BPMT Blood transfusion

I – YEAR

Examination Pattern

Theory Practicals Subject

Total
Sr Paper Subject IA Final Theory IA Final Practical
No Total Total
-- -- Max: 90 -- -- Max: 110 Max: 200
Min: 45 Min: 55 Min: 100
1 Paper I Basic Sciences 30 60 90 30 80 110 200

2 Paper Introduction to 30 60 90 30 80 110 200

II transfusion
technology, Lab
management
3 Paper Introduction to 30 60 90 30 80 110 200
III blood bank,
Legal aspects
 Syllabus for Paper I – Basic Sciences

Sr. Topic Lecture /

No Demo
Hours
1. Introduction to Anatomy 2
2. Introduction to various body parts and systems 2
3. Bones (Only nomenclature) 4
4. Joints (Only nomenclature) 2
5. Anatomy & Functions of respiratory System (Only Organ nomenclature and 4
brief functions)
6. Anatomy & Functions of cardiovascular system (Only Organ nomenclature, 4
important arteries and veins / sites for venepuncture and brief functions)
7. Anatomy & Functions of Abdominal Organs , GI system (Only 4
nomenclature and brief functions)
8. Anatomy & Functions of Pelvic Organs (Only nomenclature and brief 4
functions)
9. Anatomy & Functions of Nervous System (Only nomenclature and brief 4
functions)
10. Anatomy & Functions of Endocrine glands (only nomenclature, site and 4
brief functions )
11. Genetics (Only introduction and brief functions) 2
12. Organization of Human body, cell 2
13. Composition and functions of blood 4
14. Introduction to transfusion technology 2
15. Role and responsibilities of transfusion technician 2
16. General precautions in laboratory 2
17. Laboratory safety measures 2
18. Biological specimens and their types 2
19. Methods of collection of biological specimens 4
20. Preservation and disposal of biological specimens 2
21. Modes of transmission of diseases 2
22. Control and prevention of infections 2

As all the contents are of basic nature and at undergraduate level, all these lecture are to be
conducted by the parent department.
Paper – II Introduction to transfusion technology, Lab management

1. Introduction to transfusion: Role and responsibility of transfusion technician, 2

safety measures and first aid.
2. Cleaning and care of general laboratory glassware and equipment. Principles,
functions and uses of balances, centrifuge machines, colorimeters. 2
3. Collection and recording of biological specimens, separation of serum plasma 2
preservation and disposal of biological samples/materials
4. Standard solution: Various std. solutions used, their preparation; storage of 1
chemicals
5. Laboratory Management and Planning. The reception and recording of 4
specimen, cataloguing and indexing maintenance of laboratory records.
6. Introduction to microbiology lab, various types of specimens received 2
7. A knowledge of working and maintenance of the following Incubators, 3
Refrigerators, Water baths, Ovens, Steamers, Autoclaves, Inspissator,
Centrifuges, Vaccum Pumps, Water Steel. Cleaning and sterilization of
syringes and needles. Sample glass wares.
8. Sterilization: Methods of sterilization and their uses. Chemical, dry heat, 2
steam sterilization, Tyndallization, filtration, sterilization by ultraviolet light.
9. Care and use of microscope. Dark ground illumination, fluorescence and 2
microscopy.
10. Introduction and scope of Pathology 2
11. Introduction to Pathology lab, various types of specimens received 2
12. Laboratory Management and Planning. The reception and recording of 2
specimen, cataloguing and indexing maintenance of laboratory records .
13. Working and maintenance of instruments 4
14. General principles of Haematology techniques collection, fixation, 3
processing & routine staining, Haemoglobin, TLC, DLC, Peripheral smear,
automatic cell counter
15. General principles of Blood Bank techniques antigen, antibody, ABO & Rh 3
system
16. Systemic Pathology, basis and morphology of common disorders like anemia, 3
leukemia, AIDS, TB, Hepatitis & malaria.
17. Maintenance and medico legal importance of records and specimens. 3
Paper III: Introduction to blood bank, Legal aspects

1. Introduction to blood bank

2. Instruments, equipments used in blood bank
3. General care of instruments
4. Record keeping in blood bank
5. Work flow in blood bank
6. General precautions
7. Role of NGO
8. Legal aspects related to blood bank
9. General precautions in handling of blood
10. Disposal methods
11. Donor motivation
12. Preservation & storage of blood for transfusion and storage effects
 BPMT Blood Transfusion
2nd YEAR
Rotational postings (II Year)
Biochemistry - 1 month
Microbiology 2 month
Examination pattern

