Entrance examination for grade 12 from 1995 – 2011
Grade 11 Unit 3
Enzymes
1, Select the enzyme which has wide application in leather industry to remove hairs and make the
leather product flexible
A, Rennin B, Lipase C, Lactose B, Trypsin
2, which class of enzymes joins molecules together?
A, Lyases B, Hydrolases C, Transferases D, Ligases
3, Which of the following mixtures the enzymes catalyze acts faster on hydrogen peroxide?
A, H2O + HCl + fresh potato cube
B, H2O2 + NaOH + fresh potato cube
C, H2O2 + fresh potato cube
D, H2O2 + boiled potato cube
4, To which of the following organic molecules enzymes belong?
A, Carbohydrate B, Lipid C, Protein D, Nucleic acid
5, Which of the following enzymes works best in acidic PH?
A, Pepsin B, Salivary amylase C, Pancreatic lipase D, Catalase
6, Which enzyme is used in textile industry to remove starch used in fabrics as adhesive or support
during weaving?
A, Amyloglucosidase B, Lipase C, Amylase D, Trypsin
7, To which one of the following is the origin of the term enzyme more related ?
A, Yeast B, Bacteria C, Virus D, Protozoa
8, Which of the following is effective to reverse enzyme inhibition that is caused by reversible
competitive inhibitor? It is to increase:
A, The PH of the reaction B, The substrate concentration
C, The temperature of the reaction D, The concentration of the inhibitors
9, Which of the following statement is true?
A, Enzymes do not start chemical reaction
B, Enzymes lower the activation energy of the chemical reaction
C, Enzymes are used up during reaction they catalyze
D, All of the above
10, Which of the following enzymes has its optimum PH in the acidic range?
A, Catalase B, Pancreatic lipase C, Salivary amylase D, Pepsin
11, Which part of human sperm cell contains the respiratory enzymes ?
A, head B, Middle piece C, Neck D, Tail
12, The enzyme that has wide industrial application in starch industries, textile industries and in the
making of biological detergents is
A, trypsin B, Lipase C, Amylase D, isomerase
13, If an enzyme has optimum activity at neutral PH, to which of the following PH values does this
correspond?
A, 14 B, 7 C, 12 D, 4
14, What is true about the rate of an enzyme catalyzed reaction if the enzyme concentration is kept
constant and the substrate is supplied in excess? The rate would be
A, Rise continuously B, Stay constant C, Decline sharply D, None of the above
15, Among the following identify the molecule that is an enzyme?
A, starch B, cellulase C, Cellulose D, Glycogen
16, An enzyme known as catalase convert hydrogen peroxide into water and oxygen, in this reaction
which one is the substrate?
A, Catalase B, Hydrogen peroxide C, Water and oxygen D, water only
17, Identify the statement that more correctly explains why each cell contains so many different types of
enzymes? Enzymes are:
A, consumed in the process of catalysis B, Used over and over again
C, Specific to their substrates D, Easily synthesized in the cells
18, If an enzyme is provided with its normal substrate plus another molecule having the same shape and
size as substrate, which one of the following would happen?
A, Allosteric inhibition B, competitive inhibition C, Irreversible inhibition D, end product inhibition
19, If one keeps on adding substrate to a fixed amount of an enzyme, when does the reaction rate reach
a plateau?
A, when all substrate is converted to product
B, when all the active sites of the enzymes are occupied
C, when most of the enzyme molecules are consumed
D, when much of the products are accumulated
20, What do we call substance upon which enzymes act?
A, Product B, Substrate C, Activation energy D, Enzyme substrate complex
21, Which factor has more negative effect on the functions of enzymes than the others?
A, Neutral PH B, Very high temperature
C, Optimum amount of salt concentration D, Optimum amount of substrate concentration
22, When an enzyme is denatured by heat or extreme PH, which one of the following does it lose?
A, Peptide bond B, Primary structure C, Secondary structure D, Tertiary structure
23, Which of the following mechanisms do cells use to regulate enzyme catalyzed reaction in metabolic
pathway?
A, Enzyme denaturation B, Irreversible inhibition
C, End product inhibition D, Competitive inhibition
24, In which one of the following points does the induced fit model of enzyme action differ from thee
lock and key model?
