FemtoJet

Operating Manual

Eppendorf AG
22331 Hamburg · Germany
Phone: +49 40-5 38 01-0
Fax: +49 40-5 38 01-556
e-mail: eppendorf@eppendorf.com
Internet: www.eppendorf.com
Application Hotline:
Phone: +49 180-3 66 67 89
e-mail:
application-hotline@eppendorf.de
Brinkmann Instruments, Inc.
One Cantiague Road,
P.O. Box 1019
Westbury, New York 11590-0207
(USA)
Phone: 800-645-3050
Fax: 516-334-7506
e-mail: info@brinkmann.com
Internet: www.brinkmann.com
Printed in Germany eppendorf is a registered trademark
Contents

1 Safety precautions and application . . . . . . . . . . . . . . . . . . . 3

2 Device description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Short description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Startup. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Display, variable regulators and keypad . . . . . . . . . . . . . . . . 12
3.1.1 Setting the injection parameters . . . . . . . . . . . . . . . . . . . . . . 12
3.1.2 Variable regulators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.1.3 Keypad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.2 Hand control / foot control . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.3 Connecting the capillary holder . . . . . . . . . . . . . . . . . . . . . . . 18
3.4 Information on working practices. . . . . . . . . . . . . . . . . . . . . . 20
3.4.1 Compensation pressure pc . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.4.2 Injection pressure pi. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.4.3 Injection time ti . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.4.4 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.4.5 Manual or automatic injection . . . . . . . . . . . . . . . . . . . . . . . . 23
3.4.6 Constant pressure/constant working pressure . . . . . . . . . . . 23
3.4.7 General note . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3.5 The first injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
3.6 Connecting an Eppendorf Micromanipulator . . . . . . . . . . . . . 25
3.7 Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.7.1 Changing the capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.7.2 Setting the pressure unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
3.7.3 Switching on/off the loudspeaker. . . . . . . . . . . . . . . . . . . . . . 27
3.7.4 Switching on/off the background illumination of the display . 27
3.7.5 Constant pressure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
3.7.6 Dewatering the pressure accumulator . . . . . . . . . . . . . . . . . . 28

4 Care and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

4.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.2 Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
4.3 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

No part of this publication may be reproduced without the prior permission of the copyright owner.
Copyright 2000 by Eppendorf AG, Hamburg

B 5247 900.011-03/0201
Contents

5 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.1 Potential errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.1.1 Self-test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.2 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.3 Error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

7 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

8 Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

Appendix A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Operation via a computer . . . . . . . . . . . . . . . . . . . . . . . . . . 45
A.1 Description of interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
A.2 Commands and queries . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

EC Conformity Declaration

2
1 Safety precautions and application

● Observe operating instructions!

● Before plugging in the device, please compare your voltage

requirements with the specifications on the identification plate!

● The unit must be connected to an earthed socket!

● Electrical connections may be effected only when the device is

switched off!

● The unit may be opened only by authorized service personnel!

Potentially lethal voltage inside the unit.

● Do not use the unit if damaged. This applies in particular when the
mains cable is damaged.

● Electrical connection with devices not expressly mentioned in this

operating manual is permitted only after obtaining prior approval from
the manufacturer!

● If the unit is not used in accordance with purpose for which it is

intended, its protective function may be impaired.

● Caution!
Take care when mounting glass capillaries or tools!
Mounting must always take place without the use
of pressure! Capillaries which have been mounted
incorrectly may break loose under pressure. Never
point mounted capillaries at persons.

● Caution!
Take care when using glass capillaries! During
mounting or when placed upon a hard surface, the
glass capillaries may break and glass splinters may
cause injury. It is therefore essential to wear eye
protection!

3
1 Safety precautions and application

● In the case of independently-manufactured capillaries, ensure that the

outer diameter of the capillary corresponds to the specifications of the
capillary grip head.

● In the case of extended periods of non-use, the unit should be

depressurized using the key and switched off before being
stored!

● Use the unit only for the described purpose! The unit and in particular
the high pressure may not be used for purposes other than
microinjection!

● Use only original Eppendorf accessories. Only original accessory

parts may be used. The only exceptions are auxiliary pieces of
equipment recommended by the manufacturer. Otherwise all claims
under guarantee and liability become void immediately. Use other
accessories only if you have a certificate from Eppendorf AG
confirming technical safety in use.

● The responsibility for the correct functioning of the device passes

onto the owner or user if the device is maintained or serviced
improperly by persons not belonging to the Eppendorf service team or
if used for a purpose other than that intended. Eppendorf AG is not
liable for damage occurring from non-observance of these notes.
Guarantee and conditions of liability of the terms of sale and delivery
of Eppendorf AG are not expanded by the above notes.

● The Eppendorf AG reserves the right to make technical alterations to

this device!

4
2 Device description

The FemtoJet enables minute quantities of liquid to be injected into cells.

The relevant parameters (injection pressure pi and injection time ti)
are set using the variable regulators. The key or connected hand or
foot control or the INJECT key of the connected micromanipulator are
used to trigger these two parameters. If no injections are being performed,
compensation pressure pc is applied permanently.

5
2 Device description

2.1 Overview

1 Display
– Displays relevant parameters during injection or setting
of parameters.
– Guides operator through functions.
2 Keypad
– To trigger actions.
– To call up the function menu.
– To switch to and from Standby mode.
3 Variable regulators
– To set parameters injection pressure pi, injection time ti and
compensation pressure pc.
– To select functions and parameters in Function mode.
4 Tube connection
– To connect the pressure tube to the device via the bayonet joint.

6
2 Device description

Standard accessories:
1 Pressure tube, 2 m
1 Universal capillary holder with grip head for capillary diameters
of 1.0 mm and adapter for Femtotips
1 Connecting cable (Interface Cable 5171) to the Micromanipulator 5171
or InjectMan
1 Hand control for triggering injection and the Clean function
1 Femtotips, Femtotips II, Microloader and CELLocate demo pack
1 Mains cable
1 Operating manual

Optional accessories:
1 Foot control for triggering the Inject function

7
2 Device description

2.2 Short description

Display
Display of all relevant parameters and information.

