Abstract
Isolated tissue chamber bath system and wire myograph were developed for “in vitro” investigation of vasoactive responses on isolated arteries from a variety of animal species and vascular beds. The chapter characterizes the main principles of mechanical measurement of the changes in isometric tension of vascular smooth muscles in isolated rat thoracic aorta and superior mesenteric artery and describes several protocols on how to investigate vasoactive properties of hydrogen sulfide (H2S) from the point of view of its mutual interaction with NO. Several methodological advances, results, and their interpretations in the context of the general knowledge are described. In the protocols the approach on how to study the vasoactive modulatory as well as direct action of H2S and mutual interaction of H2S with nitroso compounds, lipids, and endogenously produced NO is described.
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References
Zhang Y, Hogg N (2005) S-Nitrosothiols: cellular formation and transport. Free Radic Biol Med 38:831–838
Kimura H (2010) Hydrogen sulfide: its production, release and functions. Amino Acids 43:113–121
Ondrias K, Stasko A, Cacanyiova S et al (2008) H2S and HS-donor NaHS releases nitric oxide from nitrosothiols, metal nitrosyl complex, brain homogenate and murine L1210 leukaemia cells. Pflugers Arch 457:271–279
Bertova A, Cacanyiova S, Kristek F et al (2010) The hypothesis of the main role of H2S in coupled sulphide-nitroso signalling pathway. Gen Physiol Biophys 29:402–410
Tomaskova Z, Cacanyiova S, Benco A et al (2009) Lipids modulate H2S/HS-induced NO release from S-nitrosoglutathione. Biophys Res Commun 390:1241–1244
Berenyiova A, Grman M, Mijusjovic A et al (2015) The reaction products of sulfide and S-nitrosoglutathione are potent vasorelaxants. Nitric Oxide 46:123–130
Cortese-Krott MM, Fernandez BO, Santos JL et al (2004) Nitrosopersulfide (SSNO−) accounts for sustained NO bioactivity of S-nitrosothiols following reaction with sulfide. Redox Biol 2:234–244
Nagy P, Pálinkás Z, Nagy A et al (2014) Chemical aspects of hydrogen sulfide measurements in physiological samples. Biochim Biophys Acta 1840:876–891
Shi Q, Feng JH, Qu HB et al (2008) A proteomic study of S-nitrosylation in the rat cardiac proteins in vitro. Biol Pharm Bull 31:1536–1540
Calderone V, Martinotti E, Scatizzi R et al (1996) A modified aortic multiple-ring preparation for functional studies. J Pharmacol Toxicol Methods 35:131–138
Furchgott RF, Zawadzki JV (1980) The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature 288:373–376
Ribback S, Pavlovic D, Herbst D et al (2012) Effects of amitriptyline, fluoxetine, tranylcypromine and venlafaxine on rat vascular smooth muscle in vitro—the role of endothelium. J Physiol Pharmacol 63:119–125
Cacanyiova S, Dovinova I, Kristek F (2013) The role of oxidative stress in acetylcholine-induced relaxation of endothelium-denuded arteries. J Physiol Pharmacol 64:241–247
Cacanyiova S, Berenyiova A, Kristek F et al (2016) The adaptive role of nitric oxide and hydrogen sulphide in vasoactive responses of thoracic aorta is triggered already in young spontaneously hypertensive rats. J Physiol Pharmacol 67:501–512
Kubo S, Doe I, Kurokawa Y et al (2007) Direct inhibition of endothelial nitric oxide synthase by hydrogen sulfide: contribution to dual modulation of vascular tension. Toxicology 232:138–146
Geng B, Cui Y, Zhao J et al (2007) Hydrogen sulphide downregulates the aortic L-arginine/nitric oxide pathway in rats. Am J Physiol Regul Integr Comp Physiol 293:R1608–R1618
Schleifenbaum J, Kohn C, Voblova N et al (2010) Systemic peripheral artery relaxation by KCNQ channel openers and hydrogen sulfide. J Hypertens 28:1875–1882
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Financial support by Slovak grants VEGA 2/0103/18, APVV-15-0565, and APVV-15-0371 is gratefully acknowledged.
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Cacanyiova, S., Berenyiova, A. (2019). In Vitro Measurement of H2S-Mediated Vasoactive Responses. In: Bełtowski, J. (eds) Vascular Effects of Hydrogen Sulfide. Methods in Molecular Biology, vol 2007. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9528-8_7
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DOI: https://doi.org/10.1007/978-1-4939-9528-8_7
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