[go: up one dir, main page]
Skip to main content

Advertisement

SpringerLink
Log in

Effect of thymoquinone, a constituent of Nigella sativa L., on ischemia–reperfusion in rat skeletal muscle

  • Original Article
  • Published:
Naunyn-Schmiedeberg's Archives of Pharmacology Aims and scope Submit manuscript

Abstract

Thymoquinone have been shown to decrease ischemia–reperfusion injury (IRI) in some tissues such as gastric mucosa and brain. In this study, the effect of thymoquinone was evaluated on an animal model of IRI in the rat hind limb. Hind limb ischemia was induced by clamping the common femoral artery and vein. After 2 h ischemia, the clamp on the femoral vessels was taken off and the animal underwent 1 h reperfusion. Muscle injuries were evaluated by recording the electromyographic (EMG) potentials and performing some biochemical analysis including thiobarbituric acid reactive substances (TBARS), total sulfhydryl (SH) groups, and antioxidant capacity of muscle using ferric reducing ability of plasma (FRAP) assay. Ischemia was induced using free-flap surgery in skeletal muscle. Thymoquinone (20, 40 and 80 mg/kg) and normal saline (10 ml/kg) were administered intraperitoneally 1 h prior to reperfusion. The average peak-to-peak amplitude during ischemic reperfusion was significantly increased in thymoquinone groups in comparison with the control group. Following thymoquinone administration, the total SH contents and antioxidant capacity were elevated in muscle flap. The malondialdehyde (MDA) level was declined significantly in test groups. It is concluded that thymoquinone have some protective effects against the muscle tissue injury caused by lower limb ischemia–reperfusion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Subscribe and save

Springer+ Basic
$34.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Abdel-Fattah AFM, Matsumoto K, Watanabe H (2000) Antinociceptive effects of Nigella sativa oil and its major component, thymoquinone, in mice. Eur J Pharmacol 400:89–97

    Article  PubMed  CAS  Google Scholar 

  • Abdollahi M, Ranjbar R, Shadnia S, Nikfar S, Rezaie A (2004) Pesticides and oxidative stress: a review. Med Sci Mon 10:141–147

    Google Scholar 

  • Al-Gharably NM, Badary OA, Nagi MN, Al-Sawaf HA, Al-Rikabi AC, Al-Bekairi AM (1997) Protective effect of thymoquinone against carbon tetrachloride-induced hepatotoxicity in mice. Res Commun Pharmacol Toxicol 2:41–50

    CAS  Google Scholar 

  • Awad AS, Kamel R, Sherief MA (2011) Effect of thymoquinone on hepatorenal dysfunction and alteration of CYP3A1 and spermidine/spermine N-1-acetyl-transferase gene expression induced by renal ischaemia–reperfusion in rats. J Pharm Pharmacol 63:1037–1042

    Article  PubMed  CAS  Google Scholar 

  • Benzie IFF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: the FRAP assay. Anal Biochem 239:70–76

    Article  PubMed  CAS  Google Scholar 

  • Benzie IFF, Strain JJ (1999) Ferric reducing/antioxidant power assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Method Enzymol 299:15–27

    Article  CAS  Google Scholar 

  • Blaisdell FW (2002) The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review. Cardiovasc Surg 10:620–630

    Article  PubMed  Google Scholar 

  • Burits M, Bucar F (2000) Antioxidant activity of Nigella sativa essential oil. Phytother Res 14:323–328

    Article  PubMed  CAS  Google Scholar 

  • Daba MH, Abdel-Rahman MS (1998) Hepatoprotective activity of thymoquinone in isolated rat hepatocytes. Toxicol Lett 95:23–29

    Article  PubMed  CAS  Google Scholar 

  • El-Abhar HS, Abdallah M, Saleh S (2003) Gastroprotective activity of Nigella sativa oil and its constituent, thymoquinone, against gastric mucosal injury induced by ischaemia–reperfusion in rats. J Ethnopharmacol 84:251–258

    Article  PubMed  CAS  Google Scholar 

  • El-Dakhakhny M, Mady NI, Halim MA (2000) Nigella sativa L. protects against induced hepatotoxicity and improves serum lipid profile in rats. Arzneimittelforsch 50:832–836