Function keys

Clean
To apply the maximum pressure for cleaning the capillary
to the tube outlet.

Auto / Manu
To switch between manual and automatic time-controlled
injection.

Count
To reset to zero the counter for injections which have been
actuated.

Inject
To execute automatic or manual injection.

Menu
To switch the device to menu control; selected functions
may be executed.

Standby
When the key is pressed briefly:
To switch the device between operating mode and standby
mode.
When the key is held down:
As above, but also deairs the pressure accumulator.

8
2 Device description

Variable regulator
In the normal operating mode, the parameters for injection are set by the
three variable regulators. The arrangement of the variable regulators
corresponds to the arrangement of the parameters in the two bottom lines
of the display:
From left to right: injection pressure pi, injection time ti and compensation
pressure pc.
In other operating modes, the variable regulators have either the
significance indicated in the display or no significance at all.

Tube connection
The pressure tube is connected to the device via a bayonet joint.

9
2 Device description

2.3 Startup

3
3
2

Diagram of rear of device

– Electrical connection is effected via the mains socket with integrated

mains switch (1).

– Connection to an Eppendorf manipulator designed for injection is

effected via the 15-pin interface (2).

– Optional switches (e.g. foot control, hand control) or a computer can

be connected to the 9-pin interfaces.

Switching on the device

Set the mains switch on the rear of the device to "I". The unit is switched
on, the display lights up, the unit runs through a warm-up routine and is
then ready for operation. The injection mode is shown in the display.
After being switched on, the unit runs through an initialization phase and
supply pressure is built up. This takes approximately three minutes.

10
2 Device description

Switching off the device

If it is not going to be used for an extended period, the device should
be depressurized via the switch-off routine using the key. This
guarantees a long service life of the device (see Section 3.1.3).
The switch-off routine ensures that the system is dewatered and deaired
correctly. Do not disconnect the device from the mains supply until
dewatering and deairing have taken place. If only the mains switch is
actuated, the existing pressure remains in the system.

11
3 Mode of operation

3.1 Display, variable regulators and keypad

Before operating the device for the first time, users should read this
section carefully. This section describes operation of the device only.
Handling of the injection capillaries is described in Section 3.3.

AUTO n= 0 Display
The display shows the following:
pi= 150 hPa 1st line:
pi[hPa] ti[s] pc[hPa] injection mode AUTO or MANU,
150 0.2 50 counter n for number of injections
performed

AUTO n= 0 2nd line (bold):

The most-recently set parameter or
pi= 215 PSI relevant parameter

pi[PSI] ti[s] pc[PSI] Bottom lines:

2.15 0.2 0.72 Parameters which affect injection
Compensation pressure pc which is
applied permanently in the normal
mode is displayed as an actual
measured value.
The arrangement of the three
parameters shown, pi, ti, pc,
corresponds to the arrangement of
the variable regulators.

3.1.1 Setting the injection parameters

The injection parameters have to be adapted to the injection capillaries
used and the cell type and liquid which are to be injected.
The following injection parameters can be set using the variable
regulators:
Injection pressure pi 0 – 6,000 hPa (0.00 – 87.0 psi)
Injection time ti 0.1 – 99.9 s
Compensation pressure pc 0 – 6,000 hPa (0,00 – 87.0 psi)

The settings are in increments of 1 hPa/0.1 s. The display can show hPa
or psi, as required. The setting and internal pressure conversion are
always performed in hPa. Rounding for the display in psi can result in
increments of 0.01 to 0.02 psi.
The display of pressure in psi varies according to the size of the value
(e.g.: 9.99 may also appear in the display as 10.0).

12
3 Mode of operation

Slight deviations between the nominal value (specified value) and

displayed compensation pressure pc (actual value) may occur for
technical reasons. The relevant value is the actual value measured.
The following settings are used as a
AUTO n= 0
basis for initial injection experiments
using Femtotip / Femtotip II: pc= 50 hPa
pi[hPa] ti[s] pc[hPa]
Display: 150 0.5 50

pi left-hand variable regulator: 150 hPa

ti central variable regulator: 0.5 s
pc right-hand variable regulator: 50 hPa

Variable regulators:

3.1.2 Variable regulators

The variable regulators are set so that when they are turned slowly, the
values are changed by 1 hPa / 0.1 second per notch (0.01 – 0.02 psi).
If the regulators are turned faster, the values are altered in larger
increments.

13
3 Mode of operation

3.1.3 Keypad

Inject AUTO - INJECT n= 0

Performs automatic or manual injection.
Automatic injection is controlled via the 0.4 s
injection time ti, and the injection
pressure pi is used. In the second line, pi[hPa] ti[s] pc[hPa]
injection time ti is counted down from the 150 0.6 50
nominal value.

Manual injection is performed for as long as the key is held down.

Injection pressure pi is used. In the second line, injection time ti is counted
upwards from 0.0.
If the FemtoJet is connected to the Eppendorf Micromanipulator 5171 or
Inject Man, the injection movement of the micromanipulator is also
started via the key. Both units then combine as a system to perform
semi-automatic microinjection.
Alternatively, the INJECT key of the connected micromanipulator or the
hand/foot control can be used.
If synchronization is set to "limit" (SYNC = LIMIT) in the INJECT function
of the micromanipulator, semi-automatic injection can only be triggered
via the micromanipulator.

Clean CLEAN n= 0
The maximum available pressure for
cleaning is applied at the tube outlet for ≡≡≡≡≡≡≡≡≡≡≡≡≡≡≡_____
as long as the key is held down. Once the
key is released, the pressure drops to pi[hPa] ti[s] pc[hPa]
compensation pressure pc again. The 150 0.2 50
pressure curve is shown in the form of
a bar.

14
3 Mode of operation

Auto / Manu MANU - INJECT n= 0

To switch between manual and
automatic, time-controlled injection. 0.4 s
In the case of manual injection, time
pi[hPa] pc[hPa]
is counted upwards from 0.0 seconds. 150 50
The parameter ti is not displayed in the
bottom two lines in order to emphasize
that the time cannot be set via the
variable regulators in this case.

