[go: up one dir, main page]
Scribd
Upload
147 views9 pages

PBL 3 - SLE (Diagnostic Tests)

The document summarizes various diagnostic tests used to evaluate systemic lupus erythematosus (SLE). It describes that a CBC may show leukopenia and thrombocytopenia in 50% of patients. Urinalysis can detect protein, white blood cells, and red blood cells. Complement levels are usually low and autoantibody tests like anti-nuclear antibody (ANA), anti-dsDNA, anti-Sm, and anti-phospholipid antibodies help diagnose SLE. A positive ANA has high sensitivity but only moderate specificity for SLE. Kidney and heart imaging tests may also provide diagnostic information.

Uploaded by

Ainur Abdrakhmanova
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
147 views9 pages

PBL 3 - SLE (Diagnostic Tests)

The document summarizes various diagnostic tests used to evaluate systemic lupus erythematosus (SLE). It describes that a CBC may show leukopenia and thrombocytopenia in 50% of patients. Urinalysis can detect protein, white blood cells, and red blood cells. Complement levels are usually low and autoantibody tests like anti-nuclear antibody (ANA), anti-dsDNA, anti-Sm, and anti-phospholipid antibodies help diagnose SLE. A positive ANA has high sensitivity but only moderate specificity for SLE. Kidney and heart imaging tests may also provide diagnostic information.

Uploaded by

Ainur Abdrakhmanova
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PPTX, PDF, TXT or read online on Scribd
You are on page 1/ 9

Diagnostic tests

Ainur Abdrakhmanova
Diagnostic tests
CBC (leukopenia, thrombocytopenia in
50%)
Chemistry panel: liver and kidney function
tests
ESR: inflammation marker, used to monitor
Urinalysis: presence of protein,
wbc, rbc, cellular casts
BUN/creatinine: high
Complement: low
CRP: inflammation marker
Anti phospholipid Ab: in 60%
Anti - Ro(SSA) and Anti La(SSB): against RNA
proteins, in 35-40% and 15% respectively
Anti-Sm Ab: in 25-30%, specificity is 99%
Anti-dsDNA: in 25-85%, specificity is almost
100%, titers correlate with disease activity

DNA binding and C3 94% (predictive value)

X - ray: abnormal shadows (fluid, inflammation


in the lungs)
Echocardiogram: real time image of beating
heart
Kidney biopsy
ANA test
Sensitivity: 93-95%
Specificity: 57%
Healthy people positive low titer
Titer not used to assess the disease
activity
ANA test

Immune ELISA Immunoblots


fluorescence (IF)
1. Pattern of
separation of
fluorescence cytoplasmic
generic Ag and Nu Ag by
2. Substrate used
3. ANA titer (1:160 specific MW
- In and
3% >)false positive
similar to IF multiple Ag transfer to
- Some patterns less on the strip/membran
defined plate e
- Difficult to specify Ab - Highly sensitive Incubation
- Used mainly for - False positive results with control
screening - Screening and and pt. serum
diagnosis
+ if band

- Only linear epitopes can be


detected
ANA pattern
speckled homogeneous

Rim (peripheral) nucleolar


Reference
Autoimmunity Newsletter Intro Part 1. 2011. Retrieved from http://
www.lancet.com.ng/index.php/pathology-centre/pathology-
newsletters/chemical-pathology/autoimmunity-newsletter-
introduction- part-1/
Magrey M., Abelson A. 2010. Laboratory evaluation of rheumatic
diseases. Retrieved from http://
www.clevelandclinicmeded.com/medicalpubs/diseasemanagement/r
heumatology/laboratory-evaluation-rheumatic-diseases/
Kumar Y., Bhatia A., Minz R.W. 2009. Antinuclear antibodies and their
detection methods in diagnosis of connective tissue diseases: a
journey revisited. Retrieved from https://
diagnosticpathology.biomedcentral.com/articles/10.1186/1746-1596-4
-1
Lab test for lupus. Retrieved from http://
www.webmd.com/lupus/guide/laboratory- tests-used-diagnose-
evaluate-sle#1
Lupus. Test and diagnostics. Retrieved from http://
www.mayoclinic.org/diseases- conditions/lupus/basics/tests-
diagnosis/con-20019676

You might also like