3M Confidential: 3M™ Attest™ Auto-Reader Service Manual Models 390, 390G, 490, 490H

3M Confidential Manual-Service-05-207096
390/390G/490/490H Auto-reader
3M™ Attest™ Auto-reader Service Manual Service Manual
Models 390, 390G, 490, 490H Version: 3
Status: Release
Release Date:4/15/2015 8:15:12 PM
Document Name: Manual-Service-05-207096
Document Title: 390/390G/490/490H Auto-reader Service Manual
Effective From: 4/15/2015 8:15:12 PM To:
Owner: Schenk Stephen B, A1GY7ZZ
Location Name Description

3M OAKDALE-3MUS-MN 3M 3M OAKDALE-3MUS-MN 3M CENTER - 01SZ6200: 3350
CENTER - 01SZ6200 GRANADA AVE N STE 200, OAKDALE, MN, US
SIGNERS:
Signer Role Date Signed

A441CZZ:Mortensen Matt S Regulatory April 14, 2015 11:06:56 AM
co017755:Sanchez Castillo Juliana Marketing April 15, 2015 08:15:10 PM
A2AFTZZ:Herrlein Chad Quality April 13, 2015 12:52:01 PM
A2C6LZZ:Jennen Anthony J Laboratory April 14, 2015 07:57:27 AM
us332550:Flynn Susan D Technical Services April 13, 2015 11:40:06 AM
Status: Release
Release Date:4/15/2015 8:15:12 PM
APPENDIX
eMatrix Medical Vault
Product/Part Relationships to Document
Product Name Type Description Vault

390 Model Number 390 ATTEST Infection Prevention
AUTOREADER FOR Division
STEAM
390G Model Number 390G ATTEST Infection Prevention
AUTOREADER FOR EO Division
490 Model Number Attest Auto-Reader Infection Prevention
Division
490H Model Number 490H Attest Auto-reader Infection Prevention
Division
Part Name Description State Vault

70-2007-6365-7 490 ATTEST AUTO-READER Release Infection Prevention
FOR ATTEST SUPER RAPID Division
BLS - **NO GRAPHICS
REQUIRED**
70-2007-7180-9 390 ATTEST AUTOREADER Release Infection Prevention
FOR STEAM **NO GRAPHICS Division
REQUIRED**
70-2007-7181-7 390G ATTEST AUTOREADER Release Infection Prevention
FOR EO **NO GRAPHICS Division
REQUIRED**
70-2007-7604-8 490H ATTEST AUTO-READER Release Infection Prevention
FOR H202 BIOLOGICAL Division
INDICATORS **NO GRAPHICS
REQUIRED**
70-2007-8708-6 390 ARGENTINA ATTEST Release Infection Prevention
AUTOREADER FOR STEAM Division
**NO GRAPHICS REQUIRED**
70-2007-8709-4 390G ARGENTINA Release Infection Prevention
AUTOREADER FOR EO **NO Division
GRAPHICS REQUIRED**
70-2007-8710-2 490 ARGENTINA ATTEST Release Infection Prevention
AUTOREADER FOR ATTEST Division
SUPER RAPID BIs **NO
GRAPHICS REQUIRED**
70-2007-8775-5 490H ARGENTINA ATTEST Release Infection Prevention
AUTOREADER FOR Division
HYDROGEN PEROXIDE BIs
**NO GRAPHICS REQUIRED**
Status: Release
Release Date:4/15/2015 8:15:12 PM
Project Name Description State Vault

14313-Rapid BI for H202 14313-Rapid BI for H2O2 Active Infection Prevention
Sterilization Sterilization Division
875-Next Generation BI 875-Next Generation BI Active Infection Prevention
Division
PROJ-05-163847 390 Attest Rapid BI Incubator and Active Infection Prevention
Reader Platform Division
eMatrix Document Relationships
Document Name Description Revision

C-05-158277 3M Attest Auto-reader 390, 390G, 490 and 490H Final 11
Acceptance Specification
DS-INPUT-05-171968 Design Requirements Specifications and Device Specifications 4
for Attest 390 and 390G Rapid BI Autoreaders.
LAB-SUPPORT-05-187032 Attest 390, 390G, 490 and 490H Auto-reader MTS Tool 3
Specification
Supporting Documents
Status: Release
Release Date:4/15/2015 8:15:12 PM
3M™ Attest™ Auto-reader

Service Manual
Model 390 Model 390G
Model 490 Model 490H
Neither this book nor any part may be reproduced or transmitted in any form or by any means, electronic or mechanical, including
photocopying, microfilming, recording or by an information storage and retrieval system, without permission from 3M HealthCare.
© 3M 2015. All rights reserved.

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CONTENTS
Preface .....................................................................................................................................iii
1.0. Specifications.................................................................................................................. 1-1
2.0. Product Description / Intended Use................................................................................. 2-1
3.0. Safety Warnings and Cautions ........................................................................................ 3-1
3.1. Device Safety Compliance ........................................................................................ 3-3
3.2. EMC Compliance ...................................................................................................... 3-4
3.3. Explanation of Product and Packaging Label Symbols.............................................. 3-5
4.0. Warranty and Service Information ................................................................................... 4-1
5.0. Installation and Operation .............................................................................................. 5-1
5.1. Initial Setup ............................................................................................................... 5-1
5.2. Description of Front Panel ......................................................................................... 5-2
5.3. Instructions for Use ................................................................................................... 5-3
6.0. Principles of Operation.................................................................................................... 6-1
6.1. Systems Overview – 3M™ Attest™ Auto-reader ....................................................... 6-1
6.2. Auto-reader Component Description ......................................................................... 6-3
7.0. Maintenance ................................................................................................................... 7-1
8.0. Trouble Shooting Guide .................................................................................................. 8-1
8.1. Caution Codes........................................................................................................... 8-2
8.2. Error Coders.............................................................................................................. 8-4
8.3. Other Service Conditions......................................................................................... 8-10
9.0. Disassembly / Assembly Instructions ............................................................................. 9-1
10.0. Manufacturing Test System (MTS) Setup and Use...................................................... 10-1
10.1. Equipment Required.............................................................................................. 10-1
10.2. MTS Software........................................................................................................ 10-2
10.2.1. Intended Use ................................................................................................ 10-2
10.2.2. MTS Software Description ............................................................................ 10-3
10.2.3. MTS Software Installation ............................................................................. 10-5
10.2.4. Auto-reader Setup for Testing....................................................................... 10-6
10.2.5. MTS Logon and Connection ......................................................................... 10-9
10.3. File Path Preferences .......................................................................................... 10-11
10.4. Fluorescent Calibration Standards Management ................................................. 10-12
10.5. Firmware and Webpage Upgrades ...................................................................... 10-15
10.6. Webpage Upgrades............................................................................................. 10-20
10.7. Firmware Recovery ............................................................................................. 10-22
10.8. Test Descriptions and Procedures....................................................................... 10-25
10.8.3. Calibration Tests......................................................................................... 10-26
10.8.4. Verification Tests ........................................................................................ 10-35
10.9. Serial Number Update ......................................................................................... 10-51
11.0 Mechanical Drawings and Parts List............................................................................. 11-1
11.1 Final Assembly....................................................................................................... 11-1
11.2 BI Block/PCB Assembly ......................................................................................... 11-3
11.3 Calibration Standards............................................................................................. 11-5
12. Electrical Schematics ..................................................................................................... 12-1
12.1. Main Board Schematic .......................................................................................... 12-1
12.2. Sensor Board Schematic....................................................................................... 12-4
12.3. LED Board Schematic ........................................................................................... 12-5

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PREFACE
Serial Number
Each 3M™ Attest™ Auto-reader has a unique 6-digit serial number (e.g. 300180) printed on the
serial label found on the bottom of the unit. The first digit indicates the Auto-reader model (1 =
490, 2= 490H, 3 = 390, 4 = 390G).
Content Disclaimer
Sample printouts, graphics, displays and screens are for information and illustration purposes
only and shall not be used for clinical or maintenance evaluations. Data shown in sample
printouts and screens do not reflect actual names or test results.
Hardware Disclaimer
The 3M™ Attest™ Auto-reader Models 390/390G/490/490H hardware specifications are subject
to change. Any changes or modifications to the authorized system installation have been
verified as compatible with the functionality outlined in this document.
(Note: The 3M™ Attest™ Auto-reader Model 390, 3M™ Attest™ Auto-reader Model 390G,
3M™ Attest™ Auto-reader Model 490 and 3M™ Attest™ Auto-reader Model 490H are
separate devices. They will generically be referred to as 3M™ Attest™ Auto-reader or Auto-
reader in this document due to the similarity in the design and construction. Where there are
differences between devices, the differences will be noted and explained.)
All devices
The Auto-reader is designed to be used only with the Power Supply module and Ethernet cable
supplied by 3M.
Each biological indicator type (BI) is indicated for use with specific Auto-reader models. They
are not compatible with other devices and cannot be used.

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1.0 SPECIFICATIONS
Power Input
Power Supply Operating Condition Units
Voltage Range 100-240 Volts AC
Frequency 50/60 Hertz
Current 0.6 Amps
Environmental Operating Conditions

Auto-reader
Environmental Condition Operating Condition Units
390 390G 490 490H
Altitude 3000 (max) Meters X X X X
Operating Temperature 16 - 29 °C X
16 - 40 °C X X X
Storage Temperature -10 - 50 °C X X X X
Operating Relative Humidity 20-80 (non-condensing) % X X X X
Voltage Range 12 Volts DC X X X X
Frequency DC X X X X
Current 1.2 Amps X X X X
Installation/Over Voltage Category II X X X X
Pollution Degree 2 X X X X
Shipping Information
Condition Specification Units
Unit Dimensions 22.3 W x 23.2 D x 8.3 H cm
Package Size 32.3 W x 31.1 D x 21.0 H cm
Unit Weight 1.0 kg
Unit Weight (w/Power Supply) 1.2 kg
Shipping Weight 2.3 kg

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2.0 PRODUCT DESCRIPTION / INTENDED USE
3M™ Attest™ Auto-reader Model 390

