SUBJECT: PHARMACEUTICS I

PRACTICAL MANUAL BOOK

LAB MANUAL
PHARMACEUTICS I
(B. Pharm 1ST SEM)

I Q City Institute of Pharmaceutical Sciences

Durgapur | District: Burdwan, West Bengal | Pin:713205

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Contents
Exp. No. Name of the experiment Page no.
1 Preparation of simple syrup 3-4

2 Preparation of aqueous iodine solution 5-6

3 Preparation of Calamine lotion 7-8

4 Preparation of Sulphur ointment 9-10

5 Preparation of Magnesium Hydroxide mixture 11-12

6 Preparation of Turpentine Liniment 13-14

7 Preparation of Effervescent granules 15-16

8 Preparation of Dusting powder 17-18

9 Preparation of Piperazine citrate elixir 19-20

10 Preparation of Iodine Throat Paint 21-23

11 Preparation of ORS powder 24-26

12 Preparation of Iodine gargle 27-29

13 Preparation of Chlorhexidine mouthwash 30-32

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 1
PREPARATION OF SIMPLE SYRUP
Aim: To prepare and submit 30 ml of Simple Syrup IP.

Reference:
Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 155 – 156.
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, stirrer etc.
Chemical Required:
Sucrose and purified water.
Principle:
Syrups are sweetened, viscous, concentrated solutions of sucrose or other sugars in water or anyother
suitable aqueous vehicles. These are further classified into 2 classes.
1. Simple flavored syrups
2. Medicated syrups
Simple flavor syrups:
Do not contain any medicament or drug. These syrups are used as a vehicle for other Liquid
preparation to mask the disagreeable taste of drug.
Medicated syrups:
These contain some medicinal substance along with their other additives. Sucrose concentration in
simple syrup is a 66.7 %w/w.
Formula:

Sl. No. Ingredients Official formula Required quantities

1 Sucrose 667gm
2 Purified water, quantity 1000ml 30ml
sufficient to produce (q. s)

Calculations:

Procedure:
Add water to sucrose in a beaker and heat on water bath until sucrose dissolves add sufficient boiling
water to produce the final volume. Filter hot syrup through cotton wool. cool the syrup and
preservatives may be added for stability. Add more purified water to make up the required volume.
Filter and transfer in a suitable container.

Category:
Sweetening agent, vehicle
Use:
Simple Syrups are used as vehicles for drugs such as antibiotics, Antihistaminic, antitussives and
vitamins.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Storage:
Stored in a well closed container at a temperature not exceeding 250C.

Label:
SIMPLE SYRUP IP
30ml
Composition:
Sucrose BATCH NO. MFG. DATE:
Purified water
MFG. LIC. NO.: EXPIRY DATE:
Category: Sweetening agent, vehicle

FOR INTERNAL USE ONLY.

Use: Simple Syrups are used as vehicles for drugs such as antibiotics, Antihistaminic, antitussives
and vitamins.
Storage: Stored in a well closed container dark in a cool place and temperature not exceeding 250C
MFG. BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 2
PREPARATION OF AQUEOUS IODINE SOLUTION

Aim: To prepare and submit 30ml of aqueous iodine solution.

Reference:
Gupta A.K., Jain V.K., Pharmaceutics-I Practical Note Book, CBS Publishers & Distributors
Pvt.Ltd.2020; 15
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, funnel etc.
Chemical Required:
Iodine, potassium iodide and purified water
Principle:
In pharmaceutical practice solution are defined as liquid preparation containing one or more chemical
substances usually dissolved in water. Aqueous iodine solution is also known as Lugol’s Solution
and contain 5%w/v of iodine and 10% w/v of potassium iodide.

Formula:

Sl. No. Ingredients Official formula Required quantities

1 Iodine 50gm

2 Potassium iodide 100gm

3 Purified water 1000ml 30ml

Calculation:

Procedure:
Dissolve potassium iodide and iodine in a purified water. Shake well till it dissolve. Then add
sufficient purified water to make up the required volume. Filter and transfer in a suitable container.

Use: Antiseptic

Storage: Well closed container made up of iodine resistant material.

