Abstract
In the present study, we have assayed the enzymatic activity of Cu,Zn−SOD, Mn−SOD, GSH−Px, GSH-Red, Cat, and G6PD in rat retina as a function of age. Conjugated diene levels and MDA formation were also determined. The conjugated diene levels in rat retina were found to increase significantly with age, accompanied by a marked decrease in GSH−Px and Cat activities. No agerelated change in MDA levels and in GSH-Red and G6PD activity was found, whereas a significant increase in SOD activity was observed between 1 and 4 months. Decreased GSH−Px and Cat activity is related to increased lipid peroxidation with age.
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Sickel, W. 1972. handbook of Sensory Physiology. Pages 667–727,in Fuortes, M. G. F. (ed.), Retinal metabolism in dark and light, Springer-Verlag, Berlin.
Crapo, D. J., and Tierney, D. F. 1974. Superoxide dismutase and pulmonary oxygen toxicity. Am. J. Physiol., 226:1401–1407.
Freeman, B. A., and Crapo, J. D. 1982. Free radicals and tissue injury. Lab. Invest., 47:412–426.
Varma, S. D., and Richards, R. D. 1988. Cellular antioxidant defense mechanisms. Pages 71–88,in Chow, C. K. (ed.), CRC Press, Inc. Boca Raton, Florida.
Halliwell, B., and Gutteridge, M. C. 1984. Oxygen toxicity, oxygen radical, transition metals and disease. Biochem. J. 219:1–14.
Heath, H. 1962. The distribution and possible functions of ascorbic acid in the eye. Exp. Eye Res. 1:362–367.
Dilley, R. A., and McConnell, D. G. 1970. Alpha-tocopherol in the retinal outer segment of bovine eyes. J. Membr. Biol. 2:317–323.
Farnsworth, C. C., and Dratz, E. A. 1976. Oxidative damage of retinal rod outer segment membranes and the role of vitamin E. Biochim. Biophys. Acta 443:556–570.
Katz, M. L., Robison, W. G. Jr., and Dratz, E. A. 1984. Free radicals in molecular biology, Aging, and Disease. Pages 163–180,in Armstrong, D., Sohal, R. S., Cutler, R. G., and Slater, T. F. (eds.), Potential role of autoxidation in age changes of the retina and retinal pigment epithelium of the eye, Raven Press, New York.
Hunt, D. F., Organisciak, D. T., Wang, H. M., and Wu, R. L. C. 1984. α-tocopherol in the developing rat retina: a high pressure liquid chromatographic analysis. Curr. Eye Res. 3:1281–1288.
Reim, M., Heuvels, B., and Cattepoel, H. 1974. Glutathione peroxidase in some ocular tissues. Ophthalmic Res. 6:228–234.
Fried, R., and Mandel, P. 1975. Superoxide dismutase of mammalian nervous system. J. Neurochem. 24:433–438.
Hall, M. O., and Hall, D. O. 1975. Superoxide dismutase of bovine of frog outer segments. Biochem. Biophys. Res. Commun. 67:1199–1204.
Bhuyan, K. C., and Bhuyan, D. K. 1977. Regulation of hydrogen peroxide in eye humors. Effect of 3-amino-1-H-1,2,4-triazole on catalase and glutathione peroxidase of rabbit eye. Biochim. Biophys. Acta 497:641–651.
Flohe, L. 1979. Oxygen free radicals and tissue damage. CIBA Foundation Symposium. 65. Pages 95–122. Glutathione peroxidase: fact and fiction, Excerpta Medica, Amsterdam.
Kagan, V. E., Lankin, V. Z., Shvedova, A. A., Novikov, K. N., Dobrina, S. K., Bratkovskaya, L. B., and Kuliev, I. Y. 1979. Enzymic and nonenzymic systems protecting photoreceptor membranes against active forms of oxygen and lipid peroxides. Bull. Exp. Biol. Med. 88:856–858.
Stone, W. L., and Dratz, E. A. 1982. Selenium and non-selenium glutathione peroxidase activities in selected ocular and non-ocular rat tissues. Exp. Eye Res. 35:405–412.
Bensinger, R. E., Crabb, J. W., and Johnson, C. M. 1982. Purification and properties of superoxide dismutase from bovine retina. Exp. Eye Res. 34:623–634.