Count
To reset to zero the counter for injections
which have been actuated.

Menu
To switch the devict to menu control;
selected functions may be executed.

In the first line, FUNC indicates that the

FUNC 1
device is in the function menu and shows Select pressure unit
which function number is active. INJECT = PSI [hPa]
The second line contains the function
description (e.g. "Select pressure unit"). Turn pi to
select function
The third line requests an action (e.g.
press the key to change the
pressure unit). Current status: [hPa]
The two bottom lines show information
about which action is assigned to which
variable regulator (e.g. other functions
can be selected using left-hand variable
regulator pi).

15
3 Mode of operation

Standby
in the case of a brief interruption STANDBY
When key is pressed briefly:
The device switches to the Standby
mode without deairing. The illumination
Supply pressure 6789
of the display switches off. "STANDBY"
appears in the display with the suffix
"Supply pressure = rxyz" as a code for
system pressure ps being maintained.
Application:
For temporary interruptions or use of
as a Reset function.
When key is pressed again:
The device is reactivated and runs
through a short warm-up routine.
This lasts approximately one minute.

Standby
with deairing of pressure accumulator
Hold down the key (approx. 2 seconds; Hold standby
the display moves to "STANDBY"): to exhaust
The device switches to the Standby
mode. The pressure accumulator is
completely deaired, the illumination of
the display switches off. "STANDBY"
appears in the display with the note STANDBY
"Supply pressure = x" (x ~ 0).
A noise can be heard at this stage.
Application: Supply pressure 0
For correct shutdown, with deairing of the
entire pressure system in the event of
longer periods of non-use or standstill
(weekend or storage) with subsequent
switch-off using the mains switch.
Press the key again:
The device is reactivated and runs
through the warm-up routine.
This takes approximately three minutes.

16
3 Mode of operation

Reset
To reset the device to the initial mode by
pressing the key briefly or via the
mains switch.
The pressure accumulator is not deaired.

3.2 Hand control / foot control

The functions of the hand/foot control

correspond to the functions of the
and keys on the
FemtoJet.
The centre key of the hand control has
no function.

The function of the foot control

corresponds to the key.

17
3 Mode of operation

3.3 Connecting the capillary holder

Universal capillary holder
The universal capillary holder is used to locate the injection capillaries.
Prefabricated Eppendorf Femtotips / Femtotips II (6) are screwed into
the universal capillary holder using the adapter (5). To do so, the adapter
for Femtotips (5) is first screwed into the front rotatable knurled screw
(4) and then the Femtotip / Femtotip II is attached. If self-pulled
capillaries are to be attached, the grip head 0 (7) needs to be screwed
loosely into the front rotatable knurled screw (4). The self-pulled capillary
is pushed into the front opening of the grip head through the two O-rings
and fixed in position by tightening the grip head.

1 2 3 4 5 6

Universal capillary holder

1 Tube connection
2 Rear knurled screw
3 Tension piece
4 Front knurled screw, rotatable
5 Adapter for Femtotips (microcapillaries for microinjection)
6 Femtotip / Femtotip II
7 Grip head 0, plastic, with two O-rings and sealing washer

Connecting the universal capillary holder

The two-meter-long pressure tube is connected to FemtoJet (1) via the
bayonet joint (2). The pressure tube (3) is screwed to the rear knurled
screw (4) of the universal capillary holder. Femtotips / Femtotips II are
fitted at the front end of the universal capillary holder (5) or independently-
manufactured capillaries are clamped with the aid of the appropriate grip
head.

1 2 3 4 5

18
3 Mode of operation

Other accessories
Clamping piece
for fastening the universal capillary holder.
Not required for TransferMan NK or for Eppendorf
micromanipulators, which have an adjustable
cannula holder.

The universal capillary holder is mounted to the

micromanipulator by placing the clamping piece into
the tool holder of the Eppendorf Micromanipulator
and then tightening it with the fastening screw.
The universal capillary holder is positioned with
the correct length into the tool holder and then fixed
into position. The position of the capillary can be
changed by loosening the fastening screw.

Grip head 0,
incl. 2 sets of O-rings and sealing washer.
Using grip head 0 supplied, capillaries with an outer
diameter of 1.0 mm to 1.1 mm can be used. In the
case of outer diameters which differ from these
measurements, the relevant grip heads can be
ordered as non-standard accessories for the
universal capillary holder (see Section 7, Ordering
information). The transparent grip head allows the
user to visually monitor the position of the capillary.

19
3 Mode of operation

3.4 Information on working practices

The following pressures can be used:

Name Pressure range Comments

Compensation 0 – 6,000 hPa

pressure pc 0.00 – 87.0 psi

Injection pressure pi 0 – 6,000 hPa

0.00 – 87.0 psi

Clean pressure maximum

up to 7,000 hPa
maximum up to 102 psi

Constant working 0 – 6,000 hPa Select Function 4 via

pressure pw 0.00 – 87.0 psi the key.

System pressure ps maximum This is used directly

[supply pressure] up to 7,000 hPa for .
maximum up to 102 psi
In certain phases of
initialization, the
system pressure may
temporarily increase
to a maximum of
8,000 hPa (117 psi).

20
3 Mode of operation

3.4.1 Compensation pressure pc

The compensation pressure pc ensures that no medium flows into the
capillary. Capillary forces would make liquid flow out of the cell culture
dish into the injection capillary and thus dilute the injection material. To
prevent this, a permanent compensation pressure pc is set. This should
be selected so that there is a permanent slight flow-out of liquid from the
injection capillary. The individual pressure level can be determined in a
preliminary test. For example, a fluorescent dye can be added to the
capillary to enable outflow to be monitored directly. The pressure required
is heavily dependent on the surface tension and viscosity of the injection
material. Experience has shown that compensation pressures of between
30 hPa and 300 hPa (0.44 – 4.4 psi) are appropriate values for ready-to-
use Eppendorf Femtotips injection capillaries.