Dose: 0.3 to 2ml.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Label:

AQUEOUS IODINE SOLUTION

30ml
Composition:
Iodine
BATCH NO.: MFG. DATE:
Potassium iodide
Purified water
MFG. LIC NO.: EXPIRY DATE:
Category: Antiseptic

FOR EXTERNAL USE ONLY

Use: It is used as an antiseptic
Storage: Stored in a well closed container at a temperature not exceeding 250C
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 3
PREPARATION OF CALAMINE LOTION
Aim: To prepare and submit 30ml of Calamine lotion.
Reference:
Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 165 -166
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, funnel etc.
Chemical Required:
Calamine, Zinc oxide, Bentonite, Glycerin, sodium citrate, Liquefied phenol, rose water,
Purified water.
Principle:
It is a suspension containing in-diffusible Solids for external use. Here we use bentonite as a
suspending agent, dispersion of bentonite is observed if it is mixed intimately with insoluble
medicament i.e. calamine. Zinc oxide used here acts as astringent & protective agent. Sodium citrate
causes partial deflocculation of calamine and transfers bentonite from gel to solid form in its absence.
Hence it is thicker & difficult to pour from bottle.

Formula:

Sl. No. Ingredients Official Required quantities

formula
1 Calamine 150gm
2 Zinc oxide 50gm
3 Bentonite 15gm
4 Glycerine 5ml
5 Sodium citrate 5ml
6 Liquefied phenol 50ml
7 Rose water 1000ml 30ml

Calculation:

Procedure:
Mix the weighed amounts of Calamine, ZnO, Bentonite in a mortar & pestle.
Triturate with a solution of sodium citrate in 5 ml of water.
Add required amounts of liquefied phenol & Glycerine.
Mix well, to this add more of vehicle to produce required volume, mix thoroughly so as to get
uniform preparation. Filter and transfer in a suitable container.
Directions:
Shake well before use. To be applied 2-3 times a day.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Uses:
This is used as astringent.
Used as smoothening agent and gives relief from itching and pain during skin diseases and
infections.
It is also used in ring worm infections.

Storage: Stored in a well closed container dark in a cool place at a temperature not exceeding250C.
Label:
CALAMINE LOTION
30ml
Composition:
Calamine
Zinc oxide BATCH NO. MFG. DATE:
Bentonite
Glycerine
Sodium citrate MFG. LIC. NO.: EXPIRY DATE:
Liquefied phenol
Rose water
Category: Astringent
FOR EXTERNAL USE ONLY
Use: This is used as astringent
Storage: Stored in a well closed container dark in a cool place and temperature not exceeding 250C
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 4
PREPARATION OF SULPHUR OINTMENT

Aim: To prepare and submit 100gm of Sulphur ointment I.P.

Reference:
✓ Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi,2017;238
✓ Pharmacopoeia of India.
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, ointments slab etc.
Chemical Required:
Sublimed Sulphur, wool fat, hard paraffin, white soft paraffin, Cetostearyl alcohol.
Principle:
Ointments are semisolid preparation meant for external application to the skin or mucous membrane.
They usually contain medicament or medicaments which are dissolved or dispensed or suspended or
emulsified as an antiseptic, antifungal agents and Sulphur ointment used to treat different skin
infections.
Formula:
Preparation of simple ointment base:

Sl. No. Ingredients Official formula Required

quantities
1 Wool fat 5gm
2 Hard paraffin 5gm
3 Ceto stearyl alcohol 5gm
4 White soft paraffin 85gm 100gm

Sl. No. Ingredients Official formula Required quantities

1 Sublimed 10gm
Sulphur
2 Simple ointment 90gm 100gm

Calculation:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Procedure:
Preparation of simple ointment:
✓ Required quantity of hard paraffin and Cetostearyl alcohol.
✓ Wool fat and white soft paraffin were incorporated and stirred until all ingredients were melted.
✓ The mixture was stirred thoroughly until cooled.
Preparation of Sulphur ointment:
Sublimed Sulphur was triturated and finely shifted through sieve no.85. Required quantity of Sulphur
was taken on ointment base and mix small amount of ointment of simple ointment was gradually added
until homogeneous mass was obtained.

