User Manual

Please read the manual before installation and operation.

Drawell International Technology Limited

Chongqing Drawell Instrument CO,.Ltd

Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China
Tel: 0086-023-63268643

Shanghai Drawell Scientific Instrument CO.,Ltd.

Add: Suite 1117,Lane561 XiuChuan Rd.,PuDong New Area,Shanghai,China

Web : www.drawell.com.cn Email : sales05@drawell.com.cn

 User ManualV2.0

Contents
I. Overview.....................................................................................................................................- 1 -
1. Application................................................................................................................................. - 1 -
2. Principles................................................................................................................................... - 1 -
II.Main performance......................................................................................................................- 2 -
1. Technical indicators................................................................................................................. - 2 -
2. Performance characteristics................................................................................................... - 2 -
3. Working condition of instrument.............................................................................................- 3 -
III. Names of instruments parts...................................................................................................- 4 -
Composition of instrument structure.......................................................................................... - 4 -
IV. Installation methods of instrument....................................................................................... - 5 -
1. Inspection before installation.................................................................................................. - 5 -
2 Installation conditions................................................................................................................- 5 -
3. Instrument Installation..............................................................................................................- 5 -
V. Operation Instruction............................................................................................................... - 6 -
1. Reagent, instrument and appliances.....................................................................................- 6 -
2. Pre-treatment of sample:.........................................................................................................- 7 -
2.1 Pre-treatment of food samples.............................................................................................- 7 -
2.2 Pre-treatment of feed samples.............................................................................................- 8 -
3. Instrument operation................................................................................................................ - 8 -
4、Experimental operation........................................................................................................- 13 -
VI. Common faults and treating methods............................................................................... - 14 -
VII.Daily maintenance of instruments......................................................................................- 15 -
VIII.Matters need attention........................................................................................................ - 15 -
IX. Supplementary...................................................................................................................... - 17 -
X. Temperature Value Reference............................................................................................ - 17 -
 User ManualV2.0

Caution: The person in charge needs to know if instrument is not used

according to the manners required by manufacturer; the protection provided by
instrument itself may be weakened.

Caution: Carefully process various solvents used in analysis according

to the safety regulations of laboratory. Refer to the corresponding Material
Safety Data Sheet. Wear lab coat, eye-protection glasses and rubber gloves, no
matter what time. Be careful while processing hot reagents.

Caution: Risk of electric shock! Only professional and qualified

personnel can open the machine cover and the panel.
 User ManualV2.0

I. Overview

1. Application

SOX406 Fat Analyzer is the crude fat analyzer designed according to GB/T
14772-2008, can measure the fat in food, dairy products and animal feed,
meanwhile, it also applies to measure the soluble organic compounds in
detergents, pharmaceuticals, petrochemicals, fiber products, soil and sludge.

For example:

1. Fast and safely measure the fat in food, feed, grains and seeds;

2. Extract oil and grease in waste water and sludge;

3. Extract the semi-volatile organic compounds in soil, such as insecticides

and herbicides;

4. Extract the plasticizer in plastics, the rosin in paper and paperboard, the
grease in leather and so on;

5. Decomposition pretreatment of solid sample for the gas phase and liquid
phase chromatography.

2. Principles

The instrument uses the gravimetric method to measure the fat content
according to Soxhlet extraction principle. That is, after the sample is
extracted by using anhydrous ether or petroleum ether, the fat is extracted
from the sample, and then dried and weighed; the calculated mass
differences of the extraction cup before and after extraction is the fat content.

