Week 12- Assignment 12 (10 Qs in MSQ format)
Select the best one:
1. Which of the following is not a commonly used bioinformatics tool for understanding
biological data?
a. MEGAN
b. Adobe Photoshop
c. QIIME
d. BLAST
Answer 1. (b) Adobe Photoshop
2. How does Sequencing depth affect the accuracy of a sequencing run?
a. Accuracy increases with the increase of sequence depth.
b. Accuracy decreases with the increase of sequence depth.
c. There is no relationship between sequencing depth and accuracy.
d. Accuracy increases initially with the increase of sequence depth but eventually
reaches the plateau.
Answer 2. (d) Accuracy increases initially with the increase of sequence depth but eventually
reaches the plateau.
3. What is the advantage of using a Microarray or multiplexed PCRs over the traditional
PCR methods for detecting microbial communities?
a. Microarrays and multiplexed PCRs are more sensitive than PCR-based methods.
b. Microarrays and multiplexed PCRs are speedier than PCR-based methods.
c. Microarrays and multiplexed PCRs can simultaneously detect higher number of
microbial taxa.
d. Microarrays are more economical than PCR-based methods.
Answer 3. (c) Microarrays and multiplexed PCRs can simultaneously detect higher
number of microbial taxa.
4. Which one of the following best describes genome assembly in bioinformatics?
a. Predicting the functions of genes based on their sequence similarity to known genes.
b. Determining the order and orientation of DNA fragments to reconstruct the
original sequence of a genome.
c. Aligning RNA sequences to a reference genome to determine differential gene
expression.
d. Identifying and characterizing mutations in a genome sequence.
Answer 4. (b) Determining the order and orientation of DNA fragments to reconstruct the
original sequence of a genome.
5. Statement A: BLAST is used in bioinformatics and molecular biology research to
identify homologous sequences and infer functional and evolutionary relationships
between them.
Statement B. SINA is optimized for aligning large datasets and provides high-quality
alignments suitable for downstream analysis, such as phylogenetic inference and
taxonomic classification.
a. Statement A is True, and B is False
b. Statement B is True, and A is False.
c. A and B are both true statements.
d. A and B are both false statements.
Answer 5. (c) A and B are both true statements.
6. What is the main advantage of high-throughput sequencing over traditional Sanger
sequencing?
a. Higher throupghput of sequencing
b. Ability to sequence longer reads.
c. Higher accuracy and longer reads.
d. Ability to sequence DNA and RNA simultaneously.
Answer 6. (c) Higher accuracy and longer reads.
7. _________is a publicly available database that stores DNA and RNA sequence data
and associated metadata, such as sequence annotations and literature references.
a. MacVector
b. GenBank
c. Adobegene
d. Qiime
Answer 7. (b) GenBank
8. ______________ is the study of proteins and their interactions with other molecules. It
uses various techniques, including mass spectrometry and protein microarrays, to
identify and characterize proteins and their functions.
a. Proteomics
b. Genomics
c. Bioinformatics
d. Cheminformatics
Answer 8. (a) Proteomics
9. What is the purpose of bioinformatic analysis in metagenomics?
a. To assemble the short DNA sequences generated by sequencing into longer contiguous
sequences.
b. To compare the DNA sequences in the metagenome to known sequences in databases
c. To identify and annotate the genes present in the metagenome.
d. To determine the functional potential of the microbial community represented by the
metagenome
e. All of the above
Answer 9. (e) All of the above
10. __________is used for taxonomic classification of microorganisms, present in
samples such as soil, water, and air.
a. 16S rRNA
b. Sanger Sequencing
c. High throughput sequencing
d. Long read sequencing.
Answer 10. (a) 16S rRNA