A, Enzymes lower the energy of activation
B, Substrate bind at the active site of the enzyme
C, During the reaction, an enzyme substrate complex formed
D, The shape of substrate and active site are complementary
25, suppose 25% of molecules of an enzyme are inhibited by non-competitive inhibitor, which one of the
following would happen is the amount of substrate increased by 50%?
A, the reaction rate would double
B, More enzyme molecules would get inhibited
C, The rate of reaction would decrease by 50%
D, The rate of reaction would remain unchanged
26, In competitive inhibition, which one of the following factors determines the rate of inhibition?
A, The reaction temperature B, The enzyme concentration
C, The substrate concentration D, The ratio of inhibitor to enzyme concentration
27, What are the environmental advantages of using enzyme in industry?
A, It makes high production possible with less input of heat
B, It makes high production possible with high output of heat
C, it makes high production with high emission of more CO2
D, It helps high production with supply of more heat and emission of more CO2
28, Which of the following unit is the building unit of an enzyme molecule?
A, Amino acids B, glucose C, nucleotides d, fatty acid
29, Which one of the following classes of enzymes digests carbohydrates?
A, Amylases B, Lipases C, Proteases D, Nucleases
30, To which class of enzymes do the digestive enzymes belong?
A, Esterase B, Transferees C, Hydrolases D, Isomerizes
31, Which of the following types of enzymes inhibition can be removal when the end product of the
metabolic pathway is depleted?
A, Allosteric inhibition B, Non reversible inhibition
C, Competitive inhibition D, Reversible competitive inhibition
32, In case of enzymatic chemical reaction, what do you call the substance that is acted upon by an
enzyme?
A, Polypeptide B, coenzyme C, Vitamin D, Substrate
33, Which of the following is the common characteristics of all enzymes?
A, They act inside the cell only B, They are proteins
C, They require cofactors D, They operate at any PH
34, In to which of the following organic compounds can lipase, maltase an sucrose grouped?
A, Hormones B, carbohydrates C, Nucleic acids D, Enzymes
35, Which of the following PH represents strongest base?
A, 2 B, 7 C, 13 D, 15
36, Which group of organic compounds include enzymes?
A, Protein B, Lipids C, Starch D, Carbohydrates.
37, For biological detergents to effectively remove oily and greasy dirt, which of the following enzymes
should it contain?
A, Cellulase B, protease C, Amylase D, Lipase
38, Which region of human digestive tract contains enzymes that perform well at low PH?
A, Mouth B, Stomach C, Small intestine D, Large intestine
39, To which of the following organic molecules do enzymes belong?
A, Carbohydrates C, Amino acids C, Proteins D, Lipids
40, Which of the following substances has a shape which is similar to that of the substrate of an
enzyme?
A, The reaction product B, The competitive inhibitors ? C, A cofactor D, An allosteric inhibitors
41, Which one of the following terms refers to how fast an enzyme acts on its substrate?
A, Turn over number B, substrate number C, Enzyme number D, Product number
42, The optimum temperature of enzymes found in thermophilic bacteria is
A, Lower than for the enzyme in the human body
B, higher than the enzymes in the human body
C, the same as the enzymes found in the human body
D, lower than the enzymes found in the warm blooded animals
43, Which of the following properties of enzymes makes it possible that single enzyme molecule can act
on many substrates is added to the reaction, which of the following might be the reason?
A, Saturation of the enzyme B, Inactivation of the enzyme
C, Inhibition of enzyme substrate complex formation D, Loss of substrate specificity by the enzyme
44, Which part of human alimentary canals contains digestive enzymes that function at acidic PH?
A, Stomach B, Mouth C, esophagus D, Small intestine
45, Which of the following is made up of globular protein?
A, Enzyme B, Keratin C, Collagen D, glycogen
46, Which of the following industries can reduce more CO2 emission by shifting to the use of enzymes in
manufacturing process?
A, Bread making B, cheese making C, Leather making D, Manufacturing cosmetics
47, In which are of enzyme application is invertase injection to sucrose paste in order to produce liquid
chocolate?
A, detergent making industry B, pulp and paper industry
C, Pharmaceutical industry D, Food processing industry
48, Which one of the following functions best at higher optimum PH than all the rest?