The 3M™ Attest™ Rapid Readout Biological Indicator System provides rapid, reliable
monitoring of steam sterilization processes. The system is comprised of biological indicators,
challenge packs, and an incubator/reader (3M™ Attest™ Auto‑reader 390) with embedded
software.
The 3M™ Attest™ Auto‑reader 390 is designed to incubate and automatically read 3M™
Attest™ Rapid Readout Biological Indicators 1291 for Steam at 60°C for a final fluorescence
result at 1 hour and 3M™ Attest™ Rapid Readout Biological Indicators 1292 and 1292E for
Steam at 60°C for a final fluorescent result at 3 hours. The 3M™ Attest™ Rapid Readout
Biological Indicator 1292E is not cleared for use in the United States.
The 3M™ Attest™ Auto‑reader 390 is also designed to allow for further incubation of 3M™
Attest™ Rapid Readout Biological Indicators for Steam, catalog numbers 1291 and
1292/1292E, for a visual pH color change result at 24 hours and 48 hours, respectively. In the
case of a positive control BI, a yellow color change of the growth media will appear within 24
hours for 1291 BIs and within 48 hours for 1292 BIs. In the case of a processed BI, a media
color change from purple to yellow indicates a steam sterilization process failure. A negative pH
color change result, i.e., media remains purple, can be assessed at 24 hours for 1291 BIs and
at 48 hours for 1292 BIs. This further incubation is optional and not intended for routine use.
A positive fluorescence reading or a visual pH color change indicates a steam sterilization

process failure. However, due to the high sensitivity of the fluorescent result, there is no
advantage to continuing to incubate processed 3M™ Attest™ Rapid Readout Biological
Indicators once the fluorescent result has been documented.
Model 390

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3M™ Attest™ Auto-reader Model 390G

The 3M™ Attest™ Auto‑reader 390G is designed to incubate and automatically read the 3M™
Attest™ Rapid Readout Biological Indicator 1294 for Ethylene Oxide at 37°C for a final
fluorescent result at 4 hours. Due to the high sensitivity of the fluorescent result, there is no
advantage to incubating the 3M™ Attest™ Rapid Readout Biological Indicators 1294 beyond 4
hours.
3M™ Attest™ Rapid Readout Biological Indicators 1294 may be further incubated for a visual
pH color change but they must be transferred to a humidified incubator to avoid media dry out.
In the case of a positive control BI, a yellow color change of the growth media will appear within
7 days. In the case of a processed BI, a media color change from blue‑green to yellow indicates
a sterilization process failure. A negative pH color change result, i.e., media remains
blue‑green, can be assessed at 7 days. This optional further incubation is not intended for
routine use but may be used for special studies.
Model 390G

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3M™ Attest™ Auto-reader Model 490

The 3M™ Attest™ Super Rapid Readout Biological Indicator System provides rapid, reliable
monitoring of steam sterilization processes. The system is comprised of biological indicators,
challenge packs, and an incubator/reader (3M™ Attest™ Auto‑reader 490) with embedded
software.
The 3M™ Attest™ Auto‑reader 490 is designed to incubate and automatically read 3M™
Attest™ Super Rapid Readout Biological Indicators 1491 at 56°C for a final negative fluorescent
result at 30 minutes and 3M™ Attest™ Super Rapid Readout Biological Indicators 1492V at
56°C for a final negative fluorescent result at 1 hour.
The 3M™ Attest™ Auto‑reader 490 is also designed to allow for further incubation of 3M™
Attest™ Super Rapid Readout Biological Indicators, catalog numbers 1491 and 1492V, for a
final visual pH color change result at 24 hours and 48 hours, respectively. In the case of a
positive control BI, a yellow color change of the growth media and/or Nonwoven Material will
appear within 24 hours for 1491BIs and within 48 hours for 1492V BIs. In the case of a
processed BI, a media and/or Nonwoven material color change from purple to yellow indicates a
steam sterilization process failure. A negative pH color change result, i.e. media and Nonwoven
Material remain purple/blue, can be assessed at 24 hours for 1491 BIs and at 48 hours for
1492V BIs. This further incubation is optional and not intended for routine use.
A positive fluorescent reading or a visual pH color change indicate a steam sterilization process
failure. However, due to the high sensitivity of the fluorescent result, there is no advantage to
continuing to incubate processed 3M™ Attest™ Super Rapid Readout Biological Indicators
once the fluorescent result has been documented.
Model 490

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3M™ Attest™ Auto-reader Model 490H

The 3M™ Attest™ Rapid Readout Biological Indicator System for Vaporized Hydrogen Peroxide
Sterilization provides rapid, reliable routine monitoring of hydrogen peroxide vapor sterilization
processes. The system is comprised of a biological indicator and an incubator/reader (3M™
Attest™ Auto‑reader 490H) with embedded software.
The 3M™ Attest™ Auto‑reader 490H is designed to incubate and automatically read 3M™
Attest™ Rapid Readout Biological Indicators 1295 at 60°C for a final fluorescent result at 4
hours.
The 3M™ Attest™ Auto‑reader 490H is also designed to allow for further incubation of positive
control 3M™ Attest™ Rapid Readout Biological Indicators 1295 for a final positive visual pH
color change result at 24 hours. It is recommended that processed BIs be transferred to a
humidified incubator for further incubation. A final negative visual pH color change result is
assessed after 7 days. This further incubation is optional and not intended for routine use.
A positive fluorescent reading or a positive visual pH color change result indicates a sterilization
process failure. However, due to the high sensitivity of the fluorescent result, there is no
advantage to continuing to incubate processed 3M™ Attest™ Rapid Readout Biological
Indicators 1295 once the fluorescent result has been documented.
Model 490H

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3.0 SAFETY WARNINGS AND CAUTIONS

The 3M™ Attest™ Auto-reader and its related devices and accessories are designed to provide
safe and reliable service when used according to the instructions provided. Please read,
understand, and follow all safety information contained in the instructions for use included with
the 3M™ Attest™ Auto -reader and the 3M™ Attest™ Biological Indicator devices prior to use.
If this product is used in a manner not specified, the protection provided by the product may be
impaired.
Explanation of Single Word Consequences
Warning: Indicates a hazardous situation, which, if not avoided, could result in death
or serious injury.
Caution: Indicates a hazardous situation, which, if not avoided, could result in minor or
moderate injury.
The following warnings and precautions should be followed to avoid unsafe actions that could
result in personal injury or damage to the instrument.
Warnings and Safety Precautions
WARNING: To reduce the risk associated with hazardous voltage.

Use indoors only.
Do not use the equipment if it is not working properly or if it has suffered any damage.
Use only the power supply specified for this product and certified for the country of use.

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CAUTION: To reduce the risk of injury or instrument damage.

Do not spill liquid onto or into the instrument. Do not immerse the unit in liquid.
Always unplug the Auto-reader and allow to cool before cleaning.

Clean external surfaces using only the instructions provided by the manufacturer.
Do not open the instrument housing – there are no user serviceable parts.
The instrument must be returned to the manufacturer for repair.

Allow the 3M™ Attest™ Biological Indicator to cool for the recommended time period before
activating. Activating or excessive handling of the biological indicator before cooling may
cause the glass ampoule to burst.
Wear safety glasses when activating the 3M™ Attest™ Biological Indicator
WARNING: To reduce the risk associated with incorrect results.

Instrument is to be used by operators familiar with the device, device functionality, and
Operator’s Manual.
Do not place the instrument in environment exposed to sunlight or strong incandescent light.
Do not place instrument close to any device that emits a strong electro‑magnetic field.
Do not use on a vibrating surface.
Match the cap color of the 3M™ Attest™ Biological Indicator with the color-coded
configuration stick surrounding the 3M™ Attest™ Auto-reader incubation wells.
Do not remove or change placement of 3M™ Attest™ Biological Indicator once it is placed
into a well.
Do not incubate a 3M™ Attest™ Biological Indicator if, after processing and before BI
activation, it is observed to have a broken media ampoule. Retest the sterilizer with a new
biological indicator.
Do not remove the 3M™ Attest™ Biological Indicator from the incubation well until the (+) or
(‑) symbol on the LCD Display indicates the test is complete.
CAUTION: To reduce the risk associated with incorrect results.

To avoid the possibility of the 3M™ Attest™ Biological Indicator vial absorbing fluorescent
residue from a chemical indicator or tape, place the 3M™ Attest™ Biological Indicator vial so
it does not come in direct contact with chemical indicators or tape.

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3.1 Device Safety Compliance

The 3M™ Attest™ Auto-reader complies with the following standards as demonstrated by the
CB Scheme Certificate and test report issued by Underwriters Laboratories (UL):
 IEC 61010-1 (2001) Second Edition. Safety requirements for electrical equipment for
measurement, control, and laboratory use – Part 2-010: Particular requirements for
laboratory equipment for the heating of materials.
 IEC 61010-2-010 (2003) Second Edition. Safety requirements for electrical equipment for
measurement, control, and laboratory use – Part 2-010: Particular requirements for
laboratory equipment for the heating of materials.
The 3M™ Attest™ Auto-reader is listed as Laboratory equipment and carries the UL mark with
adjacent indicators “C” and “US” based on compliance to the standards UL 61010-1,
CAN/CSA C22.2 No. 61010-1, and CAN/CSA C22.2 No. 61010-2-010.
The 3M™ Attest™ Auto-reader complies with the CE mark related to the Low Voltage Directive
(LVD) 2006/95/EC as confirmed in the Declaration of Conformity.
The 3M™ Attest™ Auto-reader complies with the RoHS Directive, Directive 2011/65/EU of the
European Parliament and of the Council of 8 June 2011 on the restriction of the use of certain
hazardous substances in electrical and electronic equipment.
The 3M™ Attest™ Auto-reader complies with the WEEE Directive, Directive 2012/19/EU of the
European Parliament and of the Council of 04 July 2012 on waste electrical; and electronic
equipment (WEEE).

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3.2 EMC Compliance

The 3M™ Attest™ Auto-reader complies with the following EMC standards as confirmed in the
Certificate of Compliance generated by 3M:
 IEC 61326-1 Electrical equipment for measurement, control and laboratory use - EMC
requirements - Part 1: General requirements
 EMC requirements of the CE mark EMC Directive 2004/108/EC.
The 3M™ Attest™ Auto-reader complies with the Australian and New Zealand electrical safety
and electromagnetic compatibility requirements as confirmed in the Supplier’s Declaration of
Conformity that is linked to the Australian/New Zealand RCM (Regulatory Compliance Mark).
(Note: This equipment has been tested and found to comply with the limits for a Class A digital
device pursuant to Part 15, Subpart B, of the FCC Rules. These limits are designed to provide a
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, and can radiate radio frequency energy
and, if not installed and used in accordance with the instruction manual, may cause harmful
interference to radio communications. Operation of this equipment in a residential area is likely
to cause harmful interference in which case the user will be required to correct the interference
at their own expense.)
This Class A digital device meets all requirements of the Canadian Interference Causing
Equipment Regulations.

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3.3 Explanation of Product and Packaging Label Symbols
Attention - Refer to the instructions for use.
Waste Electrical and Electronic Equipment (WEEE) and EU Battery Directive

LI
UL Listed to US and Canadian Safety Standards.
Mark of Conformity to European Directives.
Direct current
Compliant to all applicable ACMA regulatory arrangements (RCM)
Manufacturer – This symbol is accompanied by name and address of the

manufacturer.
Catalogue – This symbol is accompanied by the catalogue number relevant to the

device bearing the symbol.
Authorized representative for the European Community – This symbol is

accompanied by the name and the address of the authorized representative in the
European Community.