Naash, M. I., and Anderson, R. E. 1984. Characterization of glutathione peroxidase in frog retina. Curr. Eye Res. 3:1299–1304.
Rao, N. A., Thaete, L. G., Delmage, J. M., and Sevanian, A. 1985. Superoxide dismutase in ocular structures. Invest. Ophtalmol. Vis. Sci. 26:1778–1781.
McCord, J., and Fridovich, I. 1969. Superoxide dismutase an enzymatic function for erythrocuprein. J. Biol. Chem. 244:6049–6055.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. G. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.
Paglia, O. E., Valentine, W. N. 1967. Studies on the quantitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70:158–169.
Horn, H. D. 1963. Methods of enzymatic analysis. Pages 875–879in Bergmeyer, H. U. (ed.), Glutathione reductase. Academic Press, New York.
Holmes, R. S., and Masters, C. J. 1969. On the tissue and subcellular distribution of multiple forms of catalase in the rat. Biochem. Biophys. Acta, 191, 488–490.
Kornberg, A., and Horecker, B. L. 1955. Methods of enzymology. Pages 323–325in Colowich, S. P., and Kaplan, N. A. (ed.), New York, Academic Press.
Recknagel, R. O., and Glende, E. A. 1984. Methods in enzymology. Pages 331–337in Packer, L. (ed.), Spectrophotometric detection of lipid conjugated dienes. New York, Academic Press.
Henry, R. J. 1964. Clinical Chemistry, principles and technics. Page 838. Harper and Row Publishers.
Ohkawa, H., Ohishi, N., and Yagi, K. 1979. Assay for lipid peroxydes in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351–358.
Handelman, G. J., and Dratz, E. A. 1986. Oxidant damage to retina and pigment epithelium. Advanc. in Free Rad. Biol. Med. 2:1–89.
Vanella, A., Geremia, E., D'Urso, G., Tiriolo, P., Di Silvestro, I., Grimaldi, R., and Pinturo, R. 1982. Superoxide dismutase activities in aging rat brain. Gerontology 28:108–113.
Nohl, H., and Hegner, D. 1978. Do mitochondria produce oxygen radical in vivo? Eur. J. Biochem. 82:563–567.
Vanella, A., Villa, R. F., Gorini, A., Campisi, A., Giuffrida-Stella, A. M. 1989. Superoxide dismutase and cytochrome oxidase activities in light and heavy synaptic mitochondria from cerebral cortex during aging. J. Neurosci. Res., 22:351–355.
Kagan, V., Shvedova, A. A., Novikov, K. N., and Kozlov, Y. P. 1973. Light-induced free radical oxidation of membrane lipids in photoreceptors of frog retina. Biochim. Biophys. Acta 330:76–79.
Farnsworth, C. C., Stone, W. L., and Dratz, A. 1978. Effects of Vitamin E and selenium deficiency on the fatty acid composition of rat retinal tissues. Biochim. Biophys. Acta, 552:281–293.
Noell, W. K. 1980. The effects of constant light on visual processes. Pages 3–28in Williams, T. P., and Baker, B. N., (eds.), There are different kinds of retinal light damage in the rat. New York, Plenum Press.
Parrish, J. A., Anderson, R. R., Urbach, F., and Pitts, D. 1978. UV-A. Biological effects of ultraviolet radiation with emphasis on human responses to longwave ultraviolet. New York, Plenum Press.
Sarna, T., and Sealy, R. C. 1984. Free radicals from eumelanins: quantum yields and wavelength dependence. Arch. Biochem. Biophys. 232:574–578.
Goodchild, N. T., Kwock, L., and Lin, P. S. 1981. Oxygen and oxy-radicals in chemistry and biology. Pages 654–648in Rodgers, M. A. J., Powers, E. L., (eds.) Melanin: a possible superoxide scavenger. New York, Academic Press.
Geremia, E., Corsaro, C., Bonomo, R., Giardinelli, R., Pappalardo, P., Vanella, A., and Sichel, G. 1984. Eumelanins as free radical trap and superoxide dismutase activities in amphibia. Comp. Biochem. Physiol. 79B:67–69.
Noell, W. K. 1980. Possible mechanism of photoreceptor damage by light in mammalian eye. Vis. Res. 20:1163.