    PubMed  CAS  Google Scholar 

  • Ellman G (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77

    Article  PubMed  CAS  Google Scholar 

  • Fernandez J, Perez-Alvarez JA, Fernandez-Lopez JA (1997) Thiobarbituric acid test for monitoring lipid oxidation in meat. Food Chem 99:345–353

    Article  Google Scholar 

  • Fernandez DC, Antuna SS, Martinez E, Arias AP (2000) Ischemia–reperfusion injury in muscular flaps: role of superoxide dismutase and catalase, 6th Internet World Congress for Biomedical Sciences, INABIS, Presentation# 159

    Google Scholar 

  • Grisotto PC, dos Santos AC, Coutinho-Neto J, Cherry J, Picinatto CE (2000) Indicators of oxidative injury and alterations of the cell membrane in the skeletal muscle of rats submitted to ischemia and reperfusion. J Surg Res 92:1–6

    Article  PubMed  CAS  Google Scholar 

  • Gute DC, Ishida T, Yarimizu K, Korthuis RJ (1998) Inflammatory responses to ischemia and reperfusion in skeletal muscle. Mol Cell Biochem 179:169–187

    Article  PubMed  CAS  Google Scholar 

  • Hamdy NM, Taha RA (2009) Effects of Nigella sativa oil and thymoquinone on oxidative stress and neuropathy in streptozotocin-induced diabetic rats. Pharmacology 84:127–134

    Article  PubMed  CAS  Google Scholar 

  • Hassan M, El-Dakhakhny M (1992) Effect of some Nigella sativa constituents on chemical carcinogenesis in hamster cheek pouch. J Egypt Soc Pharmacol Exp Ther 11:675–677

    Google Scholar 

  • Hosseinzadeh H, Hosseini A, Nassiri-Asl M, Sadeghnia HR (2007a) Effect of Salvia leriifolia Benth. root extracts on ischemia–reperfusion in rat skeletal muscle. BMC Complement Alter Med 7:23

    Article  Google Scholar 

  • Hosseinzadeh H, Moghim FF, Mansouri SMT (2007b) Effect of Nigella sativa seed extracts on ischemia–reperfusion in rat skeletal muscle. Pharmacol gyonline 2:326–335

    Google Scholar 

  • Hosseinzadeh H, Parvardeh S, Asl MN, Sadeghnia HR, Ziaee T (2007c) Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cerebral ischemia–reperfusion injury in rat hippocampus. Phytomedicine 14:621–627

    Article  PubMed  CAS  Google Scholar 

  • Hosseinzadeh H, Modaghegh MH, Saffari Z (2009) Crocus sativus L. (saffron) extract and its active constituents (crocin and safranal) on ischemia–reperfusion in rat skeletal muscle. eCAM 6:343–350

    PubMed  Google Scholar 

  • Houghton PJ, Zarka R, De-Las-Heras B, Hoult JR (1995) Fixed oil of Nigella sativa and derived thymoquinone inhibit Eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med 61:33–36

    Article  PubMed  CAS  Google Scholar 

  • Huang D, Ou B, Prior RL (2005) The chemistry behind antioxidant capacity assays. J Agric Food Chem 53:1841–1856

    Article  PubMed  CAS  Google Scholar 

  • Iida H, Schmelzer JD, Schmeichel AM, Wang Y, Low PA (2003) Peripheral nerve ischemia: reperfusion injury and fiber regeneration. Exp Neurol 184:997–1002

    Article  PubMed  Google Scholar 

  • Ismail M, Al-Naqeep G, Chan KW (2010) Nigella sativa thymoquinone-rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats. Free Radic Biol Med 48:664–672

    Article  PubMed  CAS  Google Scholar 

  • Korthals JK, Maki T, Gieron MA (1985) Nerve and muscle vulnerability to ischemia. J Neurol Sci 71:283–290

    Article  PubMed  CAS  Google Scholar 

  • Mahgoub AA (2003) Thymoquinone protects against experimental colitis in rats. Toxicol Lett 143:133–143

