Summary
An immunofluorescence test on smears of nasal epithelial cells was used to detect coronavirus infection in the respiratory tract of calves. Thirteen gnotobiotic calves were infected with coronavirus isolates derived from faeces or respiratory material: virus was detected in faeces and nasal swabs from all animals. In 115 calves from a field survey, there was a significant association between coronavirus excretion from both respiratory and enteric routes in calves with diarrhoea. In a further 12 calves, at necropsy, the predilection sites for coronavirus growth were the distal small intestine, large intestine and the epithelia of the nasal cavity and trachea. Antigen was not found in lung tissue by immunofluoresence or immunoperoxidase staining.
Infection with enteric coronavirus induced immunity to reinfection and to heterologous challenge with two coronavirus isolates derived from the respiratory tract. Nine coronaviruses were cultivated, cloned and antisera to three were prepared in pigs. There was complete virus neutralisation in tests with homologous sera and significant cross reactions with the eight other isolates which were of intestinal and respiratory origin. Thus, these bovine coronavirus isolates belonged to the same serotype despite the source of virus.
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Bridger, J. C., Woode, G. N., Meyling, A.: Isolation of a coronavirus from neonatal calf diarrhoea in Great Britain and Denmark. Vet. Microbiol.3, 101–113 (1978).
Dennis, M. J., Davies, D. C., Hoare, M. N.: A simplified apparatus for the microbiological isolation of calves. Brit. Vet. J.132, 642–646 (1976).
Doughri, A. M., Storz, J.: Light and ultrastructural pathologic changes in intestinal coronavirus infection of newborn calves. Zentralblatt für Veterinärmed. B24, 367–385 (1977).
Hall, G. A., Bridger, J. C., Brooker, B. E., Parsons, K. R., Ormerod, E.: Lesions of gnotobiotic calves experimentally infected with a calicivirus like (Newbury) agent. Vet. Pathol.21, 208–215 (1984).
Haralambiev, H. E., Miltov, B. K., Popov, G. V.: Isolation of bovine coronavirus and rotaviruses in organ cultures from respiratory mucosa. Compt. Rend. de l'Acad. Bulgar. Sci.32, 1597–1600 (1979).
Laude, H., Charley, B., Gelfi, J.: Replication of transmissible gastroenteritis coronavirus (TGEV) in swine alveolar macrophages. J. gen. Virol.65, 327–332 (1984).
Laporte, J., Bobulesco, P., Rossi, F.: Une Lignée cellulaire particulierement sensible à la replication due coronavirus enteritique bovin. Compt. Rend. de l'Acad. Sci. (Paris)290, 623–626 (1980).
McIntosh, K., McQuillan, J., Reed, S. E., Gardner, P. S.: Diagnosis of human coronavirus infection by immunofluorescence: method and application to respiratory disease in hospitalised children. J. med. Virol.2, 341–346 (1978).
McNulty, M. S., Bryson, D. G., Allan, G. M., Logan, E. F.: Coronavirus infections of the bovine respiratory tract. Vet. Microbiol.9, 425–439 (1984).
Mebus, C. A., Stair, E. L., Rhodes, M. B., Twiehaus, M. J.: Pathology of neonatal calf diarrhoea induced by a coronavirus-like agent. Vet. Pathol.10, 45–64 (1973).
Parsons, K. R., Wilson, A. M., Hall, G. A., Bridger, J. C., Chanter, N. C., Reynolds, D. J.: Localisation of enteropathogens in paraffin embedded tissue by immunoperoxidase. J. Clin. Pathol.37, 645–650 (1984).
Patel, J. R., Davies, H. A., Edington, N., Laporte, J., Macnaughton, M. R.: Infection of a calf with the enteric coronavirus strain Paris. Arch. Virol.73, 319–327 (1982).
Pocock, D. H., Garwes, D. J.: The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. J. gen. Virol.37, 487–499 (1977).
Reed, S. E.: Coronavirus infections of the respiratory tract. Proceedings of the Fourth Munich Symposium on Microbiology, Munich, 201–216. 1979.
Reynolds, D. J.: Coronavirus replication in the intestinal and respiratory tract during infection of calves. Ann. Recherche Vétérin.14, 445–446 (1983).
Reynolds, D. J., Thomas, L. H., Bridger, J. C., Stott, E. J.: Coronavirus infections of cattle. Fifth International Congress of Virology, Strasbourg, 1981.
Reynolds, D. J., Chasey, D., Scott, A. C., Bridger, J. C.: Evaluation of ELISA and electron microscopy for the detection of coronavirus and rotavirus in bovine faeces. Vet. Rec.114, 397–401 (1984).
Sprino, P. J., Ristic, M.: Intestinal, pulmonary and serum antibody response to transmissible gastroenteritis virus by the oral-nasal routes of inoculation. Amer. J. Vet. Res.43, 255–261 (1982).
Storz, R., Rott, R., Kaluza, G.: Enhancement of plaque formation and cell fusion of an enteropathogenic coronavirus by trypsin treatment. Infect. Immun.31, 1214–1222 (1981).
Stott, E. J., Thomas, L. H., Bridger, J. C., Jebbett, N. J.: Replication of a bovine coronavirus in organ cultures of foetal trachea. Vet. Microbiol.1, 65–69 (1976).
Stott, E. J., Garwes, D. J.,: Respiratory coronaviruses, 7th ed. London: Topley and Wilson 1984.
Thomas, L. H., Stott, E. J.: Comparison of three methods for sampling the bovine upper respiratory tract for viruses. Res. Vet. Sci.18, 227–229 (1975).
Thomas, L. H., Stott, E. J.: Diagnosis of respiratory syncytial virus in the bovine respiratory tract by immunfluorescence. Vet. Rec.108, 432–435 (1981).
Thomas, L. H., Gourlay, R. N., Stott, E. J., Howard, C. J., Bridger, J. C.: A search for new microorganisms in calf pneumonia by the inoculation of gnotobiotic calves. Rev. Vet. Sci.33, 170–182 (1982).
Toth, T. E.: Trypsin-enhanced replication of neonatal calf diarrhoea coronavirus in bovine embryonic lung cells. Amer. J. Vet. Res.43, 967–972 (1982).
Underdahl, N. R., Mebus, C. A., Stair, E. L., Rhodes, M. D., McGill, L. D., Twiehaus, M. J.: Isolation of transmissible gastroenteritis virus from lungs of market weight swine. Amer. J. Vet. Res.35, 1209–1216 (1974).
Wege, H., Siddell, S., Termeulen, V.: The biology and pathogenesis of coronaviruses. Curr. Top. Microbiol. Immunol.99, 165–200 (1982).
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Reynolds, D.J., Debney, T.G., Hall, G.A. et al. Studies on the relationship between coronaviruses from the intestinal and respiratory tracts of calves. Archives of Virology 85, 71–83 (1985). https://doi.org/10.1007/BF01317007
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DOI: https://doi.org/10.1007/BF01317007