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II. Main Performance

1. Technical Indicators

1. Temperature control range: room temperature +5-300 ℃

2. Temperature control accuracy: ±1 ℃

3. Processing capacity: 6 pcs/ batch

4. Volume of solvent cup: 150 mL

5. Sample amount: 0.5-15 g (constant quantity: 2-5 g)

6. Solvent recovery rate: ≥85%

7. Measurement range: 0-100%

8. Rated power: 1KW

9. Operating voltage: 220 VAC ±10% 50Hz

10. External dimensions (mm): 650×320×700

11. Weight: 35 kg

2. Performance Characteristics

1. True color LCD screen, micro-computer control system;

2. The over-temperature alarm and the timed reminder;

3. Triple alarm system with sound and text prompting on LCD screen;

4. Equipped with both the timing function and the chronograph function;

5. Overall metal heating block is adopted for heating, with wide temperature
control range and high temperature control accuracy;

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6. Lift connection of linear bearing conduction technology, flexible and

comfortable for lifting operation;

7. Rich content of the interface, synchronously display the setting

temperature, the actual temperature, the setting time and the heating time;

8. The electric circuit and the extraction space are totally separated, which
enhances the security;

9. Unique air layer insulation technology keeps the shell of machine body
under normal temperature and this instrument is equipped with the dual
functions of heat insulation and heat preservation;

10. Compared with traditional Soxhlet analysis, the extracting speed is faster
and the efficiency is higher.

3. Working Condition of Instrument

1. Input voltage: 220 V 50 Hz; air switch and electric leakage protection
switch need to be equipped;

2. The instrument shall be installed near water source and drainage pond,
and equipped with suitable electric outlet;

3. The power configuration shall conform to the requirements of the power

supply, preventing electric overload; it must be equipped with a ground wire,
separate power supply switch and safety device, ensuring the safety of
operators;

4. The instrument shall be installed far away from high-power electrical

appliances to avoid the interference of strong electromagnetic field;

5. The instrument shall be installed at the opening with good ventilation.

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III. Names of Instruments Parts

Composition of instrument structure

1. Sliding bead 2. Condensation tube 3. Plug cock 4. Extraction cup

5. Wrench 6. Power interface 7. Power switch

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IV. Installation Methods of Instrument

1. Inspection before Installation

After unpacking of instrument, check and verify all stated main machine and
accessories according to the packing list attached with the equipment, and
check if there is any damage, if yes, please contact with the manufacturer
timely (Please keep the damaged parts).

2 Installation Conditions

(1) The instrument shall not be installed at the places where expose to direct
sunlight, or is too cold, too hot or humid; the general room temperature shall
be kept between 10 ℃-28 ℃;

(2) The instrument shall be installed at the working position near water
source and drainage pond where is also equipped with power outlet, no
matter water supply valve or power supply, their positions from the instrument
shall not be more than 1m, to assure the convenience of using and operating;
the water flow is required at 1~2 L/min, the water supply shall comply with the
required water pressure, and water temperature cannot be more than 20 ℃;

(3) The drainage pond shall be more than 10cm below the drainage exit of
instrument to make sure the natural drainage unobstructed

(4) The power configuration shall meet the requirements for power supply,
the ground wire must be equipped, and there is separate power supply
switch and safety device to ensure the electricity safety of operators;

(5) The instrument shall be installed at the place far away from high-power
electrical equipment; there is no vibration, no corrosive liquid and no
interference of strong electromagnetic fields at the working place.

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3. Instrument Installation

The instrument shall be stably placed on the experimental bench, the back of
the instrument shall be 20cm away from the wall, the distance between
power outlet and the machine shall not be more than 1m, air switch, electric
leakage protection switch and reliable ground wire shall be also equipped.
For the water inlet and outlet at the left side of instrument, see Figure 1:

Figure 1

1. Inlet of condensate water, connected with the valve of running water.

2. Outlet of condensate water, connected with the drainage pond.

V. Operation Instruction

1. Reagent, Instrument and Appliances

1. Anhydrous ether (analytically pure), petroleum ether (analytically pure,

boiling range of 30-60 ℃)

2. Analytical balance（0.0001 g）

3. Filter paper with diameter of 90 mm

4. 100 mL measuring cylinder

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5. Drying cabinet（drying oven）

6. Laboratory pulverizer

7. Mortar and pestle

8. Dryer

2. Pre-treatment of Sample:

2.1 Pre-treatment of Food Samples

2.1.1 Solid sample: take a representative sample of 200g at least, use mortar
to mash, porphyrize and mix evenly, and place in the airtight glass vessel; for
the samples not easy to mashed and porphyrized, it shall be cut to be fine
particles and passed through the round-hole mesh of diameter 1.0 mm, and
then be placed in the airtight glass vessel;