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The 3M™ Attest™ Auto-reader 390G is not designed to incubate 3M™ Attest™
1264 EO Biological Indicators, which have a sterilant entry port in the center of
the top of the cap. The 3M™ Attest™ Auto-reader 390G is designed to incubate
and read only 3M™ Attest™ Rapid Readout Biological Indicators for EO, catalog
number 1294.
The 3M™ Attest™ Auto-reader 390 is not designed to incubate 3M™

Attest™ Biological Indicators 1261 or 1262, which have a sterilant entry
port in the center of the top of the cap. The 3M™ Attest™ Auto-reader 390 is
designed to incubate and read only 3M™ Attest™ Rapid Readout Biological
Indicators for Steam, catalog numbers 1291 and 1292. Other biological indicators
(BIs) are not compatible with these devices and cannot be used.

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4.0 WARRANTY AND SERVICE INFORMATION
Warranty
The 3M™ Attest™ Auto-reader has a one year limited product warranty. The US warranty,
remedy and limitations are described in the Price Quote form and in the Direct Price Pages. For
countries outside of the US, any warranty is established by the subsidiary with responsibility for
service of the 3M™ Attest™ Auto-reader.
Service Information
In the U.S.A., 3M™ Attest™ Auto-readers must be repaired at:
3M Health Care Service Center
Suite 200, Bldg. 502
3350 Granada Avenue North
Oakdale, MN 55128
1-800-292-6298
Fax: 1-800-770-8016
If you have questions, call our Customer Service number: 1-800-292-6298.
Outside of the U.S.A., contact your local 3M Subsidiary.

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5.0 INSTALLATION AND OPERATION
5.1 Initial Setup
Choose Location
Place the Auto-reader on a firm and level surface, away from sunlight and incandescent light.
Select and Install Correct Power Plug

The Auto-reader power supply comes with multiple blade configurations. Install the blade
configuration appropriate for your country into the power supply and plug into appropriate AC
grounded outlet.
Output
Plug
Power Supply Power Supply

Blade Configurations
Figure 5.1 - Auto-reader Power Supply with Blades

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5.2 Description of Front Panel
390/390G
Only
Figure 5.2 - 3M™ Attest™ Auto-reader Front Panel
Item numbers below refer to numbered items in the figure above.
Wells
1. Incubation Wells – The Auto-reader has ten incubation wells.
Buttons
2. Information / Up Arrow Button.
3. Temperature / Down Arrow Button.
4. Buzzer Alarm Off/Enter Button.
Information Display
5. LCD Display – Display is backlit.
6. LCD Display – Example of Error Message for well #6.
7. LCD Display – Example of Remaining Incubation Time for well #10.
8. LCD Display – Example of Negative ( - ) BI result for well #9.
9. LCD Display – Example of Positive ( + ) BI result for well #8.
10. LCD Display – Alarm Enabled Indicator.
Color Coded Configuration Indicator

11. Color-coded incubation well configuration sticker.
BI Crusher (Models 390 and 390G Only)

12. BI Crusher Well.

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5.3. Instructions for Use
To configure the unit, press the and buttons on the front of the Auto-reader
simultaneously for 3 seconds to enter configuration mode. Once in configuration mode, use the
Up and Down buttons to set the Auto-reader clock, buzzer and incubation well
configuration as desired. The button acts as an “Enter” key and moves the flashing cursor
to the next changeable setting.
Setting the Auto-reader Clock
The Auto-reader date and time are displayed in this format: YYYY-MM-DD HH:MM, with the
time displayed using a 24 hour clock. To change the year, if necessary, push the up or down
buttons to set desired year, then press to advance to the month, adjust as necessary and
press to advance to the day. Use the button to move to the time and set the hours and
minutes.
Setting the Audible Alarm Function

Move across the top row of text by pushing the button. The flashing “B” in Buzzer indicates
this is a changeable setting. It is recommended this be set at “Buzzer Enabled” to audibly alert
the operator to a positive BI but it may be set to “Buzzer Disabled” by using either the up or
down button.
(Note: If Buzzer Disabled is selected, and the changes accepted, the speaker icon ( ) will
disappear from the LCD panel and the alarm will be disabled for all positive Biological
Indicators, error codes, and caution code alarm warnings.)
(Note: The Auto-reader display backlight flashes whenever an audible alarm sounds. Disabling
the audible alarm does NOT disable the LCD display backlight flashing.)
Press to select the desired option and advance to the bottom row of text.

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Setting the Incubation Well Configuration
Models 390G and 490H

All incubation wells are fixed to run the same type of 3M™ Attest™ biological indicator.
Models 390 and 490

Incubation wells 1-5 and 6-10 can be configured to run the same or different types of 3M™
Attest™ biological indicators. The flashing “A” in Attest indicates the setting may be changed by
the user. There are three incubation well configurations.
LCD Panel Auto-reader

Result
Configuration Setting 390 390G 490 490H
Auto-reader wells 1 - 5
configured for 1291 BIs (blue
Attest 1291 Attest 1292 cap, 60-minute readout) and X
wells 6 - 10 configured for 1292
BIs (brown cap, 3-hour readout)
Auto-reader wells 1 – 10
Attest 1291 Attest 1291 configured for 1291 BIs (blue X
cap, 60-minute readout)
Attest 1292 Attest 1292 configured for 1292 BIs (brown X
cap, 3-hour readout)
Auto-readers wells 1 – 10
Attest 1294 Attest 1294 configured for 1294 BIs (green X
Auto-reader wells 1 - 5
configured for 1491 BIs (blue
Attest 1491 Attest 1492 cap, 30-minute readout) and X
wells 6 - 10 configured for 1492
BIs (brown cap, 1-hour readout)
Attest 1491 Attest 1491 configured for 1491 BIs (blue X
cap, 30-minute readout)
Attest 1492 Attest 1492 configured for 1492 BIs (brown X
Auto-readers wells 1 – 10
Attest 1295 Attest 1295 configured for 1295 BIs (pink X

5-4 78-8083-3058-9, Rev C
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Release Date:4/15/2015 8:15:12 PM
Use the Up Arrow or Down Arrow buttons to move between the three options for the Model 390 or
Model 490 Auto-reader until the desired configuration setting is displayed.
Press to select the desired option and advance to the Accept screen.
Note 1: If there are any incubating BIs which do not yet have a final result (+ or -) displayed,
the well configuration for the occupied wells will NOT change until the final result is displayed on
the LCD panel and/or the BI is removed from the reader.
Note 2: Ensure the color-coded incubation well configuration sticker surrounding the incubation
wells matches the programmed well configuration.
Accept Screen
Use the up or down buttons to toggle between Yes and No, press to accept changes (Yes
selected) or retain current settings (No selected) and exit the configuration mode. The LCD
panel will return to the standard display

78-8083-3058-9, Rev C 5-5
Status: Release
Release Date:4/15/2015 8:15:12 PM
6.0 PRINCIPLES OF OPERATION
6.1. Systems Overview - 3M™ Attest™ Auto-reader

The 3M™ Attest™ Auto-reader electronics hardware is shown in the block diagram below. An
Auto-reader consists of three printed circuit board assemblies, an LCD display with front panel
membrane switches, an incubation heater block and an external power supply to power the
instrument.
Main PCB Assy
Figure 6.1 - 3M™ Attest™ Auto-reader Electronics Hardware Block Diagram

6-1 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
Auto-reader electronics hardware functional details are as follows:
1. Printed Circuit Board (PCB) Assemblies
a. Main PCB Assembly – Provides the control functions for the LED PCB Assembly,
Sensor PCB Assembly, LCD display, and incubation heater block. It coordinates the
interactions between the PCB assemblies and their dependencies.
b. LED PCB Assembly - Contains ten ultra-violet LEDs, one for each sample well. The
LEDs serve as the excitation source for the BI.
c. Sensor PCB Assembly (also known as BI Detector Board) - Contains ten reflective
photo interrupters which are used to detect the presence of a BI. The approximate BI
cap position is detected for the Model 490 and Model 490H Auto-readers.
2. Front Panel Display Assembly
a. 40 Character x 2 line LCD Display - Shows the well number, incubation time
remaining, BI results status, and caution/error codes for each well. The date/time,
alarm status, incubation block temperature, and configuration information are user
accessible.
b. Three-button Membrane Switch Overlay
1) Alarm Mute Button - Enables the user to mute and unmute the audible alert tone.
2) Information/Up Arrow Button – Allows user to view time, date, and Auto-reader
configuration information
3) Temperature/Down Arrow Button - Allows user to view incubator block

temperature.
3. Resistive flexible heater – Heat source mounted to the BI incubator block
An Ethernet/RJ45 port allows communication with a personal computer. Auto-reader embedded

software allows display of BI status and results on a PC web browser.

78-8083-3058-9, Rev C 6-2
Status: Release
Release Date:4/15/2015 8:15:12 PM
6.2. Auto-reader Component Description
10
Main PCB Assy

4
1
9 Buzzer
2
3
7
5
8 6
11
Figure 6.2 - 3M™ Attest™ Auto-reader Main PCB Assembly Components Block Diagram
Item numbers below refer to the numbered items in the figure above.
Embedded Controllers
1. Main Microcontroller – The primary functions are to: a) Control the excitation source
driver circuit; b) Detect BI sample insertion/activation; c) Obtain emission readout
from the “Optics Microcontroller”; d) Store and retrieve data from the EEPROM; e)
Control the “Display Microcontroller”; f) Communicate with a host computer via an
Ethernet communication link; and g) Measure on-board temperature sensor.
2. Optics Microcontroller – The primary functions are to: a) Control the fluorescence
detection circuit; b) Perform emission measurements analog-to-digital (A/D)
conversion; c) Send fluorescence data to the “Main Microcontroller”; and d) Provide
incubation block temperature control.

