[go: up one dir, main page]
Skip to main content
Springer Nature Link
Log in

Miniaturized devices for isothermal DNA amplification addressing DNA diagnostics

  • Technical Paper
  • Published:
Microsystem Technologies Aims and scope Submit manuscript

Abstract

Microfluidics is an emerging technology enabling the development of lab-on-a-chip systems for clinical diagnostics, drug discovery and screening, food safety and environmental analysis. Currently, available nucleic acid diagnostic tests take advantage of polymerase chain reaction that allows exponential amplification of portions of nucleic acid sequences that can be used as indicators for the identification of various diseases. At the same time, isothermal methods for DNA amplification are being developed and are preferred for their simplified protocols and the elimination of thermocycling. Here, we present a low-cost and fast DNA amplification device for isothermal helicase dependent amplification implemented in the detection of mutations related to breast cancer as well as the detection of Salmonella pathogens. The device is fabricated by mass production amenable technologies on printed circuit board substrates, where copper facilitates the incorporation of on-chip microheaters, defining the thermal zone necessary for isothermal amplification methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Ahmad F, Hashsham SA (2012) Miniaturized nucleic acid amplification systems for rapid and point-of-care diagnostics: a review. Anal Chim Acta 733:1–15. doi:10.1016/j.aca.2012.04.031

    Article  Google Scholar 

  • Aracil C, Perdigones F, Moreno JM, Luque A, Quero JM (2015) Portable lab-on-PCB platform for autonomous micromixing. Microelectron Eng 131:13–18. doi:10.1016/j.mee.2014.10.018

    Article  Google Scholar 

  • Asiello PJ, Baeumner AJ (2011) Miniaturized isothermal nucleic acid amplification, a review. Lab Chip 11:1420–1430. doi:10.1039/c0lc00666a

    Article  Google Scholar 

  • Erickson D, Li D (2004) Integrated microfluidic devices. Anal Chim Acta 507:11–26. doi:10.1016/j.aca.2003.09.019

    Article  Google Scholar 

  • Fang X, Liu Y, Kong J, Jiang X (2010) Loop-mediated isothermal amplification integrated on microfluidic chips for point-of-care quantitative detection of pathogens. Anal Chem 82:3002–3006. doi:10.1021/ac1000652

    Article  Google Scholar 

  • Gill P, Ghaemi A (2008) Nucleic acid isothermal amplification technologies—a review. Nucleosides Nucleotides Nucleic Acids 27:224–243. doi:10.1080/15257770701845204

    Article  Google Scholar 

  • Haeberle S, Zengerle R (2007) Microfluidic platforms for lab-on-a-chip applications. Lab Chip 7:1094–1110. doi:10.1039/b706364b

    Article  Google Scholar 

  • Kaprou G et al (2015) Miniaturized devices towards an integrated lab-on-a-chip platform for DNA diagnostics. In: Progress in biomedical optics and imaging, Proceedings of SPIE. doi:10.1117/12.2181953

  • Kefala IN, Papadopoulos VE, Karpou G, Kokkoris G, Papadakis G, Tserepi A (2015) A labyrinth split and merge micromixer for bioanalytical applications. Microfluid Nanofluid. doi:10.1007/s10404-015-1610-4

    Google Scholar 

  • Kopp MU, De Mello AJ, Manz A (1998) Chemical amplification: continuous-flow PCR on a chip. Science 280:1046–1048. doi:10.1126/science.280.5366.1046

    Article  Google Scholar 

  • Lutz S et al (2010) Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA). Lab Chip 10:887–893. doi:10.1039/b921140c

    Article  Google Scholar 

  • Mahalanabis M, Do J, Almuayad H, Zhang JY, Klapperich CM (2010) An integrated disposable device for DNA extraction and helicase dependent amplification. Biomed Microdev 12:353–359. doi:10.1007/s10544-009-9391-8

    Article  Google Scholar 

  • Mahmoudian L, Kaji N, Tokeshi M, Nilsson M, Baba Y (2008) Rolling circle amplification and circle-to-circle amplification of a specific gene integrated with electrophoretic analysis on a single chip. Anal Chem 80:2483–2490. doi:10.1021/ac702289j

    Article  Google Scholar 

  • Mark D, Haeberle S, Roth G, Von Stetten F, Zengerle R (2010) Microfluidic lab-on-a-chip platforms: requirements, characteristics and applications. Chem Soc Rev 39:1153–1182. doi:10.1039/b820557b

    Article  Google Scholar 

  • Moschou D et al (2013) Integrated biochip for PCR-based DNA amplification and detection on capacitive biosensors. In: Progress in biomedical optics and imaging—proceedings of SPIE. doi:10.1117/12.2017690

