Ferroptosis is a non-apoptotic type of cell death that is induced by uncontrolled iron-dependent phospholipid peroxidation and has important roles in disease. This Expert Recommendation article summarizes ferroptosis regulation and mechanisms and provides recommendations to increase the reproducibility and robustness of ferroptosis research.

Membrane contact sites between organelles facilitate the exchange of ions, lipids and metabolites. This Expert Recommendation highlights recent advances in defining the organelle contactome and discusses challenges and future directions in membrane contact site research.

In this Tools of the Trade article, Ding (Wang lab) describes the development of tissue expansion-enhanced mass-spectrometry imaging (TEMI), which combines an optimized tissue expansion method with mass spectrometry imaging to obtain multi-omic data at high spatial resolution and is compatible with expansion microscopy.

Timo Kohler highlights a study in which in vitro fertilization and cell culture techniques were adapted to generate chimeric blastocysts from northern and southern white rhinoceros species and establish embryonic stem cell lines — which has important implications for the conservation of endangered species and biodiversity.

In this Tools of the Trade article, Zhang (Xiao Lab) highlights the development of a rotor-based fluorescent amino acid that acts as a sensor for crowded protein microenvironments, enabling researchers to monitor protein behaviour in vivo.

Barbara Demmig-Adams discusses a 1998 study that demonstrated in plants that carotenoids can remove dangerous excess energy from chlorophyll, and the implications of this work, including for research aimed at improving plant stress resilience.

A study finds that mechanical activation leads to microtubule rearrangement, facilitating the proteasomal degradation of an inhibitor of YAP/TAZ, thus promoting YAP/TAZ nuclear translocation.

A study showing that human cells encode a functional cyclin-dependent kinase, thereby demonstrating that cell-cycle regulation is conserved across eukaryotes.