Background: Laser photobiomodulation has been used in the treatment of various injuries and disea... more Background: Laser photobiomodulation has been used in the treatment of various injuries and diseases. This promotes modulation of the inflammatory process, edema reduction and devitalized tissue regeneration. The advantages of Antimicrobial photodynamic therapy are its easy application and the absence of side effects. Other advantages are the cost of the therapy, minimal damage to animal tissue, the broad spectrum of action, and efficiency against strains resistant to antimicrobials. The aim of this study was to report the clinical and their resolution in a female dog with a traumatic, infected wound treated with laser phototherapy as an alternative therapy method.Case: A 3-year-old bitch Border Collie, weighing 18 kg, from the municipality of Ilhéus, Bahia, Brazil, waspresented for examination with a history of traumatic laceration of the left thoracic member. On the anamnesis, it was reported that the patient presented with laceration of the left thoracic member. The wound was cle...
ABSTRACT. Vasconcellos M., Diniz V.S., Silva P.C., Schanaider A. & Ferreira M.L. [Expectation of ... more ABSTRACT. Vasconcellos M., Diniz V.S., Silva P.C., Schanaider A. & Ferreira M.L. [Expectation of post-surgical quality of life in advanced oral neoplasms in domestic cats: case studies.] Expectativa da qualidade de vida pos-cirurgica nas neoplasias orais avancadas em felinos domesticos: estudo de casos. Revista Brasileira de Medicina Veterinaria 38(4):323-328, 2016. Faculdade de Medicina Veterinaria, Universidade Luterana do Brasil, Av. Farroupilha, 8001, Predio 60, Bairro Sao Jose, Canoas, RS 92425-900, Brasil. E-mail: tinevet@terra.com.brThe management in advanced oral cancer in cats is a challenge in veterinary medicine. Several factors, such as tumor aggressiveness, difficulty in oral reconstruction, low response to chemotherapy and limited access to radiotherapy have contributed to a short survival of time. The postoperative tumor recurrence constitutes a major cause of death. The objective of this contribution is to verify the validity of the total mandibulectomy as the only t...
To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment ... more To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment with intravenous ceftriaxone in a new rabbit model. New Zealand rabbits were treated with intravenous ceftriaxone or saline for 21 days. Ultrasound monitoring of the gallbladder was performed every seven days until the 21st day when histopathology, immunohistochemistry for proliferating cell nuclear antigen (PCNA), pro-caspase-3 and CD68, liver enzyme biochemistry, and chromatography analysis of the bile and sediments were also performed. All animals treated with ceftriaxone developed acute cholecystitis, confirmed by histopathology (P<0.05) and biliary microlithiasis, except one that exhibited sediment precipitation. In the group treated with ceftriaxone there was an increase in pro-caspase-3, gamma-glutamyl transpeptidase concentration, PCNA expression and in the number of cells positive for anti-CD68 (P<0.05). In the ceftriaxone group, the cholesterol and lecithin concentrations...
PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in t... more PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in the evaluation of bone grafts in rabbits. METHODS: The operative technique was performed in jaws, with external approach from the labial commissure to the last molar tooth. An incision about 1cm extended forward to the alveolar ridge, followed by a exposing the cervical region of the alveolar bone and dental elements was done. Thus, after extraction of first molar with forceps, the tooth socket left was filled with hydroxyapatite. The bone gain was analyzed by histopathological studies. RESULTS: The histological analyses indicated formed bone surrounding the biomaterial. CONCLUSION: The experimental model using the tooth socket of the rabbit molar is a feasible procedure for studies of bone grafts.
To verify the viability and functionality of cryopreserved thyroid autotransplantation in rats wh... more To verify the viability and functionality of cryopreserved thyroid autotransplantation in rats who underwent total thyroidectomy in the treatment of postoperative hypothyroidism. Thirty-two Wistar rats were randomly assigned into groups (G) with eight animals each: control (CG); simulation (SG); hypothyroidism (HTG) and transplanted (TG). At the beginning and in the 13th week of the experiment, serum levels of total T3, free T4, TSH and calcium were determined. In both the first and 14th weeks, scintigraphic examinations, 99m-Tc pertechnetate radioisotope biodistribution and histopathology were performed. In the 14th week, the expression of proliferating cell nuclear antigen (PCNA) and cellular apoptosis (caspase-3) were also evaluated. In the 13th week, the transplanted animals had normal serum levels of total T3 and free T4. TSH levels showed a tendency towards normality. In the 14th week, scintigraphic exams displayed graft isotopic uptake in all animals in the TG group. Histolog...