6-3 78-8083-3058-9, Rev C
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Release Date:4/15/2015 8:15:12 PM
3. Display Microcontroller – The primary functions are to: a) Receive information from
the “Main Microcontroller”; b) Generate character sets; c) Display the information on
the LCD screen; and d) Capture information from the button interface.
Power
4. Power Supply Circuits – The Auto-reader receives 12 VDC regulated input power from
an external power supply and converts the input power into the DC levels required by
the Auto-reader circuitry.
Optics
5. Excitation Source Driver – The driver circuit is a 10-channel constant current driver.
Each channel is controlled individually where the output current on each channel is
set from the “Main Microcontroller.” The driver connects to an array of ultra-violet
LEDs on the LED PCB assembly
6. Photodiode Array/Integration Instrumentation Amplifiers – There is a 10 photodiode

array mounted on the Main PCB to acquire BI relative fluorescence. The
instrumentation amplifiers generate a voltage that is proportional to the relative BI
fluorescent response intensity. This voltage is converted to a digital value within the
“Optics Microcontroller.”
Heater Control
7. Heater Control – The heater control is a closed loop system which monitors the
incubation heater block temperature. The incubator block heater is turned on and off
to maintain the block temperature at a predetermined set point.
BI Insertion Detection Sensors
8. BI Detector Board – There are ten reflective photo-interrupters mounted on a “Sensor

PCB Assembly” that are used as proximity sensors to monitor BI insertion and
removal, one for each BI incubator well. The sensors enable the “Main
Microcontroller” to monitor BI insertion and removal.
Alert Tone
9. Buzzer – The “Main PCB Assembly” contains a sound element which generates an
audible alert tone to the user when: a) A positive (+) BI result occurs; and b) An error
condition has occurred.
Communications
10. Communications – Three types of serial communication are used to communicate

between microcontrollers, internal peripherals, and the device driver: a) RS232; b)
Inter-Integrated Circuit (I2C); and c) Serial Peripheral Interface (SPI). External
communication with a host computer utilizes an Ethernet connection.

78-8083-3058-9, Rev C 6-4
Status: Release
Release Date:4/15/2015 8:15:12 PM
User Interface
11. Display/Front Panel Buttons – A 40 Character x 2 line LCD provides: a) Status and
error code information feedback to the user for each BI inserted into an incubator/
reader well; and b) Configuration information. A front membrane panel with three
buttons—“Alert Mute,” “Temperature/Down,” and “Information/Up”—are used to: a)
Obtain status information; and b) Change the instrument configuration.
Fluorescence Detection
The figure below shows an Auto-reader optical path cross section and the related
electrical circuit functional blocks used to process the optical signals.
Figure 6.3 - Auto-reader Electro-Optics Apparatus
The excitation UV LED light transmits through the glass excitation filter onto the
biological indicator to excite the fluorophore. Emission light from the excited
fluorophore is transmitted through both the glass and gel emission filters and
absorbed by the photodiode sensor. The emission filters prevent non-emission light
from reaching the photodiode sensor.
Each of the 10 Auto-reader wells has an ultra-violet LED, a set of excitation/emission

filters, and a photodiode.

6-5 78-8083-3058-9, Rev C
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Release Date:4/15/2015 8:15:12 PM
7.0 MAINTENANCE
Care and Cleaning
CAUTION: Always unplug the Auto-reader and allow to cool before cleaning.
Do not spill liquid onto or into the instrument. Do not immerse the unit in liquid.
Clean external surfaces using only the instructions provided by the manufacturer.
The instrument must be returned to the manufacturer for repair.
Clean the Auto-reader exterior surface by wiping it with a damp cloth moistened with a mild
detergent. Do not attempt to clean interior components. If additional cleaning of the wells is
required, call for repair or replacement.

78-8083-3058-9, Rev C 7-1
Status: Release
Release Date:4/15/2015 8:15:12 PM
8.0 TROUBLESHOOTING GUIDE

The LCD display panel will indicate detected malfunctions by displaying a caution or error code
under the affected BI well(s). The audible alarm, if active, sounds and the LCD display backlight
flashes to alert the user to all caution (except C1) and error codes. The alarm and backlight can
be silenced and extinguished, respectively, by pressing the Buzzer Alarm OFF button . Note
that disabling the audible alarm does not disable the flashing LCD Display backlight.
In the event of an error code, unplug the Auto-reader and plug it back in to initiate the self-
diagnostic test. If error code re-occurs, begin diagnostics and repair procedures.
Caution Code Description

C1 .............................Unit Warm Up
C2 ........BI Removed from Well During Incubation
C3 ............................. Not Assigned
C4 .............................Ambient Light
Error Code Description

E1........................ Temperature Control
E2.............................. Not Assigned
E3.............................. Memory Data
E4.............................. Not Assigned
E5.............................. Not Assigned
E6.............................. Not Assigned
E7......................Communications Failure
E8........Excitation LED or Optics Detection Failure
E9.............................. Not Assigned
E10............................. Not Assigned
E11...................... BI Inserted Incorrectly
E12.....................Extended Power Failure
E13............................. Not Assigned
E14.............................Ambient Light

8-1 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
8.1. Caution Codes

C1: Unit Warm-Up Caution
Description: The incubation block is not at set point from start to 30 minutes.
Checked: When power is applied to unit (unit is plugged in).
Possible Causes:
1. Unit recently plugged in and not warmed to set temperature.
2. Loss of power caused block to cool down below set temperature.
Clearing: Caution code will clear when unit reaches temperature.
Notes:
1. Biological indicator readings are disabled until incubator block reaches set point
temperature.
Model Set Point Temperature

390 ..................... 60°C ± 2°C
390G .....................37ºC ± 2ºC
490 .................... 56.5°C ± 2°C
490H .................... 60°C ± 2°C
2. If unit does not reach set point temperature in 30 minutes, unit will display E1.
3. Temperature set point is fixed per BI type. The temperature set point cannot be
changed.
C2: BI Removed From Well Caution
Description: Biological Indicator has been removed before incubation is complete.
Checked: BI in well is constantly monitored during incubation.
Possible Cause: BI removed by operator before incubation was complete.
Clearing:
1. Replace BI in the same well from which it was removed within 10 seconds; or,
2. Do not replace BI (Non-result shown – Well record will indicate empty well).
Note: If BI is not replaced within 10 seconds, a non-result is shown in the record.

78-8083-3058-9, Rev C 8-2
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Release Date:4/15/2015 8:15:12 PM
C4: Ambient Light Caution
Description: Ambient light is interfering with reading of biological indicator; may occur
with or without a biological indicator in the well.
Checked: Monitored during BI insertion.
Possible Causes:
1. Auto-reader is positioned directly under incandescent light or in direct sunlight.
2. BI cap not closed tightly.
3. BI inserted incorrectly.
Clearing: Move Auto-reader to reduced ambient light conditions.

8-3 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
8.2. Error Codes

E1: Temperature Control Error
Description: The Auto-reader system is no longer able to control the temperature of the
incubator block.
Involved Components:
1. Thermister on Heater Block
2. Heater Blanket
Possible Causes/Solutions:
Cause Solution(s)
Unit does not meet Environmental Re-position unit in proper environment.
Operating requirements.
Heater Blanket has failed. Resistance of blanket should be
14.4 Ω ± 1.4 Ω. Replace heater block
assembly if specification not met.
Blanket is no longer adhering to well
block. Replace heater block assembly.
Blanket connector is not attached. Re-
attach connector.
Blanket connector is damaged and not
making contact. Replace heater block
assembly.
Thermister has failed. Resistance of thermister to be
10 kΩ ± 1 kΩ. Replace heater blanket if
specification not met.
Clearing: Unplug the unit and plug it back in to clear error.
Note: If E1 is displayed and power supply is disconnected from Auto-reader, Auto-reader

will try to reheat to set temperature.

78-8083-3058-9, Rev C 8-4
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Release Date:4/15/2015 8:15:12 PM
E3: Memory Error
Description: The Auto-reader is unable to save/read data from memory.
Involved Components: EPROM
Possible Cause/Solution:
Cause Solution
Bad Main Controller board. Replace Main Controller board.
Notes:
1. Can cycle power to attempt to recover from error.
2. Memory is constantly monitored during incubation.
E7: Communications Error
Description: The internal communications of the Auto-reader have failed.
Involved Components: PICS on the main controller board
Cause Solution
Pins shorted on programming connector Remove reason for shorted pins.
header.
Bad circuit trace on main controller board. Replace main controller board.
Faulty/damaged microcontroller Replace main controller board.

8-5 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
E8: Excitation LED or Optics Detection Error
Description: A UV LED or Optics detection circuit has failed for any of the BI wells.
1. UV LEDs
2. LED Circuit Board
Possible Causes / Solutions:

Cause Solution
Main Controller board to LED board Attach connector.
connector not connected.
UV LED Amplifier failed on Main Replace Main Controller board.
Controller board.
UV LED board failed. Replace UV LED board.
Notes:
1. The LEDs are checked every time the BI has a reading taken.
2. Run MTS service program to verify error.

78-8083-3058-9, Rev C 8-6
Status: Release
Release Date:4/15/2015 8:15:12 PM
E11: Biological Indicator Inserted Incorrectly Error
Description: The Biological Indicator was put into the well incorrectly.
Involved Components: Biological Indicator Detection System.
Cause Solution(s)
Glass ampoule was not crushed Remove BI from well and use crusher
wells (390/390G) on the top of the Auto-
reader or fully depress BI cap
(490/4990H) to crush ampoule. Return BI
to incubation well.
Cap was not fully pushed down Remove BI from well and firmly push cap
down. Cap must be pushed down
completely. Return BI to incubation well.
Growth media has not wet-out spore strip Remove BI from well and agitate BI to
ensure media saturated spore strip.
Return BI to incubation well.
Cloudy filter Run “Fluorescent Detection” in “Single
Test” mode in MTS program. Failure
result will be indicated.
Replace filter.
Bad LED board See E8 above.
Clearing: Correctly inserted BI will clear the error. If error persists, run “Fluorescent
Detection” in “Single Test” mode in MTS program to determine if there is a
filter issue.
Notes:
1. Checked on initial insertion of BI.
2. Incubation time remaining will not display and fluorescent readings are not taken.

8-7 78-8083-3058-9, Rev C
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Release Date:4/15/2015 8:15:12 PM
E12: Extended Power Failure Error
Description: Power to the Auto-reader was off for too long and incubator well
temperature has fallen to or below the follow temperature thresholds:
Model Temperature Threshold

390 .......................... 50°C
390G ..........................32ºC
490 .......................... 50°C
490H ......................... 50°C
Involved Components: Heater Blanket Thermister

Cause Solution
Disconnected power connector. Reconnect power supply.
Unit un-plugged from wall supply. Plug power cord back into wall supply.
Facility power failure. Correct facility power supply.
Thermister has failed. Resistance of thermister to be
10 kΩ ± 1 kΩ. Replace heater block
Heater blanket has failed. Resistance of heater blanket should be
14.4 Ω ± 1.4 Ω. Replace heater block
Clearing: Reconnect power or repair failure.
Notes:
1. Temperature of heater blanket is checked when power is returned.
2. BI result is NOT valid.
3. Only well(s) containing BI(s) will indicate E12.
4. Remove all BIs if error occurs.

78-8083-3058-9, Rev C 8-8
Status: Release
Release Date:4/15/2015 8:15:12 PM
E14: Ambient Light Error
Description: Too much ambient light has interfered with the Biological Indicator reading.
Involved Components: BI Detection board
Cause Solution
The Auto-reader is placed directly under Move Auto-reader out of direct fluorescent
fluorescent light. light.
The Auto-reader is placed in direct Move Auto-reader out of direct sunlight.
sunlight.
BI moved out of well during another BI Reseat BI.
insertion.
Clearing: Move Auto-reader out of direct light.
Notes:
1. The fluorescent result will not display in affected well.
2. User to incubate BI and rely on visual pH color change for result.

