Documents I used:
https://iwaponline.com/wst/article/81/1/170/72518/Preparation-of-multifunctional-nanocomposite
s
https://iwaponline.com/wst/article/84/7/1594/83745/Removal-of-multiple-metal-ions-from-wastew
ater-by
https://www.orientjchem.org/vol33no4/extraction-heavy-metals-from-contaminated-water-using-c
helating-agents/#:~:text=The%20results%20showed%20that%20the,increasing%20concentratio
n%20of%20chelating%20agents.
By putting this in AI, it extracted the process:
This method involves creating and using simple chemical solutions to collect
heavy metals like copper (Cu), mercury (Hg), and lead (Pb) from water
EDTA Titration for Metal Ion Concentration
This method is useful for estimating total metal ion concentration. EDTA reacts with
metal ions to form stable complexes, as it is a chelating agent.
Materials Needed:
● 0.01 M EDTA solution.
● Buffer solution (pH ~10, often ammonia buffer).
● Eriochrome Black T (EBT) indicator (turns red in the presence of free metal ions,
blue when chelated).
Procedure:
Prepare your EDTA solution by going through the following:
What we need
1. Disodium EDTA dihydrate (Na₂EDTA·2H₂O) – This is the most common form of EDTA
used in labs.
○ Molar mass: 372.24 g/mol.
2. Distilled or deionized (DI) water.
3. A balance – To measure the precise amount of EDTA.
4. A volumetric flask – For accurate measurement of the final solution volume.
5. A stirring rod or magnetic stirrer.
Step-by-Step Procedure
Step 1: Calculate the Mass of EDTA Needed
The formula for calculating the mass of a solute required is:
Mass (g)=Molarity (M)×Volume (L)×Molar mass (g/mol)\text{Mass (g)} = \text{Molarity (M)}
\times \text{Volume (L)} \times \text{Molar mass (g/mol)}Mass (g)=Molarity (M)×Volume
(L)×Molar mass (g/mol)
● For a 0.01 M solution:
○ Molarity = 0.01 M
○ Volume = 1 L (or 1000 mL)
○ Molar mass of EDTA = 372.24 g/mol
Mass=0.01×1×372.24=3.7224 g\text{Mass} = 0.01 \times 1 \times 372.24 = 3.7224 \,
\text{g}Mass=0.01×1×372.24=3.7224g
You need 3.72 g of disodium EDTA dihydrate to prepare 1 L of 0.01 M solution.
Step 2: Measure the EDTA
● Weigh 3.72 g of EDTA using a balance.
● Place it into a clean, dry container.
Step 3: Dissolve the EDTA
● Add about 800–900 mL of DI water to a beaker or flask.
● Stir the solution using a stirring rod or a magnetic stirrer until the EDTA dissolves
completely. EDTA dissolves slowly, so be patient.
Step 4: Adjust the Volume
● Transfer the dissolved solution to a 1 L volumetric flask.
● Rinse the beaker with small amounts of DI water and pour the rinse into the volumetric
flask to ensure no EDTA is left behind.
● Add DI water to the volumetric flask until the bottom of the meniscus aligns with the 1 L
mark.
Step 5: Mix the Solution
● Stopper the flask and invert it several times to mix thoroughly.
Storing the Solution
1. Label the container as 0.01 M EDTA Solution.
2. Store in a cool, dry place. It can typically be stored for several months if kept sealed and
uncontaminated.
Main Experiment:
3. Prepare the Sample:
○ Adjust the pH of your treated water sample to ~10 using the buffer
solution.
4. Add Indicator:
○ Add 2–3 drops of EBT indicator to the sample. The solution will turn red.
5. Titrate with EDTA:
○ Slowly add EDTA solution from a burette while swirling the sample.
○ Stop when the color changes from red to blue, indicating all metal ions are
chelated.
6. Calculate the Concentration:
○ Use the formula: Metal ion concentration
Advantages:
● Works for total metal ion concentration.
● Requires only basic lab equipment.
