... The authors are indebted to Drs AG Ogston and R. Cecil for their criticism of the manuscript.... more ... The authors are indebted to Drs AG Ogston and R. Cecil for their criticism of the manuscript. REFERENCES Beaven, GH, Ellis, M. & White, JC (1956). Nature, Lond., 178, 857. ... of Amberlite IR-120 respectively. The columns are mounted at succes-sively lower levels in a rack. ...
Treatment of female mice with testosterone propionate led to a pronounced, but gradual increase i... more Treatment of female mice with testosterone propionate led to a pronounced, but gradual increase in kidney beta-adrenergic receptor complement. The specific binding of [125I]iodohydroxybenzylpindolol rose 2-3-fold above the control levels after 8-12 days of the treatment. No significant changes were detected prior to the fourth day of androgen administration. No gross changes in either the binding strength or cooperativity of the binding were apparent in membrane preparations from treated animals. Averages of the high-affinity binding constant estimates were 1.3 +/- 0.3 nmol in controls, vs. 1.6 +/- 0.5 nmol in treated animals (15 groups each) in competition with pindolol, with the Hill slope factors of 0.98 +/- 0.08 for controls, and 0.91 +/- 0.07 for the treated animal membrane preparations. Scatchard estimates of the binding constants in self-competed [125I]iodohydroxybenzylpindolol binding were about 160 pmol in both control and treated animals. Competition experiments using isop...
Crude preparations of biologically active mRNA, which code for a myeloma (MPC-11) light chain, we... more Crude preparations of biologically active mRNA, which code for a myeloma (MPC-11) light chain, were isolated by two successive sucrose gradient centrifugations of RNA extracted from membrane-bound ribosomes, mRNA thus obtained was separated into a poly(A)-rich and a poly(A)-poor fraction by oligo(dT)-cellulose chromatography. Both these fractions were able to direct the synthesis of light chains in reconstituted cell-free systems derived from heterologous cells (ascites tumor lysates) and homologous cells (MPC-11 cells grown in suspension culture). The identity of the products in vitro was confirmed by comparing their migration with that of light chains produced in vivo upon electrophoresis in sodium dodecylsulphate/polyacrylamide gels, and from the profiles of tryptic peptides obtained by chromatography on Aminex A-5 ion-exchange columns. Template activity of the poly(A)-rich light chain mRNA fraction showed very little variation during the cell cycle. The activity of the poly(A)-poor fraction on the other hand was maximal during the early S phase. It is concluded that maximal synthesis of immunoglobulins observed in vivo during the late G1 phase of the cell cycle is achieved by translational control mechanisms.
Summary The administration of hexahydrocoenzyme Q 4 (20 μg/g of body weight) resulted in an incre... more Summary The administration of hexahydrocoenzyme Q 4 (20 μg/g of body weight) resulted in an increase in muscle strength of dystrophic mice with a hanging time on a vertical screen of 44.5 sec after 4 weeks of treatment compared to 5.5 sec for untreated controls. A slight ...
... The authors are indebted to Drs AG Ogston and R. Cecil for their criticism of the manuscript.... more ... The authors are indebted to Drs AG Ogston and R. Cecil for their criticism of the manuscript. REFERENCES Beaven, GH, Ellis, M. & White, JC (1956). Nature, Lond., 178, 857. ... of Amberlite IR-120 respectively. The columns are mounted at succes-sively lower levels in a rack. ...
Treatment of female mice with testosterone propionate led to a pronounced, but gradual increase i... more Treatment of female mice with testosterone propionate led to a pronounced, but gradual increase in kidney beta-adrenergic receptor complement. The specific binding of [125I]iodohydroxybenzylpindolol rose 2-3-fold above the control levels after 8-12 days of the treatment. No significant changes were detected prior to the fourth day of androgen administration. No gross changes in either the binding strength or cooperativity of the binding were apparent in membrane preparations from treated animals. Averages of the high-affinity binding constant estimates were 1.3 +/- 0.3 nmol in controls, vs. 1.6 +/- 0.5 nmol in treated animals (15 groups each) in competition with pindolol, with the Hill slope factors of 0.98 +/- 0.08 for controls, and 0.91 +/- 0.07 for the treated animal membrane preparations. Scatchard estimates of the binding constants in self-competed [125I]iodohydroxybenzylpindolol binding were about 160 pmol in both control and treated animals. Competition experiments using isop...
Crude preparations of biologically active mRNA, which code for a myeloma (MPC-11) light chain, we... more Crude preparations of biologically active mRNA, which code for a myeloma (MPC-11) light chain, were isolated by two successive sucrose gradient centrifugations of RNA extracted from membrane-bound ribosomes, mRNA thus obtained was separated into a poly(A)-rich and a poly(A)-poor fraction by oligo(dT)-cellulose chromatography. Both these fractions were able to direct the synthesis of light chains in reconstituted cell-free systems derived from heterologous cells (ascites tumor lysates) and homologous cells (MPC-11 cells grown in suspension culture). The identity of the products in vitro was confirmed by comparing their migration with that of light chains produced in vivo upon electrophoresis in sodium dodecylsulphate/polyacrylamide gels, and from the profiles of tryptic peptides obtained by chromatography on Aminex A-5 ion-exchange columns. Template activity of the poly(A)-rich light chain mRNA fraction showed very little variation during the cell cycle. The activity of the poly(A)-poor fraction on the other hand was maximal during the early S phase. It is concluded that maximal synthesis of immunoglobulins observed in vivo during the late G1 phase of the cell cycle is achieved by translational control mechanisms.
Summary The administration of hexahydrocoenzyme Q 4 (20 μg/g of body weight) resulted in an incre... more Summary The administration of hexahydrocoenzyme Q 4 (20 μg/g of body weight) resulted in an increase in muscle strength of dystrophic mice with a hanging time on a vertical screen of 44.5 sec after 4 weeks of treatment compared to 5.5 sec for untreated controls. A slight ...
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