Lamiaa E M Deef
Damietta University, Zoology, Faculty Member
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The present work aims to investigate the differences in the structure of liver and esophagus in five species of birds in Egypt having the same food habits.The birds under investigation are: water rail, spotted crake, little crake, common... more
The present work aims to investigate the differences in the structure of liver and esophagus in five species of birds in Egypt having the same food habits.The birds under investigation are: water rail, spotted crake, little crake, common coot and common moorhen. Five birds were obtained from local hunters in Damietta, Egypt all of these birds are belonging to the same family (Rallidae)and all of them are omnivorous, but we found some difference after microscopical examination of liver and esophagus. The birds were dissected, esophagus and liver were fixed and stained for microscopic comparative study.The results revealed that there are some histological differences after microscopical examination to liver and esophagus in five species.In esophagus: Mucous glands are bigger in little crake than that in Rallus aquaticus,muscularis mucosa layer is thicker in Rallus aquaticus and common coot than that in Gallinula chloropus, inner circular in spotted crake is thinner that in Gallinula chloropus, and most thick in common coot. In Liver: Hepatocytes in common coot are polygonal in shape, have rounded nucleusand there are some sinusoids between hepatocords in liver and presence of secondary bronchial cavity in Gallinula chloropus and Rallus aquaticus.
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Genetic datasets and evolutionary information for rails are scarce, and genetic studies for them are very sparse and insufficient compared to their widespread use in different zones. Therefore, the phylogenetic lineages among five species... more
Genetic datasets and evolutionary information for rails are scarce, and genetic studies for them are very sparse and insufficient compared to their widespread use in different zones. Therefore, the phylogenetic lineages among five species of rails belonging to the family Rallidae from Egypt were estimated and evaluated using DNA barcoding, for the first time. In the present study, mitochondrial and nuclear DNA sequence data were analyzed for these rails. Based on 16S rRNA and RAG-1 sequences, phylogenetic trees were constructed using the Maximum Evolution method, revealing that there were two separate genetic clusters, one cluster for Rallus aquaticus, Crex crex, Porzana parva and Porzana pusilla and the second cluster for Porzana porzana. Crex crex and Rallus aquaticus were laid in one clade and formed a sister group. Moreover, Porzana parva and Porzana pusilla formed another sister clade. This study demonstrated the ability of 16S rRNA and RAG-1 genes to identify rails, as well as...
Order Rodentia contains approximately half of all living mammalian species. Most of the living species diversity within rodents is within five monophyletic radiations: Hystricognathi, Sciuridae, Geomyoidea, Dipodidae, and Muroidea. The... more
Order Rodentia contains approximately half of all living mammalian species. Most of the living species diversity within rodents is within five monophyletic radiations: Hystricognathi, Sciuridae, Geomyoidea, Dipodidae, and Muroidea. The samples collected across different areas of Egypt were analyzed by means of mitochondrial cytochrome b gene sequence analysis to ascertain the true taxonomic status between the studied species. For phylogenetic analysis, the cytochrome b sequences from this study were combined with previously published data. This study reveals that all species were divided into two main clades, the first for the Gerbillinae taxon and the second for the other studied genera in the molecular phylogenetic trees and demonstrates that Gerbillus species form a sister group with Dipodillus species. The phylogenetic relationships specified for the murine genera. Clustering was obtained between Mus and Rattus as well as in all Gerbillinae species. This study provides the first...
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The present work aims to investigate the differences in the structure of liver and esophagus in five species of birds in Egypt having the same food habits.The birds under investigation are: water rail, spotted crake, little crake, common... more
The present work aims to investigate the differences in the structure of liver and esophagus in five species of birds in Egypt having the same food habits.The birds under investigation are: water rail, spotted crake, little crake, common coot and common moorhen. Five birds were obtained from local hunters in Damietta, Egypt all of these birds are belonging to the same family (Rallidae)and all of them are omnivorous, but we found some difference after microscopical examination of liver and esophagus. The birds were dissected, esophagus and liver were fixed and stained for microscopic comparative study.The results revealed that there are some histological differences after microscopical examination to liver and esophagus in five species.In esophagus: Mucous glands are bigger in little crake than that in Rallus aquaticus,muscularis mucosa layer is thicker in Rallus aquaticus and common coot than that in Gallinula chloropus, inner circular in spotted crake is thinner that in Gallinula c...
