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Sequence Analysis and Expression of a Blue-light Photoreceptor Gene, Slwc-1 from the Cauliflower Mushroom Sparassis latifolia

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Abstract

Light is a necessary environmental factor for fruit body formation and development of the cauliflower mushroom Sparassis latifolia, a well-known edible and medicinal fungus. In this study, we firstly characterized the SP-C strain, which belonged to S. latifolia. And then we cloned and sequenced a photoreceptor gene (Slwc-1) from S. latifolia. The product of Slwc-1, SlWC-1 (872 aa residues) contained a coiled-coil region, a LOV domain, and two PAS domains. Phylogenetic tree result showed that SLWC-1 was most close to GfWC-1 from Grifola frondosa in edible and medicinal fungus. The Slwc-1 gene was found to be enhanced by light. This report will help to open the still-unexplored field of fruit body development for this fungus.

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Abbreviations

S. latifolia :

Sparassis latifolia

Slwc-1 :

S. latifolia white color-1

LOV:

Light, Oxygen, or Voltage

PAS:

Per-Arnt-Sim

RACE:

Rapid amplification of cDNA ends

ITS:

Internal transcribed spacer

CTAB:

Cetyl-trimethyl ammonium bromide

LED:

Light Emitting Diode

qPCR:

Quantitative real-time PCR

GAPDH:

glyceraldehyde-3-phosphate dehydrogenase

PCR:

Polymerase Chain Reaction

IBS:

Illustrator for biological sequences

References

  1. Dai YC, Wang Z, Binder M, Hibbett DS (2006) Phylogeny and a new species of Sparassis (Polyporales, Basidiomycota): evidence from mitochondrial atp6, nuclear rDNA and rpb2 genes. Mycologia 98(4):584–592

    Article  CAS  PubMed  Google Scholar 

  2. Ohno N, Miura NN, Nakajima M, Yadomae T (2000) Antitumor 1,3-β-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull 23(7):866–872

    Article  CAS  PubMed  Google Scholar 

  3. Harada T, Ohno N (2008) Contribution of dectin-1 and granulocyte macrophage–colony stimulating factor (GM-CSF) to immunomodulating actions of β-glucan. Int Immunopharmacol 8(4):556–566. doi:10.1016/j.intimp.2007.12.011

    Article  CAS  PubMed  Google Scholar 

  4. Yamamoto K, Kimura T, Sugitachi A, Matsuura N (2009) Anti-angiogenic and anti-metastatic effects of β-1,3-D-glucan purified from Hanabiratake, Sparassis crispa. Biol Pharm Bull 32(2):259–263. doi:10.1248/bpb.32.259

    Article  CAS  PubMed  Google Scholar 

  5. Hasegawa A, Yamada M, Dombo M, Fukushima R, Matsuura N, Sugitachi A (2004) Sparassis crispa as biological response modifier. Gan To Kagaku Ryoho 31 (11):1761–1763

    PubMed  Google Scholar 

  6. Hu S, Wang D, Zhang J, Du M, Cheng Y, Liu Y, Zhang N, Wang D, Wu Y (2016) Mitochondria related pathway is essential for polysaccharides purified from Sparassis crispa mediated neuro-protection against glutamate-induced toxicity in differentiated PC12 Cells. Int J Mol Sci 17(2):133. doi:10.3390/ijms17020133

    Article  PubMed Central  Google Scholar 

  7. Chandrasekaran G, Lee YC, Park H, Wu Y, Shin HJ (2016) Antibacterial and antifungal activities of lectin extracted from fruiting bodies of the Korean cauliflower medicinal mushroom, Sparassis latifolia (Agaricomycetes). Int J Med Mushrooms 18(4):291–299. doi:10.1615/IntJMedMushrooms.v18.i4.20

    Article  PubMed  Google Scholar 

  8. Ma L, Lin YQ, Yang C, Ying ZH, Jiang XL (2016) Production of liquid spawn of an edible mushroom, Sparassis latifolia by submerged fermentation and mycelial growth on pine wood sawdust. Sci Hortic 201:22–30

    Article  Google Scholar 

  9. Gong W, Xu R, Xiao Y, Zhou Y, Bian Y (2014) Phenotypic evaluation and analysis of important agronomic traits in the hybrid and natural populations of Lentinula edodes. Sci Hortic 179:271–276

    Article  Google Scholar 

  10. Ying Z, Lin Y, Ma L, Jiang X (2013) Effects of different light quality and quantity on mycelial growth and primordium formation of Sparassis Crispa. Fujian J Agric Sci 6:005 (in Chinese)

    Google Scholar 

  11. Kamada T, Sano H, Nakazawa T, Nakahori K (2010) Regulation of fruiting body photomorphogenesis in Coprinopsis cinerea. Fungal Genet Biol 47(11):917–921

    Article  PubMed  Google Scholar 

  12. Dunlap JC (2006) Proteins in the Neurospora circadian clockworks. J Biol Chem 281(39):28489–28493

    Article  CAS  PubMed  Google Scholar 

  13. Linden H (2002) Circadian rhythms. A white collar protein senses blue light. Science 297(5582):777–778. doi:10.1126/science.1075485

    Article  CAS  PubMed  Google Scholar 

  14. Linden H, Macino G (1997) White collar 2, a partner in blue-light signal transduction, controlling expression of light-regulated genes in Neurospora crassa. EMBO J 16(1):98–109