Compensation pressure pc
Capillary suction

Hydrostatic pressure P hyd

Pressure in the capillaries

3.4.2 Injection pressure pi

Injection pressure pi is applied during the injection process. This pressure
is generally higher than the compensation pressure. In many cases, a
pressure between 50 hPa and 500 hPa (0.73 – 7.2 psi) is appropriate for
Femtotips. The volume injected is dependent on the injection pressure
and the injection time. The injection pressure must be greater than
the internal pressure of the cell into which injection should take place.
However, if the cell or the cell nucleus swell up during injection, the
injection pressure is too high.
If the compressor starts up occasionally during experiments, this does not
impair the function of the FemtoJet. Pressure is always available up to
the maximum value of 6,000 hPa (approx. 87.0 psi), even when the
compressor is topping up the supply pressure.

21
3 Mode of operation

3.4.3 Injection time ti

Injection time ti gives the period for which injection pressure pi is
maintained. ti is usually selected from a range between approx. 0.3 s and
1.5 s. The relevant factor for the injected volume is the product of the
injection pressure and the injection time.
Depending on the setting on the Eppendorf Micromanipulator which is
connected, the injection time ti is measured from the point at which the
key is pressed. The injection pressure is built up immediately, so it
is already present during the injection movement and during penetration
of the cell. Once the end of injection time has been reached, the device
switches to compensation pressure pc. At the same time, the micromani-
pulator is moved back into the working level. When "Injection at Z limit" is
set, measurement of the injection time ti does not begin until the Z limit
has been reached. Once ti has elapsed, the FemtoJet switches back to
pc and the micromanipulator moves back into its starting position in the
working level.

3.4.4 Clean function

The Clean function is used to flush out blocked capillaries. The maximum
available pressure is applied to the outlet with the key. It is applied
for as long as the key is pressed. The maximum pressure of the
FemtoJet is in the range of 7,000 hPa (102 psi). The value cannot be
changed.
The pressure curve up to maximum pressure appears in the display in the
form of a bar chart. By pressing the key briefly, a lower pressure
can also be obtained.

22
3 Mode of operation

3.4.5 Manual or automatic injection

In the Manu operating mode, injection is performed for as long as the
key or hand / foot control is held down. The duration (ti) of
injection is measured and shown in the display. In the case of
combination with an Eppendorf Micromanipulator, the injection movement
is triggered.
In the Auto operating mode, the built-in timer facilitates a reproducible
injection time. The injection runs automatically for the duration of set time
ti. It is triggered by the key or the hand / foot control being pressed
briefly. During injection, the Inject key is inactive, i.e. pressing it again has
no effect. In combination with an Eppendorf Micromanipulator 5171 /
InjectMan, the automatic injection procedure can also be triggered from
the operating panel of the micromanipulator.

3.4.6 Constant pressure/constant working pressure

Using the Constant Pressure function, it is possible to select a higher
pressure permanently if required. This function is helpful when special
injection techniques require either a high pressure up to a maximum of
6,000 hPa (87 psi) or a permanent flow of injection solution
(see Section 3.7.5). Control accuracy drops in the pressure range from
2,000 – 6,000 hPa (29 – 87 psi).

3.4.7 General note

FemtoJet is fitted with an integrated compressor. For this reason, the
compressor may start up or excess pressure may be let off briefly before,
during and after injections or during periods when the FemtoJet is not
being used. This is not a malfunction of the device and does not impair
injection.
In the case of injections under high pressure at very short intervals
or in the case of the Clean function being carried out several times
in succession, the pressure in the system accumulator may drop
significantly. This is indicated by a warning or an error message. In this
case, wait a short time until the supply pressure has stabilized again.
It is then possible to continue working immediately.

23
3 Mode of operation

3.5 The first injection

The following are available:
● Adherent cells in a Petri dish (possibly on CELLocates to observe
specific cells after injection),
● Injection liquid (purified, e.g. by centrifugation),
● Femtotips / Femtotips II; other capillaries as an alternative
● Microloader,
● 0.5 – 10 µl pipette to take the Microloader,
● A universal capillary holder on the micromanipulator which has been
preadjusted with a Femtotip.

Filling the Femtotips with the Microloader:

– Select function 0 "Capillary exchange" by pressing . Connect
the pressure tube.
– Aspirate approximately 0.5 – 5.0 µl of centrifuged injection fluid with
the Microloader without touching the base of the vessel. Using the
Microloader, fill the Femtotip through the rear opening, inserting the
tip approximately up to the centre of the Femtotips and releasing the
fluid there.
– Hold the Femtotip vertically, loosen the cap by turning it and allow it
to drop vertically.
– Screw the Femtotip into the capillary holder.
– Complete Function 0 by pressing . Compensation pressure is
applied.
– Move the capillary into the cell medium (Petri dish) with the micro-
manipulator.
– Focus the cells with the microscope, position the capillary slightly
above the cells but do not allow it to come into contact with the base
of the Petri dish. Focus the capillary.
– Using the key, check that the capillary is not blocked. Either
the fluid can be seen flowing out or it is rendered visible by the
fluorescent substance it entrains. As a rule, any air bubbles present
are blown out.
– Perform a sample injection using the preset parameters
(e.g. pi ~ 150 hPa, ti ~ 0.5 s, pc ~ 50 hPa, corresponding to
pi ~ 2.18 psi, ti ~ 0.5 s, pc ~ 0.73 psi). Injection can be monitored
visually when the cell size changes by approx. 5 % to 10 % or via
fluorescence. If required, adapt parameters pi and/or ti.

24
3 Mode of operation

3.6 Connecting an Eppendorf Micromanipulator

Connection to an Eppendorf Micromanipulator designed for automated
injection is effected with the 15-pin interface.

1 3 4 5

2 7 6

FemtoJet (rear) with all connection options

1 Foot control (optional) with Inject function

2 Hand control with Inject and Clean functions
3 FemtoJet
4 Micromanipulator 5171 or InjectMan
5 Connection socket of micromanipulator for
Transmission cable 5246 ("Y cable")
6 Transmission cable 5246
(accessory for Micromanipulator 5171 and InjectMan)
7 Interface cable 5171
(Connection from FemtoJet to Transmission cable 5246 "Y cable")
8 Interface of FemtoJet to micromanipulator

25
3 Mode of operation

3.7 Functions
The Function menu is called up using the key. One of seven
functions is selected using left-hand variable regulator pi. The relevant
functionality is assigned to the variable regulators in the bottom line of the
display.
Exit the Function menu by pressing the key again.
In cases of error, pressure control is switched off from within the Function
menu. Then exit the Menu using the key.
Function 6, self-test, is described in Section 5, "Troubleshooting".