Direction: Apply as directed.

Use:
✓ This are used to treat acne Sulfur ointment.
✓ This are used to treat seborrheic dermatitis and scabies.

Storage: Keep in well closed container and cool place.

SULPHUR OINTMENT I.P

100gm

Compositions:
Wool fat BATCH NO. MFG. DATE:
Hard paraffin
Cetostearyl alcohol
White soft paraffin MFG. LIC. NO.: EXPIRY DATE:
Category: Acne

FOR EXTERNAL USE ONLY.

Use: This are used to treat acne Sulphur ointment and also used to treat seborrheric dermatitis and
scabies.

Storage: Stored in a well closed container dark and cool place and temperature not exceeding 250C

MFG BY: ABCD Batch: Roll No.:

Label:

Report:
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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 5
PREPARATION OF MAGNESIUM HYDROXIDE MIXTURE

Aim: To prepare and submit 20ml of Magnesium Hydroxide mixture.

Reference:
Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 150-151.
Subrahmanyam C.V.S., Thimmasetty J.J, Prabhushankar G.L., Laboratory manualof Pharmaceutics.
Vallabh Publication, Delhi, 2019; 47-48.
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, funnel etc.
Chemical Required:
Magnesium sulphate, sodium hydroxide, light magnesium oxide and purified water.
Principle:
It is aqueous of colloidal dispersion of magnesium hydroxide. It is prepared by precipitation method
and hydration method or both. It is not excessively thick or thin but holds design viscosity, it has two
actions in low dose (1 -4ml) it acts as an antacid, while in higher dose (8–10ml) it acts as a laxative.
Reaction:
MgO +H2O ------- Mg (OH)2 Mg SO4+ 2NaOH --------------------------Mg (OH)2 + Na2SO4

Formula:

Sl. Ingredients Official formula Required quantities

No.
1 Magnesium sulfate 47.5gm
2 Sodium hydroxide 50.5gm
3 Light magnesium 15gm
oxide
4 Purified water 1000ml 20ml

Calculation:

Procedure:
✓ Dissolve sodium hydroxide in sufficient purified water contained in a beaker.
✓ Triturate Magnesium oxide in a mortar with the solution of hydroxide to from a
smooth cream.
✓ Dissolve magnesium sulphate in sufficient water in a separate beaker.
✓ Add cream to the solution of magnesium sulphate with constant stirring. This mixture is
set-aside for 48 hrs.
✓ After 48 hrs pour off the supernatant liquid.
Add hot purified water to the precipitate of magnesium hydroxide to wash the sulphate ions from
precipitate.
✓ Transfer the washed precipitate to the suitable container and label it.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Dose: 1 to 4ml as antacid, 8 to 10ml as laxative.
Storage: Stored in an air tight container in a cool place.
Direction: Shake well before use
Label:
MAGNESIUM HYDROXIDE MIXTURE
20ml
Composition:
Magnesium sulfate BATCH NO. MFG. DATE:
Sodium hydroxide
Light magnesium oxide MFG. LIC. NO.: EXPIRY DATE:
Purified water
Category: Laxative.
FOR INTERNAL USE ONLY
Use: Laxative & Antacid
Storage: Stored in a well closed container dark in a cool place and temperature not exceeding 250C
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 6
PREPARATION OF TURPENTINE LINIMENT

Aim: To prepare and submit 30ml of Turpentine Liniment.

Reference:
Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017;164 -165
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker, funnel etc.
Chemical Required:
Soft soap, camphor, turpentine oil, purified water.
Principle:
Liniments are solution or suspensions or emulsion intended for external application. They aregenerally
applied with massage. Liniment of turpentine is used externally in a patient suffering fromarthalgia,
myalgia, fibrositis, and sprain.