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Research Interests: Biology and DNA profiling
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Holocentrinae and Myripristinae (Holocentridae) are among the most apparent species in the nocturnal reef fish community. However, there is no clear assent regarding their phylogenetic relations, which is reported in their complicated... more
Holocentrinae and Myripristinae (Holocentridae) are among the most apparent species in the nocturnal reef fish community. However, there is no clear assent regarding their phylogenetic relations, which is reported in their complicated taxonomic history. In this study, Sargocentron spinosissimum (Temminck et Schlegel, 1843) and Sargocentron tiereoides (Bleeker, 1853) were reported from the Mediterranean coast of Egypt (Damietta coast). This is the first record of these species which is greatly distributed across Indo-Pacific regions and eastern Africa showed the success of these species to migrate to the Mediterranean water with a good adaptation to the new habitats. In the presently reported study, 26 morphometric measurements were recorded and cytochrome c oxidase subunit I (COI) barcodes were recovered for a total of 20 specimens (8 from S. spinosissimum and 12 from S. tiereoides). The specimens from the Damietta coast, Egypt show character states diagnostic of S. spinosissimum: H...
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Birds have an extra ordinary respiratory system which plays an important role in keeping the body temperature constant. The abilities of birds to sustain flight and to fly in the thin air of high altitude are striking evolutionary... more
Birds have an extra ordinary respiratory system which plays an important role in keeping the body temperature constant. The abilities of birds to sustain flight and to fly in the thin air of high altitude are striking evolutionary accomplishments. The respiratory system is vital to these strenuous feats and thus most research on the form, function and adaptive significance of the avian lung has rightly on adaptations that enable rapid rates exchange. Morphologically, the avian respiratory system is separated into the lung (the gas exchanging part) and the air sacs (the non respiratory part). Lung tissues of the migratory quail and the farmed quail were subjected to standard processing for transmission electron microscopy. The results reported differences in the number and form of the air capillaries (Acs) and blood capillaries (Bcs). The blood gas-barrier (BGB) was thinner in the migratory quail than that of the farmed quail. The lung of the migratory quail is very efficient because...
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Chelon caeruleum sp. nov. is described as a new species of Chelon encountered in the Rashid coastal region of the Mediterranean Sea, Egypt. With this finding, the new species is the sixth member of the genus Chelon. Chelon caeruleum sp.... more
Chelon caeruleum sp. nov. is described as a new species of Chelon encountered in the Rashid coastal region of the Mediterranean Sea, Egypt. With this finding, the new species is the sixth member of the genus Chelon. Chelon caeruleum sp. nov. is distinguished from its congeners by the following characters: colouration of body is dark bluish grey dorsally and the sides are blue; head length 20.5 to 21.6% SL; head width 13 to 13.5% SL; pre-first dorsal fin length 50% SL; eye diameter 4.6 to 5.2% SL; postorbital length 10.7 to 11.1% SL; unbranched pyloric caeca are 2 short and 4 long; a pair of long (one longer than the other), spine-like neural postzygapophyse on the second vertebra. DNA of Chelon caeruleum sp. nov. and five mullet species (Mugil cephalus, Liza carinata, Liza ramada, Liza aurata, and Chelon labrosus) was extracted then amplified using polymerase chain reaction (PCR) and sequenced. The mtDNA cytochrome oxidase subunit I sequence analysis confirmed that Chelon caeruleum ...
