Czech Journal of Animal Science, 2014 (vol. 59), issue 8
Effects of laying hens housing system on laying performance, egg quality characteristics, and egg microbial contaminationOriginal Paper
M. Englmaierová, E. Tůmová, V. Charvátová, M. Skřivan
Czech J. Anim. Sci., 2014, 59(8):345-352 | DOI: 10.17221/7585-CJAS 
The objective of this study was to compare the performance, egg quality, and microbial contamination of egg shells from hens maintained in different housing systems, such as conventional and enriched cages, litter, and aviaries. The housing system significantly (P < 0.001) influenced the performance characteristics. The highest egg production, lowest daily feed consumption, and feed conversion ratio were measured in conventional cages compared to litter and aviaries. Higher egg shell and albumen qualities were observed in conventional cages, whereas hens housed in enriched cages and aviaries laid eggs with a higher yolk index (P <...
Effects of the replacement of corn oil with linseed oil on fatty acid composition and the expression of lipogenic genes in broiler chickensOriginal Paper
W. Chen, R. Zhao, B.X. Yan, J.S. Zhang, Y.Q. Huang, Z.X. Wang, Y.M. Guo
Czech J. Anim. Sci., 2014, 59(8):353-364 | DOI: 10.17221/7586-CJAS
The effect of dietary 18:2n-6/18:3n-3 ratio (by the replacement of corn oil with linseed oil) on n-3 polyunsaturated fatty acids (PUFA) enrichment in breast muscle of broiler chickens and the expression of lipogenic genes were investigated. Broiler chickens were fed ad libitum with diets containing 5% corn oil (CO), 3.75% corn oil + 1.25% linseed oil (CL1), 2.5% corn oil + 2.5% linseed oil (CL2), and 5% linseed oil (LO) based on the basic diets, respectively. Dietary 18:2n-6/18:3n-3 ratio did not affect 42-day body weight and 0-42-day feed conversion efficiency (feed/gain, P > 0.05) of broiler chickens, however, 5% linseed oil...
Effect of DGAT1 polymorphisms on the estimated breeding values of Czech Simmental siresOriginal Paper
L. Hanusová, A. Míková, L. Večerek, D. Schroeffelová, V. Řehout, L. Tothová, K. Vernerová, B. Hosnedlová, J. Čítek
Czech J. Anim. Sci., 2014, 59(8):365-373 | DOI: 10.17221/7587-CJAS
The aim was to evaluate the effect of polymorphisms in the promoter and in the coding region of the DGAT1 gene on the estimated breeding values (EBV) of Czech Simmental sires. The K232A polymorphism (n = 191) in the coding region was genotyped by PCR/RFLP, and the KU and SA polymorphisms in the promoter (n = 203) were identified in an automatic sequencer. In the K232A polymorphism, the frequency of the genotype AA (Alanine) was greater than that of the genotype KA, the homozygous genotype KK (Lysine) was not found. Similarly, the allele A predominated...
Susceptibility of Escherichia coli and Clostridium perfringens to sucrose monoesters of capric and lauric acidOriginal Paper
E. Skřivanová, Š. Pražáková, O. Benada, P. Hovorková, M. Marounek
Czech J. Anim. Sci., 2014, 59(8):374-380 | DOI: 10.17221/7588-CJAS
The sucrose monoesters of capric and lauric acid were tested for their antibacterial activity towards two foodborne enteropathogenic bacteria - Escherichia coli (CCM 3954 - serotype O6 and E22 - serotype O103) and Clostridium perfringens (CNCTC 5459 and CIP 105178). Antibacterial activity was evaluated by the plating technique. Sucrose monocaprate significantly decreased the number of viable cells of E. coli at all tested concentrations (0.1-5 mg/ml). The overnight incubation of C. perfringens with the sucrose ester of lauric acid at 0.1-5 mg/ml reduced the number of viable cells below the detection limit (2 log10...
Isolation and cryopreservation of early stages of germ cells of tench (Tinca tinca)Original Paper
Z. Linhartová, M. Rodina, H. Guralp, I. Gazo, T. Saito, M. Pšenička
Czech J. Anim. Sci., 2014, 59(8):381-390 | DOI: 10.17221/7589-CJAS
A practical technique for isolation and cryopreservation of tench (Tinca tinca) (Cyprinidae, Teleostei) early stages of germ cells (GC), including spermatogonia and spermatocytes, is reported for the first time. The germ-line cells possess the ability to differentiate into functional gametes of both sexes. These early stages of germ cells are small enough to be well-suited to cryopreservation, which, together with their high level of plasticity, makes their preservation a promising tool for maintaining genetic resources. Testicular cells were distinguished and separated by Percoll gradient, with the highest proportion of GC (62.2%) obtained...