"Ch#2 Enzyme Bio
MCQs ✏️✏️✏️
1_Enzymes are:
a) Carbohydrates
b) Lipids
c) Proteins
d) Nucleic acids
2_The structure of an enzyme is primarily composed of:
a) Peptide bonds
b) Disulfide bonds
c) Hydrogen bonds
d) All of the above
3_Enzymes act by:
a) Decreasing activation energy
b) Increasing activation energy
c) Changing substrate concentration
d) None of the above
4_The active site of an enzyme is:
a) Where substrates bind
b) Where products bind
c) Both a and b
d) None of the above
5_Enzymes function optimally at:
a) High pH and low temperature
b) Low pH and high temperature
c) Neutral pH and moderate temperature
d) Acidic pH and high temperature
6_Which of the following factors can affect enzyme activity?
a) pH
b) Temperature
c) Substrate concentration
d) All of the above
7_Non-competitive inhibition occurs when:
a) The inhibitor binds to the active site
b) The inhibitor binds to a site other than the active site
c) The substrate cannot bind to the enzyme
d) None of the above
8_Enzyme inhibition can be reversible or irreversible. Which of the following is reversible
inhibition?
a) Competitive inhibition
b) Non-competitive inhibition
c) Irreversible inhibition
d) Both a and b
9_Enzymes are classified into several classes based on:
a) Their substrate specificity
b) Their mode of action
c) Both a and b
d) None of the above
10_The energy of activation is:
a) The energy required to start a reaction
b) The energy released during a reaction
c) The energy stored in an enzyme
d) The energy required to bind substrates to enzymes
11_Which of the following is NOT a type of enzyme inhibition?
a) Competitive
b) Non-competitive
c) Uncompetitive
d) Linear
12_Enzymes are important because:
a) They speed up chemical reactions
b) They lower activation energy
c) They are specific in their action
d) All of the above
13_Enzymes can be classified based on the type of reaction they catalyze, such as:
a) Oxidoreductases
b) Hydrolases
c) Transferases
d) All of the above
14_Which of the following is NOT a characteristic of enzymes?
a) They are consumed in the reaction
b) They are highly specific
c) They are affected by temperature and pH
d) They can catalyze both forward and reverse reactions
15_Enzyme-substrate specificity refers to:
a) The ability of an enzyme to bind to multiple substrates
b) The ability of an enzyme to bind to one specific substrate or a group of structurally related
substrates
c) The ability of an enzyme to catalyze multiple reactions
d) The inability of an enzyme to bind to any substrate
16_Which of the following is a characteristic feature of enzyme inhibition?
a) It decreases the rate of enzyme-catalyzed reactions
b) It increases the rate of enzyme-catalyzed reactions
c) It does not affect the rate of enzyme-catalyzed reactions
d) It alters the structure of the enzyme
17_Enzymes that catalyze the transfer of functional groups from one molecule to another are
classified as:
a) Oxidoreductases
b) Transferases
c) Hydrolases
d) Lyases
18_Allosteric regulation of enzymes involves:
a) Binding of a regulatory molecule to the active site
b) Binding of a regulatory molecule to a site other than the active site
c) Changing the pH of the environment
d) Increasing the temperature of the reaction
19_The Km value of an enzyme-substrate reaction represents:
a) The substrate concentration at which the reaction rate is half of Vmax
b) The maximum velocity of the reaction
c) The rate constant of the enzyme-substrate complex formation
d) The pH at which the enzyme is most active
20_Enzymes are denatured at extreme:
a) pH and temperature
b) pH and pressure
c) Temperature and pressure
d) None of the above
21_Enzyme activation energy is:
a) The energy required to form an enzyme-substrate complex
b) The energy required to break bonds in the substrate
c) The energy released during the reaction
d) The energy required to activate the enzyme
22_Which of the following is an example of an irreversible enzyme inhibitor?
a) Competitive inhibitor
b) Non-competitive inhibitor
c) Allosteric inhibitor
d) Suicide inhibitor
23_The Michaelis-Menten equation describes the relationship between:
a) Enzyme activity and substrate concentration
b) Enzyme activity and enzyme concentration
c) Enzyme activity and product concentration
d) Enzyme activity and temperature
24_Enzyme cofactors are:
a) Organic molecules
b) Inorganic ions
c) Both a and b
d) Neither a nor b
25_Enzymes are named by adding the suffix "-ase" to the name of:
a) The substrate
b) The product
c) The cofactor
d) The enzyme class
26_Enzyme specificity refers to:
a) The ability of an enzyme to catalyze a particular reaction
b) The ability of an enzyme to bind to any substrate
c) The ability of an enzyme to catalyze multiple reactions
d) The inability of an enzyme to catalyze any reaction
27_Enzyme kinetics is the study of:
a) Enzyme structure
b) Enzyme function
c) Enzyme classification
d) Enzyme inhibition
28_Enzyme activity can be affected by:
a) pH
b) Temperature
c) Substrate
d) All of the above
29_Which of the following is NOT a type of enzyme inhibition?
a) Allosteric inhibition
b) Competitive inhibition
c) Uncompetitive inhibition
d) Feedback inhibition
30_Enzymes lower the activation energy of a reaction by:
a) Increasing the energy of the reactants
b) Decreasing the energy of the reactants
c) Increasing the energy of the products
d) Decreasing the energy of the
31_Which of the following is true about allosteric enzymes?