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In the present work, the structure of the tongue has been described in the context of its functional phylogenetic significance in 7 species of lizards inhabiting Damietta; Acanthodactylus boskianus (Bosc’s lizard), Acanthodactylus... more
In the present work, the structure of the tongue has been described in the context of its functional phylogenetic significance in 7 species of lizards inhabiting Damietta; Acanthodactylus boskianus (Bosc’s lizard), Acanthodactylus scutellatus (Nidua lizard), Mabuya quinquetaeniata (Bean skink), Mabuya vittata (Banded skink), Chalcides ocellatus (Ocellated skink), Hemidactylus turcicus (Warty gecko) and Chamaeleo africanus (African chameleon). There is similarity in gross appearance of the tongue between species. The tongue is bifurcated. Based on regional differences in surface morphology, the dorsum of the tongue can be divided into 3 regions. Histological examination revealed that in Acanthodactylus boskianus and Acanthodactylus scutellatus the tongue structure is nearly the same. In addition, there is a great similarity in tongue structure between Mabuya quinquetaeniata and Mabuya vittata. On the other hand, tongue musculature is the same in all studied species in chameleon which belong to another sister group.
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Reptiles are beneficial organisms in different ecosystem, and any environmental modification will be a potential impact on eliminating some reptilian species. Five locations were chosen in Damietta region, they are the North Coast of New... more
Reptiles are beneficial organisms in different ecosystem, and any environmental modification will be a potential impact on eliminating some reptilian species. Five locations were chosen in Damietta region, they are the North Coast of New Damietta City, El- Debba region, Lake Manzala and two regions in Damietta City. Lizards were consused from April, 2005 to March, 2006. Seven species were confused, they were related to 4 families and 5 genera. These species are African chamaeleon, ocellated skink, warty gecko, Bean skink, Bandad skink, Boscs lizard and Nidua lizard. Boscs and Nidua lizards were widely distributed in coastal area, while other 5 species are more related to cultivated and urban areas. With the expected reclamation of the coastal area, the lizard community will be affected in future.
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Atelerix albiventris or the four-toed hedgehog is here reported for the first time from the south-eastern of Egypt (Gebel Elba). This species is greatly distributed across central and eastern Africa. The exact identification of species is... more
Atelerix albiventris or the four-toed hedgehog is here reported for the first time from the south-eastern of Egypt (Gebel Elba). This species is greatly distributed across central and eastern Africa. The exact identification of species is fundamental for an efficient assessment of changes related to the appearance of non-native species in an environment. The biometrics and molecular markers were used to confirm the identification of A. albiventris at the species level. In the current work 757 bp and 668 bp were identified as regions of the mitochondrial cytochrome b (Cyt b) and mitochondrial 12S rRNA genes, sequences of both genes were placed in the GenBank publication database (Accession numbers are KF783143 and KJ193305) and (Accession numbers are M95109, KF783175, AC175224 and AC160876) of Cyt b and
12S rRNA respectively. Furthermore, DNA barcoding is a widely used molecular-based identification system which aids in identifying species.
12S rRNA respectively. Furthermore, DNA barcoding is a widely used molecular-based identification system which aids in identifying species.
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Triglidae is a family of benthic marine fishes known as gurnards and sea robins, and are distributed throughout all the tropical and temperate seas. Electrophoresis of PCR-RFLP and SDS-proteins were used for differentiation between three... more
Triglidae is a family of benthic marine fishes known as gurnards and sea robins, and are distributed throughout all the tropical and temperate seas. Electrophoresis of PCR-RFLP and SDS-proteins were used for differentiation between three gurnards and to detect their genetic relationship. Twenty four fish samples of the grey, the red and the piper gurnards were taken as eight samples from each species. Protein bands varied from 10 to 13 bands in the studied fishes, i.e. 13 bands in the grey gurnard and 10 bands in each of the piper and the red gurnards. The red gurnard recorded the highest similarity to the piper gurnard, while the red gurnard recorded lower similarity to the grey gurnard. Two primers were used for PCR amplification of 360 bp long fragments of the mt DNA cytochrome b (Cyt b) gene and 655 bp of the mt DNA cytochrome oxidase subunit I (COI) gene. The PCR products were digested by two different restriction enzymes DdeI and HincII. Digestion by these enzymes produced highly polymorphic restriction profiles. This enables the differentiation of these fishes and showed that there is a wide intraspecific COI and Cyt b genes. The phylogenetic tree based on the sharing protein bands and PCR-RFLP fragments gave similar results and revealed the relationship between the studied gurnards.