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Corrochano LM (2007) Fungal photoreceptors: sensory molecules for fungal development and behaviour. Photochem Photobiol Sci 6(7):725–736

    Article  CAS  PubMed  Google Scholar 

  16. He Q, Cheng P, Yang Y, Wang L, Gardner KH, Liu Y (2002) White Collar-1, a DNA binding transcription factor and a light sensor. Science 297(5582):840–843

    Article  CAS  PubMed  Google Scholar 

  17. Hiroaki S, Takatsugu N, Shinya K, Takashi Y, Kazuo S (2007) Sequence analysis and expression of a blue-light photoreceptor gene, Le.phrA from the basidiomycetous mushroom Lentinula edodes. Biosci Biotechnol Biochem 71(9):2206–2213

    Article  Google Scholar 

  18. Ohm RA, Aerts D, Wösten HAB, Lugones LG (2013) The blue light receptor complex WC-1/2 of Schizophyllum commune is involved in mushroom formation and protection against phototoxicity. Environ Microbiol 15(3):943–955

    Article  CAS  PubMed  Google Scholar 

  19. Yang T, Dong C (2014) Photo morphogenesis and photo response of the blue-light receptor gene Cmwc-1 in different strains of C ordyceps militaris. FEMS Microbiol Lett 352(2):190–197

    Article  CAS  PubMed  Google Scholar 

  20. Yang T, Guo M, Yang H, Guo S, Dong C (2015) The blue-light receptor CmWC-1 mediates fruit body development and secondary metabolism in Cordyceps militaris. Appl Microbiol Biotechnol 100(2):743–755

    Article  PubMed  Google Scholar 

  21. Biel SW, Parrish FW (1986) Isolation of DNA from fungal mycelia and sclerotia without use of density gradient ultracentrifugation. Anal Biochem 154(1):21–55

    Article  CAS  PubMed  Google Scholar 

  22. Ryoo R, Sou HD, Ka KH, Park H (2013) Phylogenetic relationships of Korean Sparassis latifolia based on morphological and ITS rDNA characteristics. J Microbiol 51(1):43–48. doi:10.1007/s12275-013-2503-4

    Article  PubMed  Google Scholar 

  23. White TJ, Bruns TD, Lee SB, Taylor JW, Innis MA, Gelfand DH, Sninsky JJ (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protoc 18:315–322

    Google Scholar 

  24. Liu W, Xie Y, Ma J, Luo X, Nie P, Zuo Z, Lahrmann U, Zhao Q, Zheng Y, Zhao Y, Xue Y, Ren J (2015) IBS: an illustrator for the presentation and visualization of biological sequences. Bioinformatics 31(20):3359–3361. doi:10.1093/bioinformatics/btv362

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Jones DT, Taylor WR, Thornton JM (1992) The rapid generation of mutation data matrices from protein sequences. Comput Appl Biosci: CABIOS 8 (3):275–282

    CAS  PubMed  Google Scholar 

  26. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30(12):2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Lin Y, Ma L, Jiang X, Ying Z (2012) Optimization of selected growth parameters for Sparassis crispa. Acta Edulis Fungi 19 (4):35–37

    Google Scholar 

  28. Estrada AF, Avalos J (2008) The White Collar protein WcoA of Fusarium fujikuroi is not essential for photocarotenogenesis, but is involved in the regulation of secondary metabolism and conidiation. Fungal genetics and biology : FG & B 45 (5):705–718. doi:10.1016/j.fgb.2007.12.003

    Article  CAS  Google Scholar 

  29. Idnurm A, Heitman J (2005) Light controls growth and development via a conserved pathway in the fungal kingdom. PLoS Biol 3 (4):e95. doi:10.1371/journal.pbio.0030095

    Article  PubMed  PubMed Central  Google Scholar 

  30. Idnurm A, Rodriguez-Romero J, Corrochano LM, Sanz C, Iturriaga EA, Eslava AP, Heitman J (2006) The Phycomyces madA gene encodes a blue-light photoreceptor for phototropism and other light responses. Proc Natl Acad Sci U S A 103(12):4546–4551. doi:10.1073/pnas.0600633103

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

This work was partially funded by the Special Fund for Scientific Research in the Public Interest of Fujian Province (2016R1019-4), the Science and Technology Innovations Program at Fujian Academy of Agricultural Science (2016PI-44) and Fujian major agro-technique extension service pilot project of edible fungi industry (KNJ-153012).

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    Authors and Affiliations

    1. Institute of Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, 350014, China

      Chi Yang, Lu Ma, Zhenghe Ying, Xiaoling Jiang & Yanquan Lin

    2. National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fujian Academy of Agricultural Sciences, Fuzhou, 350014, China

      Chi Yang, Lu Ma, Zhenghe Ying, Xiaoling Jiang & Yanquan Lin

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    1. Chi Yang
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    Correspondence to Yanquan Lin.

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    Chi Yang and Lu Ma contributed equally to this work.

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    Yang, C., Ma, L., Ying, Z. et al. Sequence Analysis and Expression of a Blue-light Photoreceptor Gene, Slwc-1 from the Cauliflower Mushroom Sparassis latifolia . Curr Microbiol 74, 469–475 (2017). https://doi.org/10.1007/s00284-017-1218-x

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