Instructions:
Please review the PowerPoint presentation from the face-to-face session, along with any
additional resources you may find. After reviewing the resources, answer the following
questions.
Each question is worth 10 marks.
Duration: 4 weeks
Question 1: Jones and Thatcher developed a spectrophotometric method for analyzing
analgesic tablets containing aspirin, phenacetin, and caffeine. The sample is dissolved in
CHCl3 and extracted with an aqueous solution of NaHCO3 to remove the aspirin. After the
extraction is complete, the chloroform is then transferred to a 250-mL volumetric flask and
diluted to volume with CHCl3. A 2.00-mL portion of this solution is diluted to volume in a
200-mL volumetric flask with CHCl3. The absorbance of the final solution is measured at
wavelengths of 250 nm and 275 nm, at which the absorptivities, in ppm –1 cm–1, for caffeine
and phenacetin are:
caffeine: a250 = 0.0131 and a275 = 0.0485
phenacetin: a250 = 0.0702 and a275 = 0.0159
Aspirin is determined by neutralizing the NaHCO 3 in the aqueous solution and extracting the
aspirin into CHCl3. The combined extracts are diluted to 500 mL in a volumetric flask. A
20.00-mL portion of the solution is placed in a 100-mL volumetric flask and diluted to
volume with CHCl3. The absorbance of this solution is measured at 277 nm, where the
absorptivity of aspirin is 0.00682 ppm–1 cm–1. An analgesic tablet treated by this procedure is
found to have absorbances of 0.466 at 250 nm, 0.164 at 275 nm, and 0.600 at 277 nm when
using a cell with a 1.00-cm pathlength.
Report the milligrams of aspirin, caffeine, and phenacetin in the analgesic tablet.
Question 2: In the DPD colorimetric method for the free chlorine residual, which is reported
as parts per million of Cl2, the oxidizing power of free chlorine converts the colorless amine
N,N-diethyl-p-phenylenediamine to a colored dye that absorbs strongly over the wavelength
range of 440–580 nm. Analysis of a set of calibration standards gave the following results
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A sample from a public water supply is analyzed to determine the free chlorine residual,
giving an absorbance of 0.113. What is the free chlorine residual for the sample in parts per
million Cl2?
Question 3: Bonert and Pohl reported results for the atomic absorption analysis of several
metals in caustic suspensions produced during the manufacture of soda by the ammonia-soda
process.
(a)The concentration of Cu was determined by acidifying a 200-mL sample of the caustic
solution with 20 mL of concentrated HNO 3, adding 1 mL of 27% w/v H 2O2and boiling for 30
min. The resulting solution was diluted to 500 mL, filtered, and analyzed by flame atomic
absorption using matrix-matched standards. The results for a typical analysis are shown in the
following table.
Determine the concentration of Cu in the caustic suspension.
(b)The determination of Cr was accomplished by acidifying a 200-mL sample of the caustic
solution with 20 mL of concentrated HNO 3, adding 0.2 g of Na2SO3and boiling for 30 min.
The Cr was isolated from the sample by adding 20 mL of NH 3, producing a precipitate that
includes the chromium as well as other oxides. The precipitate was isolated by filtration,
washed, and transferred with wash water to a beaker. After acidifying with 10 mL of HNO 3,
the solution was evaporated to dryness. The residue was redissolved in a combination of
HNO3and HCl and evaporated to dryness. Finally, the residue was dissolved in 5 mL of HCl,
filtered, diluted to volume in a 50-mL volumetric flask, and analyzed by atomic absorption
using the method of standard additions. The atomic absorption results are summarized in the
following table.
Report the concentration of Cr in the caustic suspension.
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Question 4: Label the below schematic diagram represent an atomic Absorption
spectrometer
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