Background: Anal atresia is a congenital defect and is related to the flaw of an opening in the m... more Background: Anal atresia is a congenital defect and is related to the flaw of an opening in the membrane that separates the endoderm of the posterior intestine from the ectodermic anal membrane. This anomaly can cause debilitating disease, death, or abnormal development of the animal. Clinical presentation of urethrorectal fistula in domestic animals differs between males and females, as in the former there is passage of the urine through the anus, whereas in females there is elimination of the feces through the vulva. The absence of description of this disease in horses stimulated describing a case of surgical resolution of anal atresia, rectal bulb agenesis, and pneumovagina of an adult mare.Case: Mare with a history of defecating through the vagina. In the anamnesis, it was determined that the owner noticed the problem after observing the animal during a walk. Clinical examination verified absence of the anus with stool evacuation through the vagina, pneumovagina, and signs of cy...
Quantitative Ultrasound (QUS) is clinically used in diagnosis and monitoring of bone diseases app... more Quantitative Ultrasound (QUS) is clinically used in diagnosis and monitoring of bone diseases applied to sites with a few overlaying soft tissues. Long bones diaphysis is a frequent site of high impact fractures and the assessment is commonly made by radiographies. An ultrasound pulse-echo method was proposed to in vivo signal acquisition for long bone characterization in an animal model. Four parameters related to backscattering and reflection were calculated in both femurs of 5 rats and the results showed that their values statistically belong to the same group. This is a first indication that the proposed protocol has potential to characterize normal in vivo long bones in animal models.
The authors detail the experimental development of a technique for the reconstruction of the uret... more The authors detail the experimental development of a technique for the reconstruction of the ureter using a tubular shape, muscle flap of the abdominal wall. the preliminary results indicate the feasibility of this surgical technique.
OBJETIVO: Descreve-se a técnica de um novo um modelo de pseudoartrose em Rattus norvegicus albinu... more OBJETIVO: Descreve-se a técnica de um novo um modelo de pseudoartrose em Rattus norvegicus albinus livre de espécies patogênicas (SPF). MÉTODOS: Os animais foram aleatoriamente distribuídos em dois Grupos: Grupo 1 - Controle (n =6), Grupo 2 - Experimental (n =6). No grupo Controle realizou-se um acesso ao fêmur, seguido da síntese por planos do músculo e da pele. O grupo Experimental foi submetido à osteotomia do fêmur aduzida da confecção de um retalho pediculado de fáscia lata para interposição entre os segmentos resultantes da fratura induzida cirurgicamente. Prosseguiu-se com o alinhamento e aproximação dos segmentos ósseos, mediante uso de fios de náilon introduzidos em orifícios efetuados na extremidade proximal e distal. RESULTADOS: Estudos radiológico e anatomopatológico evidenciaram a ausência de consolidação óssea com persistência de tecido fibro-osteóide decorridos 120 dias de evolução pós-operatória. CONCLUSÃO: O modelo experimental é adequado para a formação de pseudoar...
PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the abil... more PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the ability of backscatter parameters from ultrasound signals in differentiating normal bone from those ones with pseudarthorsis. METHODS: Twelve Rattus norvegicus albinus free from pathogenic species (SPF) were randomly divided in two groups, with six animals each. In the Control group a surgical approach to the femur was made, followed by the synthesis of the muscle and skin layers. The Experimental group was submitted to an osteotomy of the femur and a vascularized flap of the fascia lata was interposed in the line of the fractured bone. Then the alignment and bone stabilization were accomplished, by using nylon stitch in U shape introduced in holes made in the proximal and distal fractured bone. Bone samples were scanned with ultrasound and signals were collected for each one to analyze the parameter Apparent Integrated Backscatter - AIB. RESULTS: Radiological and anatomopathologic studies r...
