Microchim Acta 181, 1415–1422 (2014). https://doi.org/10.1007/s00604-014-1286-6

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  • DOI: https://doi.org/10.1007/s00604-014-1286-6

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