Professional Documents
Culture Documents
Gene Banks
Gene Banks
banks
Field gene banks have been established in many countries for different crops.
Eg of Field gene banks
Malaysia- Oil palm has been conserved on 500
hectares
Disadvantages
1. Field gene banks cannot cover the entire genetic diversity
of a species -only a fraction of the full range of diversity of a
species.
2. The germplasm in field gene banks is exposed to
pathogens and insects and sometimes is damaged by
natural disasters such as bushfires, cyclones, floods, etc.
3. Maintenance of germplasm in the field gene banks is
costly affair
2. Seed gene banks :
A place where germplasm is conserved in the form of seeds is called seed gene
banks.
However, seeds of all crops can not be stored at low temperature in the seed
banks. Only orthodox species can be conserved not recalcitrant sps
Types of seed gene banks
(1) short term, (2) medium term, and (3) long term.
Advantages of gene banks :
1) Large number of germplasm samples or entire variability can be conserved in a
very small space.
2) In seed banks, handling of germplasm is easy
3) Germplasm is conserved under pathogen and insect free environment
Disadvantages:
1) Recalcitrant species cannot be stored in seed banks
2) Failure of power supply may lead to loss of viability and there by loss of
germplasm
3) Requires periodical evaluation of seed viability. After some time
multiplication is essential to get new or fresh seeds for storage.
Conserving germplasm in vitro
Merits
1. Genotypes of the accessions can be conserved indefinitely free from
diseases and pests.
2. They can be used for such crops, which either do into produce seeds
or produce recalcitrant seeds.
3. Subculture becomes necessary only after relatively long periods
(every 6-36 months).
4. Regeneration i.e., subculturing, requires a comparatively very short
time.
5. The chief merit is that it can be used for the conservation of
germplasm of vegetatively propagated crops and tree species.
[In addition, cuttings, bulbs and tubers can be maintained under controlled
humidity and temperature conditions]
Demerit
However, this approach is practical only for the short and medium term storage,
and it should be used in conjunction with a field gene bank.
4. Cell and Organ Gene Banks
Refers to a germplasm collection based on
cryopreserved (at -1960C in liquid nitrogen)
embryogenic cell cultures, shoot-tips and
or somatic/zygotic embryos