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Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and... more
Quail is an important and interesting group of galliform birds. The Common quail (Coturnix coturnix); the Japanese quail (Coturnix japonica); the Panda quail (Coturnix japonica); the Dotted white quail (Coturnix japonica) and the Bobwhite quail (Colinus virginianus) were used in this study. PCR-RFLP and SDS-proteins were performed to reveal the genetic characterization and genetic relationship of the studied quails. Analysis of fragments generated by digestion of PCR product with restriction enzyme NlaIII recorded highly polymorphic restriction profiles. There is a wide intraspecific COI, SEMA3E and TLX genes variability among the studied quails. Protein bands varied from10 to 18 between quails with minimum number of bands were in the Dotted white quail (10 bands) and the maximum were in the Japanese quail (18 bands) as measured by SDS-polyacrylamide gel electrophoresis. The Dotted white quail revealed the lowest similarity to the Bobwhite with a coefficient of 0.18 while The similarity coefficients between the Common quail and each one of the other quails were 0.67, 0.62, 0.45 and 0.42 for the Japanese, Panda, Dotted white and the Bobwhite quails, respectively. The results indicate t hat, PCR-RFLP and protein analyses are good techniques to evaluate genetic characterization and genetic relationship of these quails.
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Chelon caeruleum sp. nov. opisana je kao nova vrsta cipla nađenog u Rashidovom obalnom području Sredozemnog mora u Egiptu. S ovim nalazom, nova vrsta je šesti član roda Chelon. Chelon caeruleum sp. nov. razlikuje se od njegovih srodnika... more
Chelon caeruleum sp. nov. opisana je kao nova vrsta cipla nađenog u Rashidovom obalnom području Sredozemnog mora u Egiptu. S ovim nalazom, nova vrsta je šesti član roda Chelon. Chelon caeruleum sp. nov. razlikuje se od njegovih srodnika sljedećim obilježjima: dorzalna boja tijela je tamnoplavo do siva a lateralne strane su plave; duljina glave je od 20,5 do 21,6% od SD (standardne duljine); širina glave 13 do 13,5% SD; duljina prva leđne peraje 50% SD; promjer oka 4.6 do 5.2% SD; postorbitalna duljina 10,7 do 11,1% SD; posjeduje 2 kratka i 4 duga nerazgranata pilorična nastavka; kralježnična neuralna postzigapofiza se nalazi na drugom kralješku. DNK Chelon caeruleum sp. nov. i ostalih pet analiziranih vrsta cipla (Mugil cephalus, Liza carinata, Liza ramada, Liza aurata i Chelon labrosus) je ekstrahirana, pojačana reakcijom lanca polimeraze (PCR) te sekvencionirana. Analiza sekvenci podjedinice I mtDNA citokrom oksidaze potvrdila je da se Chelon caeruleum sp. nov. razlikuje se od ostalih srodnika roda Chelon.
