Czech Journal of Animal Science, 2018 (vol. 63), issue 3
Genetic diversity and admixture in three native draught horse breeds assessed using microsatellite markersOriginal Paper
Hana Vostrá-Vydrová, Luboš Vostrý, Barbora Hofmanová, Nina Moravčíková, Zdeňka Veselá, Irena Vrtková, Alexandra Novotná, Radovan Kasarda
Czech J. Anim. Sci., 2018, 63(3):85-93 | DOI: 10.17221/51/2017-CJAS 
In this study, we aimed to estimate and compare genetic diversity of two native draught horse breeds and check the possible influence of Noriker breed population on these native breeds. Genetic analyses of relationships and admixture were performed in two native endangered draught horse populations (Silesian Noriker and Czech-Moravian Belgian horses) and one open breed (Noriker). Totally 104 alleles from 13 microsatellite loci were detected in 1298 horses. The average number of alleles per locus was the highest in the Czech-Moravian Belgian horse (7.62) and the lowest in the Silesian Noriker (7.31), the differences were non-significant, whereas the...
Protection of bovine mammary epithelial cells from hydrogen peroxide-induced oxidative cell damage by seleniumOriginal Paper
Yong Mei Guo, Jian Gong, Ya Guang Zheng, Bin Lin Shi, Xiao Yu Guo, Su Mei Yan
Czech J. Anim. Sci., 2018, 63(3):94-102 | DOI: 10.17221/76/2017-CJAS
The uncontrolled release of arachidonic acid (ARA) and its metabolism by lipoxygenase (LOX) pathway can induce and aggravate cellular oxidative stress. Selenium (Se) is an integral part of some antioxidative selenoproteins and may protect cells from oxidative damage by modulating ARA release and metabolism. The present study aimed to investigate the protective response of Se against hydrogen peroxide (H2O2)-induced oxidative damage in bovine mammary epithelial cells (BMECs). The BMECs were incubated for 24 h in serum-free medium and then divided into four groups randomly. The cells in groups 1 and 2 were subsequently incubated...
Gender and age effects on the expression of genes related to lipid metabolism in broiler's liverOriginal Paper
Angélica de Souza Khatlab, Ana Paula Del Vesco, Eliane Gasparino, Adhemar Rodrigues de Oliveira Neto
Czech J. Anim. Sci., 2018, 63(3):103-109 | DOI: 10.17221/41/2017-CJAS
Two experiments were conducted to assess gender (Experiment 1) and age (Experiment 2) effects on the expression of genes related to lipid metabolism in broiler chickens. The expression of fatty acid synthase (FAS), apolipoprotein A-I (APOA-I), apolipoprotein B (APOB), adiponectin (ADIPOQ), liver kinase B1 (LKB1), and AMP-activated protein kinase α-1 (AMPKα-1) genes was evaluated by qRT-PCR. In Experiment 1, we observed a gender effect on feed intake, as male broilers presented greater feed intake than females. Female broilers presented greater gene expression of FAS,...
Two mitochondrial genes are associated with performance traits in farmed raccoon dogs (Nyctereutes procyonoides)Original Paper
Sylwia Nisztuk-Pacek, Brygida Slaska, Grzegorz Zieba, Iwona Rozempolska-Rucinska
Czech J. Anim. Sci., 2018, 63(3):110-118 | DOI: 10.17221/2/2017-CJAS
The relationships between chosen mitochondrial genes polymorphisms and performance traits in raccoon dogs were determined. The study involved 354 farmed raccoon dogs. Blood collected from the animals was the analysed biological material. Mitochondrial DNA genes, i.e. MT-CO1 (mitochondrially encoded cytochrome c oxidase I), MT-CO2 (mitochondrially encoded cytochrome c oxidase II), and MT-CYB (mitochondrially encoded cytochrome b) were amplified using the polymerase chain reaction method. The amplicons obtained were sequenced and subjected to bioinformatics analysis. Based on the nucleotide sequences, three haplotypes for the...
Determination of in vitro antibacterial activity of plant oils containing medium-chain fatty acids against Gram-positive pathogenic and gut commensal bacteriaOriginal Paper
Petra Hovorková, Klára Laloučková, Eva Skřivanová
Czech J. Anim. Sci., 2018, 63(3):119-125 | DOI: 10.17221/70/2017-CJAS
Increasing antibiotic resistance has led to a ban on antibiotic use in feed additives in the EU. Therefore, new non-antibiotic, pathogen-inhibiting agents are urgently needed. Inhibitory effects of eight plant oils containing medium-chain fatty acids (MCFAs) were evaluated against Gram-positive pathogenic and beneficial bacteria. The oils tested were palm, red palm, palm kernel (Elaeis guineensis), coconut (Cocos nucifera), babassu (Attalea speciosa), murumuru (Astrocaryum murumuru), tucuma (Astrocaryum vulgare), and Cuphea oil (Cuphea ignea); the method used was broth microdilution, and...