Formula:

Sl. No. Ingredients Official formula Required quantities

1 Soft soap 9gm

2 Camphor 5gm

3 Turpentine oil 69gm

4 Purified water 1000ml 30ml

Calculation:
Procedure:
✓ Take the required quantity of soft soap in the mortar and add water in thrice the quantity as soft soap.
✓ Triturate to make a soapy solution.
✓ Take the required quantity of oil of turpentine in a dry measure glass and dissolve camphor in it.
✓ Add this solution drop by drop in the mortar and triturate continuously and rapidly till the
primaryemulsion is formed.
✓ Add a small quantity of water and transfer it to the previously calibrated round vertically ribbed
andblue and amber colored bottle.
✓ Adjust to the required volume by adding water, attach the cork and label it.

Use:
Liniment of turpentine is used externally in a patient suffering from arthralgia, myalgia, fibrositis, and
sprain.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Storage:
It should be store in a well closed container dark in a cool place.

Label:

TURPENTINE LINIMENT
30ml
Composition:
Soft soap BATCH NO. MFG. DATE:
Camphor
MFG. LIC. NO.: EXPIRY DATE:
Turpentine oil
Purified water
Category: Arthralgia
FOR EXTERNAL USE ONLY.
Use: Liniment of turpentine is used externally in a patient suffering from arthralgia, myalgia,
fibrositis, and sprain.
Storage: Stored in a well closed container dark in a cool place and temperature not exceeding
250C
MFG BY: ABCD Batch: Roll No.

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 7
PREPARATION OF EFFERVESCENT GRANULES

Aim: To prepare and submit 10gm of Effervescent granules.

Reference:
✓ Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 127
✓ Subrahmanyam C.V.S., Thimmasetty. J, Prabhushankar G.L., Laboratory manual of Pharmaceutics.
Vallabh Publication, Delhi, 2019; 88
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance sieve shaker, China dish etc.
Chemical Required:
Sodium phosphate, sodium bicarbonate, tartaric acid.
Principle:
Effervescent preparation provides effervescence of CO2 gas when added to water by a chemical
reaction between alkali metal carbonates or bicarbonates with tartaric acid or citric acid. This
preparation is intended to be dissolved in water before it is being taken orally. The CO2 gas is released
as a result of acid-base reaction. The preparation is advised to be taken while effervescence.
Formula:
Sl. No. Ingredients Official formula Required quantities

1 Sodium phosphate 0.9gm

2 Sodium bicarbonate 2.1gm
3 Tartaric acid 1.1gm
4 Citric acid 0.7gm 10gm

Calculation:
Procedure:

✓ Citric acid is mixed with sodium phosphate and tartaric acid.

✓ Add sodium bicarbonate to the above mixture and mix it gently.
✓ The final powdered mixture is placed in a China dish previously heated on water bath.
✓ Stir the mixture with the help of a spatula constantly still it becomes damp mass.
✓ Pass damp mass through sieve number 8.
✓ Dry granules at a room temperature not exceeding 540C.
✓ Pass dry granules through appropriate sieve.
✓ Pack dry uniform sized granules in a wide mouthed bottle.

Dose: Each 5g contains sodium phosphate 1g.

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 Storage: Stored in air tight container in a cool place,

Use: Saline purgative and mild diuretic.

Label:

EFFERVESCENT GRANULES
10gm
Composition:
Sodium phosphate BATCH NO. MFG. DATE:
Sodium bicarbonate
Tartaric acid
MFG. LIC. NO.: EXPIRY DATE:
Citric acid
Category: Saline purgative and Mild diuretic
FOR INTERNAL USE ONLY.
Use: Saline purgative and Mild diuretic
Storage: Stored in a well closed air tight container in a cool place and temperature not exceeding
250C
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 8
PREPARATION OF DUSTING POWDER

Aim: To prepare and submit 30gm of Dusting powder.

Reference:
✓ Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 115
Requirements:
Apparatus Required: Mortar & pestle, weigh balance, sieve shaker etc.
Chemical Required: Purified talc, starch, zinc oxide
Principle:
Dusting powders are usually mixtures of two or more substances in fine powder intended for external
use. Starch possess binding as well as good flow property which helps the powder to flow easily,
spread uniformly and cling to the skin on application. Talc is natural mineral substance zinc oxide is
protectant.