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This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I... more
This is the first study to detection the genetic relationship between Porphyrio alleni and four Rallidae species: Fulica atra, Gallinulla angulata, Gallinulla chloropus and Porphyrio porphyrio. Mitochondrial cytochrome oxidase subunit I (COI) sequences were used as an effective marker in this study. DNA of Rallidae species were extracted, amplified using polymerase chain reaction (PCR) and then sequenced. The results obtained from information based on COI sequences revealed that Gallinulla angulata and Gallinulla chloropus fall into two separate clades and they are not monophyletic. This suggests that, two moorhens could not be laid into the same genus. In addition, Porphyrio porphyrio was included in the same genus with Porphyrio alleni but they were situated in two different clades. Porphyrio alleni was more related to Gallinulla angulate, Gallinulla chloropus and Fulica atra than Porphyrio porphyrio. It was concluded that, the mitochondrial gene COI can aid in the differentiation of studied species and finding genetic relationships between them.
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Birds have an extra ordinary respiratory system which plays an important role in keeping the body temperature constant. The abilities of birds to sustain flight and to fly in the thin air of high altitude are striking evolutionary... more
Birds have an extra ordinary respiratory system which plays an important role in keeping the body temperature constant. The abilities of birds to sustain flight and to fly in the thin air of high altitude are striking evolutionary accomplishments. The respiratory system is vital to these strenuous feats and thus most research on the form, function and adaptive significance of the avian lung has rightly on adaptations that enable rapid rates exchange. Morphologically, the avian respiratory system is separated into the lung (the gas exchanging part) and the air sacs (the non respiratory part). Lung tissues of the migratory quail and the farmed quail were subjected to standard processing for transmission electron microscopy. The results reported differences in the number and form of the air capillaries (Acs) and blood capillaries (Bcs). The blood gas-barrier (BGB) was thinner in the migratory quail than that of the farmed quail.The lung of the migratory quail is very efficient because of the presence of an extremely thin blood gas barrier than that of the farmed quail. This would contribute to the remarkable to expend energy during flight, especially at high altitude by the migratory quail which flies for longer distances, and indicate that structural adaptations may occur in the avian lung in response to functional demands.
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TThe lung of birds is the most complex and efficient gas exchanger that has evolved in the air-breathing vertebrates. It affects an organism’s shape, density, mass and characters that are important to locomotion. Lung tissue of Egyptian... more
TThe lung of birds is the most complex and efficient gas exchanger that has evolved in the air-breathing vertebrates. It affects an organism’s shape, density, mass and characters that are important to locomotion. Lung tissue of Egyptian hoopoe (upupa epops major) and European hoopoe (upupa epops epops) which inhabiting Damietta governorate in Egypt was subjected to standard processing for light and ultrastructural studies. The number and shape of parabronchus was different in the two hoopoes. The infundibula was different in its form. ACs (air capillaries) and BCs (blood capillaries) was also differed. The blood gas-barrier (BGB) was thinner in European hoopoe (migratory species) than that of Egyptian hoopoe (resident species) . The current study revealed that flying for longer distances requires enormous energy. The lung of European hoopoe is very efficient because of an extremely thin blood gas barrier than Egyptian hoopoe. Also, the lung components correlated with the metabolic demands, that are in turn set by the behavior and life style of the animal.
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In the present work, the structure of the tongue has been described in the context of its functional phylogenetic significance in 7 species of lizards inhabiting Damietta; Acanthodactylus boskianus (Bosc’s lizard), Acanthodactylus... more
In the present work, the structure of the tongue has been described in the context of its functional phylogenetic significance in 7 species of lizards inhabiting Damietta; Acanthodactylus boskianus (Bosc’s lizard), Acanthodactylus scutellatus (Nidua lizard), Mabuya quinquetaeniata (Bean skink), Mabuya vittata (Banded skink), Chalcides ocellatus (Ocellated skink), Hemidactylus turcicus (Warty gecko) and Chamaeleo africanus (African chameleon). There is similarity in gross appearance of the tongue between species. The tongue is bifurcated. Based on regional differences in surface morphology, the dorsum of the tongue can be divided into 3 regions. Histological examination revealed that in Acanthodactylus boskianus and Acanthodactylus scutellatus the tongue structure is nearly the same. In addition, there is a great similarity in tongue structure between Mabuya quinquetaeniata and Mabuya vittata. On the other hand, tongue musculature is the same in all studied species in chameleon which belong to another sister group.
