CSI 201
Skills Lab 2
LUMBAR PUNCTURE
DARYL P. LOFASO, Ph.D.,M.Ed., RRT
Indications for Lumbar Puncture
To obtain CSF for Laboratory Examination
Administer Medications into subarachnoid space
Perform Myelography
Measure ICP
Dx CNS infections and Inflammatory Diseases
LP Contraindications
Infectionsat puncture site
Suspected CNS mass lesion
Increased ICP (relative)
Coagulation disorder (relative)
Lumbar Puncture Checklist
Check the indications
Know the risks and how to deal with serious
complications
Explain the issues to the patient
Check for allergy to lidocaine or iodine
Consider bleeding tendency / risks
Lumbar Puncture Equipment
Consent form (Signed)
Lab request forms and specimen bottles
Sterile Gloves, sterile gown, mask & eye
protection
Local anesthetic
LP Tray
Lumbar Puncture
What structures or layers with the needle pass
through before reaching the subarahnoid space?
Skin
Superficial fascia
Superspinous ligament
Interspinous ligament
Ligamentum flavum
Epidural space containing fatty
areolar tissue and the internal
vertebral venous plexus
Dura mater
Arachnoid mater
Subarachniod space
Invasive/Non-Invasive
Procedure Consents
Date/Time of consent
Indications
Alternativetreatment
Risk/Benefits
Documentation that all questions have been answered
Signature of patient or authorized representative before
procedure performed & appropriate witness –
Date/Time
Test CSF
Appearance
Protein
Sugar
Serology
Cell Count
Bacterial or Fungal
Cultures
Helpful Hints
What is the most important step before
performing a Lumbar Puncture?
Answer: Positioning the Patient
Culture Tubes
Tube 1: Gram stain, (AFB, fungal)
Tube 2: Glucose and Protein
Tube 3: Cell count (RBC, WBC with differentials)
Tube 4: Hold for possible (VDRL, India ink,
electrophoresis, antigen panel)
* (Tube sequence may varies per hospital)
Complications to LP
Spinal Headache
Trauma
Herniation
Infection
Hemorrhage
Bloody Tap
Invasive/Non-Invasive
Procedure Note
Proper Patient Identification
Site Verification
Pre / Post Medication for pain control
Intra / Post procedure monitoring
Complications, if any
Management of Complications
Universal Precautions
AllPatients are potentially infectious.
Good Handwashing is the key to reducing
nosocomial infections
Wash before and after patient contact
Wear a mask, eye protection, face shield, and
gown when needed