A, Pepsin B, Salivary amylase C, trypsin D, enzyme in the stomach
49, Which class of enzymes joins to molecules together by formation of new bond?
A, Ligase b, isomerase C, lyase D, hydrolase
50, In an enzyme that contains non protein organic molecules, in addition to protein component, what is
the protein component called?
A, Apo enzyme B, Coenzyme C, holoenzyme D, Cofactor
51, Which of the following should be done in order to remove an enzyme inhibition caused by enzyme
inhibitors?
A, Remove affected enzyme molecules B, Add more substrate to the system
C, Remove the end product of the reaction D, Add more inhibitors to the system
52, Deficiency of which of the following human diet is likely to result in deficiency of some coenzymes
like FAD?
A, Essential amino acids B, Vitamins C, Carbohydrates D, Saturated fatty acids
53, What causes tomato fruit to ripen much more slowly when kept in a refrigerator than if left on table
at room temperature?
A, Low temperature slows the normal action of ripening enzymes
B, enzymes produced by bacteria normally inhibits ripening
C, Humidify accelerates enzyme activity and ripening process
D, Normal temperature arrests the action of ripening enzymes
54, What is immediate compound that is formed during an enzyme catalyzed reaction?
A, Reactant B, substrate C, Product D, enzyme substrate complex
55, Which of the following substance can be used over and over again in an enzyme catalyzed reaction?
A, Enzyme substrate complex B, Substrate C, Enzyme D, Reaction product
56, To produce glucose from starch, which of the following enzymes would one use?
A, Isomerases B, lyases C, Hydrolases D, Ligases
57, Among the following factors that affect enzymes activity, which one does not cause enzyme
denaturation?
A, High PH B, Low PH C, High temperature D, Competitive inhibitors
58, Most coenzymes are derivatives of which of the following nutrient?
A, Protein B, Vitamin C, Carbohydrates D, Fats
59, Which class of enzyme inhibitors has similar shape as that of the normal substrate?
A, Allosteric B, competitive C, denaturing D, irreversible
60, Which of the following statements about the enzymes is not correct?
A, Enzymes speed up a chemical reaction
B, Enzymes are very specific in their action
C, The presence of an enzyme will change the outcome of a reaction
D, Enzymes lower the energy of activation for a reaction to occur
61, How does a heavy metal ion reduce the reaction rate of enzymes?
A, By destroying the 3- dimension shape of enzyme active site
B, By raising the temperature above the optimum level for the enzyme
C, By dropping the PH well below the optimum level for the enzyme
D, By inducing mutation that disrupts the functioning of the enzyme
62, What happens when enzymes become denatured?
A, They can normally catalyze the reaction
B, Their active sites are changed
C, The rate of a reaction increases
D, Bonds holding amino acids are unaffected
63, Which type of enzyme catalyzes the joining of two molecules by the formation of new bonds?
A, Decarboxylase B, Dehydrogenase C, Ligase D, oxidoreductase
64, Which of the following can reverse an enzyme inhibition that is caused by allosteric inhibitors?
A, adding more substrate B, depleting thee end product
C, adding more end product D, depleting the substrate
65, What is an apoenzyme?
A, A protein molecule B, Am active enzyme
C, A non-protein molecule D, A non-protein cofactor
66, Which of the fooling is not characteristic of an enzyme?
A,, It is globular protein B, It functions as catalyst
C, It lower energy of activation D, It is converted to product
67, What does an enzyme do to a chemical reaction that allows it to proceed optimally?
A, It lowers the required activation energy
B, It increases the amount of the substrate
C, it increases the required activation energy
D, It modifies both the PH and temperature
68, Which of the following is incorrect with respect to enzyme’s ability to catalyze a reaction?
A, An enzymes provides a reaction surface and hydrophilic environment for the reaction to take place
B, An enzyme binds reactants such that they are positioned correctly and can attain their transition state
configuration
C, An enzyme allows the reactions to for through a more stable transition state than would normally be
the case
D, an enzyme can weaken bonds in reactants through her binding process
69, In metabolic pathway (A→ B→ C→ D), where each step is catalyzed by a separate enzyme, which
substrate in the pathway serves as an allosteric regulator?
