Development of Antibiotic Resistance
Through the Food We Eat
Ramos, Ian Pempe N.
Bio 198
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Microorganisms are Ubiquitous
                                3
How can we be harmed?
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Dangers brought by Microorganisms
- They serve as pathogens which causes diseases
  e.g. Cholera, Typhoid
- Causes diseases even on plants
  e.g. citrus canker
- Development of Antibiotic Resistance
                                                  5
Methodology
          Interview          Stable isotopes              Antibiotic resistance test
 - Experience          - Stable isotopes of 13C and   -Two bacterial strains of
                       15N were analyzed in corals    Bacillus niabensis and Bcillus
 - Education           along a gradient from the      pumilus
                       farms
 - Feeding practices                                  -    Disc Diffusion method
                                                      -    Tetracycline, Rifampicin
                                                           and Vancomycin
                                                                                       6
Results
Stable isotopes   Antibiotic Resistance test
                                               7
How do we acquire Antibiotic
Resistance?
Transduction, Transformation, Conjugation
                                            8
Conjugation
              Involves physical contact
              between donor and recipient
              cells and can mediate the
              transfer of genetic material
              between domains.
                                         9
Transduction
               Introduction of new genetic
               material into a bacterium by a
               bacteriophage that has replicated
               within a donor microorganism
               and packaged random DNA
               fragments
                                                   10
Transformation
                 Involves the uptake of naked DNA
                 from the environment and has the
                 potential to transmit DNA between
                 very distantly related organisms.
                                                 11
What are the things we do that
make us acquire antibiotic
resistance?
                                 12
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Results
Out of 59 strains of Vibrio parahaemolyticus
-   98.3% was resistant to Ampicillin
-   62.7% was resistant to Cephalothin
-   76.3% was resistant to Streptomycin
-   61.0% was resistant to Rifampin
-   100% was resistant to Vancomycin
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Title
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Methodology
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Results
    - Butter 4.86 µg kg1 , sour cream 3.5 µg kg1 , white cheese 2.36 µg kg1
    and whey 0.14 µg kg1. From the results obtained, it was observed that
    CAP is more accumulated in dairy products with high fat content
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Conclusion
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THANK YOU
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