8-9 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
8.3. Other Service Conditions

8.3.1. Unwanted Alarm Sounds When Positive Biological Indicator Detected.
Description: When a BI is positive, the alarm buzzer is activated.
Involved Component: System buzzer
Cause Solution
Customer did not want alarm to sound and Disable buzzer. Refer to the “Setting the
forgot to silence buzzer. Audible Alarm Function” in the “Instructions
for Use” section in this manual.
Touch Pad failed. Replace Upper Housing with Overlay
Assembly.
Touch Pad Cable not connected. Reconnect cable.
Touch Pad Cable Failed. Replace Upper Housing with Overlay
Assembly.
Clearing: Not Applicable–Customer preferred action.
8.3.2. LCD Panel Does Not Display Properly
Description: There is no display when power is applied to the Auto-reader.
1. Auto-reader LCD (Liquid Crystal Display)
2. LCD ribbon cable
Cause Solution(s)
Display cable not connected. Connect LCD ribbon cable.
LCD Assembly has failed. Replace LCD Assembly.
Power supply not connected or failed. Connect power supply.
Replace power supply.
Notes:
1. Can cycle power to attempt to correct problem.
2. If LCD is still blank perform diagnostics as listed above.

78-8083-3058-9, Rev C 8-10
Status: Release
Release Date:4/15/2015 8:15:12 PM
8.3.3. Unable to Communicate with Auto-reader, Static IP Address Set
Description: Unable to communicate with Auto-reader because the unit is configured

with a static IP address.
Involved Component: Main Controller Board
Cause Solution
Unit configured to use a static IP address.
Depress the Auto-reader Information
and Temperature buttons
simultaneously for 10 seconds to enable
DHCP.
Corrupted or incorrect webpage. Use MTS to reload the webpage.

8-11 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
9.0 DISSASSEMBLY / ASSEMBLY INSTRUCTIONS

9.1. Top Cover Removal
9.1.1. Disconnect all external cables from unit under repair.
9.1.2 Carefully place the unit top down on a level surface that will protect the unit from being
scratched or damaged.
9.1.3. Remove the six M3 hex screws from the “Lower Housing”.
Screws
Figure 9.1 - Auto-reader Bottom View
9.1.4. Turn the unit right side up while holding the “Upper Housing” to the “Lower Housing”
so the unit is facing forward.

78-8083-3058-9, Rev C 9-1
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Release Date:4/15/2015 8:15:12 PM
9.1.5. Remove the “Upper Housing” from the “Lower Housing, hinging the “Upper Housing”
to the left.
Note: Take care not to overstrain the button overlay cable and the ribbon cable
assemblies. The red stripe on the ribbon cable is toward the BI Block when attached to
the Main Board connector and toward the upper housing BI opening on the LCD Board.
Ribbon Cable
Red Stripe Toward

BI Block
Red Stripe Toward

Button Overlay
Upper Housing Opening
Cable
Figure 9.2 - Auto-reader Upper and Lower Housing Opened

9.1.6. Disconnect the button overlay cable and the ribbon cable from the “BI Block/PCB
Assembly”.

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Release Date:4/15/2015 8:15:12 PM
9.2. LCD Display Assembly Removal
9.2.1. Carefully place the “Upper Housing” top down on a level surface that will protect the
unit from being scratched or damaged.
9.2.2. Remove the four M3 torx screws holding the “LCD Display Assembly” to the “Upper
Housing”.
Note: The red stripe on the ribbon cable is oriented toward the “LCD Display Assembly”
header “CN1”, pins 15 and 16.
Screws Red stripe toward

CN1 pins 15 & 16
Figure 9.3 - “LCD Display Assembly” Mounted In “Upper Housing”

9.2.3. Remove the “LCD Display Assembly”.
Note: The “LCD Display Assembly” consists of a “Display Board” and an “LCD Display”.

78-8083-3058-9, Rev C 9-3
Status: Release
Release Date:4/15/2015 8:15:12 PM
9.3. Button Overlay Assembly
The “Button Overlay Assembly” is not to be removed from the “Upper Housing”. If the
overlay assembly buttons fail, the “Upper Housing” and the “Button Overlay Assembly”
should both be replaced with the “Upper Housing With Overlay” spare part.
Upper Housing
Button Overlay
Assembly
Figure 9.4 - “Upper Housing With Button Overlay” Spare Part”
9.4. BI Block/PCB Assembly Removal
9.4.1. Remove the six M3 torx screws holding the “BI Block/PCB Assembly” to the “Lower
Housing”.
Screws
Figure 9.5 - BI Block/PCB Assembly

9.4.2. Remove the “BI Block/PCB Assembly” from the “Lower Housing”.

9-4 78-8083-3058-9, Rev C
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9.5. Sensor PCB Assembly (BI Detection Board) Removal
9.5.1. Remove the 3-inch “Parlex Flat Cable” from both the “Sensor PCB Assembly” and the
“Main PCB Assembly”. Note the cable orientation for later re-assembly.
9.5.2. Remove the four M3 torx screws holding the “Sensor PCB Assembly” to the
“Incubator Block Housing”.
Sensor PCB
Assembly
3” Parlex Screws
Flat Cable
Main PCB
Assembly
Figure 9.6 - Sensor PCB Assembly Removal
9.5.3. Remove the “Sensor PCB Assembly” from the “Incubator Block Assembly”.
9.6. LED PCB Assembly Removal
9.6.1. Remove the 2-inch “Parlex Flat Cable” from both the “LED PCB Assembly” and the
“Main PCB Assembly”. Note the cable orientation for later re-assembly.
9.6.2. Remove the four M3 torx screws holding the “LED PCB Assembly” to the “Optics
Alignment Block”.
2” Parlex
Flat Cable
LED PCB
Assembly
Screws
Main PCB
Assembly
Figure 9.7 - LED PCB Assembly Removal

6.3. Remove the “LED PCB Assembly” from the “Optics Alignment Block”.

78-8083-3058-9, Rev C 9-5
Status: Release
Release Date:4/15/2015 8:15:12 PM
9.7. BI Block Removal
9.7.1. Remove the 2-inch “Parlex Flat Cable” from the “Main PCB Assembly”. Note the
cable orientation for later re-assembly.
9.7.2. Remove the 3-inch “Parlex Flat Cable” from the “Main PCB Assembly”. Note the
cable orientation for later re-assembly.
9.7.3. Remove the “Heater Flex Assembly” cable from the “Main PCB Assembly”.
Screws
Heater Flex 2” Parlex

Assembly Flat Cable
Cable
3” Parlex
Flat Cable
Main PCB
Assembly
Figure 9.8 - BI Block/Main PCB Assembly

9.7.4. Remove the two M3 torx screws holding the “Optics Alignment Block” to the “BI
Block”.
9.7.5. Carefully turn the assembly over so that the “Main PCB Assembly” is facing upward
and the “”Incubator Block” is rearward.
Screws
Main PCB
Assembly
Figure 9.9 - BI Block/Main PCB Assembly (Bottom View)

9.7.6. Loosen by ½ turn the four M3 torx screws holding the “Incubator Block Housing” to
the “Optics Alignment Block”.

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9.7.7. Remove the “Heater Block Assembly” from the “Optics Alignment Block/Main PCB
Assembly”.
Note: The upper and lower optical filters remain captured in the Optic Alignment Block”.
Heater Block
Assembly
Optics Alignment
Block Assembly
Main PCB
Assembly
Figure 9.10 - Heater Block Assembly Removal

9.7.8. Remove the six M2 hex screws holding the “Incubator Block Assembly” to the
“Incubator Block Housing”.
Screws
Figure 9.11 - Heater Block Assembly
9.7.9. Remove the “Incubator Block Assembly” from the “Incubator Block Housing”.
Incubator Block
Assembly
Incubator
Block Housing
Figure 9.12 - Incubator Block Assembly Removal
Note: If the “Heater Flex Assembly”, Incubator Block Insulator” or thermistor has failed,
the complete “Incubator Block Assembly” must be replaced.

78-8083-3058-9, Rev C 9-7
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Release Date:4/15/2015 8:15:12 PM
9.8. Main PCB Assembly Removal
9.8.1. Orient the “Main PCB Assembly/Optics Alignment Block/Optics Alignment Block
Cover” assembly such that the “Main PCB Assembly” bottom is facing upward.
Screws
Main PCB
Assembly
Figure 9.13 - Main PCB Assembly/Optics Alignment Block Assembly
9.8.2. Remove the four M3 torx screws holding the “Main PCB Assembly” to the “Optics
Alignment Block”.
9.8.3. Lift the “Main PCB Assembly” upward and off the “Optics Alignment Block” carefully to
prevent the glass and gel emission filters from falling out of the “Optics Alignment
Block”.
Note: During reassembly, ensure the ”Main PCB Assembly” slots are aligned with and
Tab Tab
Optics Alignment
Block Gel and Emission
Slot Slot Filters (x10)
Main PCB
Assembly
seated in the “Optics Alignment Block” tabs before inserting the M3 torx screws.
Figure 9.14 - Main PCB Assembly Removal

9-8 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
9.9. Glass and/or Gel Emission Filter Replacement
Note: Non-powder rubber or cotton gloves must be worn when handling the filters. Skin oils
and other contaminants must be kept off the filter surfaces. Examine replacement filters for
cleanliness. Use low volume, clean pressurized air to clean debris off filters surfaces. DO
NOT wipe surfaces with anything.
9.9.1. Perform the “Main PCB Assembly Removal” procedure to expose the glass emission
filters and gel emission filters.
9.9.2. Carefully and simultaneously remove both the “Gel Emission FIlter” and “Glass
Emission Filter” from the failed well.
Optics Alignment Block
LED PCB
Assembly
Glass Emission
Filter (Light Blue)
Gel Filter
(Light Blue)
Figure 9.15 - Gel and Emission Filter Removal
9.9.3. Insert the replacement filters with the “Gel Emission Filter” oriented toward the “Main
PCB Assembly and the “Glass Emission Filter” oriented toward the “Incubator Block
Housing”.