Formula:
Sl. No. Ingredients Official formula Required quantities

1 Purified talc 1gm

2 Starch 0.5gm

3 Zinc oxide 0.5gm 30gm

Calculation:

Procedure:
✓ Weigh the required quantity of purified talc and zinc oxide.
✓ Mix zinc oxide with starch and incorporate purified talc.
✓ Mix thoroughly and pass the mixed powder through a sieve no.120 to remove gritty particles.
✓ After sieving whole of the powder must be again slightly mixed.
✓ Pack the powder to protect it from air, moisture and contamination.
Storage: Store in a well closed container and should be kept in a cool place.
Category: Antiseptic dusting powder.

Caution: Do not apply on raw (or) weeping surface.

Direction: For external use only.

Use: Antiseptic.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Label:

DUSTING POWDER
30gm
Composition:
Purified talc BATCH NO. MFG. DATE:
Starch
Zinc oxide MFG. LIC. NO.: EXPIRY DATE:
Category: Antiseptic
FOR EXTERNAL USE ONLY.
Use: Antiseptic
Storage: Store in a well closed container and should be kept in a cool place.
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 9
PREPARATION OF PIPERAZINE CITRATE ELIXIR

Aim: To prepare and submit 20ml of Piperazine citrate elixir.

Reference:
Mehta R.M., Dispensing Pharmacy, Vallabh Prakashan, New Delhi, 2017; 158 - 160
Requirements:
Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod, spatula, beaker etc.
Chemical Required:
Piperazine citrate, chloroform spirit, glycerin, orange oil, syrup, purified water.
Principle:
Piperazine Citrate Elixir is mainly an anthelmintic. This kind of drug works to paralyze parasites that
may invade the host body and cause diseases. It therefore helps to remove the parasites and thus
inhibit the spread of disease in the body. The medicine is used to treat ascariasis,
enterobiasis, also called common pinworms. The drug immobilizes the parasitic worms first, which
are later passed out of the body through stool. This drug can only be obtained with a proper
prescription by your doctor and is not available over the counter. The medicine is available inthe form
a tablet or as syrup.

Formula:

Sl. No. Ingredients Official Formula Required quantities

1 Piperazine citrate 180gm

2 Chloroform spirit 0.5ml
3 Glycerine 100ml
4 Orange oil 0.25ml
5 Syrup 500ml
6 Purified water 1000ml 20ml

Calculation:
Procedure:
Dissolve piperazine citrate in part of water. Then mix with agitation orange oil, glycerin, syrup in
chloroform spirit and pour in watery solution of piperazine citrate. Adjust the volume with sufficient
purified water. Filter and transfer in a suitable container.

Storage:
Stored in a well closed container at a temperature not exceeding 250C.

Use:
It is used as anthelmintic which is used in the treatment of worm infections.

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Label:

PIPERAZINE CITRATE ELIXIR

20ml
Composition:
Piperazine citrate
Chloroform spirit BATCH NO. MFG. DATE:

Glycerin
MFG. LIC. NO.: EXPIRY DATE:
Orange oil
Syrup
Purified water
Category: Anthelmintic
FOR INTERNAL USE ONLY.
Use: It is used as an anti-helminthics are used in the treatment of worm infections.

Storage: Stored in a well closed container dark in a cool place and temperature not exceeding
250C

MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
EXPERIMENT – 10
PREPARATION OF IODINE THROAT PAINT

Aim: To prepare and submit 30ml of Iodine Throat Paint (Mandles Paint)

Reference:
1. Mehta R.M., Dispensing Pharmacy,Vallabh Prakashan,New Delhi,2017;169 - 170
2.Pharmacopoeia of India.

Requirements:

Apparatus Required:
Mortar & pestle, weigh balance, measuring cylinder, glass rod,spatula,beaker,funnel etc.

Chemical Required:
Potassium Iodide, Iodide, alcohol, purified water, peppermint oil, Glycerin.