a) They have multiple active sites
b) They are not regulated by effector molecules
c) They undergo a conformational change upon binding of a regulatory molecule
d) They catalyze only irreversible reactions
32_The competitive inhibitor:
a) Binds to the active site of the enzyme
b) Binds to a site other than the active site of the enzyme
c) Increases the rate of the reaction
d) Does not affect the enzyme's activity
33_Enzymes are classified into six major classes based on:
a) Their substrate specificity
b) Their mode of action
c) Both a and b
d) None of the above
34_The turnover number (kcat) of an enzyme represents:
a) The maximum velocity of the reaction
b) The number of substrate molecules converted to product per unit time
c) The concentration of enzyme required for half-maximal activity
d) The rate constant of the enzyme-substrate complex formation
35_Enzymes catalyze reactions by:
a) Increasing the free energy change of the reaction
b) Decreasing the free energy change of the reaction
c) Increasing the activation energy of the reaction
d) Decreasing the activation energy of the reaction
36_Which of the following is an example of an enzyme cofactor?
a) ATP
b) H2O
c) NAD+
d) CO2
37_Enzyme inhibition is reversible when:
a) The inhibitor binds tightly to the enzyme
b) The inhibitor binds loosely to the enzyme
c) The inhibitor forms a covalent bond with the enzyme
d) The inhibitor binds non-specifically to the enzyme
38_Enzyme activity is typically measured by:
a) The change in substrate concentration over time
b) The change in product concentration over time
c) The change in enzyme concentration over time
d) The change in cofactor concentration over time
39_Which of the following is NOT a factor affecting enzyme activity?
a) pH
b) Temperature
c) Substrate concentration
d) Product concentration
40_Enzymes that catalyze the addition of water to a substrate are classified as:
a) Oxidoreductases
b) Hydrolases
c) Transferases
d) Lyases
41_Enzymes are specific because:
a) They can bind to any substrate
b) They can catalyze any reaction
c) They have unique active sites that fit specific substrates
d) They are not affected by changes in temperature or pH
42_The maximum velocity (Vmax) of an enzyme-catalyzed reaction is reached when:
a) All enzyme molecules are bound to substrate
b) All substrate molecules are converted to product
c) The enzyme concentration is zero
d) The substrate concentration is infinite
43_Enzymes are classified into subclasses based on:
a) Their substrate specificity
b) Their mechanism of action
c) Both a and b
d) None of the above
44_Enzymes increase the rate of chemical reactions by:
a) Increasing the activation energy
b) Decreasing the activation energy
c) Increasing the free energy change
d) Decreasing the free energy change
45_Enzymes can be inhibited by:
a) pH
b) Temperature
c) Substrate concentration
d) All of the above
46_The rate of an enzyme-catalyzed reaction is affected by:
a) pH only
b) Temperature only
c) Both pH and temperature
d) Neither pH nor temperature
47_Enzymes are often named by adding "-ase" to the:
a) Product
b) Cofactor
c) Substrate
d) Inhibitor
48_Enzyme kinetics describes:
a) The study of enzyme structure
b) The study of enzyme inhibition
c) The study of enzyme function
d) The study of enzyme classification
49_Competitive inhibitors compete with substrates for:
a) The active site of the enzyme
b) A site other than the active site of the enzyme
c) The product of the reaction
d) The cofactor of the enzyme
50_Enzyme activity is measured in terms of:
a) Vmax
b) Km
c) Kcat
d) All of the above
51_Enzymes increase the rate of reaction by:
a) Decreasing activation energy
b) Increasing activation energy
c) Changing equilibrium constant
d) All of the above
52_Which of the following is NOT a type of enzyme inhibition?
a) Competitive
b) Non-competitive
c) Uncompetitive
d) Substrate-specific
53_Enzyme-substrate specificity is determined by:
a) The shape of the active site
b) The charge of the enzyme
c) The pH of the environment
d) The temperature of the reaction
54_Enzymes are biological catalysts because they:
a) Lower activation energy
b) Increase activation energy
c) Decrease substrate concentration
d) Increase product concentration
55_The rate of an enzyme-catalyzed reaction depends on:
a) The enzyme concentration only
b) The substrate concentration only
c) Both enzyme and substrate concentrations
d) Neither enzyme nor substrate concentrations
56_Allosteric enzymes are regulated by:
a) Competitive inhibitors
b) Non-competitive inhibitors
c) Effector molecules
d) Substrate molecules
57_Enzymes are classified into different classes based on:
a) Their size
b) Their shape
c) Their catalytic activity
d) Their location in the cell
58_Enzyme inhibition can be overcome by:
a) Increasing substrate concentration
b) Decreasing enzyme concentration
c) Changing pH
d) All of the above
59_The turnover number of an enzyme refers to:
a) The number of substrate molecules converted to product per unit time
b) The rate constant of the enzyme-substrate complex formation
c) The maximum velocity of the reaction
d) The concentration of enzyme required for half-maximal activity
60_Enzymes are important in living organisms because they:
a) Regulate gene expression
b) Store genetic information
c) Catalyze metabolic reactions
d) Provide structural support