78-8083-3058-9, Rev C 9-9
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Release Date:4/15/2015 8:15:12 PM
9.10. Glass Excitation Filter Replacement
Note: Non-powder rubber or cotton gloves must be worn when handling the filters. Skin oils
and other contaminants must be kept off the filter surfaces. Examine replacement filters for
cleanliness. Use low volume, clean pressurized air to clean debris off filters surfaces. DO
NOT wipe surfaces with anything.
9.10.1. Remove the “LCD PCB Assembly” procedures, “Sensor PCB Assembly (BI
Detection Board)” and “Heater Block Assembly”.
9.10.2. Remove the four M3 torx screws holding the “Optics Alignment Block Cover” to the
“Optics Alignment Block”.
Note: Keep the “Optics Alignment Block” upright so the filters stay in place.
Optics Alignment
Block Cover
Optics Alignment
Block
Screws
Main PCB
Assembly
Figure 9.16 - Optics Alignment Block Cover Removal

9-10 78-8083-3058-9, Rev C
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9.10.3. Remove the failed filter
Note: LCD PCB Assembly shown installed for

clarity to show LED location relative to filter.
Main PCB
Assembly
Glass
Excitation Filter
(Black)
Optics
Alignment
Block
Figure 9.17 - Glass Excitation Filter Removal

9.10.4. Replace the failed filter and ensure there is a small gap between the UV LED and
the glass filter.
Note: When reassembling the “Optics Alignment Block Cover”, ensure there is no
glass straining/cracking heard or observed.

78-8083-3058-9, Rev C 9-11
Status: Release
Release Date:4/15/2015 8:15:12 PM
10. MANUFACTURING TEST SYSTEM (MTS) SETUP AND USE
10.1 Equipment Required

Auto-reader
Description 3M Part Number
390 390G 490 490H
Windows 7™ 32-bit or 64 bit SP1 or
greater PC with Manufacturing Test Locally supplied X X X X
System (MTS) Version 4.0 installed
Ethernet Cable – Cat 5, Cat 5e or Cat 6
Locally supplied X X X X
(Qty = 2)
Wired Router: TP-Link TL R860 or equiv.
Locally supplied X X X X
with DHCP enabled
Digital Multi-meter Locally supplied X X X X
Digital Thermometer, Fluke 51-II Locally supplied X X X X
Thermocouple Probe 78-0005-0452-8 X X X X
390 Calibration Standards 78-8083-3123-1 X X
1292 BI (with caps closed) 70-2006-2052-7 X
1294 BI (with caps closed) 70-2007-0460-2 X
490 Calibration Standards 78-0005-0404-9 X X
1492V BI (with caps open) 70-2007-6356-6 X X
1492V BI (with caps closed) 70-2007-6356-6 X X

10-1 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2. MTS SOFTWARE
10.2.1. Intended Use
The 3M MTS Software is a desktop suite of tools that are packaged together to perform
troubleshooting, calibration and verification along with setting basic device parameters for the
3M Attest™ Auto-readers. It is used by 3M Manufacturing and Service organizations to perform
calibrations and verify Auto-reader functionality. The data output from the “Verify” test
sequence is used by service personnel to complete a “Calibration Certificate” for the 3M
Attest™ Auto-readers.
Figure 10.1 - MTS Main Screen
IMPORTANT: At the completion of the 3M Attest™ Auto-reader repair, return the unit in
the same condition as received or as the customer has requested.

78-8083-3058-9, Rev C 10-2
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.2. MTS Software Description
The MTS test suite is comprised of software tools to: a) Set file path preferences; b) Manage
fluorescent standards; c) Upgrade the Auto-reader device firmware and webpage binary
images; d) Select test function modes to perform pre-preprogrammed test sequences. Each of
these components are detailed below.
File Path Preferences
Fluorescent Standards
Management
Firmware/Webpage Upgrades
Function Modes
Figure 10.2 - MTS Main Screen – Software Tools
10.2.2.1. File Path Preferences – Enables the user to define the file path locations for test data,
serial number generation and webpages.
10.2.2.2. Fluorescent Standards Management – Enables the user to enter standards

identification and fluorescent standards data. An internal alert will notify the user when
the standards have expired and need to be verified.
10.2.2.3. Firmware and Webpage Upgrades – A tool to upgrade the Auto-reader device
firmware and webpage binary images. The firmware and webpage software is
validated against its original MD5 hash code, just prior to upload, to ensure they have
not been corrupted.
(Note: The “Firmware Tool” And “Upload Webpage Tool” remain grayed out until an
Auto-reader is “connected”.)

10-3 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.2.4. Function Mode – Enables the user to select one of four function modes:
a) Manufacturing; b) Verify; c) Single Test; and, d) Serial Number.
A validated XML data file for the “Manufacturing” and “Verify” modes is created and saved in
the “c:\MTS\Tests” folder after the test is complete. The XML data file name convention is
as follows:
Attest_<S/N>_<Test date>_<Test time>_<Test type>_<Test result>.xml, where

<S/N> = Serial Number;
<Test date> = mm_dd_yyyy format, where mm = month, dd = day, yyyy = year;
<Test time> = hh_mm_ss format, where hh = hour, mm = minute, ss = second;
<Test type> = “VER” for Verify tests, “MFG” for Manufacturing tests;
<Test result> = “Pass” or “Fail”
If a “Calibration Certificate” for the 3M Attest™ Auto-reader is required, the data file for a
“Verify” test sequence that passed should be used as the data source to complete the
certificate (e.g. Attest_303033_10_09_2014_15_24_17_VER_Pass.xml).
NOTE: XML data files contain 3M CONFIDENTIAL information and shall not be given
to the customer or distributed in any manner.
10.2.2.4.1. Manufacturing – A test suite wizard that performs all “Calibration” and
“Verification” tests except for setting device time, serial number, and MAC
Address. All BI records are erased upon successful completion. In general, this
test should only be used by manufacturing.
“Calibration” Tests “Verification” Tests

1. Real Time Clock 1. Button Check
2. Battery Voltage 2. Alarm Check
3. BID Calibration 3. Firmware Check
4. VEM Calibration 4. Temperature Check
5. Heater Block Calibration 5. BI Insertion
6. Fluorescent Detection
10.2.2.4.2. Verify – A test suite wizard that performs all “Verification” tests.
10.2.2.4.3. Single Test – A user selectable test list that allows users to pick one or more
individual “Calibration” and “Verification” tests to troubleshoot a problem.
Selected tests are performed in sequence. MTS directs control back to the Main
Screen at the completion of the selected tests.
10.2.2.4.4. Serial Number – Sets the device time based upon PC time, assigns a unique
serial number, and assigns a MAC Address. The “manual mode” (versus the
“automatic mode”) should be used by service organizations. Only use this
function after the “Main Controller Board” has been replaced so that the original
serial number is assigned to the Auto-reader.

78-8083-3058-9, Rev C 10-4
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.3. MTS Software Installation
10.2.3.1. Open a web browser to the HCSC website http://www.3m.com/hcsc.
10.2.3.2. Select the “Attest Autoreaders – Incubators > Incubator Models” product group.
Product
Group
Figure 10.3 - HCSC Website Screenshot
10.2.3. Select the following in the “Incubator Models” drop-down boxes, then “View Results”:
Select Model: Model 390/390G/490/490H
Select Document Type: Bulletin
Model /
Document Type
Bulletin_A08
Figure 10.4 - HCSC Website, Incubator Models Screenshot
10.2.3.4. Locate and download the most current MTS software/upgrade equipment bulletin.
(e.g. Bulletin_A08: Equipment Bulletin A08 – MTS 4.0.15 Software Upgrade)
10.2.3.5. Follow the equipment bulletin instructions to install and/or upgrade the MTS software.

10-5 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.4. Auto-reader Setup for Testing
10.2.4.1. Place the Auto-reader on a firm level surface away from sunlight and incandescent
light.
10.2.4.2. Connect the Auto-reader to the router and the router to the personal computer as
shown below.
Ethernet Ethernet
Cable Cable
Wired Router
Auto-reader
PC / MTS
Power PC Auto-reader
Note: Power supplies not
Wired Router - Rear View shown for clarity.
Figure 10.5 - MTS Test Set-up
10.2.4.3. Install the Auto-reader power supply blade configuration appropriate for the country
into the power supply.
Output
Plug
Power Supply Power Supply

Blade Configurations
Figure 10.6 - Auto-reader Power Supply with Blades
(Note: The 3M™ Attest™ Auto-reader power supply is supplied with multiple blade
configurations that are country specific.)
10.2.4.4. Insert the power supply into an appropriate AC grounded outlet.

78-8083-3058-9, Rev C 10-6
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.4.5. Insert the power supply output plug into the Auto-reader.
10.2.4.6. Verify the Auto-reader “Pushbutton” and DHCP configurations are configured for
servicing.
10.2.4.6.1. Depress the “Information/Up” button twice to display the “IP Address:”
Figure 10.7 - Auto-reader Display – IP Address

10.2.4.6.2. Record the “IP Address” (e.g. 192.168.1.100).
10.2.4.6.3. Open a web browser on the PC connected to the Auto-reader.
10.2.4.6.4. Enter http://<IP Address>/protect/config.htm in the web browser address bar,

where <IP Address> is the Auto-reader IP Address, to access the Auto-reader
“Configuration” screen.
10.2.4.6.5. Enter the username and password (case-sensitive) information in the Windows
Security window when prompted.
Username = manager
Password = Attest
Figure 10.8 - Windows Security Prompt

10-7 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.4.6.6. Verify the “Pushbutton Configuration” is enabled and “DHCP” checkbox is

selected.
Pushbutton “Enable”
Selected
DHCP “checked”
“Save Config” button
Figure 10.9 - Auto-reader Configuration Screen

10.2.4.6.7. Click the “Save Config” button.
10.2.4.6.8. Close the web browser session.
10.2.4.7. Depress and hold down the Auto-reader “Buzzer Alarm Off/Enter” button and the
“Temperature/Down Arrow” button at the same time for at least three seconds
until the Auto-reader display shows “Auto-reader <====> PC” to unlock the Auto-
reader.
Figure 10.10 - Auto-reader Display – Auto-reader/PC Unlocked
The Auto-reader is now set up for communication with the MTS program for up to 24 hours or
until the unit is power cycled. If the unit is power cycled and MTS communication needs to be
restored, repeat this step.

78-8083-3058-9, Rev C 10-8
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.5. MTS Login and Connection
10.2.5.1. Double-click the MTS application program icon to open the MTS application.
10.2.5.2. Enter your Windows username and password when prompted at the MTS Login
screen, then click “Login.”
Windows Username
Windows Password
Click
Figure 10.11 - MTS Login Screen
(Note: If login fails, right click on the MTS application program icon, then select
“Properties” > “Security” tab. Locate your username in “()” in the “Group or user names:”
section. Use the username enclosed in the “()” as the MTS Login username.)
Select
Username
Figure 10.12 - MTS Properties Screen

10-9 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.2.5.3. Click the “Find Reader” button to find available networked Auto-readers and then
select the Auto-reader to service from the drop-down box.
Click
Select from
drop-down
list
Figure 10.13 - MTS Main Screen – Find Reader
10.2.5.4. Select the “Autoreader Type.”
Select
Autoreader
Type
Figure 10.14 - MTS Main Screen – Auto-reader Type
10.2.5.5. The Auto-reader is now connected and available to perform MTS service functions.