3.7.1 Changing the capillaries

FUNC 0
FUNC 0
Change capillary When this function is called up, the
Capillary may be pressure tube is deaired and any
changed now pressure present in the system is let out.
Turn pi to Once the capillary has been changed,
select function exit the function using the key or
variable regulator pi; pressure control will
restart.

3.7.2 Setting the pressure unit

FUNC 1
FUNC 1
Push Inject to select The other pressure unit is selected using
Pressure Unit hPa PSI the key and all pressure values
are converted. The pressure unit
Turn pi to
currently selected (in this case: hPa)
select function
is underlined.

26
3 Mode of operation

3.7.3 Switching on/off the loudspeaker

FUNC 2
FUNC 2
Push Inject to select Use the key to switch the
Beeper ON OFF loudspeaker on or off. The currently
selected setting (in this case: ON) is
Turn pi to underlined. Acoustic error messages are
select function not emitted if the setting
is OFF.

3.7.4 Switching on/off the background illumination of the display

FUNC 3
FUNC 3
Push Inject to select Use the key to switch the
Illumination ON OFF illumination on or off. The currently
selected setting (in this case: ON) is
Turn pi to underlined.
select function
In the normal operating mode,
illumination switches off after three
minutes if no operating control has been
actuated. When an operating control is
actuated, the illumination lights up again.

3.7.5 Constant pressure

FUNC 4
FUNC 4
Continuous flow This function can be used to apply a
INJECT = start constant working pressure pw in the
range from approx. 0 to 6,000 hPa
Turn pi to (87.0 psi) at the pressure outlet. The
select function pressure unit psi can also be used.

The working pressure pw is set with

FUNC 4
Continuous flow the right-hand variable regulator (pc),
pw= 50 hPa the specified value is shown in large
characters. The actual value is shown
Turn pi to pw[hPa] in the bottom right of the display.
select function 50
The Clean function can be activated. The
pressure of the Clean function is shown
in the bottom right of the display in the
form of pw.

27
3 Mode of operation

3.7.6 Dewatering the pressure accumulator

FUNC 5
FUNC 5
Drain pressure supply – Briefly opens the dewatering valve
INJECT = start of the pressure accumulator; any
condensed humidity present is blown
Turn pi to away.
select function
– When this function is performed, the
noise of air being blown away can be
heard.
This function is automatically performed
every time the unit is switched on. It can
be performed manually as described if
condensation is suspected to be present.

28
4 Care and maintenance

4.1 Cleaning
Before cleaning the FemtoJet, disconnect the plug.
Ensure that no fluids enter into the inside of the injector in order to avoid
short-circuits in the electrical installation as well as corrosion.
Wipe painted parts and aluminum surfaces using a cloth and mild
detergent and then with a dry cloth.
Warning: Do not use any corrosive, solvent or abrasive detergents
or polishes.

4.2 Disinfection
Clean the FemtoJet before disinfecting.
Wipe every part of the FemtoJet, including accessories and connector
cables, with disinfectant.
Spray disinfection is not advisable, as disinfectants can enter into the
microinjector.
The disinfection method used must comply with current disinfection
regulations and guidelines.

4.3 Maintenance
Regular maintenance is not required.
The dealer is responsible for warranty and service.

29
5 Troubleshooting

5.1 Potential errors

– Pressure loss due to capillary not fitted with capillary holder
connected.
Solution: Connect the capillary or select Function 0, Change capillary,
via the key and variable regulator pi.
– Capillary is blocked.
Solution: Press the key for long enough.
Change capillary.
Purify injection fluid (e.g. by means of centrifugation).
– Compressor runs for a long time.
Solution: Take a short break.
Do not use the key too often within a short period.
If possible, set a lower compensation pressure pc.
Perform self-test (Section 5.1.1).
– Valve rattles
Solution: Connect tube to filled capillary. Pressure control is designed
for the tube connected.
– No communication with the micromanipulator.
Solution: Check cables and connections.

5.1.1 Self-test

FUNC 6
FUNC 6
Self-Test The FemtoJet is checked for leaks in
INJECT = Complete the self-test.
CLEAN = Leak only
Connect closed tube !
This self-test can also be used if a leak is
Turn pi to suspected.
select function
The test runs automatically.
The pressure tube must be connected to
a closed capillary (e.g. melted closed or
filled) for the pressure control to work
properly.

30
5 Troubleshooting

FUNC 6
key: Complete self-test
Self-Test
Compressor _____ s – Checks compressor run time up to
Ps leak _____ _____ hPa the point when a specific pressure
Pw leak _____ _____ hPa
Injection _____ _____ hPa is reached and thus for leaks in the
area of the compressor.
ps=7000 pw= 10 hPa
– In the case of two different
pressures, checks for leaks in the
pressure accumulator (ps) and for
the internal tightness of the valves.
– Checks for leaks in the area of
working pressure (pw) in the case
of two different pressures.
– Checks behavior during injection
at different pressures (short
continuous test).
If certain values are outside the specified
range, an error message is emitted. If this
recurs, inform Service.

FUNC 6
key: Self-test to search for leaks
Self-Test
– Checks for leaks in the pressure
Ps leak _____ _____ hPa accumulator (ps) in the case of two
Pw leak _____ _____ hPa
different pressures, as well as for
inner tightness of the valves.
ps=7000 pw= 10 hPa
– Checks for leaks in the area of wor-
king pressure (pw) in the case of two
different pressures.
If certain values are outside the specified
range, an error message is emitted. If this
recurs, inform Service.

31
5 Troubleshooting

5.2 Warnings
As a rule, warnings are emitted if certain operating conditions are not (yet)
reached or if an action is not possible.
Acknowledge the warning with the key and the unit will return to
normal status.