Principle:
Mandls throat paint is the solution of iodine in glycerin. Iodine and potassium iodide form
complexes such as KI, KII, KII2 KII3.These poly iodides are more soluble in solvents like
water, glycerin and hydro alcoholic mixture. These poly iodides, because of high solubility keeps
iodine in solution. Glycerin is used as a vehicle in preparation to get a prolonged contact of
medicament to the infected surfaces because of its high viscosity. Peppermint oil is used as
flavor the throat paint and to keep it in solution little alcohol is added.

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 Formula :
Sl. No. Ingredients Official formula Required quantities

1 Potasium iodide 5gm

2 Iodine 10.5gm

3 Alcohol 90% 40ml

4 Water 25ml

5 Peppermint oil 4ml

6 Glycerine q.s 1000ml 30ml

Calculation:

Procedure:
Accurately weighed of potassium iodide was accurately dissolved in water and iodine was
added to it. Thesolution was stirred until iodine completely dissolves, then peppermint oil was
added to alcoholin another container. Both the solutions were mixed well make up to volume
with glycerin thenthe throat pain was transferred to a clean wide mouth container.

Use: Mandl’s paint is a throat paint used in the treatment of sore throat

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Storage: Stored in a cool place in a well closed container.

Category: Throat paint.

Special instruction: Shake well before use

Label:

IODINE THROAT PAINT (MANDLES PAINT)

30ml
Composition:
Potasium iodide BATCH NO. MFG. DATE:
Iodine
Alcohol 90%
MFG. LIC. NO.: EXPIRY DATE:
Water
Peppermint oil
Glycerine
Category: Throat paint.
FOR INTERNAL USE ONLY.
Use: Mandl's Paint is a throat paint used in the treatment of sore throat
Storage: Stored in a well closed container at a temperature not exceeding 250C
MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 11
PREPARATION OF ORS POWDER

Aim: To prepare and submit 20gm of ORS powder (WHO)

Reference:
1. Mehta R.M., Dispensing Pharmacy,Vallabh Prakashan,New Delhi,2017;123

2. Subrahmanyam C.V.S.,Thimmasetty .J, Prabhushankar G.L., Laboratory manual of

Pharmaceutics.Vallabh Publication,Delhi,2019; 90

Requirements:

Apparatus Required:
Morter & pastle,weigh balance ,sieve shaker etc.

Chemical Required:
Sodium chloride, glucose, potassium chloride, dry sodium citrate.

Principle:
ORS powder is used as an electrolyte replenisher and usually given in case of severe watery
diarrhea. The quantity of all salts is according to the osmolarity required as recommended by
WHO guidelines. Addition of powders intake geometrical ratio is to obtain uniform powder
mixture. To protect the powder the powder from the effect of atmospheric conditions.

Formula:
Sl. No. Ingredients Official formula Required quantities
1 Sodium chloride 2.6gm
2 Glucose 13.5gm
3 Potassium chloride 1.5gm
4 Tri sodium citrate dihydrate 2.4gm 20gm

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Calculation:

Procedure:
1.Weigh sodium chloride, glucose, potassium chloride, disodium citrate in to the mortar.
2.Mix all the salts by adding them in geometrical ratio
3.Store the powder mixture into a airtight container and label it.

Dose: Each 5g is dissolved in 25oml of purified water.

Use: Electrolyte replenisher.

Storage Keep in an airtight container.

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Label:

ORS POWDER (WHO)

20gm

Composition
: Sodium BATCH NO. MFG.
chloride
Glucose DATE: MFG. LIC. NO.:
Potassium chloride
Tri sodium citrate dihydrate EXPIRY
Category: Electrolyte replenisher.

DATE:

FOR INTERNAL USE ONLY.

Use: Electrolyte replenisher.

Storage: Stored in a well closed airtight container at a temperature not exceeding 250C

MFG BY: ABCD Batch: Roll No.:

Report:

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 12
PREPARATION OF IODINE GARGLE

Aim: To prepare and submit 30ml of Iodine gargle

Reference:

1. Mehta R.M., Dispensing Pharmacy,Vallabh Prakashan,New Delhi,2017;168

2. Subrahmanyam C.V.S.,Thimmasetty .J, Prabhushankar G.L., Laboratory manual of

Pharmaceutics.Vallabh Publication,Delhi,2019; 74

Requirements:

Apparatus Required:
Morter & pastle,weigh balance,measuring cylinder,glass rod,spatula,beaker,funnel etc.