OBJETIVO: Estabelecer um modelo de insuficiência renal crônica (IRC) em coelhos, com perspectivas... more OBJETIVO: Estabelecer um modelo de insuficiência renal crônica (IRC) em coelhos, com perspectivas de seu uso para ações terapêuticas e reparadoras. MÉTODO: Foram utilizados 19 coelhos da linhagem Nova Zelândia, machos, adultos, aleatoriamente distribuídos em três Grupos: Grupo 1 - Controle (n =5), Grupo 2- Simulação (n =7), Grupo 3 - Experimental (n =7). Os animais foram anestesiados com Cetamina e Diazepam e Fentanil por via intramuscular e Sevorane, através de vaporizador. No Grupo 3 realizou-se uma nefrectomia bipolar esquerda e após quatro semanas, uma nefrectomia direita. Todas as amostras do parênquima renal foram pesadas. O Grupo 2 foi submetido a duas laparotomias, sem nefrectomia.. Em todos os grupos foram realizadas avaliações: bioquímicas com dosagens de uréia, creatinina, sódio e potássio; ultra-sonográficas abdominais; cintigráficas e histológicas. RESULTADOS: No Grupo 3 houve aumento progressivo da uréia (p=0.0001), creatinina (p=0,0001), sódio (p= 0,0002) e potássio (...
OBJETIVO: Avaliar a eficácia da técnica extra-capsular para o tratamento de ruptura do ligamento ... more OBJETIVO: Avaliar a eficácia da técnica extra-capsular para o tratamento de ruptura do ligamento cruzado anterior em cães. MÉTODOS: Foi realizada a reparação cirúrgica extra-articular, sem artrotomia do ligamento cruzado anterior, com a utilização da fáscia lata autógena para estabilização da articulação do joelho em seis animais que apresentaram claudicação grave e movimento de gaveta positivo. RESULTADOS: A técnica cirúrgica extra-articular foi eficaz com boa estabilização articular e evolução satisfatória. CONCLUSÃO: A via extra capsular com uso da fáscia lata para correção da ruptura do ligamento cruzado anterior mostrou-se útil haja vista tratar-se de um procedimento simples e de rápida execução, causando o mínimo dano tecidual e recuperação pós-operatória eficiente.
Background: Laser photobiomodulation has been used in the treatment of various injuries and disea... more Background: Laser photobiomodulation has been used in the treatment of various injuries and diseases. This promotes modulation of the inflammatory process, edema reduction and devitalized tissue regeneration. The advantages of Antimicrobial photodynamic therapy are its easy application and the absence of side effects. Other advantages are the cost of the therapy, minimal damage to animal tissue, the broad spectrum of action, and efficiency against strains resistant to antimicrobials. The aim of this study was to report the clinical and their resolution in a female dog with a traumatic, infected wound treated with laser phototherapy as an alternative therapy method.Case: A 3-year-old bitch Border Collie, weighing 18 kg, from the municipality of Ilhéus, Bahia, Brazil, waspresented for examination with a history of traumatic laceration of the left thoracic member. On the anamnesis, it was reported that the patient presented with laceration of the left thoracic member. The wound was cle...
ABSTRACT. Vasconcellos M., Diniz V.S., Silva P.C., Schanaider A. & Ferreira M.L. [Expectation of ... more ABSTRACT. Vasconcellos M., Diniz V.S., Silva P.C., Schanaider A. & Ferreira M.L. [Expectation of post-surgical quality of life in advanced oral neoplasms in domestic cats: case studies.] Expectativa da qualidade de vida pos-cirurgica nas neoplasias orais avancadas em felinos domesticos: estudo de casos. Revista Brasileira de Medicina Veterinaria 38(4):323-328, 2016. Faculdade de Medicina Veterinaria, Universidade Luterana do Brasil, Av. Farroupilha, 8001, Predio 60, Bairro Sao Jose, Canoas, RS 92425-900, Brasil. E-mail: tinevet@terra.com.brThe management in advanced oral cancer in cats is a challenge in veterinary medicine. Several factors, such as tumor aggressiveness, difficulty in oral reconstruction, low response to chemotherapy and limited access to radiotherapy have contributed to a short survival of time. The postoperative tumor recurrence constitutes a major cause of death. The objective of this contribution is to verify the validity of the total mandibulectomy as the only t...
