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Protective effect of GABA-enriched fermented sea tangle against ethanol-induced cytotoxicity in HepG2 Cells

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Abstract

This study was conducted to evaluate the protective effects of GABA (gamma-amino butyric acid)-enriched sea tangle juice (STJ) by Lactobacillus brevis BJ-20 fermentation against alcohol hepatotoxicity. The protective effects were determined by assessing glutathione (GSH) content levels and gamma-glutamyl transpeptidase (GGT) activity against ethanol-induced cytotoxicity in HepG2 cells. In ethanol-treated cells, GSH content decreased to 44.35% of control (ethanol-untreated cell) values; however, treatment with fermented sea tangle juice (FSTJ) at a concentration of 25 μg/mL increased GSH levels to 67.08%.These results suggest that FSTJ may prevent intracellular GSH depletion caused by ethanol consumption. Treatment with FSTJ against alcohol-injured HepG2 cells resulted in a dose-dependent decrease in GGT activity. The expression of cytochrome P450 2E1 (CYP2E1) enzyme, a major contributor to ethanol-induced oxidative stress, was also completely inhibited in FSTJ-treated cells at a concentration of 25 μg/mL. Thus, this study demonstrated that ethanolinduced cytotoxicity could be attenuated by inhibition of GSH depletion, GGT activity, and CYP2E1 expression.

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Authors and Affiliations

  1. Marine Bioprocess Co., Ltd., Busan, 619-912, Korea

    Young-Mi Kang & Bae-Jin Lee

  2. Department of Marine Life Science and Marine Life Research & Education Center, Chosun University, Gwangju, 501-759, Korea

    Zhong-Ji Qian

  3. Department of Marine Life Science and Marine Life Research & Education Center, Chosun University, Wando, 537-863, Korea

    Zhong-Ji Qian

  4. Department of Food Science and Technology, Pukyong National University, Busan, 608-737, Korea

    Young-Mog Kim

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  1. Young-Mi Kang
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  2. Zhong-Ji Qian
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  3. Bae-Jin Lee
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  4. Young-Mog Kim
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Correspondence to Young-Mog Kim.

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Kang, YM., Qian, ZJ., Lee, BJ. et al. Protective effect of GABA-enriched fermented sea tangle against ethanol-induced cytotoxicity in HepG2 Cells. Biotechnol Bioproc E 16, 966–970 (2011). https://doi.org/10.1007/s12257-011-0154-z

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  • DOI: https://doi.org/10.1007/s12257-011-0154-z

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