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Molecular characterization of the porcine testis-specificphosphoglycerate kinase 2 (PGK2) gene and its association with male fertility

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Abstract

We have isolated and characterized the porcine testis-specific phosphoglycerate kinase 2 (PGK2) gene, and 1665 bp of full-length PGK2 cDNA were also compiled using modified rapid amplification 5′-RACE and 3′-RACE information. The results of genomic and cDNA sequences of the porcine PGK2 gene demonstrated that it is a single-exon intronless gene with a complete open reading frame of 1251 bp encoding a PGK protein of 417 amino acids. Real-time quantitative PCR results showed that PGK2 mRNA was solely expressed in the testis. There was a lower amount of PGK2 expression in the testis of a 10-month-old herniated boar and a very small amount of PGK2 expression in the testis of an 8–week-old cryptorchid piglet compared to an adult boar. Two SNPs in the PGK2 gene (SNP-A: T427C; SNP-B: C914A) resulting in amino acid substitutions (SNP-A: Ser102–Pro102; SNP-B: Thr264–Lys264) were detected and genotyped among six pig breeds. The nucleotide C at SNP-A responsible for the amino acid exchange to proline could lead to the loss of a casein kinase II (CK2) phosphorylation site in the PGK2 peptide. Association analyses between PGK2 genotypes and several traits of sperm quantity and quality were performed. The results showed that SNP-B has a positive significant effect on semen volume in the breed Pietrain (p = 0.08), i.e., boars carrying genotype CC revealed an increased volume of 49 ml compared with boars having the genotype AA.

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Acknowledgments

The authors would like to thank Viola Raupach for expert technical assistance and Wilhelm Wemheuer for fruitful discussions on the results of the association study. The project was funded by a grant of the Erxleben Research & Innovation Council to Bertram Brenig (ERIC-BR1959–2000–02).

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Authors and Affiliations

  1. Institute of Veterinary Medicine, University of Göttingen, Göttingen, Germany

    Kefei Chen, Christoph Knorr, Kesinee Gatphayak & Bertram Brenig

  2. Key Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture, Jiangxi Agricultural University, Nanchang, 330045, Peoples Republic of China

    Kefei Chen

  3. Genetic Solutions Pty Ltd, Albion, 4010, Queensland, Australia

    Gerhard Moser

Authors
  1. Kefei Chen
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  2. Christoph Knorr
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  3. Gerhard Moser
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  4. Kesinee Gatphayak
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  5. Bertram Brenig
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Corresponding author

Correspondence to Bertram Brenig.

Additional information

The nucleotide sequence data reported in this article have been submitted to GenBank and have been assigned the accession numbers AY500132 and AY486962.

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Chen, K., Knorr, C., Moser, G. et al. Molecular characterization of the porcine testis-specificphosphoglycerate kinase 2 (PGK2) gene and its association with male fertility. Mamm Genome 15, 996–1006 (2004). https://doi.org/10.1007/s00335-004-2405-1

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  • DOI: https://doi.org/10.1007/s00335-004-2405-1

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