In the present investigation, we described the green synthesis of silver nanoparticles using plan... more In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 mm) against isolated bacteria S. sonnei at 40 μg/ml.
ABSTRACT Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the e... more ABSTRACT Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the effect of different growth regulators on the in vitro direct shoot and root initiation methods. Four different explants were used to establish callus shoot and root direct regeneration. In the first experiment leaf segments were cultured on MS basal supplemented with 2,4 – Dichlorophenoxyacetic acid (2,4 – D, 0.1-20.0 mg/L), with combination of Naphthalene acetic acid (NAA 0.1-20 mg/L) and Benzylaminopurin (0.1-20 mg/L). This new protocol was standardized for easy mass propagation of W. somnifera medicinal plant. Callus initiation was observed best in MS media with (2,4- D 1.0-5.0 mg/L) after 16-20 days (93%). Highest maximum number of multiple shoots was obtained on MS medium (BAP 3.0 – 5.0 mg/L). The shoots were seaperated from the multiple-shoots, transferred to MS medium supplemented with 1.5 – 20 mg/L NAA favored roots formation occurred in most of the shoot let 88% were successfully achieved in the MS media. The rooted plantlets were transferred to polythene bags which was containing vermi compost, sand and red soil in the ratio of 1:2:2 and kept in a mist house. After acclimatization in the mist house for 2-months, it transferred to greenhouse. The plantlets were successfully planted in the field.
CeO2 nanoparticles (NPs) were green synthesized using Gloriosa superba L. leaf extract. The synth... more CeO2 nanoparticles (NPs) were green synthesized using Gloriosa superba L. leaf extract. The synthesized nanopar- ticles retained the cubic structure, which was confirmed by X-ray diffraction studies. The oxidation states of the elements (C (1s), O (1s) and Ce (3d)) were confirmed by XPS studies. TEM images showed that the NPs possessed spherical shape and particle size of 5 nm. The Ce–O stretching bands were observed at 451 cm−1 and 457 cm−1 from the FT-IR and Raman spectra respectively. The band gap of the CeO2 NPs was estimated as 3.78 eV from the UV–visible spectrum. From the photoluminescence measurements, the broad emission composed of eight dif- ferent bands were found. The antibacterial studies performed against a set of bacterial strains showed that Gram positive (G+) bacteria were relatively more susceptible to the NPs than Gram negative (G−) bacteria. The toxicological behavior of CeO2 NPs was found due to the synthesized NPs with uneven ridges and oxygen defects in CeO2 NPs.
In the present investigation, we described the green synthesis of silver nanoparticles using plan... more In the present investigation, we described the green synthesis of silver nanoparticles using plant leaf extract of Hemidesmus indicus. The synthesized silver nanoparticles were characterized by UV-visible spectroscopy, fourier transform infra-red spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX). TEM images proved that the synthesized silver nanoparticles were spherical in shape with an average particle size of 25.24 nm. To evaluate antibacterial efficacy, bacteria was isolated from poultry gut and subjected to 16S rRNA characterization and confirmed as Shigella sonnei. The in vitro antibacterial efficacy of synthesized silver nanoparticles was studied by agar bioassay, well diffusion and confocal laser scanning microscopy (CLSM) assay. The H. indicus mediated synthesis of silver nanoparticles shows rapid synthesis and higher inhibitory activity (34 mm) against isolated bacteria S. sonnei at 40 μg/ml.
ABSTRACT Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the e... more ABSTRACT Leaf and Cotyledon explants of Withania somnifera (L). Dunal were used to evaluate the effect of different growth regulators on the in vitro direct shoot and root initiation methods. Four different explants were used to establish callus shoot and root direct regeneration. In the first experiment leaf segments were cultured on MS basal supplemented with 2,4 – Dichlorophenoxyacetic acid (2,4 – D, 0.1-20.0 mg/L), with combination of Naphthalene acetic acid (NAA 0.1-20 mg/L) and Benzylaminopurin (0.1-20 mg/L). This new protocol was standardized for easy mass propagation of W. somnifera medicinal plant. Callus initiation was observed best in MS media with (2,4- D 1.0-5.0 mg/L) after 16-20 days (93%). Highest maximum number of multiple shoots was obtained on MS medium (BAP 3.0 – 5.0 mg/L). The shoots were seaperated from the multiple-shoots, transferred to MS medium supplemented with 1.5 – 20 mg/L NAA favored roots formation occurred in most of the shoot let 88% were successfully achieved in the MS media. The rooted plantlets were transferred to polythene bags which was containing vermi compost, sand and red soil in the ratio of 1:2:2 and kept in a mist house. After acclimatization in the mist house for 2-months, it transferred to greenhouse. The plantlets were successfully planted in the field.
CeO2 nanoparticles (NPs) were green synthesized using Gloriosa superba L. leaf extract. The synth... more CeO2 nanoparticles (NPs) were green synthesized using Gloriosa superba L. leaf extract. The synthesized nanopar- ticles retained the cubic structure, which was confirmed by X-ray diffraction studies. The oxidation states of the elements (C (1s), O (1s) and Ce (3d)) were confirmed by XPS studies. TEM images showed that the NPs possessed spherical shape and particle size of 5 nm. The Ce–O stretching bands were observed at 451 cm−1 and 457 cm−1 from the FT-IR and Raman spectra respectively. The band gap of the CeO2 NPs was estimated as 3.78 eV from the UV–visible spectrum. From the photoluminescence measurements, the broad emission composed of eight dif- ferent bands were found. The antibacterial studies performed against a set of bacterial strains showed that Gram positive (G+) bacteria were relatively more susceptible to the NPs than Gram negative (G−) bacteria. The toxicological behavior of CeO2 NPs was found due to the synthesized NPs with uneven ridges and oxygen defects in CeO2 NPs.
Uploads
Papers by A. Arumugam
ticles retained the cubic structure, which was confirmed by X-ray diffraction studies. The oxidation states of the
elements (C (1s), O (1s) and Ce (3d)) were confirmed by XPS studies. TEM images showed that the NPs possessed
spherical shape and particle size of 5 nm. The Ce–O stretching bands were observed at 451 cm−1 and 457 cm−1
from the FT-IR and Raman spectra respectively. The band gap of the CeO2 NPs was estimated as 3.78 eV from the
UV–visible spectrum. From the photoluminescence measurements, the broad emission composed of eight dif-
ferent bands were found. The antibacterial studies performed against a set of bacterial strains showed that
Gram positive (G+) bacteria were relatively more susceptible to the NPs than Gram negative (G−) bacteria.
The toxicological behavior of CeO2 NPs was found due to the synthesized NPs with uneven ridges and oxygen
defects in CeO2 NPs.
ticles retained the cubic structure, which was confirmed by X-ray diffraction studies. The oxidation states of the
elements (C (1s), O (1s) and Ce (3d)) were confirmed by XPS studies. TEM images showed that the NPs possessed
spherical shape and particle size of 5 nm. The Ce–O stretching bands were observed at 451 cm−1 and 457 cm−1
from the FT-IR and Raman spectra respectively. The band gap of the CeO2 NPs was estimated as 3.78 eV from the
UV–visible spectrum. From the photoluminescence measurements, the broad emission composed of eight dif-
ferent bands were found. The antibacterial studies performed against a set of bacterial strains showed that
Gram positive (G+) bacteria were relatively more susceptible to the NPs than Gram negative (G−) bacteria.
The toxicological behavior of CeO2 NPs was found due to the synthesized NPs with uneven ridges and oxygen
defects in CeO2 NPs.