Theory Practicals Subject

total
Sr Paper Subject IA Final Theory Ia Final Practical
no total total
-- -- Max: 90 -- -- Max: 110 Max: 200
Min: 45 Min: 55 Min: 100
1 Paper I Clinical 30 60 90 30 80 110 200
pathology,
biochemistry
and general
lab methods
2 Paper Elementary 30 60 90 30 80 110 200
II microbiology &
serology
3 Paper Elementary 30 60 90 30 80 110 200
III haematology

Paper I : Clinical Pathology, Biochemistry and General lab methods

CLINICAL PATHOLOGY
1 Routine examination of urine for sugar 4 4
2 Examination of urine for Protein, Sp.gravity 4 4
3 Urobilinogen 4 4
4 Examination of urine for bile salt & bile pigment 2 2
5 Microscopic examination of urine 2 2
6 Examination of faeces including test for occult blood 1 1

ELEMENTARY BIOCHEMISTRY
1 Elements, atoms and molecules 1 1
2 Atomic weight, molecular weight and equivalent wt 1 1
3 Preparation of normal solution & molecular solution 1 1
4 Preparation of stock & buffer solution 4 4
5 Acids & Alkalies 4 4
6 Ph & its determination 4 4
7 Chemical balance & measuring cylinders 2 2
8 Photoelectric Colorimeter 2 2
9 Introduction of flame photometer & polarimeter 2 2
10 Quality control tests – Chemical analysis of isotonic Saline, 6 6
5% Glucose, Ringer lactate, IGS, CPD soln and Other
crystalloids fluids
 General Laboratory Methods / Eqpts Theory Practical
38 Refrigerator 2 2
39 Centrifuges 2 2
40 Waterbath 2 2
41 Electrophoresis apparatus 4 10
42 Biosafety measures 5 5

Paper II: ELEMENTARY MICROBIOLOGY & SEROLOGY

ELEMENTARY MICROBIOLOGY & SEROLOGY
1 Classification of Bacteria, sources of infection, Prevention of 6 6
contamination
2 Differentiation of bacteria, fungi and viruses 4 4
3 Structure of bacteria- capsules, flagella, spores & their 4 4
importance
4 Gram’s staining 4 4
5 Acid – Fast bacilli & Ziehl Neelson staining 6 6
6 Fungi- general & staining 6 6
7 Staining for blood borne parasites 6 6
8 Hepatitis B & C viruses 6 6
9 HIV Viruses I & II 6 6
10 Antigen & Antibody 6 6
11 Antigen Antibody reactions and factors affecting them 6 6
12 HIV antibody test (HIV- I & II) 4 4
13 Test for HBsAg, HCV, CMV & Toxoplasma 4 4
14 VDRL/ RPR Test 4 4

Paper III: ELEMENTARY HAEMATOLOGY

ELEMENTARY HAEMATOLOGY
1 Collection of capillary & venous blood and anticoagulants 6 6
2 Estimation of haemoglobin by copper sulphate (CuSO4), 6 6
Sahlis and CyanmethHb method
3 Estimation of packed cell volume and ESR 6 6
4 Estimation of total leucocyte count 6 6
5 Preparation and staining of peripheral blood smear 6 6
6 Examination of peripheral smear for parasites 6 6
7 Estimation of differential leucocyte count 6 6
8 Estimation of platelet count 2 2
9 Estimation of glucose six phosphate dehydrogenase (G6P 2 2
10 Detection of foetal cells in maternal circulation 6 6
11 Haemoglobinopathies
a Abnormal haemoglobins, thalassaemia and sickle cell 6 6
anaemia
b Alkali denaturation test 2 2
c Test for sickling 4 4
d Haemoglobin electrophoresis 10 10
e Estimation of plasma haemoglobin 2 2
12 Mechanism of coagulation of blood 4 4
13 Collection & handling of blood samples for Coagulation 4 4
studies
 14 Bleeding & clotting time 2 2
15 Prothrombin time 6 6
16 Activated partial thromboplastin time 6 6
17 FDP importance & tests in serum and urine 4 4
18 Principle of factor VIII Assay 4 6