  • Moschou D, Vourdas N, Kokkoris G, Papadakis G, Parthenios J, Chatzandroulis S, Tserepi A (2014) All-plastic, low-power, disposable, continuous-flow PCR chip with integrated microheaters for rapid DNA amplification. Sens Actuators B Chem 199:470–478. doi:10.1016/j.snb.2014.04.007

    Article  Google Scholar 

  • Papadakis G, Gizeli E (2014) Screening for mutations in BRCA1 and BRCA2 genes by measuring the acoustic ratio with QCM. Anal Methods 6:363–371. doi:10.1039/c3ay41143e

    Article  Google Scholar 

  • Papadopoulos VE et al (2014) A passive micromixer for enzymatic digestion of DNA. Microelectron Eng 124:42–46. doi:10.1016/j.mee.2014.04.011

    Article  Google Scholar 

  • Papadopoulos VE, Kokkoris G, Kefala IN, Tserepi A (2015) Comparison of continuous-flow and static-chamber μPCR devices through a computational study: the potential of flexible polymeric substrates. Microfluid Nanofluid 19:867–882. doi:10.1007/s10404-015-1613-1

    Article  Google Scholar 

  • Shen K, Chen X, Guo M, Cheng J (2005) A microchip-based PCR device using flexible printed circuit technology. Sens Actuators B Chem 105:251–258. doi:10.1016/j.snb.2004.05.069

    Article  Google Scholar 

  • Tsougeni K et al (2016) Plasma nanotextured polymeric lab-on-a-chip for highly efficient bacteria capture and lysis. Lab Chip. doi:10.1039/C5LC01217A

    Google Scholar 

  • Vincent M, Xu Y, Kong H (2004) Helicase-dependent isothermal DNA amplification. EMBO Rep 5:795–800. doi:10.1038/sj.embor.7400200

    Article  Google Scholar 

  • Vorkas PA, Christopoulos K, Kroupis C, Lianidou ES (2010) Mutation scanning of exon 20 of the BRCA1 gene by high-resolution melting curve analysis. Clin Biochem 43:178–185. doi:10.1016/j.clinbiochem.2009.08.024

    Article  Google Scholar 

  • Wego A, Richter S, Pagel L (2001) Fluidic microsystems based on printed circuit board technology. J Micromech Microeng 11:528–531. doi:10.1088/0960-1317/11/5/313

    Article  Google Scholar 

  • Wu A, Wang L, Jensen E, Mathies R, Boser B (2010) Modular integration of electronics and microfluidic systems using flexible printed circuit boards. Lab Chip 10:519–521. doi:10.1039/b922830f

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge Dr. S. Chatzandroulis, NCSR “Demokritos”, for the development of the temperature control unit, to be presented in detail in future work. This work is partly funded by the General Secretariat for Research and Technology/Ministry of Education, Greece and European Regional Development Fund (Sectoral Operational Program: Competitiveness and Entrepreneurship, NSRF 2007-2013)/European Commission (“SYNERGASIA II” project “LambSense: Converging Lamb wave sensors with microtechnologies towards an integrated Lab-on-chip for clinical diagnostics” 11SYN-5-502) and the EC under FP7-ICT-2011.3.2 “LOVE-FOOD: Love wave fully integrated Lab-on-Chip platform for food pathogen detection” (Grant Agreement No: 317742).

Author information

Author notes

  1. D. P. Papageorgiou

    Present address: Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA

Authors and Affiliations

  1. Institute of Nanoscience and Nanotechnology, NCSR “Demokritos”, Agia Paraskevi, 153 10, Athens, Greece

    G. D. Kaprou, D. P. Papageorgiou, G. Kokkoris, V. Papadopoulos, I. Kefala & A. Tserepi

  2. Department of Biology, University of Crete, Vassilika Vouton, Heraklion, Greece

    G. D. Kaprou & E. Gizeli

  3. Institute of Molecular Biology and Biotechnology, FORTH, 70013, Heraklion, Crete, Greece

    G. Papadakis & E. Gizeli

Authors
  1. G. D. Kaprou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Papadakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. P. Papageorgiou
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. G. Kokkoris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. Papadopoulos
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. I. Kefala
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. E. Gizeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Tserepi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to G. D. Kaprou.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kaprou, G.D., Papadakis, G., Papageorgiou, D.P. et al. Miniaturized devices for isothermal DNA amplification addressing DNA diagnostics. Microsyst Technol 22, 1529–1534 (2016). https://doi.org/10.1007/s00542-015-2750-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00542-015-2750-x

Keywords