A, Substance A B, Substance B C, Substance C D, Substance D
70, In which industrial enzyme technology is lactase practically applied to produce lactose free milk for
use by lactose intolerant individuals?
A, Chocolate manufacturing industry
B, Human hormone manufacturing industry
C, Dairy product manufacturing industry
D, Cereal food manufacturing industry
71, Which kind of enzyme inhibition binds to enzymes only weakly and the bond that holds them breaks
easily releasing the inhibitors to allow the enzyme to become active again?
A, Irreversuble3 inhibitors
B, reversible inhibitors
C, Inhibitors that bind strongly to enzymes
D, Inhibitors that permanently alters enzyme structure
72, Choose the one in which the human digestive enzyme is incorrectly matched with the substrate that
is normally catalyzes?
A, Lipase- fat B, Pepsin – protein C, Trypsin – starch D, Pancreatic amylase – starch
73, Significant change in PH that can affect an enzyme molecule include
A, Building strong ionic bonds leading to stabilization of enzymes
B, altering the changes on some substances making I difficult to bind to enzyme
C, Changing the color and palatability of food item
D, Increasing the boiling temperature of the substance
74, What is the shape of enzyme molecule?
A, Linear B, pleated C, branched D, globular
75, Which of the following condition is least likely to denature an enzyme?
A, A low temperature B, An extreme PH
C, heavy metal ions D, A high temperature
76, Which model of enzyme actions requires that the shape of the substrate molecule is complementary
to that of the active site?
A, The induced fit model B, The lock and key model
C, The activation energy model D, The enzyme function model
77, The industrial use of enzymes is helpful for the environment as it contributes to reduction of CO2
emission. In which of the following is there more mass of CO2 emission saved per ton of products by
using enzyme technology rather than traditional method?
A, Cheese making b, Manufacture of cosmetics C, Paper making D, Bread making
78, Which of the following is not correct about the effect of temperature on enzymes?
A, Raising the temperature increases the kinetic energy of the molecule
B, Most enzymes are insensitive to temperature at low PH
C, A ten degree centigrade increase in temperature may increase the activity of most enzymes by 50% to
100%
D, Most enzymes are denatured at 40 degree centigrade
79, Which of the following can reverse or reduce an allosteric enzyme inhibition?
A, Adding more substrate B, Adding more end product
C, Depleting the end product D, Deleting the substrate
80, Which of the following factors affects enzymes activity without denaturing the enzyme molecule?
A, Low temperature B, High temperature
C, High PH D, low PH
81, In human body, which conditions are generally considerd optimum for most enzymes?
A, 37 degree centigrade and PH 1.0 B, 37 degree centigrade and PH 7.0
C, 5 degree centigrade and PH 9.0 D, 90 degree centigrade and PH 7.0
82, Which of the following statements best describes an induced fit model?
A, The substrate binds to an active site and alters the shape active site
B, The substrate adopts the correct binding conformation before entering an active site
C, An active site alters shape such that it is ready to accept a substrate
D, An active site alters the shape of the substrate such that it can adopt the necessary active
conformation for binding
83, If conditions were remain constant, which one of the following changes would explain a reduced
rate of activity in an enzyme controlled reaction? Increase in
A, Enzyme concentration
B, substrate concentration
C, Concentration of end product
D, Temperature toward the optimum
Answer
No. Answer No. Answer No. Answer No. Answer
1 D 26 C 51 B 76 B
2 D 27 A 52 B 77 D
3 B 28 A 53 A 78 B
4 C 29 A 54 D 79 B
5 C 30 C 55 C 80 A
6 C 31 A 56 C 81 B
7 A 32 D 57 D 82 B
8 B 33 B 58 B 83 D
9 B 34 D 59 B 84
10 D 35 C 60 C 85
11 B 36 A 61 A 86
12 A 37 B 62 B 87
13 B 38 B 63 C 88
14 B 39 C 64 A 89
15 B 40 B 65 A 90
16 B 41 A 66 B 91
17 C 42 B 67 A 92
18 B 43 A 68 D 93
19 B 44 A 69 D 94
20 B 45 A 70 C 95
21 B 46 D 71 B 96
22 D 47 D 72 C 97
23 C 48 C 73 A 98
24 C 49 A 74 D 99
25 A 50 A 75 C 100