3rd Year

Examination pattern

Theory Practicals Subject

total
Sr Paper Subject IA Final Theory Ia Final Practical
no total total
-- -- Max: 90 -- -- Max: 110 Max: 200
Min: 45 Min: 55 Min: 100
1 Paper I Clinical 30 60 90 30 80 110 200
haematology and
ward work
2 Paper Screening, 30 60 90 30 80 110 200
II grouping before
blood transfusion
3 Paper Transfusion 30 60 90 30 80 110 200
III reactions, Recent
advances in
transfusion

(Students will be posted in wards for 2 months)

Paper I: CLINICAL HAEMATOLOGY and WARD WORK

CLINICAL HAEMATOLOGY and WARD WORK

1 Circulatory System 4 4
2 Pulse, heart rate, blood pressure, temperature, 6 6
respiratory rate
3 Respiratory System 6 6
4 Venepuncture 14 14
5 Catheterisation 5 5
6 Insertion of CVP line and Femoral catheter 10 10
7 Thrombosis 2 2
8 Clotting and Coagulation mechanism 5 5
9 Cell injury and ischaemia 5 5
10 Shock and resuscitation 10 5
11 Anaphylaxis 4 4
 12 Vasovagal reaction 4 4
13 Intubation and Resuscitation in the ward 4 4
14 Handling of nursing station and maintenance of registers 4 4
15 I V Fluids 4 4
16 I V infusion 4 4
17 Anemias 4 4
18 Thrombocytopenia 4 4
19 Leukemias 4 4
20 Lymphomas 4 4
21 Neoplasia 4 4
22 Basic pharmacology in Onco Haematology 4 4
23 Role of Chemotherapy and radiotherapy in Onco 4 4
Haematology
Paper II: Screening, grouping before blood transfusion

Screening, grouping before blood transfusion Theory Practical

1 Blood groups in general and their importance 6 6
2 ABO Blood group system & subgroups 3 3
3 ABO Blood grouping by various techniques 3 3
4 Rh Blood group system 3 3
5 Rh Blood grouping phenotype & genotype 3 3
6 Detection of Du antigen 2 2
7 Compatibility test 2 6
8 Preparation of serum, cells suspension (various percentages) and 4 4
washing of RBC’s
9 Antiglobulin Test (DAT & IAT) 4 8
10 Detection of Immune antibodies including Rh antibodies, elution 4 8
of antibodies
11 Titration of Anti A, Anti B & Anti D anti sera 4 8
12 Detection & Titration of Immune antibodies Anti A, Anti B 4 8
13 Compatibility test in AIHA 2 8
14 Compatibility test in major cardiopulmonary surgery 2 2
15 Group specific substances including lectins and their preparation 4 4
16 Blood donor motivation propaganda 4 4
17 Selection & bleeding of donors 4 4
18 Adverse reactions in blood donors & their management 4 4
19 Preservation & storage of blood for transfusion and storage effects 4 4
20 Hazards of blood transfusion 4 4
21 Investigation of transfusion reactions 4 4
22 Blood component therapy 12 12
23 Preparation of various blood components & their Quality control 10 10
24 Composition and fractions of plasma 10 10
25 Indications of blood & blood component transfusion 5 2
26 Shock & resuscitation 6 3
27 Transfusion transmitted diseases 4 2
28 Autologous transfusion 4 2
29 Organization of blood bank services & FDA Licensing of Blood 4 2
Bank
30 Disposal of infected blood units and other infected material 2 2
31 Crystalloid and colloid solutions 3 3
32 Principles and various methods of sterilization 3 3
33 Glassware & rubber items used in Transfusion medicine-cleaning 3 3
and maintenance
34 Disposable blood and fluid administration sets – components and 3 3
Quality control
35 Elisa Reader- functions, operation and maintenance 4 4
36 Introduction to cell separator- functions, operation and 10 10
maintenanceto Stem cells, sources, collection and Cryopreservation
37 Introduction 10 10
Paper III: Transfusion reactions, Recent advances in transfusion

1. Transfusion reactions and their identification

2. Preparation and maintenance of emergency tray

3. Maintenance and checking of instruments, equipments used during transfusion

reactions

4. Role of technician during transfusion and transfusion reactions

5. Recent advances in transfusion technology