Abstract
The development of drug delivery systems, sensors and other devices based on liposomes (small unilamellar lipid vesicles, SUVs) requires the adsorption of intact lipid structures onto solid surfaces in the first place. In this work, we report on the in situ investigation of the adsorption of liposomes of 1,2-dipalmitoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (DPPG) onto a rough surface by neutron reflectivity. Rough surfaces are achieved by preparing polyelectrolyte layer-by-layer films, which act as soft polymer cushions. Neutron reflectivity measurements performed at the solid/D2O interface allow for the determination of the thickness of the adsorbed structures. The conducted investigation proofs that the liposomes dispersed in the liquid phase are generally adsorbed intact onto the cushion surface, confirming that the roughness of the latter is a variable to be taken into account if one intends to adsorb intact lipid structures. Liposome flattening is observed and justified by the attractive electrostatic interactions occurring between the negatively charged lipid liposomes and the outermost, positively charged polyelectrolyte layer of the cushion. The conducted measurements further demonstrate that the adsorbed liposomes are stable for several hours. These findings are fundamental for the development of devices based on immobilized but intact SUVs on sensor surfaces.
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References
Luisi, P.L., Ferri, F., Stano, P.: Approaches to semi-synthetic minimal cells: a review. Naturwissenschaften 93(1), 1–13 (2006)
Lukyanov, A.N., Torchilin, V.P.: Micelles from lipid derivatives of water-soluble polymers as delivery systems for poorly soluble drugs. Adv. Drug Deliv. Rev. 56(9), 1273–1289 (2004)
Akbarzadeh, A., et al.: Liposome: classification, preparation, and application. Nanoscale Res. Lett. 8, 102 (2013). https://doi.org/10.1186/1556-276x-8-102
Jesorka, A., Orwar, O.: Liposomes: technologies and analytical applications. Ann. Rev. Anal. Chem. 1, 801–832 (2008)
Pires, F., Duarte, A., Ferreira, Q., Magalhães-Mota, G., Ribeiro, P.A., Raposo, M.: Imaging of liposomal drug delivery systems by atomic force microscopy. In: Méndez-Vilas, A. (ed.) Microscopy and Imaging Science: Practical Approaches to Applied Research and Education. pp. 183–194. Formatex Research Center (2017). http://www.microscopy7.org/
Haas, H., Raposo, M., Ribeiro, P., Steitz, R., Cavatorta, P., Riccio, P.: Myelin model membranes on a soft polymer cushion. Biophys. J. (Annual Meeting Abstracts), 80(1), Part 2, 23A–24A (2001)
Duarte, A.A., et al.: DPPG liposomes adsorbed on polymer cushions: effect of roughness on amount, surface composition and topography. J. Phys. Chem. B. 119(27), 8544–8552 (2015)
Howse, J.R., Manzanares-Papayanopoulos, E., McLure, I.A., Bowers, J., Steitz, R., Findenegg, G.H.: Adsorption from alkane+perfluoroalkane mixtures at fluorophobic and fluorophilic surfaces. II. Crossover from critical adsorption to complete wetting. J. Chem. Phys. 116, 7177 (2002)
Steitz, R., Leiner, V., Siebrecht, R., Klitzing, R.: Influence of the ionic strength on the structure of polyelectrolyte films at the solid-liquid interface. Colloids Surf. A Physicochem. Eng. Aspects 163, 63–70 (2000)
Parrat, L.G.: Surface studies of solids by total reflection of X-rays. Phys. Rev. 95, 359 (1954)
Russel, T.P.: X-ray and neutron reflectivity for the investigation of polymers. Mater. Sci. Rep. 5, 171–271 (1990)
Ribeiro, P.A., Steitz, R., Lopis, I.E., Haas, H., Oliveira Jr., O.N., Raposo, M.: Thermal stability of poly(o-methoxyaniline) layer-by-layer films investigated by neutron reflectivity and UV-VIS spectroscopy. J. Nanosci. Nanotechnol. 6, 1396–1404 (2006)
Zaraiskaya, T., Jeffrey, K.R.: Molecular dynamics simulations and 2H NMR study of the GalCer/DPPG lipid bilayer. Biophys. J. 88, 4017–4031 (2005)
Pimthon, J., Willumeit, R., Lendlein, A., Hofmann, D.: All-atom molecular dynamics simulation studies of fully hydrated gel phase DPPG and DPPE bilayers. J. Mol. Struct. 921, 38–50 (2009)
Magro, C., Mateus, E., Raposo, M., Ribeiro, A.: Overview of electronic tongue sensing in environmental aqueous matrices: potential for monitoring emerging organic contaminants. Environ. Rev. (2018). http://dx.doi.org/10.1139/er-2018-0019
Acknowledgments
The authors acknowledge the V6 reflectometer facility at the Berlin Neutron Scattering Center (BENSC), Helmholtz Zentrum Berlin für Materialien und Energie (former Hahn-Meitner-Institut), Berlin, Germany and for their support through the Access to Research Infrastructure action. The authors acknowledge financial support from FEDER, through Programa Operacional Factores de Competitividade − COMPETE and Fundação para a Ciência e a Tecnologia − FCT, by the project PTDC/FIS-NAN/0909/2014 and the Portuguese research Grant No. PEst-OE/FIS/UI0068/2011 and UID/FIS/00068/2013 through FCT-MEC (Portugal) and by FAPESP and CNPq (Brazil).
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Raposo, M., Duarte, A.A., Gomes, P.J., Ribeiro, P.A., Moraes, M.L., Steitz, R. (2019). Structural Features of a DPPG Liposome Layer Adsorbed on a Rough Surface. In: Bartoletti, M., et al. Computational Intelligence Methods for Bioinformatics and Biostatistics. CIBB 2017. Lecture Notes in Computer Science(), vol 10834. Springer, Cham. https://doi.org/10.1007/978-3-030-14160-8_14
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