2.1.2 Powder sample: take a representative sample of 200g at least (if the
powder particle is relatively big, it shall also be placed in mortar for
porphyrization), mixed evenly, and passed through the round-hole mesh of
diameter 1.0 mm, and then be placed in the airtight glass vessel;

2.1.3 Pasty sample: take a representative sample of 200g at least, mix

evenly and place in the airtight glass vessel;

2.1.4 Solid and liquid sample: according to the proportion of solid and liquid,
take a representative sample of 200g at least, use organization masher to
mash;

2.1.5 Meat products: remove the non-edible part, take a representative

sample of 200g at least, use meat grinder to grind twice at least, mix evenly
and place in the airtight glass vessel;

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2.2 Pre-treatment of Feed Samples

2.2.1 Samples have been smashed or the fat content is relatively low, to
process by referring to 2.1;

2.2.2 If the sample is not easy to be smashed or the fat content is high (more
than 200 g/kg), it must be pre-extracted in advance.

3. Instrument Operation

1. Operation control panel of instrument, see Figure 2

Figure 2

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Figure 3

2. The setting of temperature and time: after starting up, press “Enter”

to enter into “heating” menu, see Figure 4.

Figure 4

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After setting temperature and time well, press “ ” to start heating, the
display interface of the heating process is shown as the following, see
Figure 5.

Figure 5
After the setting time is up, the heating will stop.

If the temperature exceeds the setting temperature of instrument by 25℃, the

instrument will remind over-temperature, such as Figure 6

Figure 6

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4. Setting of system configuration: after the initialization of starting up is

finished, press arrow key to move cursor to “Set”, press key to

enter into “Set” interface, see Figure 7.

Figure 7

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Temperature of sensor: temperature collected by temperature sensor

Calibrated temperature: actual temperature of heating device; after inputting,

it can correct the display temperature of system.

Mode of timing: for counting, the heating time starts counting; for counting
down, the heating time starts counting down.

Timing point: starting heating, the instrument starts timing from the time when
heating is started. If the temperature is reached, the instrument starts timing
from the time when the setting temperature is reached.

Besides, during the heating process, press “ ” and the heating can be

stopped temporarily, after press again, the heating restars; press “ ”, the
heating can be stopped for emergency, and it goes back to the function
selection interface, see figure 3.

5. In normal operation, press return key, it can go back to the instrument

system operation interface, see figure 3.

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4. Experimental Operation

1. Sample binding up, use analytical balance to weigh 2-5g of processed

sample (accurate to 0.0001g), place in the filtration paper cylinder (the
filtration paper cylinder shall be rolled well by using the roll bar included with
instrument), and then put in the filter paper holder, use magnet to hold the
filter paper holder;

2.Dry the extraction cup of constant weight, record the qualitative properties
of the extraction cup, and then put 60 mL of petroleum ether or anhydrous
ether into the extraction cup, and then place the extraction cup on the
heating plate;

3.Press down the wrench, make the seal washer press the extraction cup
tightly, move the sliding bead, put the filtration paper cylinder in extraction
cup to make the sample completely immersed into solvent;

4. Turn on the power supply of instrument, open condensate water, set the
temperature and the required time;

5. After extracting for a certain period of time, move the sliding bead, raise
the filtration paper cylinder to high-order position, and carry out extraction;
after the extraction is finished, turn off the plug cock on condensation tube,
make solvent completely evaporate in the liquid storage tank at the lower
part of condensation tube, and then turn on the plug cock to make solvent
flow down, repeatedly wash out for several times to make sure that no
residual fat in the sample, at last turn off the plug cock to make the solvent in
extraction cup completely evaporate in the liquid storage tank, and then stop
heating;

6. When the heating plate and the extraction cup is cooled to room
temperature, lift up the wrench, open the plug cock and recycle the solvent in
liquid storage tank;

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7.Take the extraction cup from the heating plate and put in the drying cabinet
for drying water and residual organic solvent, and then move into the dryer
and weigh after cooling, calculate the fat content;