To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment ... more To evaluate the actual incidence of both microlithiasis and acute cholecystitis during treatment with intravenous ceftriaxone in a new rabbit model. New Zealand rabbits were treated with intravenous ceftriaxone or saline for 21 days. Ultrasound monitoring of the gallbladder was performed every seven days until the 21st day when histopathology, immunohistochemistry for proliferating cell nuclear antigen (PCNA), pro-caspase-3 and CD68, liver enzyme biochemistry, and chromatography analysis of the bile and sediments were also performed. All animals treated with ceftriaxone developed acute cholecystitis, confirmed by histopathology (P<0.05) and biliary microlithiasis, except one that exhibited sediment precipitation. In the group treated with ceftriaxone there was an increase in pro-caspase-3, gamma-glutamyl transpeptidase concentration, PCNA expression and in the number of cells positive for anti-CD68 (P<0.05). In the ceftriaxone group, the cholesterol and lecithin concentrations...
PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in t... more PURPOSE: The aim of this study was to describe an experimental surgical technique to be used in the evaluation of bone grafts in rabbits. METHODS: The operative technique was performed in jaws, with external approach from the labial commissure to the last molar tooth. An incision about 1cm extended forward to the alveolar ridge, followed by a exposing the cervical region of the alveolar bone and dental elements was done. Thus, after extraction of first molar with forceps, the tooth socket left was filled with hydroxyapatite. The bone gain was analyzed by histopathological studies. RESULTS: The histological analyses indicated formed bone surrounding the biomaterial. CONCLUSION: The experimental model using the tooth socket of the rabbit molar is a feasible procedure for studies of bone grafts.
To verify the viability and functionality of cryopreserved thyroid autotransplantation in rats wh... more To verify the viability and functionality of cryopreserved thyroid autotransplantation in rats who underwent total thyroidectomy in the treatment of postoperative hypothyroidism. Thirty-two Wistar rats were randomly assigned into groups (G) with eight animals each: control (CG); simulation (SG); hypothyroidism (HTG) and transplanted (TG). At the beginning and in the 13th week of the experiment, serum levels of total T3, free T4, TSH and calcium were determined. In both the first and 14th weeks, scintigraphic examinations, 99m-Tc pertechnetate radioisotope biodistribution and histopathology were performed. In the 14th week, the expression of proliferating cell nuclear antigen (PCNA) and cellular apoptosis (caspase-3) were also evaluated. In the 13th week, the transplanted animals had normal serum levels of total T3 and free T4. TSH levels showed a tendency towards normality. In the 14th week, scintigraphic exams displayed graft isotopic uptake in all animals in the TG group. Histolog...
Background: Anal atresia is a congenital defect and is related to the flaw of an opening in the m... more Background: Anal atresia is a congenital defect and is related to the flaw of an opening in the membrane that separates the endoderm of the posterior intestine from the ectodermic anal membrane. This anomaly can cause debilitating disease, death, or abnormal development of the animal. Clinical presentation of urethrorectal fistula in domestic animals differs between males and females, as in the former there is passage of the urine through the anus, whereas in females there is elimination of the feces through the vulva. The absence of description of this disease in horses stimulated describing a case of surgical resolution of anal atresia, rectal bulb agenesis, and pneumovagina of an adult mare.Case: Mare with a history of defecating through the vagina. In the anamnesis, it was determined that the owner noticed the problem after observing the animal during a walk. Clinical examination verified absence of the anus with stool evacuation through the vagina, pneumovagina, and signs of cy...
Quantitative Ultrasound (QUS) is clinically used in diagnosis and monitoring of bone diseases app... more Quantitative Ultrasound (QUS) is clinically used in diagnosis and monitoring of bone diseases applied to sites with a few overlaying soft tissues. Long bones diaphysis is a frequent site of high impact fractures and the assessment is commonly made by radiographies. An ultrasound pulse-echo method was proposed to in vivo signal acquisition for long bone characterization in an animal model. Four parameters related to backscattering and reflection were calculated in both femurs of 5 rats and the results showed that their values statistically belong to the same group. This is a first indication that the proposed protocol has potential to characterize normal in vivo long bones in animal models.
The authors detail the experimental development of a technique for the reconstruction of the uret... more The authors detail the experimental development of a technique for the reconstruction of the ureter using a tubular shape, muscle flap of the abdominal wall. the preliminary results indicate the feasibility of this surgical technique.