Pacer, Z., Veselkova, A., and Rath, R. 1965. Kinetik des malondialehydes im organismus. Experentia. 21:19–20.
Armstrong, D., Santangelo, G., and Connole, E. 1981. The distribution of peroxide regulating enzymes in canine eye. Curr. Eye Res. 1:225–242.
Vanella, A., Geremia, E., D'urso, G., Tiriolo, P., Di Silvestro, I., Grimaldi, E., and Pinturo, R. 1980. Superoxide dismutase activities in aging rat brain. It. J. Biochem. 29:6–7.
Benzi, G., Marzatico, F., Pastoris, O., and Villa, R. F. 1989. Relationship between aging, drug treatment and the cerebral enzymatic antioxidant system. Exp. Gerontol. 24:137–148.
Del Maestro, R., and McDonald, W. 1989. Subcellular localization of superoxide dismutases, glutathione peroxidase and catalase in developing rat cerebral cortex. Mech. Aging Dev. 48:15–31.
Mishra, O. P., and Delivoria-Papadopoulos, M. 1988. Antioxidant enzymes in fetal guinea pig brain during development and the effect of maternal hypoxia. Dev. Brain Res. 42:173–179.
Mishra, O. P., and Delivoria-Papadopoulos, M. 1989. Lipid peroxidation in developing fetal guinea pig brain during nonnoxia and hypoxia. Dev. Brain Res. 45:129–135.
Benzi, G., Pastoris, O., Marzatico, F., and Villa, R. F. 1989. Age-related effect induced by oxidative stress on the cerebral glutathione system. Neur. Res. 14:473–481.
Mizuno, Y., and Ohta, K. 1986. Regional distribution of thiobarbituric acid-reactive products, activities of enzymes regulating the metabolism of oxygen free radicals and some of the related enzymes in adult and aged rat brains. 1986. J. Neurochem. 46:1344–1352.
Benzi, G., Pastoris, O., Marzatico, F., and Villa, R. F. 1989. Cerebral enzyme antioxidant system. Influence of aging and phosphatidylcholine. J. Cerebr. Blood Flow Metab. 9:373–380.
Martin, J. P., and Burch, P. E. 1988. Oxy-radicals in molecular biology and pathology. Pages 393–404,in Cerutti, P. A., Fridovich, I., and McCord, J. M. (eds.), Oxygen radicals are generated by dye-mediated intracellular photooxidation. Alan R. Liss, Inc., New York.
Noell, W. K., Walker, V., Kamy, B., and Berman, S. 1966. Retinal damage by light in rats. Invest. Ophthalmol. 5:450–473.
Kuwabara, T., and Gorn, R. A. 1968. Retinal damage by visible light: an electron microscopic study. Arch. Ophthalmol. 79:69–78.
Autor, A. P., Frank, L., and Roberts, R. J. 1976. Developmental characteristic of pulmonary superoxide dismutase: relationship to idiopathic respiratory distress syndrome Pediatr. Res. 10:154–158.
Stevens, J. B., and Autor, A. P. 1977. Induction of superoxide dismutase by oxygen neonatal rat lung. J. Biol. Chem. 252:3509–3514.
Vanella, A., Pinturo, R., Grimaldi, R., Tiriolo, P., Di Silvestro, I., Grasso, M., D'Urso, G., and Geremia, E. 1981. Superoxide dismutase activities in rat brain: effect of hyperoxia. IRCS Med. Sci. 9:144–145.
Vanella, A., Geremia, E., Tiriolo, P., Pinturo, R., and Rizza, V. 1982. Advances in studies on heart metabolism. Pages 449–454in Caldarera, C. M., Harris, P. (eds.), Superoxide dismutase activites in rat heart: effect of hyperoxia. Bologna, CLUEB.
Stone, W. L., Henderson, R. A., Howard, G. H., Hollis, A. L., Payne, P. H., and Scott, R. L. 1989. The role of antioxidant nutrients in preventing hyperbaric oxygen damage to the retina. Free Rad. Biol. Med. 6:505–512.
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Castorina, C., Campisi, A., Di Giacomo, C. et al. Lipid peroxidation and antioxidant enzymatic systems in rat retina as a function of age. Neurochem Res 17, 599–604 (1992). https://doi.org/10.1007/BF00968789
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DOI: https://doi.org/10.1007/BF00968789