What are Bacteria?
• Bacteria are PROKARYOTES
     – The smallest known living cells
                                     They are found
 Bacteria on
head of a pin                         everywhere!!
      Starr, 317
Did you know? There are over 80
species of bacteria in your mouth!    Bacteria in dental plaque
                                            microbeworld.org
Some cause disease
We call these “pathogens”
            Anthrax, as seen by Koch
                                microbeworld.org
But most are
  beneficial
  Bacteria ferment cheese
                 Schraer, 641
Characteristics of Bacteria
               Most have a cell wall
               Many have a capsule (jelly or
                 slimy coating outside the cell
                 wall, for protection)
               They have a single, circular
                 chromosome.
               Some have plasmids (tiny
                 rings of DNA separate
                 from the chromosome.)
               Cytoplasmic Pili help them
Schraer, 632
                 talk to other cells.
 Many can MOVE
Some have flagella - made of
   rope-like proteins, not
        microtubules.
                                            Salmonella
Some slide on a slimy secretion.            www.iwate.jp
                      Many can form dormant
                      cells called endospores
                      to survive harsh conditions.
                      Streptomyces spores
Three basic
  shapes
• Spherical – coccus
• Rod – bacillus
• Coiled - spirillum
                       Schraer, 633
                   Simple Colonies
Staphylo = clusters
                                  Strepto = chains
    Staphylococcus
        wisc.edu
                                      Streptobacillus
Diplo = double
             Diplococcus
                   cat.cc.md.us
  Binary fission                    (for a quick clone)
Normal bacterial reproduction
  1) chromosome replicates
  2) copies separate as cell
        wall lengthens
  3) cell membrane pinches in
  4) septum/new wall grows
  5) cells divide
                                         Steps in binary fission
                                                maricopa.edu
          Bacillus dividing by fission
                SixKingdoms.html
How Bacteria Populations Grow!!
        Growth Curve (in Culture)
                                    Schraer, 634
         See Fission in Action
•
• Did you know? In ideal conditions, some
    species can divide every 10 MINUTES.
          What stops them?
       They run out of food or space,
    or wastes build up and poison them.
        Metabolic Needs
• Respiration
 – Most are obligate aerobes.
 – Others are facultative or obligate
   anaerobes.
 - Anaerobes make a variety of fermentation
   products:
      - acids, alcohols, methane gas
      - food products
                Nutrition
Most are heterotrophs
      - saprobes or parasites
Autotrophs
  -photosynthetic or chemosynthetic
Did you know? Chemosynthetic bacteria are
the base of the food chain at ocean floor vents.
       Little is Better!!
Metabolism is FAST
  Bacteria can absorb nutrients and     secrete
 wastes rapidly because of high
    surface-to-volume ratio
Did you know? Lactose fermenters break down
 10,000 times their weight in lactose in an
 HOUR!
  Two Kingdoms of Bacteria
• Kingdom Archaebacteria
 - “Ancient”, most primitive
         earliest known form of life
  -                                    Fluorescent micrograph
 •Kingdom Eubacteria                          of an archaeon
      - includes bacteria and
                                           microbeworld.org
          cyanobacteria (blue-green)
       Kingdom Archaebacteria
         Why a separate kingdom?
Archae differ chemically from other bacteria.
1) cell wall - different amino acids and sugars.
         Eubacteria have peptidoglycan
    Archaebacteria have varied polysaccharides
              but not peptidoglycan.
2) membrane lipids
3) ribosomes
4) enzymes - - - - - - - - - - - - >
5) cytochromes
6) gene sequences . . . And MORE
                                                   RNA polymerase
   Archae are extremophiles
      Live in habitats like early earth
        Too harsh for most organisms
1) methanogens – decomposers,
   live in intestines, swamps & bogs
   sewage treatment
2) Halophiles – “love salt”
    Great Salt Lake, Dead Sea
3) Thermophiles – hot springs, geysers
                                          Starr,635
4) Acidophiles – Guess where?
          Kingdom Eubacteria
Photosynthetic – 2 groups
  1) cyanobacteria (aerobes)
       – Have chlorophyll a
           and phycocyanin (blue)
       – Other colors, too
       – Most live in fresh water
       – Others live in salt water,
               soil and lichens
                                      Starr, 315
              Nostoc
              Schraer, 637
    More photosynthetics
2) green-sulfur and purple bacteria
     - anaerobic
     - colors range from pink to black
    - photosynthesize without water
     - make no oxygen
     - live in pond and sea mud
Chemosynthetic
make glucose using energy
 from chemical compounds
                      Tube worms at ocean vent
                       Fed by chemoautotrophs
                                                 Starr, 745
Nitrogen-fixing
 Fix nitrogen in special
cells called heterocysts
                Legume roots – nodules contain
                  nitrogen-fixing bacteria          Starr, 314
   Heterotrophic Bacteria
Many groups and types, but divided into
        two classes by GRAM STAIN
                                             Schraer
                                              637
   Gram positive         Gram negative
      Purple          Pink - Cell wall resists stain
  Respond to normal      Harder to treat if
     antibiotics            pathogenic
   Biologic Importance of
           Bacteria
1) Essential to nutrient cycling
2) Decomposers – in soil, inside animals
       Enterobacteria – live inside us,
       break down waste, make vitamins
3) Process foods – cheese, yogurt, pickles
4) Some MAKE antibiotics (streptomyacin)
5) Used in cell and molecular research,
     genetic engineering, medical research..
                              Sources
•   Schraer and Stoltz, Biology, the Study of Life, 7th ed. Prentice-Hall, 1999
•   Starr, Cecie, Biology, Concepts and Application, Wadsworth Group, 2003
•   Fission www.emc.maricopa.edu/faculty/farabee/BIOBK/BioBookmito.html
     www.biology.hawaii.edu/bio171/Notes/Bacteria/page6.htm
•   Archaebacteria http://biology.com/learning/archaea/introduction.html
•   Staphylococcus http://www.bact.wisc.edu/Bact330/lecturestaph
•   Conjugation http://tidepool.st.usm.edu/crswr/bactconjug.html
•   Legume nodules http://www.danieldeepak.com/bacteria.htm
•   Salmonella http://www.office.pref.iwate.jp/~hp1002/eiseika/salmonella.jpg
•   Bacteria reproducing
    http://marshallteachers.sandi.net/teacher_sites/mcquillan/04.Classification/Re
    adings/SixKingdoms.html
•   Dental plaque
    http://www.microbeworld.org/htm/aboutmicro/microbes/types/.htm
•   Fission time-lapse http://www.cellsalive.com/ecoli.htm
•   Diplococcus http://www.cat.cc.md.us/courses/bio141/labmanua/lab16/diplo.html