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Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients

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Abstract

Prostate cancer is the most common cancer in men and second in the cancer-related frequency of mortality. Matrix metalloproteinases (MMPs) are involved in tumor invasion and metastasis in various malignancies. MMP-2 and MMP-9 are capable of digesting collagen type IV. Numerous studies have demonstrated an association between increased MMP-2 and -9 expression and tumor progression in various tumors. In this study, the expression and activities of MMP-2 and -9 were assessed in serum probes and tumor tissue from core needle biopsies and radical prostatectomies of 97 patients. MMP-2 and -9 serum expression was analyzed in a subgroup of 31 patients. MMP-9 serum expression was significantly increased in tumor patients and correlated with tumor grade. In contrast, the MMP-9 tissue expression and activity revealed no significant correlations to tumor stage or grade. The MMP-2 activity, however, showed a positive correlation for MMP-2 with tumor stage. Increased activity was predominantly detected in advanced tumor stages. Immunohistochemical analysis of MMP-2 expression demonstrated a positive association with tumor grade in prostatectomy specimens. The relative expression rates in biopsies matched in 65% with those of the prostatectomies. Detection of MMP-2 in core needle biopsies seems not to be a helpful marker for diagnostic purposes.

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Acknowledgements

We thank Tina Dreher for kindly providing protein lysates for Western-blot analyses. This work was supported by Tumorzentrum HD/MA grant 781010.

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Authors and Affiliations

  1. University Hospital Mannheim, Department of Pathology, Mannheim, Germany

    Christian G. Sauer, Alexandra Kappeler, Uwe Bleyl & Rainer Grobholz

  2. University Hospital Mannheim, Department of Urology, Mannheim, Germany

    Monika Späth & Maurice S. Michel

  3. University Hospital Mannheim, I. Department of Medicine, Mannheim, Germany

    Jens J. Kaden

  4. Research Group Hormones and Signal Transduction, German Cancer Research Center (DKFZ), Heidelberg, Germany

    Doris Mayer

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  1. Christian G. Sauer
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  2. Alexandra Kappeler
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Correspondence to Rainer Grobholz.

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Sauer, C.G., Kappeler, A., Späth, M. et al. Expression and activity of matrix metalloproteinases-2 and -9 in serum, core needle biopsies and tissue specimens of prostate cancer patients. Virchows Arch 444, 518–526 (2004). https://doi.org/10.1007/s00428-004-1016-2

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  • DOI: https://doi.org/10.1007/s00428-004-1016-2

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