WARNING 001
Warning 1
Supply pressure has not yet been
Please wait for reached.
supply pressure

INJECT = Continue The minimum supply pressure required

for an injection or the Clean function was
not present when the function was
triggered.
Possible cause:
Multiple triggering of Clean or very rapid
injection at a high injection pressure.
Solution:
Wait until the supply pressure has built up
and trigger again.

WARNING 002
Warning 2
Compensation pressure cannot be
Check capillary maintained.
e.g. broken/missing ?
Possible cause:
Capillary or capillary holder not attached
INJECT = Continue or only connected loosely.
Solution:
Check all connections and tighten.
Activate Function 0 to change the
capillary and when the capillary holder is
open.

32
5 Troubleshooting

WARNING 003
Warning 3
Micromanipulator does not react
Switch on manipulator
or disconnect cable A micromanipulator is connected but
INJECT = Continue does not react to a start pulse from the
FemtoJet.
Possible cause:
Micromanipulator is switched off.
INJECT function on manipulator is not
released.
No limit is set on the micromanipulator.
In the INJECT function on the micro-
manipulator, synchronization is set to
limit (SYNC = LIMIT).
Solution:
Switch on the micromanipulator and
make it operational.
Disconnect the cable to micro-
manipulator.
With SYNC = LIMIT, trigger semi-auto-
matic injection via the micromanipulator
only.

5.3 Error messages

Error messages are emitted if certain operating conditions are not
reached or the device records a deviation from specified conditions.
Acknowledge the error message with the key and the unit will
return to normal status. The measures described under “Solution” should
be carried out. If the error message recurs after acknowledgement with
the key, inform Service.

33
5 Troubleshooting

ERROR 010
Error 10
Supply pressure cannot be reached
Supply pressure
cannot be reached The compressor runs without the
INJECT = Continue maximum supply pressure being
reached.
Possible cause:
High compensation pressure when the
capillary holder is not attached or is
poorly attached.
Technical cause:
Compressor does not start up. Leak in
compressor or pressure accumulator.
Solution:
Check the capillary holder and tube for
leaks or tighten up the tube connections.
Service.

ERROR 011
Error 11
Supply pressure is too high
Supply pressure is
too high Supply pressure has exceeded the
INJECT = Continue maximum permissible supply pressure.
Possible cause:
supply pressure is not relieved by the
relevant standby function.
Technical defect.
Solution:
Switch off using the key (hold
down for approximately two seconds).
Service.

ERROR 012
Error 12
Supply pressure does not drop far
Supply pressure is enough
does not fall

INJECT = Continue Possible cause: Technical defect

Solution: Service

34
5 Troubleshooting

ERROR 013
Error 13
Supply pressure does not rise
Supply pressure is
does not raise Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 014
Error 14
Massive leak in pressure accumulator
Supply pressure
leak is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 015
Error 15
Leak in pressure accumulator
Supply pressure
leak is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 016
Error 16
Leak through Valve V1 is too large
Valve 1 pressure
leak is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 020
Error 20
Working pressure has not been reached
Working pressure
cannot be reached Compressor runs without supply
INJECT = Continue pressure being reached within a specific
time.
Possible cause:
Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check the capillary. Check the capillary
holder and tube for leaks or tighten up the
tube connections.
Service.

35
5 Troubleshooting

ERROR 021
Error 21
Injection pressure has not been reached
Inject pressure
cannot be reached Possible cause:
INJECT = Continue Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

ERROR 022
Error 22
Compensation pressure has not been
Compensation pressure reached
cannot be reached

INJECT = Continue Possible cause:

Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

ERROR 023
Error 23
Increase in working pressure is smaller
Inject n pressure than required
overflow too large

INJECT = Continue Possible cause: Technical defect

Solution: Service

ERROR 024
Error 24
No increase in working pressure
Working pressure
does not rise Possible cause: Technical defect
INJECT = Continue Solution: Service

36
5 Troubleshooting

ERROR 025
Error 25
No drop in working pressure
Working pressure
does not fall Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 026
Error 26
Working pressure leak rate is too high
Working pressure
leak is too high Possible cause:
INJECT = Continue Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

ERROR 027
Error 27
Working pressure leak rate is too high
Working pressure
leak is too high Possible cause:
INJECT = Continue Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

37
5 Troubleshooting

ERROR 028
Error 28
Working pressure leak rate is too high
Working pressure
leak is too high Possible cause:
INJECT = Continue Capillary holder not attached or poorly
attached. (Broken) capillary with large
opening.
Technical defect.
Solution:
Check capillary. Check capillary holder
and tube for leaks or tighten up tube
connections.
Service.

ERROR 030
Error 30
Sensor offset supply pressure is too low
Sensoroffset supply
is too low Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 031
Error 31
Sensor offset supply pressure is too high
Sensoroffset supply
is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 032
Error 32
Sensor offset working pressure is too low
Sensoroffset work
is too low Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 033
Error 33
Sensor offset working pressure is too
Sensoroffset work high
is too high

INJECT = Continue Possible cause: Technical defect

Solution: Service

ERROR 034
Error 34
Valve 1 offset is too low
Valve 1 offset
is too low Possible cause: Technical defect
INJECT = Continue Solution: Service

38
5 Troubleshooting

ERROR 035
Error 35
Valve 1 offset is too high
Valve 1 offset
is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 036
Error 36
Valve 2 offset is too low
Valve 2 offset
is too low Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 037
Error 37
Valve 2 offset is too high
Valve 2 offset
is too high Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 040
Error 40
Self-calibration is missing
Self-Calibration
is missing Possible cause: Technical defect
INJECT = Continue Solution: Service

ERROR 041
Error 41
Full-scale calibration is missing
Fullscale Calibration
is missing Possible cause: Technical defect
INJECT = Continue Solution: Service