    Article  PubMed  CAS  Google Scholar 

  • Mansour MA, Nagi MN, El-Khatib AS, Al-Bekairi AM (2002) Effects of thymoquinone on antioxidant enzyme activities, lipid peroxidation and DT-diaphorase in different tissues of mice: a possible mechanism of action. Cell Biochem Funct 20:143–151

    Article  PubMed  CAS  Google Scholar 

  • Mihara M, Uchiyama M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 86:271–278

    Article  PubMed  CAS  Google Scholar 

  • Nagamatsu M, Schmelzer JD, Zollman PJ, Smithson IL, Nickander KK, Low PA (1996) Ischemic reperfusion causes lipid peroxidation and fiber degeneration. Muscle Nerve 19:37–47

    Article  PubMed  CAS  Google Scholar 

  • Nagi MN, Alam K, Badary OA, Al-Shabaneh OA, Al-Sawaf HA, Al-Bekairi AM (1999) Thymoquinone protects against carbon tetrachloride hepatotoxicity in mice via an antioxidant mechanism. Biochem Mol Biol Int 47:153–159

    PubMed  CAS  Google Scholar 

  • Oguzhanoglu A, Kurt T, Sahiner T (2000) Nerve conduction parameters during ischemia–reperfusion in the rat sciatic nerve. Electromyogr Clin Neurophysiol 40:487–490

    PubMed  CAS  Google Scholar 

  • Ossowska K, Lorence-Koci E, Schulze G, Wolfarth S (1996) The influence of dizolcipine (MK-801) on the reserpine-enhanced electromyographic stretch reflex in rats. Neurosci Lett 203:73–76

    Article  PubMed  CAS  Google Scholar 

  • Parihar MS, Hemnani T (2003) Phenolic antioxidants attenuate hippocampal neuronal cell damage against kainic acid-induced excitotoxicity. J Biosci 28:121–128

    Article  PubMed  CAS  Google Scholar 

  • Schmelzer JD, Zochodne DW, Low PA (1989) Ischemic and reperfusion injury of rat peripheral nerve. Proc Natl Acad Sci USA 86:1639–1342

    Article  PubMed  CAS  Google Scholar 

  • Soszynski M, Bartosz G (1997) Decrease in accessible thiols as an index of oxidative damage to membrane proteins. Free Rad Biol Med 23:463–469

    Article  PubMed  CAS  Google Scholar 

  • Woo CC, Loo SY, Gee V, Yap CW, Sethi G, Kumar AP, Benny Tan KH (2011) Anticancer activity of thymoquinone in breast cancer cells: possible involvement of PPAR-γ pathway. Biochem Pharmacol. in press

  • Woodruff TM, Arumugam TV, Shiels IA, Reid RC, Fairlie DP, Taylor SM (2004) Protective effects of a potent C5a receptor antagonist on experimental acute limb ischemia–reperfusion in rats. J Surg Res 116:81–90

    Article  PubMed  CAS  Google Scholar 

  • Worthen DR, Ghosheh OA, Crooks PA (1998) The in vitro anti-tumor activity of some crude and purified components of blackseed Nigella sativa L. Anticancer Res 18:1527–1532

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

The authors are thankful to the Vice Chancellor of Research, Mashhad University of Medical Sciences for financial support. The results described in this paper are part of a Pharm. D. thesis.

Author information

Authors and Affiliations

  1. Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, 1365-91775, Mashhad, I.R. Iran

    Hossein Hosseinzadeh

  2. School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, I. R. Iran

    Samaneh Taiari

  3. Department of Pharmacology, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, I.R. Iran

    Marjan Nassiri-Asl

Authors
  1. Hossein Hosseinzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Samaneh Taiari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marjan Nassiri-Asl
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hossein Hosseinzadeh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hosseinzadeh, H., Taiari, S. & Nassiri-Asl, M. Effect of thymoquinone, a constituent of Nigella sativa L., on ischemia–reperfusion in rat skeletal muscle. Naunyn-Schmiedeberg's Arch Pharmacol 385, 503–508 (2012). https://doi.org/10.1007/s00210-012-0726-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00210-012-0726-2

Keywords

Search

Navigation