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Radioimmunoassay Ca1

The document discusses Radioimmunoassay (RIA), a sensitive in vitro assay technique used to measure antigen concentrations, such as hormone levels in blood, through immunological reactions. It outlines the principles of RIA, including immune reaction, competitive binding, and measurement of radio emission, as well as the procedure for conducting the assay. RIA is highlighted as an effective and accurate method for quantifying antigen concentrations in various pharmacological applications.

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8024 Sakasi Halder
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9 views6 pages

Radioimmunoassay Ca1

The document discusses Radioimmunoassay (RIA), a sensitive in vitro assay technique used to measure antigen concentrations, such as hormone levels in blood, through immunological reactions. It outlines the principles of RIA, including immune reaction, competitive binding, and measurement of radio emission, as well as the procedure for conducting the assay. RIA is highlighted as an effective and accurate method for quantifying antigen concentrations in various pharmacological applications.

Uploaded by

8024 Sakasi Halder
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 6

GURU NANAK INSTITUTE OF PHARMACEUTICAL SCIENCE AND

TECHNOLOGY (An Autonomous Institute)

TOPIC : RADIO IMMUNO ASSAY (PRINCIPLE & WORKING)

NAME OF THE STUDENT : MOULY MITRA


PAPER NAME: ADVANCED SPECTRAL
ANALYSIS
PNR NUMBER: 186112401006

PAPER CODE: MPT 2031


ACADEMIC SESSION : 2024-2025

Affiliated to Maulana Abul Kalam Azad University of


INTRODUCTION

Immuno Assays

Immunoassays are the biological tests measure the concentration of substance in a biological liquid
typically serum or urine using immunological reaction of an antibody or anti antibodies to its antigen.

Radio immunoassay (RIA)


Radioimmunoassay (RIA) is a highly sensitive in vitro assay technique used to measure concentrations of
substances, usually measuring antigen concentrations (for example, hormone levels in blood) by use of
antibodies.

Page no- 1
PRINCIPLE

The three key principles involved in RIA are:


1) Immune reaction
2) Competitive binding or competitive displacement reaction
3) Measurement of radio emission

Page no- 2
Procedure

Take a micro tighter plate precoated with antibody

Add a radio labeled antigen (Hot antigen)

The hot antigen will bind with pre coated antibody

Remove the unbound hot antigen by washing by buffered solution

Add a patient sample which contain cold antigen (unlabeled antigen)

This cold antigen will compete with radio labeled antigen and replace
them

This replacement of cold antigen release them away from the antibody

Production of radioactivity

Measurement of radioactivity by Gamma Counter

Page no-3
CONCLUSION
SUMMARY

•RIA is an in-vitro assay technique used for the determination of Antigen concentration
present in a given sample.
•RIA have been successfully applied to a wide variety of pharmacological agents.
•RIA is an effective, precise and accurate method of quantifying concentrations of an antigen.

Page no-4
REFERENCE

1. Advanced Instrumentation Techniques by Dr. S. Ravichandran, Dr. Narendra Mulchand Gowekar


p.n: 178-204
2. Kim JH, Lee SY, Lee SK. Development of novel lab-on-a-chip platform for high-throughput
radioimmunoassay. Applied Radiation and Isotopes. 2021 Feb 1;168:109526.
3. Chappell MC, Pirro NT, South AM, Gwathmey TM. Concerns on the specificity of commercial
ELISAs for the measurement of angiotensin (1–7) and angiotensin II in human plasma.
Hypertension. 2021 Mar 3;77(3):29-31.

Page no- 5

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