39
6 Technical data

Voltage/Frequency: 100 V – 240 V ± 10 %, 50 – 60 Hz

Set voltage: adapts automatically
Fuse for 100 – 240 V: T1.0 A, 250 V (2 pcs.)
Power consumption: 50 W
Max. current consumption: < 0.5 A
Protection class: I
Overvoltage category: II (IEC 61010-1)
Pollution degree: 2 (IEC 664)
Ambient temperature: 15 °C – 35 °C
Ambient relative humidity:max. 70 %
Weight: 6.2 kg
Dimensions: Width: 220 mm
Depth: 170 mm
Height: 280 mm
Injection time ti: 0.0 – 99.9 s
in 0.1 s increments
Injection pressure pi: 0 – 6,000 hPa (87 psi),
in 1 hPa (0.01–0.02 psi) increments
Regulating accuracy: 1 % of value set in each case,
at least ± 1 hPa
Compensation pressure pc: 0 – 6,000 hPa (87 psi),
in 1 hPa (0.01–0.02 psi) increments
Regulating accuracy: 1 % of value set in each case,
at least ± 1 hPa
Clean rinsing pressure: max. approx. 7,000 hPa (102 psi)
RS 232 computer Baud rate: 9,600
interface: Start bit: 1
Data bits: 8
Parity: non
Stop bit: 1

The device is approved with the sign.

Technical specifications subject to change!

40
7 Ordering information

Order no.
5247 000.013 FemtoJet
100 V – 240 V ± 10 %, 50 – 60 Hz
Fully programmable microinjector with integrated
pressure supply. For injection of smallest amounts
of liquid into living cells.
Delivery package:
1 FemtoJet
1 Power cable
1 Interface cable 5171
1 Universal capillary holder
1 Pressure tube, 2 m
5247 617.002 1 Hand control for remote control
10 Femtotips / Femtotips II (injection capillaries)
10 Microloader
20 CELLocates
1 Operating Manual

Accessories (not standard)

5247 623.002 Foot control FemtoJet
5325 620.007 Interface cable PC
for control and for program updates
5247 622.006 Interface cable 5171
for connection to the micromanipulator
5242 952.008 20 Femtotips
5242 957.000 20 Femtotips II
5242 956.003 200 Microloader
5246 164.004 1 Pressure tube, 2 m, for universal capillary holder
5176 190.002 Universal capillary holder
(incl. grip head 0, adapter for Femtotips,
clamping piece and spare O-rings)
4910 000.018 Eppendorf Reference pipette, variable, 0.5 to 10 µl
3110 000.021 Eppendorf Research pipette, variable, 0.5 to 10 µl
5176 210.003 Grip head 0 for universal capillary holder;
for microcapillaries with an outer diameter
of 1.0 to 1.1 mm
5176 212.006 Grip head 1 for universal capillary holder;
for microcapillaries with an outer diameter
of 1.2 to 1.3 mm
5176 214.009 Grip head 2 for universal capillary holder;
for microcapillaries with an outer diameter
of 1.4 to 1.5 mm

41
7 Ordering information

5176 207.002 Grip head 3 for universal capillary holder

for microcapillaries with an outer diameter
of 0.7 to 0.8 mm
5176 196.000 O-ring set
for grip heads 0 to 3 with extraction tool
5176 195.004 Service kit
for grip head and capillary holder,
consisting of O-ring set, adapter for Femtotip
and clamping piece

CELLocate
Glass coverslips with integrated microgrid
for relocating cells in light and electron microscopy,
individually sealed, with log pad
5245 952.009 Grid size 175 µm, sterile, set of 80
5245 962.004 Grid size 55 µm, sterile, set of 80

Micromanipulation
5171 000.019 Micromanipulator 5171
Programmable micromanipulator with
coordinate storage for automatic microinjection,
microdissection and electrophysiology
(in combination with FemtoJet),
complete with power unit, module unit
and control board
5179 000.018 InjectMan
Micromanipulator for automatic injection
in adherent cells in combination with FemtoJet,
complete with power unit, module unit
and control board
5178 000.014 TransferMan NK
Proportional micromanipulator for
microinjection in suspension cells
5177 000.010 TransferMan
Dynamic micromanipulator for
microinjection in suspension cells
5246 621.006 Transmission cable 5246
"Y cable" for connection with the
interface cable 5171
5211 058.007 Foot control for microinjection,
connectable to transmission cable 5246 "Y cable"

42
8 Index

A G
Adapter for Femtotips 18 Grip head 18, 19
Application 3, 4
H
C Hand control 17
Capillary change 26
I
Clean 8
Information on working practices 20
pressure 20
Injection
function 22
automatic 14, 23
Cleaning 29
first 24
Commands 45
manual 14, 23
Compressor 21, 23
movement 14, 23
Compensation pressure pc 5, 20, 21
parameters 12
Connection
pressure pi 5, 14, 20, 21
capillary holder 18
time ti 5, 14, 22
micromanipulator 25
trial 24
universal capillary holder 18
Interface 10, 25
Constant
description 45
pressure 23, 27
working pressure 23 K
working pressure pw 20 Key
Auto/Manu 8, 14
D
Clean 8, 14, 22
Disinfection 29
Count 8, 15
Display 6, 8, 12
Inject 5, 8, 14, 32
in hPa 12
Menu 8, 26
in psi 12
Standby 8, 16
switch on/off 27
Keypad 6, 14
E
L
Error
Loudspeaker, switch on/off
messages 33
potential 30 M
Mains switch 10
F
Menu 15
Foot control 7, 17
Mode of operation 12
Functions 26
keys 8 O
menu 15 Operation 12
via computer 43, 45
Ordering information 41
Overview 6

43
8 Index

P
Pressure
– dewatering
pressure accumulator 28
– set pressure unit 26
tube 9, 18
Q
Query 45
R
Reset 9, 17
function 16
S
Safety precautions 3, 4
Self-test 30
complete 31
to find leaks 31
Servicing 29
Short description 8
Startup 10
Standby without deairing 16
Switch
on 10
off 11
System pressure ps 20
T
Technical data 40
Tension piece 19
Troubleshooting 30
Tube connection 6, 9
U
Unit
description 5
rear of 10
Universal capillary holder 18
V
Variable regulator 5, 6, 9, 13, 26
W
Warnings 32

44
Appendix A

Operation via a computer

Certain commands and queries (Section 6.2) can be transmitted to the
unit using the optional cable/PC interface.

