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To determine temporal trends in HIV infection and risk factors among persons seeking anonymous HIV testing in Santos, Brazil. Data and sera from persons testing for HIV from 1996 to 1999 were used. Exposures were abstracted from HIV... more
To determine temporal trends in HIV infection and risk factors among persons seeking anonymous HIV testing in Santos, Brazil. Data and sera from persons testing for HIV from 1996 to 1999 were used. Exposures were abstracted from HIV testing risk assessments. Stored HIV-positive sera were tested to identify recently acquired HIV infection using a serologic testing algorithm for detecting recent HIV seroconversion (STARHS). Independent associations between exposures and recently acquired HIV infection were determined using multivariate analyses. Overall, estimated HIV incidence was 2.0% (95% CI: 1.1-3.5) for the 4-year period: 1.2% (95% CI: 0.5-2.6) in women and 2.7% (95% CI: 1.3-5.0) in men. Incidence increased among women but remained stable among men. Exposures independently associated with incident infection included a history of sex work (OR= 5.4, 95% CI: 1.5-18.7), concurrent syphilis infection (OR =4.1, 95% CI: 1.4-11.9), anal sex (OR = 3.0, 95% CI: 1.3-7.1), and having an HIV-positive sexual partner (OR= 1.4, 95% CI: 1.1-1.9). This study further demonstrates the public health utility of using the STARHS for the assessment of emerging trends in the HIV epidemic. Results from this study will help to target appropriate prevention strategies directed toward at-risk populations in Santos.
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Since flow cytometry allows rapid,simultaneous and quantitative measurementsrelated to cell morphology andphysiologicy, the lipid-specificfluorescent dye, Nile Red, was employed forthe in vivo lipid quantification of Crypthecodinium... more
Since flow cytometry allows rapid,simultaneous and quantitative measurementsrelated to cell morphology andphysiologicy, the lipid-specificfluorescent dye, Nile Red, was employed forthe in vivo lipid quantification of Crypthecodinium cohnii, a heterotrophicmarine dinoflagellate rich inpolyunsaturated long chain fatty acids. Thefluorescence signal was linearly correlatedwith the neutral and polar lipid content asdetermined by gravimetric techniques. Asignificant correlation of NR signal wasalso observed between the polar to neutrallipid ratio and docohexaenoic acid percell. The results demonstrate a method forrapid lipid quantification that can be usedin the selection, isolation and culturecontrol of C. cohnii clones with highlipid and DHA content.