Chemical Required:
Povidone iodine, purified water,

Principle:
It is used to treat infections of lining of mouth and throat such as gingivitis and mouth ulcers. It
is an antiseptic active against micro-organisms, bacteria, fungus, virus, protozoa and bacterio
spores. It is also used for oral hygiene before during after dental or oral surgery. It is used by
diluting with equal volume of warm water gargle up to 10ml for 30 sec without swallowing.
These are supplied in coloured fluted bottles.

Formula:
Sl. No. Ingredients Official formula Required quantities

1 Povidone iodine 1gm

2 Purified water q.s 100ml 30ml

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Calculations:

Procedure:
Weighed 1gm of povidone iodine and transfer to 100ml measuring cylinder and add boil and
cooled purify water to the measuring cylinder, stir well. Transfer the solution in an appropriate
ambered colored glass bottle and label it.

Direction:
Dilute the solution with equal volume of water (warm) Before use. Gargle for 30sec.

Storage:
Store in a cool and dark place.

Use:
Iodine is an antiseptic suitable for use on the skin and mucosa.

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 Label:

IODINE
GARGL
E
30ml
Compositions:
Povidone BATCH NO. MFG.

iodine
DATE: MFG. LIC. NO.:EXPIRY
Purified
water DATE:
Category: Antiseptic

FOR INTERNAL USE ONLY.

Use: Iodine is an antiseptic suitable for use on the skin and mucosa

Storage: Stored in a well closed container cool and dark place and temperature not
exceeding 250C
MFG BY: ABCD Batch: Roll No.:

Report:

29
IQ City Institute of Pharmaceutical Sciences, Durgapur
 EXPERIMENT – 13
PREPARATION OF CHLORHEXIDINE MOUTHWASH

Aim: To prepare and submit 30ml of Chlorhexidine mouthwash

Reference:
1. Mehta R.M., Dispensing Pharmacy,Vallabh Prakashan,New Delhi,2017;169

2. Subrahmanyam C.V.S.,Thimmasetty .J, Prabhushankar G.L., Laboratory manual of

Pharmaceutics.Vallabh Publication,Delhi,2019; 79-80

Requirements:
Apparatus Required:
Morter & pastle,weigh balance,measuring cylinder,glass rod,spatula,beaker,funnel etc.

Chemical Required:
Zinc sulphate, Zinc chloride, Dil HCl, tartrazine solution, chloroform water,

Principle:
Zinc sulphate, Zinc chloride, acts as an astringent and dil HCl is added to form clear solution,
compound tartrazine is a coloring agent and chloroform water acts as a vehicle. This is prepared
by simple solution method.

Formula:
Sl. No. Ingredients Official formula Required quantities
1 Zinc sulphate 20gm
2 Zinc chloride 1gm
3 Dil HCL 1ml
4 Compound tartrazine 1ml
solution
5 Chloroform water q.s 100ml 30ml

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Calculations:

Procedure:
Dissolve Zinc sulphate, Zinc chloride in chloroform water add dil HCL to make clear solution.
Add compound tartrazine solution and finally make up with chloroform water.Add more of
purified water to produce the required volume. Filter and transfer in a suitable container.

Direction: For oral use only

Uses: Astringent

Storage: Store in a well closed container & in cool place

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IQ City Institute of Pharmaceutical Sciences, Durgapur
 Label:

CHLORHEXIDINE
MOUTHWASH
30ml
Compositions:
Zinc
sulphate BATCH NO. MFG. DATE:

Zinc
chloride MFG. LIC. NO.: EXPIRY DATE:
Dil HCL
Compound tartrazine
solutionChloroform water
Category: Astringent
FOR INTERNAL USE ONLY.
Use: It is used as an astringent

Storage: Store in a well closed container & in cool place

MFG BY: ABCD Batch: Roll No.:

Report:

32
IQ City Institute of Pharmaceutical Sciences, Durgapur