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Reptiles are beneficial organisms in different ecosystem, and any environmental modification will be a potential impact on eliminating some reptilian species. Five locations were chosen in Damietta region, they are the North Coast of New... more
Reptiles are beneficial organisms in different ecosystem, and any
environmental modification will be a potential impact on eliminating some
reptilian species. Five locations were chosen in Damietta region, they are the
North Coast of New Damietta City, El- Debba region, Lake Manzala and
two regions in Damietta City. Lizards were consused from April, 2005 to
March, 2006. Seven species were confused, they were related to 4 families
and 5 genera. These species are African chamaeleon, ocellated skink, warty
gecko, Bean skink, Bandad skink, Boscs lizard and Nidua lizard. Boscs and
Nidua lizards were widely distributed in coastal area, while other 5 species
are more related to cultivated and urban areas. With the expected
reclamation of the coastal area, the lizard community will be affected in
future.
environmental modification will be a potential impact on eliminating some
reptilian species. Five locations were chosen in Damietta region, they are the
North Coast of New Damietta City, El- Debba region, Lake Manzala and
two regions in Damietta City. Lizards were consused from April, 2005 to
March, 2006. Seven species were confused, they were related to 4 families
and 5 genera. These species are African chamaeleon, ocellated skink, warty
gecko, Bean skink, Bandad skink, Boscs lizard and Nidua lizard. Boscs and
Nidua lizards were widely distributed in coastal area, while other 5 species
are more related to cultivated and urban areas. With the expected
reclamation of the coastal area, the lizard community will be affected in
future.
Research Interests:
The structure of the lizard community in Damietta was analyzed in the present study. Five locations were selected as a study area, each represents different habitat of Damietta Governorate. Seven lizard species were recorded which are... more
The structure of the lizard community in
Damietta was analyzed in the present study.
Five locations were selected as a study area,
each represents different habitat of Damietta
Governorate. Seven lizard species were
recorded which are related to four families
and five genera. They were Chamaeleo
africanus, Hemidactylus turcicus, Chalcides
ocellatus, Mabuya quinquetaeniata, Mabuya
vittata, Acanthodactylus boskianus and
Acanthodactylus scutellatus. The lacertid
lizards Acanthodactylus boskianus and
Acanthodactylus scutellatus were the most
common species in the community. Therefore,
the study was extended to reveal the genetic
variability for the population of these two
species in Damietta. Four arbitrary chosen
enzymes were analyzed electrophoretically.
These enzymes were Glucose-6-phosphate
dehydrogenase (G6PDH), esterase (EST),
lactate dehydrogenase (LDH) and acid
phosphatase (ACP). Eleven presumed gene
loci have been recorded. A. scutellatus
showed higher genetic variability with a
polymorphism of 90% of its population
compared to 82% for the samples of A.
boskianus.
Damietta was analyzed in the present study.
Five locations were selected as a study area,
each represents different habitat of Damietta
Governorate. Seven lizard species were
recorded which are related to four families
and five genera. They were Chamaeleo
africanus, Hemidactylus turcicus, Chalcides
ocellatus, Mabuya quinquetaeniata, Mabuya
vittata, Acanthodactylus boskianus and
Acanthodactylus scutellatus. The lacertid
lizards Acanthodactylus boskianus and
Acanthodactylus scutellatus were the most
common species in the community. Therefore,
the study was extended to reveal the genetic
variability for the population of these two
species in Damietta. Four arbitrary chosen
enzymes were analyzed electrophoretically.
These enzymes were Glucose-6-phosphate
dehydrogenase (G6PDH), esterase (EST),
lactate dehydrogenase (LDH) and acid
phosphatase (ACP). Eleven presumed gene
loci have been recorded. A. scutellatus
showed higher genetic variability with a
polymorphism of 90% of its population
compared to 82% for the samples of A.
boskianus.