OBJETIVO: Descreve-se a técnica de um novo um modelo de pseudoartrose em Rattus norvegicus albinu... more OBJETIVO: Descreve-se a técnica de um novo um modelo de pseudoartrose em Rattus norvegicus albinus livre de espécies patogênicas (SPF). MÉTODOS: Os animais foram aleatoriamente distribuídos em dois Grupos: Grupo 1 - Controle (n =6), Grupo 2 - Experimental (n =6). No grupo Controle realizou-se um acesso ao fêmur, seguido da síntese por planos do músculo e da pele. O grupo Experimental foi submetido à osteotomia do fêmur aduzida da confecção de um retalho pediculado de fáscia lata para interposição entre os segmentos resultantes da fratura induzida cirurgicamente. Prosseguiu-se com o alinhamento e aproximação dos segmentos ósseos, mediante uso de fios de náilon introduzidos em orifícios efetuados na extremidade proximal e distal. RESULTADOS: Estudos radiológico e anatomopatológico evidenciaram a ausência de consolidação óssea com persistência de tecido fibro-osteóide decorridos 120 dias de evolução pós-operatória. CONCLUSÃO: O modelo experimental é adequado para a formação de pseudoar...
PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the abil... more PURPOSE: To propose a novel model of pseudarthrosis in a small animal and to investigate the ability of backscatter parameters from ultrasound signals in differentiating normal bone from those ones with pseudarthorsis. METHODS: Twelve Rattus norvegicus albinus free from pathogenic species (SPF) were randomly divided in two groups, with six animals each. In the Control group a surgical approach to the femur was made, followed by the synthesis of the muscle and skin layers. The Experimental group was submitted to an osteotomy of the femur and a vascularized flap of the fascia lata was interposed in the line of the fractured bone. Then the alignment and bone stabilization were accomplished, by using nylon stitch in U shape introduced in holes made in the proximal and distal fractured bone. Bone samples were scanned with ultrasound and signals were collected for each one to analyze the parameter Apparent Integrated Backscatter - AIB. RESULTS: Radiological and anatomopathologic studies r...
OBJETIVO: Estabelecer um modelo de insuficiência renal crônica (IRC) em coelhos, com perspectivas... more OBJETIVO: Estabelecer um modelo de insuficiência renal crônica (IRC) em coelhos, com perspectivas de seu uso para ações terapêuticas e reparadoras. MÉTODO: Foram utilizados 19 coelhos da linhagem Nova Zelândia, machos, adultos, aleatoriamente distribuídos em três Grupos: Grupo 1 - Controle (n =5), Grupo 2- Simulação (n =7), Grupo 3 - Experimental (n =7). Os animais foram anestesiados com Cetamina e Diazepam e Fentanil por via intramuscular e Sevorane, através de vaporizador. No Grupo 3 realizou-se uma nefrectomia bipolar esquerda e após quatro semanas, uma nefrectomia direita. Todas as amostras do parênquima renal foram pesadas. O Grupo 2 foi submetido a duas laparotomias, sem nefrectomia.. Em todos os grupos foram realizadas avaliações: bioquímicas com dosagens de uréia, creatinina, sódio e potássio; ultra-sonográficas abdominais; cintigráficas e histológicas. RESULTADOS: No Grupo 3 houve aumento progressivo da uréia (p=0.0001), creatinina (p=0,0001), sódio (p= 0,0002) e potássio (...
OBJETIVO: Avaliar a eficácia da técnica extra-capsular para o tratamento de ruptura do ligamento ... more OBJETIVO: Avaliar a eficácia da técnica extra-capsular para o tratamento de ruptura do ligamento cruzado anterior em cães. MÉTODOS: Foi realizada a reparação cirúrgica extra-articular, sem artrotomia do ligamento cruzado anterior, com a utilização da fáscia lata autógena para estabilização da articulação do joelho em seis animais que apresentaram claudicação grave e movimento de gaveta positivo. RESULTADOS: A técnica cirúrgica extra-articular foi eficaz com boa estabilização articular e evolução satisfatória. CONCLUSÃO: A via extra capsular com uso da fáscia lata para correção da ruptura do ligamento cruzado anterior mostrou-se útil haja vista tratar-se de um procedimento simples e de rápida execução, causando o mínimo dano tecidual e recuperação pós-operatória eficiente.
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