A.1 Description of interface

A computer can be connected to the remote control of the FemtoJet via
one of the two RS 232 interfaces. Parameters can be entered or read out
via the computer.
The computer is connected by the special cable/PC interface cable.
The computer to be connected must comply with EN 60950/UL 1950
standards.
The serial interface of the computer should be set to the following trans-
mission parameters:
Baud rate: 9,600
Start bit: 1
Dat bits: 8
Parity: non
Stop bit: 1
Only ASCII symbols (incl. CR/LF) which can be represented are
transmitted.Therefore a terminal program, for example, is suitable for
remote control.

A.2 Commands and queries

The commands to be sent are defined with the following structure:
Commands to enter parameters: "Cxxx=ssssssssss"
Commands to execute and to read out parameters: "Cxxx"
C: Command
xxx: Code for the executing command
=: Separation for the following input parameters
ssssssssss: Input parameters

In response to any command, the device provides a symbol string of a

similar structure:
"Axxx" for answers without output parameter
"Axxx=rrrrrrrrrr" for responses with output parameter
A: Answer

45
Appendix A

If the command is faulty, an error message is returned instead of the

answer:
"ERR=eee"
ERR: Error
=: Separation
eee: Error number
Lower and upper case letters can be used.

Command to enter Code Input Meaning of

parameters parameter input parameters
[C] [xxx] [ssssssssss]
Short acoustic signal 003 1 – 254 Number of signals

Long acoustic signal 004 1 – 254 Number of signals

Automatic injection 010 0; 1 – 999 Injection time

(inject at ti) in 1/10 seconds
0: injection time set on
the FemtoJet

Set injection counter n 012 ––– No parameter input

to 0

Perform Clean 013 1 – 100 Time in 1/10 seconds

Execute Standby 014 0 or 1 0 = short

1 = long

Switch acoustic signal 040 0 or 1 Status:

on/off 0 = off / 1 = on

Set pressure unit 041 0 or 1 Unit:

0 = hPa / 1 = psi

Set injection time ti 042 1 – 999 Injection time

in 1/10 seconds

Set compensation 043 0 – 6,000 / 87 Pressure in hPa/psi

pressure pc

Set injection pressure pi 044 0 – 6,000 / 87 Pressure in hPa/psi

46
Appendix A

Command to enter Code Input Meaning of

parameters parameter input parameter
[C] [xxx] [ssssssssss]
Set background 045 0 or 1 0 = off
illumination 1 = on

Dewater 046 ––– No parameter input

Command to read out Code Significance of Output

parameters output parameter parameter
[C] [xxx] [A] [rrrrrrrrrr]
Query program version 101 Versions number x.xx

Query bleeper 150 Status: 0 or 1

0 = off / 1 = on

Query pressure unit 151 Unit: 0 or 1

0 = hPa / 1 = psi

Query injection time ti 152 Injection time 1 – 999

in 1/10 seconds

Query compensation 153 Pressure in hPa/psi 0 – 6,000 / 87

pressure pc
Query injection 154 Pressure in hPa/psi 0 – 6,000 / 87
pressure pi

Query background 155 Status: 0 or 1

illumination 0 = off / 1 = on

47
Appendix A

Error no. Cause Solution

for incorrect
operating
construction
ERR = [eee]
060 The first character is not Check entry parameters.
a c or C. Change entry.

061 The 2nd to 4th character Check entry parameters.

is not a digit. Change entry.

062 The 5th character is not =. Check entry parameters.

Change entry.

063 The parameter is not Check entry parameters.

within the permitted range. Change entry.

064 The command is not Check entry parameters.

known. Change entry.

48
Appendix A

Error no. Cause Solution

during
execution
ERR = [eee]
070 FemtoJet is not in routine Check FemtoJet.
mode. Bring FemtoJet into
routine operation.
Eliminate error.

071 FemtoJet is not ready. Check FemtoJet or

micromanipulator.
As necessary, wait or
eliminate error.

072 The command is not finished Check FemtoJet or

within the scheduled time. micromanipulator.
Eliminate error.

073 This command cannot be Check FemtoJet.

executed at the moment, Eliminate error.
because of insufficient storage
pressure.
See: Warning 001

074 The connected micro- Check micro-

manipulator did not respond. manipulator.
See: Warning 003 Eliminate error.

Other error numbers as well as the error cause and solution are described
in Section 5, "Troubleshooting". During remote control, no error texts
appear in the FemtoJet display.

49
 Support and Services Directory
Contact Information

United States Canada

Business Hours: 8:30 a.m. to 6:00 p.m. EST 8:30 a.m. to 6:00 p.m. EST

Phone: 800-645-3050 800-263-8715

516-334-7500 905-826-5525

Fax: 516-334-7506 905-826-5424

Address: Eppendorf North America, Inc. Brinkmann Instruments (Canada) Ltd.

One Cantiague Road 6670 Campobello Road
Westbury, NY 11590-0207 Mississauga, ONT L5N 2L8

Website: www.eppendorf.com www.brinkmann.com

Email: info@eppendorf.com canada@brinkmann.com

Customer 800-645-3050, menu option 2 800-263-8715, menu option 1

Support: custserv@eppendorf.com custserv@brinkmann.com

© 2005 Eppendorf AG. Eppendorf® is a registered trademark of Eppendorf AG. Add other trademark information here.
Repair: 800-645-3050, ext. 2404 800-263-8715, ext. 232
service@eppendorf.com service@brinkmann.com

Product appearance, specifications, and/or prices are subject to change without notice.
Applications 800-645-3050, ext. 2258 800-645-3050, ext. 2258 (U.S.)
Lab: apps@eppendorf.com bioapps@brinkmann.com

For more information contact your Eppendorf North America Sales Representative at 800-645-3050.

www.eppendorf.com • Email: info@eppendorf.com • Application hotline: 516-515-2258

In the U.S.: Eppendorf North America, Inc. 800-645-3050 • In Canada: Brinkmann Instruments (Canada) Ltd. 800-263-8715