78-8083-3058-9, Rev C 10-10
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.3. File Path Preferences

MTS stores test result data, serial number generation/MTS configuration data xml files, and
webpage update files in specific folders. The folder locations are modified from the MTS
“Preferences” screen. A connected Auto-reader is not required to access “Preferences.”
10.3.1. Select “Preferences” from the MTS Main Screen.
Select
Figure 10.15 - MTS Main Screen – Preferences
10.3.2. Select the “Browse” buttons to navigate and change the folder to be modified.
Test result file location
Serial number generator and MTS

application configuration location
(“xml” files)
Webpage file locations
Figure 10.16 - MTS – Preferences Window

10-11 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.4. Fluorescent Calibration Standards Management

Auto-reader well integration times are set so that Auto-reader wells detect the “Calibration
Standards” fluorescent VEM readings. The calibration standards VEM data is manually entered
into MTS prior to initial use. Thereafter, MTS alerts the user when the standards have expired
and need to be verified. MTS login is not required to access “Add or Modify Standards.”
10.4.1. Standards Maintenance and Handling
390/390G Calibration Set 490 Calibration Set
Used with Used with

390 and 390G 490 and 490H
Auto-readers Auto-readers
(78-8083-3123-1) (78-0005-0404-9)
Figure 10.17 - Calibration Standard Sets
10.4.1.1. Fluorescent standards have unique assigned VEM readings for each calibration
standard that is printed on the label affixed to the standard. Standards should be
stored between 0°C and 29°C and not subjected to direct sunlight while not in use.
10.4.1.2. Fluorescent standards should be visually inspected prior to every use to ensure
that no dust or debris has collected in the mask area. If dust or debris has
collected, clean the affected areas by gently blowing the assemblies with clean/oil-
free compressed air. After cleaning, inspect mask and fluorescent material for
tightness and damage. DO NOT USE ISOPROPYL ALCOHOL ON MASK AREA.
10.4.1.3. Fluorescent standards should be gently shaken, prior to use, to ensure that no
rattling can be heard. A rattle indicates that the Mask/Fluorescent
Material/Retainer clip has come loose. In the event that a rattle is audible,
discontinue use of fluorescent standard.
10.4.1.4. It is critical to fully insert the calibration standards into the Auto-reader wells under
test. Failure to do so could result in incorrect calibration and failure during the
“VEM Sensitivity Verification” step.
10.4.1.5. A three minute enforced wait time occurs after inserting the fluorescent standards
into the Auto-reader wells under test prior to calibration to allow time for the
incubator block to recover to the block temperature set point.

78-8083-3058-9, Rev C 10-12
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.4.2. MTS Calibration Standards Data Input
10.4.2.1. Select “Add Or Modify Standards” from the MTS Main Screen.
Select
Figure 10.18 - MTS Main Screen – Add Or Modify Standards
10.4.2.2. Enter “Attest” for the password in the “Change Standards Password” screen, then
click on the “OK” button.
Enter “Attest”
Click
Figure 10.19 - MTS “Change Standards Password” Window

10-13 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.4.2.3. Locate the standards data from the “Calibration Standard Set” labels.
3
4 3 2 4 1 2
1. Standard set/position(s) 1. Standard S/N
2. 390 VEM value(s) 2. VEM value
(390G values listed below 390 values) 3. Calibration date
3. Calibration date 4. Calibration due date
4. Calibration due date
Figure 10.20 - 390/390G and 490 Calibration Set Data Label Detail
10.4.2.4. Enter data from the “Calibration Standard Set” label into the “Add New Standard”
window.
(Note: The 390/390G calibration set requires data entry for each standard in
positions C1-C5; the 490 calibration set requires data entry for each
ampoule standard.)
10.4.2.4.1. Select the ”Auto-reader Type.”

10.4.2.4.2. Enter the information from the standard set label into the “Standard
Identifier,” “Target VEM,” “Standard Calibration Date,” and “Standard
Calibration Expires” text boxes.
Select Auto-reader Type

Enter Standard Set “Set #/position” (390/390G) or
“S/N” (490)
Enter “VEM” value
Enter “calibration date”
Enter “calibration due date”
Click “Save” after data above selected/entered
Figure 10.21 - MTS “Add New Standard” Window

78-8083-3058-9, Rev C 10-14
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.5. Firmware and Webpage Upgrades

10.5.1. Firmware and webpage upgrades are performed with the “Firmware Tool.” If only the
webpage upgrade is required, refer to the “Webpage Upgrade” section. An Auto-reader
must be connected and unlocked in order to use this tool.
10.5.1.1. Select “Firmware Tool.”
Select
Figure 10.22 - MTS Main Screen – Firmware Tool
10.5.1.2. Navigate to and highlight the firmware “hex” file to program, then click on the
“Open” button.
“hex” file to program
Click
Figure 10.23 - Firmware Tool Screen – Upgrade File Selection Window
10.5.1.3. Enter the “MD5” checksum confirmation number, then click on the “OK” button.
“MD5” checksum
Click
Figure 10.24 - Firmware Tool Screen – “MD5 Checksum Confirmation” Window

10-15 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.5.1.4. Verify the “hex” file and “MD5” checksum are correct, then click the “Update”
button.
“MD5” checksum
Click
Figure 10.25 - Firmware Tool – “Device Firmware Update” Window
During the firmware upgrade, a command window screen will display while running the
program “tftp.exe.”
WARNING: DO NOT CLOSE THE COMMAND WINDOW SCREEN WHILE THE

“tftp.exe” PROGRAM IS RUNNING OR THE FIRMWARE UPLOAD PROCESS MAY BE
CORRUPTED. IF THE UPLOAD FILE IS CORRUPTED, THE AUTO-READER MAY
HAVE TO BE PROGRAMMED WITH A PROGRAMMER MODULE. WHEN THE
“tftp.exe” PROCESS IS COMPLETE, THE WINDOW WILL CLOSE AUTOMATICALLY.
Do NOT click the
“Close” icon
Figure 10.26 - Firmware Tool – Command Screen Running “”tftp.exe”
If the following window appears, click “OK” and refer to the “Firmware Recovery” section.
Click
Figure 10.27 - Firmware Tool – MTS FW Update Failed Window

78-8083-3058-9, Rev C 10-16
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.5.1.5. Click on the “OK” button when the “MTS Firmware Update Successful” window
displays.
Click
Figure 10.28 - Firmware Tool – “MTS Firmware Update Successful” Window
The Auto-reader will reboot and then lock out MTS functions. MTS will display a “User
Action Required” window.
10.5.1.6. Unlock the Auto-reader - Depress and hold down the Auto-reader “Buzzer Alarm
Off/Enter” button and the “Temperature/Down Arrow” button at the same time for at
least three seconds until the Auto-reader display shows
“Auto-reader <====> PC.”
10.5.1.7. Click on the “OK” button in the “MTS Firmware Update Successful” window.
Click
Figure 10.29 - Firmware Tool – “User Action Required” Window
10.5.1.8. Navigate to and highlight the web page “bin” file to program, then click on the
“Open” button.
“bin” file to program
Click

10-17 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.5.1.9. Enter the “MD5” checksum confirmation number, then click the “OK” button.
“MD5” checksum
Click
10.5.1.10. Verify the “bin” file and “MD5” checksum are correct, then click the “Update”
button.
“MD5” checksum
Click
Figure 10.32 - Firmware Tool – “Device Webpage Update” Window
During the webpage upload, a “Loading <Auto-reader Model #> Web Pages” window will
display the text “Uploading….”
10.5.1.11. Click on the “Close” button when the “Loading <Auto-reader Model #> Web
Pages” window displays “Web Pages Uploaded.”
Click
Figure 10.33 - Firmware Tool – “Loading 390 Web Pages” Window

78-8083-3058-9, Rev C 10-18
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.5.1.12. Click the “OK” button when the “MTS Webpage Upload” window displays.
Click
Figure 10.34 - Firmware Tool – “MTS Webpage Upload” Window

10-19 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.6. Webpage Upgrades

10.6.1. Webpage upgrades are performed with the “Upload Webpage Tool.” If both firmware
and webpage upgrades are required, refer to the “Firmware and Webpage Upgrade”
section. An Auto-reader must be connected and unlocked in order to use this tool.
10.6.1.1. Select “Upload Webpage Tool.”
Select
Figure 10.35 - MTS Main Screen – Upload Webpage Tool
10.6.1.2. Navigate to and highlight the web page “bin” file to program, then click on the
“Open” button.
Click
“MD5” checksum
Click

78-8083-3058-9, Rev C 10-20
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.6.1.4. Verify the “bin” file and “MD5” checksum are correct, then click the “Update”
button.
“MD5” checksum
Click
Figure 10.38 - Firmware Tool – “Device Webpage Update” Window
During the webpage upload, a “Loading <Auto-reader Model #> Web Pages” window will
display the text “Uploading….”
10.6.1.5. Click the “Close” button when the “Loading <Auto-reader Model #> Web Pages”
window displays “Web Pages Uploaded.”
Click
Figure 10.39 - Firmware Tool – “Loading 390 Web Pages” Window
10.6.1.6. Click on the “OK” button when the “MTS Webpage Upload” window displays.
Click
Figure 10.40 - Firmware Tool – “MTS Webpage Upload” Window

10-21 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.7. Firmware Recovery

10.7.1. The MTS “FW Recovery” tool is used to resume the firmware update process after a
firmware upgrade failure incident while using the MTS “Firmware Tool.” If the “FW
Recovery” upgrade described in this section fails, the Auto-reader must be updated with
a standalone programmer.
This section assumes a MTS “Firmware Tool” firmware update failure resulting in the
following: a) Auto-reader LCD displays the message “Bootloading. . .”; b) MTS displays a
“MTS FW Update Failed!!!” window; and c) Auto-reader buttons are inactive.
Figure 10.41 - Auto-reader “Bootloading…” Message
10.7.1.1. Click the “MTS FW Update Failed!!!” window “OK” button.
Click
Figure 10.42 - “MTS FW Update Failed!!!” Screen
10.7.1.2. Select “FW Recovery” from the MTS Main Screen.
Select
Figure 10.43 - MTS Main Screen – FW Recovery

78-8083-3058-9, Rev C 10-22
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.7.1.3. Navigate to and highlight the firmware “hex” file to program, then click on the
“Open” button.
Click
Figure 10.44 - FW Recovery Screen – Recovery Upgrade File Selection Window
“MD5” checksum
Click
Figure 10.45 - FW Recovery Screen – “MD5 Checksum Confirmation” Window
10.7.1.5. Verify the “hex” file and “MD5” checksum are correct, then click the “Update”
button.
“MD5” checksum
Click
Figure 10.46 - FW Recovery Screen – “Device Firmware Update” Window

10-23 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
During the firmware upgrade recovery, a command window screen will display while
running the program “tftp.exe”.
WARNING: DO NOT CLOSE THE COMMAND WINDOW SCREEN WHILE THE

“tftp.exe” PROGRAM IS RUNNING OR THE FIRMWARE UPLOAD PROCESS MAY BE
CORRUPTED. IF THE UPLOAD FILE IS CORRUPTED, THE AUTO-READER MAY
HAVE TO BE PROGRAMMED WITH A PROGRAMMER MODULE. WHEN THE
“tftp.exe” PROCESS IS COMPLETE, THE WINDOW WILL CLOSE AUTOMATICALLY.
Do NOT click the
“Close” icon
Figure 10.47 - Firmware Tool – Command Screen Running “”tftp.exe”
10.7.1.6. Click the “OK” button when the “MTS Firmware Update Successful” window
displays.
Click
Figure 10.48 - FW Recovery – “MTS Important Message!!!”