8. Turn off power, close condensate water.

VI. Common Faults and Treating Methods

No Causes of Failure Analysis Reasons Treating Methods

.
1 The whole The fuse is burnt Replace the fuse
machine without The power cord is not plugged Plug the power cord
electricity firmly well
2 Solvent gas leaks 1. The flow of condensate water Increase water flow
during the is not enough Replace the rubber ring
process of 2. The rubber ring at the lower Replace the
experiment part of condensation tube is condensation tube
aging, sealing failure
3. The condensation tube is
damaged.
3 Instrument does Check whether there is Turn off instrument,
not work interference from strong remove the source of
normally, system magnetic field or strong electric interference and restart
halted. field around the instrument machine.

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VII. Daily Maintenance of Instruments

1. The instrument needs heating when it works, so good ventilation and

cooling conditions are needed;

2. Try to use the same kind of solvent for the same set of extraction system
within a short time, to prevent the cross contamination of solvent;

3. Use damp cloth to wipe the instrument clean, it is important that the
cleaning cloth must be wet to avoid generating static electricity; a piece of
abrasive cloth shall be used for the cleaning of heating plate to clean all
ash to improve heat transferring;

4. Pay attention to the cleaning of extraction cup at ordinary times, it needs

cleaning regularly;

5. Make sure if there is seaweed plant growing in the cooling unit; if

necessary, please use 1 mol/L hydrochloric acid or hypochlorite solvent to
clean up.

VIII. Matters Need Attention

1. Please read the operation instructions carefully and operate according to

its instructions, the personnel who are not familiar with the operation
instructions shall not use instruments;
2. Damp cloth must be used to clean the surface of the leaching and
extraction system, especially for glass products, and dry cloth will bring the
electrostatic danger;

3. The maintenance for the leaching and extraction system shall be carried
out strictly in accordance with operating procedures, the replacement of
glass products, accessories and wires shall be carried out under the

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guidance of post sales engineers of Hanon, but all the other necessary
maintenance work must be handled by the post sales engineers of Hanon;

4. Hanon Company shall not guarantee for any unauthorized modification,

transformation and recondition on instrument, and shall assume no
responsibly for any personal casualty or property loss caused thereby;

5. Main power must be cut off while disassembling the cover and replacing
the protective tube;

6. There are glass accessories in instruments; therefore, it needs to be

careful during the process of carrying and transporting.

7. If the instrument will not be used for a long time, the solvent in
condensation tube shall be discharged;

8. Try to use the same kind of solvent for the same set of extraction system
within a short time, to prevent the cross contamination of solvent;

9. There is explosion danger while taking out or putting back organic solvent,
the operator shall avoid solvent being exposed in the environment with
naked flame and static electricity;

10. Do not touch the heating block at the heating state, in order to avoid
scald;

11. During the experiment process, there shall be specially-assigned person

to guard the laboratory.

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IX. Supplementary

Since the date of the product being sold (subject to the date of the issued
invoice), the complete machine has one year warranty, but the following
situations are beyond the scope of warranty:

1. Over the warranty period;

2. Damage caused due to improper use;

3. Damage caused by self-disassembling without permission of

manufacturer;

4. Damage caused by improper transportation or storage.

X. Temperature Value Reference

As affected by room temperature, the heat transfer efficiency from heating

plate to extraction cup and other factors, the heating temperature needs to be
regulated properly; the suitable temperature shall be controlled to make the
dropping speed of solvent at about 3-5 drops per second. For the extraction
temperature of common organic solvent, please see the form below.

Solvent Extraction temperature (℃)

30-60 Petroleum ether 80
Ethyl ether 60
Acetone 76
Dichloromethane 60
Hexane 89
Methanol 85
Ethanol 98
Chloroform 82
Benzene 100

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※Note: if the organic reagents that not mentioned in the form are used,
the extraction temperature could be increased by 20℃ on the basis of
boiling point.

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Drawell International Technology Limited

Chongqing Drawell Instrument CO,.Ltd

Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District, Chongqing,China
Tel: 0086-023-63268643

Web : www.drawell.com.cn
Email : sales05@drawell.com.cn