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Kinetics of VO2 limb blood flow and regional muscle deoxygenation in young adults during moderate intensity, knee-extension exercise

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Abstract

The kinetics of pulmonary O2 uptake \( \left( {\dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} } \right), \) limb blood flow (LBF) and deoxygenation (ΔHHb) of the vastus lateralis (VL) and vastus medialis (VM) muscles during the transition to moderate-intensity knee-extension exercise (MOD) was examined. Seven males (27 ± 5 years; mean ± SD) performed repeated step transitions (n = 4) from passive exercise to MOD. Breath by breath \( \dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} , \) femoral artery LBF, and VL and VM muscle ∆HHb were measured, respectively, by mass spectrometer and volume turbine, Doppler ultrasound and near-infrared spectroscopy. Phase 2 \( \dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} , \) LBF, and ∆HHb data were fit with a mono-exponential model. The time constant (τ) of the \( \dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} \) and LBF response were not different (\( \tau \dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} , \) 24 ± 6 s; τLBF, 23 ± 8 s). The ∆HHb response did not differ between VL and VM in amplitude (VL 6.97 ± 4.22 a.u.; VM 7.24 ± 3.99 a.u.), time delay (∆HHbTD: VL 17 ± 2 s; VM 15 ± 1 s), time constant (τ∆HHb: VL 11 ± 6 s; VM 13 ± 4 s), or effective time constant [τ′∆HHb (= ∆HHbTD + τ∆HHb): VL 28 ± 7 s; VM 28 ± 4 s]. Adjustments in ∆HHb in VL and VM depict a similar balance of regional O2 delivery and utilization within the quadriceps muscle group. The τ′∆HHb and \( \tau \dot{V}{{{\text{O}}_{{ 2\,{\text{p}}}} }} \) were similar, however, the ∆HHb displayed an “overshoot” relative to the steady-state levels reflecting a slower alteration of microvascular blood flow (O2 delivery) relative to O2 utilization, necessitating a greater reliance on O2 extraction.

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Acknowledgments

The authors would like to acknowledge the technical assistance of Dr Aaron P. Heenan in collection of the ultrasound blood flow data and Brad Hansen’s overall support in the laboratory. This investigation was supported by Natural Science and Engineering Research Council of Canada.

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None declared.

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Authors and Affiliations

  1. School of Kinesiology, Canadian Centre for Activity and Aging, The University of Western Ontario, London, ON, N6A-3K7, Canada

    Gregory R. duManoir, Darren S. DeLorey, John M. Kowalchuk & Donald H. Paterson

  2. Department of Physiology and Pharmacology, The University of Western Ontario, London, ON, N6A-3K7, Canada

    John M. Kowalchuk

Authors
  1. Gregory R. duManoir
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  2. Darren S. DeLorey
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  3. John M. Kowalchuk
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  4. Donald H. Paterson
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Corresponding author

Correspondence to Donald H. Paterson.

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Communicated by Susan Ward.

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duManoir, G.R., DeLorey, D.S., Kowalchuk, J.M. et al. Kinetics of VO2 limb blood flow and regional muscle deoxygenation in young adults during moderate intensity, knee-extension exercise. Eur J Appl Physiol 108, 607–617 (2010). https://doi.org/10.1007/s00421-009-1263-7

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