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Isolation and characterization of polysaccharides with the antitumor activity from Tuber fruiting bodies and fermentation system

  • Biotechnological products and process engineering
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Abstract

Fifty-two polysaccharides were isolated from the fermentation systems of Tuber melanosporum, Tuber indicum, Tuber sinense, Tuber aestivum and the fruiting bodies of Tuber indicum, Tuber himalayense, Tuber sinense by elution with an activated carbon column. Polysaccharides from Tuber fermentation system exhibited relatively higher in vitro antitumor activity against HepG2, A549, HCT-116, SK-BR-3, and HL-60 cells than those from Tuber fruiting bodies. All polysaccharides were mainly composed of d-mannose, d-glucose, and d-galactose, which suggested that the polysaccharides from Tuber fruiting bodies and fermentation system have identical chemical compositions. The results of antitumor activity and structural identification indicated that the polysaccharide fractions could promote antitumor activity. Tuber polysaccharides from Tuber fermentation system exhibited relatively higher than that from Tuber fruiting bodies. These results confirm the potential of Tuber fermentation mycelia for use as an alternative resource for its fruiting bodies.

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Acknowledgments

Financial supports from the National Natural Science Foundation of China (NSFC, Project Nos. 21176059, 21206035, and 21376066), Hubei Provincial Natural Science Foundation for Agriculture (2012DBA20001), High-Tech Industry Development Program for Innovative Research Team in Wuhan Municipality (2013070204020049) are gratefully acknowledged. Ya-Jie Tang also thanks the Chutian Scholar Program (Hubei Provincial Department of Education, China) (2006), Training Program for the Youth Leading Talents by Ministry of Science & Technology, Program for New Century Excellent Talents in University (NCET-11-0961), and Training Program for Top Talents in Hubei Province.

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Authors and Affiliations

  1. Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, 430068, China

    Wei Zhao, Hong-Mei Li & Ya-Jie Tang

  2. Key Laboratory of Biopesticide and Chemical Biology (Ministry of Education), Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Xiao-Hua Wang & Shi-Hua Wang

  3. Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Tao Chen

  4. School of Public Health, Wuhan University, Wuhan, 430071, China

    Zhan-Peng Yuan

Authors
  1. Wei Zhao
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  2. Xiao-Hua Wang
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  3. Hong-Mei Li
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  4. Shi-Hua Wang
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  5. Tao Chen
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  6. Zhan-Peng Yuan
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  7. Ya-Jie Tang
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Corresponding author

Correspondence to Ya-Jie Tang.

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Wei Zhao and Xiao-Hua Wang equally contributed to this work

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Zhao, W., Wang, XH., Li, HM. et al. Isolation and characterization of polysaccharides with the antitumor activity from Tuber fruiting bodies and fermentation system. Appl Microbiol Biotechnol 98, 1991–2002 (2014). https://doi.org/10.1007/s00253-013-5379-7

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  • DOI: https://doi.org/10.1007/s00253-013-5379-7

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