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Salvia Officinalis: Investigating The Anti-Diabetic Potential of Using A Yeast-Based Bioassay and Molecular Analysis

The document is a project dissertation by Duggaraju Srilekha submitted for a Master of Science in Microbiology, focusing on the anti-diabetic potential of Salvia officinalis through yeast-based bioassays and molecular analysis. It includes sections such as an abstract, introduction, aim and objectives, literature review, materials and methods, results and discussion, conclusion, and references. The work is certified as original and has not been submitted elsewhere for any degree.

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chanikyachandrasekhar
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33 views6 pages

Salvia Officinalis: Investigating The Anti-Diabetic Potential of Using A Yeast-Based Bioassay and Molecular Analysis

The document is a project dissertation by Duggaraju Srilekha submitted for a Master of Science in Microbiology, focusing on the anti-diabetic potential of Salvia officinalis through yeast-based bioassays and molecular analysis. It includes sections such as an abstract, introduction, aim and objectives, literature review, materials and methods, results and discussion, conclusion, and references. The work is certified as original and has not been submitted elsewhere for any degree.

Uploaded by

chanikyachandrasekhar
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
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INVESTIGATING THE ANTI-DIABETIC POTENTIAL OF Salvia

officinalis USING A YEAST-BASED BIOASSAY AND MOLECULAR


ANALYSIS
A project dissertation submitted by in partial fulfilment for the requirement of the degree of

MASTER OF SCIENCE

IN

MICROBIOLOGY

BY

DUGGARAJU SRILEKHA

(P23MI0008)

DEPARTMENT OF MICROBIOLOGY AND BIOTECHNOLOGY FACULTY OF


ARTS AND SCIENCE BHARATH INSTITUTE OF HIGHER EDUCATION AND
RESEARCH

(Declared as deemed university under section 3 of UCG Act 1956, vide

notification No.F.9-5\2000-U.3)

CHENNAI- 73
APRIL 2025
.

BHARATH INSTITUTE OF HIGHER EDUCATION AND RESEARCH

(Declared as deemed university under section 3 of UCG Act 1956, vide notification

No.F.9-5\2000-U.3)

DEPARTMENT OF MICROBIOLOGY AND BIOTECHNOLOGY

This is to certify that “INVESTIGATING THE ANTI-DIABETIC


POTENTIAL OF Salvia officinalis USING A YEAST-BASED BIOASSAY
AND MOLECULAR ANALYSIS” is a Bonafide work done D.Srilekha (P23MI0008)
for the major project in Microbiology during the academic year 2024-2025

PROJECT GUIDE HOD

(M.rs R. CATHERINE LAURENCIA) ( Dr. VASUKIDEVIRAMACHANDRAN)

Submitted for the Viva Voice held on ...................................................in the Department of

Microbiology and Biotechnology, Faculty of Arts and Science, BIHER.

INTERNAL EXAMINER EXTERNAL EXAMINER


DECLARATION

I hereby declare that the project entitled that “INVESTIGATING THE ANTI-DIABETIC
POTENTIAL OF Salvia officinalis USING A YEAST-BASED BIOASSAY AND
MOLECULAR ANALYSIS’’ ” submitted to Bharath Institute of Higher Education and
Research, Chennai in partial fulfilment of the requirement for the award of the degree of Master
of Science in Microbiology is the record of the original work carried out by me under the
guidance of M.rs R. CATHERINE LAURENCIA further declared that the result of the work
have no I t submitted to any other university or institute for the award of any degree or diploma.

PLACE: Chennai SIGNATURE OF THE STUDENT

DATE: Duggaraju Srilekha (P23MI0008)


ACKNOWLEDGEMENT

I would like to Express my Sincere gratitude to our Management for their valuable Support and
encouragement in technological Upgrade and novel project and providing us much more
facilities. I take great pleasure in expressing our sincere thanks to our Additional registrar Dr.R.
HARIPRAKASH for their support and encouragement. I extend my thanks to Dean of arts
Dr. A. MUTHUKUMARMARAVEL and Dean of Science Dr. V. KALAIRASI, for
providing sufficient facilities for the completion of this project. I would like to express my
sincere thanks to Head of the Department Dr. VASUKIDEVI RAMACHANDRAN and guide
M.rs R. CATHERINE LAURENCIA for her kind support, guidance, and encouragement
throughout the study.

DUGGARAJU SRILEKHA
CONTENTS

SL.NO TITLE PAGE N.O


1
ABSTRACT 1

2
INTRODUCTION 2

3
AIM & OBJECTIVES 16

4
REVIEW OF LITERATURE 17

5
MATERIALS & METHODS 22
6
RESULT AND DISCUSSION 35
7
CONCLUSION 44

8
REFERENCES 45
ABBREVIATIONS

 S. officinalis – Salvia officinalis


 S. cerevisiae – Saccharomyces cerevisiae
 DPPH – 2,2-Diphenyl-1-picrylhydrazyl
 FRAP – Ferric Reducing Antioxidant Power
 TAC – Total Antioxidant Capacity
 LPO – Lipid Peroxidation
 MPO – Myeloperoxidase
 MDA – Malondialdehyde
 SOD – Superoxide Dismutase
 CAT – Catalase
 GPx – Glutathione Peroxidase
 HXK – Hexokinase
 G6PD / ZWF1 – Glucose-6-Phosphate Dehydrogenase
 RNA – Ribonucleic Acid
 DNA – Deoxyribonucleic Acid
 PCR – Polymerase Chain Reaction
 RT-PCR – Reverse Transcription PCR
 cDNA – Complementary DNA
 OD – Optical Density
 ATP – Adenosine Triphosphate

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