Czech Journal of Animal Science, 2017 (vol. 62), issue 2
Repeats as global DNA methylation marker in bovine preimplantation embryosOriginal Paper
Wenwen Li, Ann Van Soom, Luc Peelman
Czech J. Anim. Sci., 2017, 62(2):43-50 | DOI: 10.17221/29/2016-CJAS 
DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a bisulfite sequencing based method aimed at repetitive sequences including three retrotransposons (L1_BT, BovB, and ERV1-1-I_BT) and Satellite I. A more accurate estimate of the global DNA methylation level compared to previous methods using only one repeat sequence, like Alu, could be made by calculation of the weighted arithmetic mean of multiple repetitive sequences, considering the copy number of each repetitive...
Effect of "Wooden Breast" appearance on poultry meat quality, histological traits, and lesions characterizationOriginal Paper
Antonella Dalle Zotte, Giulia Tasoniero, Eero Puolanne, Hervé Remignon, Mattia Cecchinato, Elena Catelli, Marco Cullere
Czech J. Anim. Sci., 2017, 62(2):51-57 | DOI: 10.17221/54/2016-CJAS
The purposes of the study were to investigate the effects of Wooden Breast (WB) myodegeneration on poultry meat quality and to give a contribution in typing lesions morphology. At a poultry meat cutting facility, 474 carcasses of a high-breast-yield hybrid chickens were inspected for WB condition, and 30 normal (N) and 30 affected (WB) breast fillets (Pectoralis major) were randomly selected. The WB condition represented 53.2% of the examined carcasses. Weight, cross sectional area (CSA), pH, L*, a*, b* colour values, water-holding capacity, and Warner-Bratzler shear force were determined. Samples were also visually and histologically evaluated....
Dietary chitosan affects metabolism of arachidonic acid in weaned pigletsOriginal Paper
Jun-liang Li, Yuan-qing Xu, Bin-lin Shi, Deng-sheng Sun, Su-mei Yan, Xiao-yu Guo
Czech J. Anim. Sci., 2017, 62(2):58-66 | DOI: 10.17221/39/2016-CJAS
The effects of chitosan on immune function via arachidonic acid (AA) pathway in weaned piglets were investigated. A total of 180 piglets (Duroc × Yorkshire × Landrace) were randomly assigned to 5 dietary treatments and fed a basal diet supplemented with 0 (control), 100, 500, 1000, and 2000 mg chitosan/kg feed, respectively. Results showed that serum AA, prostaglandin E2 (PGE2), and leukotriene B4 (LTB4) contents in piglets were increased in a linear or quadratic dose-dependent manner with increasing chitosan on day 28 (P < 0.05). Chitosan increased serum cytosolic-phospholipase A2 (cPLA2) activity in a linear or quadratic dose-dependent...
Development and application of LC/HRPS for quantification of adenine nucleotides, creatine phosphate, and creatine in sturgeon spermatozoaOriginal Paper
Pavlo Fedorov, Roman Grabic, Ganna Fedorova, Jacky Cosson, Sergii Boryshpolets, Borys Dzyuba
Czech J. Anim. Sci., 2017, 62(2):67-74 | DOI: 10.17221/4/2016-CJAS
The objective of this study was to investigate the applicability of liquid chromatography coupled with high resolution product scan (LC/HRPS) mass spectrometry for simultaneous quantification of adenine nucleotides, creatine phosphate, and creatine levels after fixation of sturgeon sperm by perchloric acid. This approach has been used for the determination of adenine nucleotides, creatine, and creatine phosphate in biological tissues, but no applications to sperm cells have been reported to date. The results of method validation showed that specific correction factors for the calculation of macroergic phosphate content in sperm cells extracted by perchloric...
The influence of milking on the teat canal of dairy cows determined by ultrasonographic measurementsOriginal Paper
Peter Strapák, Eva Strapáková, Mária Rušinová, Iveta Szencziová
Czech J. Anim. Sci., 2017, 62(2):75-81 | DOI: 10.17221/68/2015-CJAS
The effect of milking on the length and diameter of the teat canal during teat regeneration immediately after milking was evaluated. The practical measurements of the teat canal were performed on 70 Holstein cows using ultrasound Aloka Prosound device 2. A special "bath method" of the teat ultrasonographic scanning was used at set time intervals - before milking, immediately after milking, and 30, 60, 90, and 120 min after milking. Before milking, the average length of the teat canal was 10.67 mm and 9.3 mm and the teat canal diameter was 1.11 mm and 1.09 mm for front and rear teats, respectively. The average length of the front and rear teat canal...
Dynamic expression of HSP90B1 mRNA in the hypothalamus of two Chinese chicken breeds under heat stress and association analysis with a SNP in Huainan chickensOriginal Paper
Yi Wan, Chendong Ma, Peipei Wei, Qi Fang, Xing Guo, Bangyuan Zhou, Runshen Jiang
Czech J. Anim. Sci., 2017, 62(2):82-87 | DOI: 10.17221/8/2016-CJAS
The effects of heat stress on HSP90B1 messenger RNA (mRNA) expression in the hypothalamus of chicken were investigated and HSP90B1 variations were detected. Females of two Chinese chicken breeds (Huainan and Wenchang) were used for the experiments. At 64 days of age, the ambient temperature (24 ± 1°C) was increased to 35 ± 1°C for 24 h (heat stress), then decreased to 24 ± 1°C for 24 h (recovery). Hypothalamus samples were collected at 0, 12, and 24 h during heat stress, as well as 12 and 24 h during recovery. The HSP90B1 mRNA expression increased significantly during heat stress and significantly decreased...