QP CODE:1025
Rajiv Gandhi University of Health Sciences, Karnataka
MBBS Phase - I (CBME) Degree Examination - 13-Dec-2024
Time: Three Hours Max. Marks: 100 Marks
BIOCHEMISTRY - PAPER II (RS-4)
QP Code: 1025
(QP contains three pages)
Your answers should be specific to the questions asked
Drawneat labeled diagrams wherever necessary
LONG ESSAYS 2 x 10 = 20 Marks
1. A25-year-old female presented with mild anemia, jaundice and splenomegaly. She had
previous history of frequent blood transfusion. Laboratory investigations shows
spherocyctes (70%), increased reticulocyte count and findings of hemolytic jaundice.
a. Write the serum and urinary findings in a case of hemolytic jaundice.
b. Enumerate the steps in the formation and fate of bilirubin.
C. List two more conditions leading to hemolytic jaundice
2. Enumerate the steps of transcription in prokaryotes. Write the post-transcriptional
modifications.
SHORT ESSAYS 8x 5 =40 Marks
3 An Eight month old child was brought to hospital for frequent episodes of vomiting and
failure to thrive. On examination, the child was drowsy and had hepatomegaly. Laboratory
investigations revealed increased serum ammonia leveis.
a. Mention the transport form of ammonia.
b. Name the urea cycle disorders and write their enzyme defect.
4 The following is the laboratory investigations in a patient who presented with oedema.
Parameter Serum Total Protein Serum Albumin A/G Ratio
Results 5.0 g/dl 2.3 g/dl 0.85:1
a. Mention normal A/G ratio (Albumin/Globulin ratio).
b. Writetwo conditions in which A/G ratio is altered.
C. Write two functions of albumin.
5 Enumerate the steps in the formation of uric acid.
6 Explain the structure of immunoglobulin with a neat labelled diagram.
7 Write the nutritionalclassification of amino acids with examples.
8. Write the steps of polymerase chain reaction (PCR). Mention two applications of PCR.
9. Write five examples of Transmethylation reactions.
10. What are oncogenes and proto-oncogenes? Explain the mechanism of activation of
oncogene.
Page 1 of3
QP CODE:,
Sciences, Karnataka
Rajiv GandhiUniversity of Health
10x3=30 Marks
SHORT ANSWERS
11. Write three features of genetic code.
where gene therapy is applied.
12. What is gene therapy? Mention two conditions
technology and write their importance.
13. Name the vectors used in Recombinant DNA
14. Write the mechanism of action of steroid hormones.
15. Name three pre-analytical variables affecting laboratory results.
16. Write the structure and function of t-RNA.
17. Name three antioxidant enzymes and write their importance.
18. What is clearance test? Why creatinine clearance is preferred over urea clearance test?
19. Write three examples for detoxification reaction by conjugation mechanism.
20. Mention any three biologically important nucleotides and write their importance.
Multiple Choice Questions 10 x 1 = 10Marks
21 i) Which of the following is a non-protein amino acid?
A. Proline
B. Histidine
C. Ornithine
D. Aspargine
21 ii) Transamination of oxaloacetate results in the formation of
A. Aspartic acid
B. Valine
C. Glycine
D. Serine
21 iii) Persons with sickle cell trait shows an increased resistance to
A. Typhoid
B. Diabetes
C. Malaria
D. Cancer
21 iv) Which one of the following compounds is
A. Melanin synthesized from tryptophan?
B. Serotonin
C. Dopamine
D. Epinephrine
21 v) Salvage pathway for purines involves the enzyme
A. PRPP amidotransferase
B. PRPP synthase
C. Xanthine oxidase
D. HGPRTase
Page 2 of 3
DE: QP CODE:1025
ak Rajiv Gandhi University ofHealth Sciences, Karnataka
22 i) The function of restriction endonuclease enzyme is
A. Joining DNA to cloning vector
B. Cleaving DNA at specific sequence
C. Digesting DNA from its ends
D. Aggregating DNA at centromere
22 ii) Second messenger which mobilises intracellular calcium is
A. Phosphatidic acid
B. Diacylglycerol
C. cyclic GMP
D. Inositol triphosphate
22 ii) Biological target for attack by free radicals are all of the following EXCEPT
A. Polyunsaturated fatty acids
B. GlucOse
C. DNA
D. Protein
22 iv) Retinoblastoma (RB) gene is a
A. Proto-oncogene
B. Oncogene
C. Carcinogen
D. Oncosuppressor gene
22 v) Fasting blood sample is preferred for analysis of
A. Lipid profile
B. Liver function test
C. Renal function test
D. Serum electrolytes
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