The firmware upgrade is now complete. If a webpage upgrade is required, refer to the
“Webpage Upgrades” section.

78-8083-3058-9, Rev C 10-24
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8. Test Descriptions and Procedures

MTS enables the user to select the following test modes in order to perform sequential groups
of tests or individual tests.
a. MTS Manufacturing Mode - Used to automatically perform all 11 “Calibration Tests” and
“Verify Tests,” in sequence, listed in the “Test Selection” window. If a test is stopped or
aborted and then restarted, MTS will start from the beginning of the test sequence.
Note: In general, Manufacturing mode should not be used for service repairs. If a
“Calibration” test is required, it should be selected from the Single Test mode.
b. MTS Verify Mode - Used to perform all six “Verify Tests,” in sequence, listed in the “Test
Selection” window. If a test is stopped or aborted and then restarted, MTS will start from
the beginning of the test sequence.
c. MTS Single Test Mode - Used to perform one or more MTS tests selected from the “Test
Selection” window. The selected tests are then automatically executed in sequence.
Each individual MTS test will be described below as performed from the Single Test function in
the order that it appears in the “Test Selection” window. Screens associated with the Verify
function will be noted as applicable. If a test is aborted or canceled, the program returns to the
“Device Select” screen where the user must then click on the “Find Reader” button and then
select the Auto-reader from the drop-down list to reconnect. The same tests can then be
repeated. Output “xml” test result data files are created and stored in the “c:\MTS\Tests” folder.
10.8.1. Select “Single Test” from the “Function” box.
Select
Figure 10.49 - MTS Main Screen – Single Test

10-25 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.2. Check the box next to a test from the “Calibration Test” or “Verify Test” sections and
then press the “Start” button to initiate the test.
Select
Figure 10.50 - MTS Single Test Selection Window
10.8.3. Calibration Tests
10.8.3.1. Real Time Clock Test
10.8.3.1.1. Check the “Real Time Clock” box in the MTS “Single Test - Test Selection”
window and then press the “Start” button.
10.8.3.1.2. Click the “Check RTC” button.
Click
Figure 10.51 - MTS Single Test - Real Time Clock Check Window
10.8.3.1.3. Verify the “RTC passed,” message, then click the “OK” button.
Verify
Click
Figure 10.52 - MTS Single Test - Real Time Clock Passed Window

78-8083-3058-9, Rev C 10-26
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.2. Battery Voltage Test
10.8.3.2.1. Remove the Auto-reader upper housing.
10.8.3.2.2. Measure the “Main Board” coin cell battery voltage with the Digital Multi-
meter.
Figure 10.53 - MTS Single Test – Battery Voltage Test Points
10.8.3.2.3. Verify the battery voltage is in the range 2.6 - 3.4 V.

If the voltage is below 2.6 V, replace the battery.
10.8.3.2.4. Reassemble the Auto-reader upper housing.
10.8.3.2.5. Check the “Battery Voltage” box in the MTS “Single Test - Test Selection”
10.8.3.2.6. Enter the measured voltage value in the “Battery Voltage” text box and then
click the “Continue” button.
Enter Voltage
Measurement
Click
Figure 10.54 - MTS Single Test – Battery Measurement Window

10-27 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.2.7. Click the “OK” button.
Click
Figure 10.55 - MTS Single Test – Battery Passed Window

78-8083-3058-9, Rev C 10-28
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.3. BID Calibration Test
10.8.3.3.1. Check the “BID Calibration” box in the MTS “Single Test - Test Selection”
10.8.3.3.2. Remove all objects from incubator wells.
10.8.3.3.3. Click the “Calibrate” button.
Click
Figure 10.56 - MTS Single Test – BI Detection Calibration Window
10.8.3.3.4. Verify the incubator wells are empty and then press the “OK” button.
Click
The BI Detection will run and then display the new calibration settings in the “BI
Detection Calibration” window.

10-29 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.3.5. Verify the BI Detection Calibration test passed and then press the “OK”
button.
If the BI Detection test fails, identify the out-of-specification well, repair the
failed well, and then re-run the test.
Click
10.8.3.3.6. Click the “Continue” button.
Click

78-8083-3058-9, Rev C 10-30
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.4. VEM Calibration Test
10.8.3.4.1. Check the “VEM Calibration” box in the MTS “Single Test - Test Selection”
10.8.3.4.2. Insert the calibration standards into the incubator wells and then select the
corresponding standard from the “Standards” drop-down list for each well.
(Note: Select the menu item “Change Standards Selection” (Password = Attest)
to enable the drop down list. If there are insufficient standards to fill all wells,
select “Skip Calibration” for the empty wells. After the wells with standards have
been calibrated, move the standards to the remaining wells, and repeat the
standards selection and calibration test.)
Select Standard
Click
Figure 10.60 - MTS Single Test – VEM Calibration Window
10.8.3.4.3. Click the “Calibrate” button.
10.8.3.4.4. The standards will undergo a warm-up period before the calibration test
begins. Calibration test progress status is displayed in the “Integration
Calibration Results” column.
Test Progress Status
Figure 10.61 - MTS Single Test – VEM Calibration In-process Window

10-31 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.4.5. Verify all wells were calibrated with passing results as displayed in the
“Integration Calibration Results” column and then click on the “Continue”
button.
(Note: If a well does not pass, re-do the test with another standard to verify the
failure. If the second test fails, repair the failed well.)
Verify all “Pass”
Click
Figure 10.62 - MTS Single Test – VEM Calibration Complete Window
10.8.3.4.6. Remove the calibration standard(s) from all wells and store them properly.

78-8083-3058-9, Rev C 10-32
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.5. Heater Block Calibration Test
The heater block can be adjusted by +/- 2°C. If adjustment is required, adjust the offset
so that the probe temperature reads within the following ranges:
Auto-reader Temperature Range

390 ..................60.0°C – 60.5°C
390G.................37.0°C – 37.5°C
490 ..................56.5°C – 57.0°C
490H.................60.0°C – 60.5°C
10.8.3.5.1. Check the “Heater Block Calibration” box in the MTS “Single Test - Test
Selection” window and then press the “Start” button.
10.8.3.5.2. Insert the temperature probe into the incubator block temperature test port
between wells 5 and 6.
Temperature
Test Port
Figure 10.63 - Temperature Calibration Measurement Setup
10.8.3.5.3. Allow the temperature probe meter reading to stabilize.

10-33 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.3.5.4. Click on either the green “up” arrow to increase the incubator block
temperature or the red “down” arrow to decrease the incubator block
temperature, if required, until the temperature is within the specified
temperature range. Allow the temperature probe meter reading to stabilize
after each adjustment.
Click to increase temp
Click Click to decrease temp

(If temp offset Click
modified)
Figure 10.64 - MTS Single Test – Temperature Calibration Window
10.8.3.5.5. Click the “Update” button if an adjustment was performed.
10.8.3.5.6. Click the “Continue” button.
10.8.3.5.7. Remove the temperature probe from the incubator block temperature test
port.

78-8083-3058-9, Rev C 10-34
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4. Verification Tests
10.8.4.1. Button Check
10.8.4.1.1. Check the “Button Check” box in the MTS “Single Test - Test Selection”
10.8.4.1.2. Complete the “Mute Button,” “Temperature Button,” and “Information Button”
tasks as instructed on the “Button Tests” screen and then click “Continue.”
Select
Select
Select
Select
Figure 10.65 - MTS Single Test – VEM Calibration Window

10-35 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.2. Alarm Check
10.8.4.1.1. Check the “Alarm Check” box in the MTS “Single Test - Test Selection”
10.8.4.1.2. Complete the “Audio Alarm” task as instructed on the “Alarm Test” screen
and then click “Continue.”
Select
Select
Figure 10.66 - MTS Single Test – Alarm Test Window

78-8083-3058-9, Rev C 10-36
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.3 Firmware Check
10.8.4.3.1. Check the “Firmware Check” box in the MTS “Single Test - Test Selection”
10.8.4.3.2. Complete the “Firmware Check” task as instructed on the “Firmware Version
Check” screen and then click “Continue.”
Firmware Select
Version
Loaded
Select
Figure 10.67 - MTS Single Test – Firmware Version Check Window
(Note: Check the HCSC website (www.3m.com/hcsc) to verify the latest firmware
version. If a newer version is found on the HCSC website, download and install it
into the Auto-reader.)
10.8.4.3.3. Remove the Auto-reader “Upper Housing” and verify the firmware listed on
the “Main Board” firmware version label is the same as the firmware version
listed in the “Firmware Version Check” test window.
Firmware
Version
Label
Figure 10.68 - Main Board - Firmware Version Label

10-37 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.4. Temperature Check
The heater block temperature must be within +/- 2°C of the target temperature. The
probe temperature measurement must be within the following ranges:
Auto-reader Target Temperature Temperature Range

390 ................... 60.0°C...................58.0°C – 62.0°C
390G.................. 37.0°C...................35.0°C – 39.0°C
490 ................... 56.5°C ..................54.5°C – 58.5°C
490H.................. 60.0°C ..................58.0°C – 62.0°C
10.8.4.4.1. Verify one of the following two conditions has been met before performing the
temperature check.
10.8.4.4.1.1. Auto-reader has been powered for a minimum of 30 minutes prior to

verification and the board temperature meets the minimum
temperature requirement.
Auto-reader Main Board Temperature

390........................ > 40.0°C
390G ...................... > 33.0°C
490........................ > 38.0°C
490H....................... > 40.0°C
10.8.4.4.1.2. A two hour warm-up period has elapsed since power-on.
(Note: If the warm-up requirements have not been met when performing a “Verify”
sequence, a “Warm-up Requirements Have Not Been Met” window will display;
the window will not display during a “Single Test.”)
Warm-up
Status
Figure 10.69 - MTS Verify Sequence – Warm-up Requirements Not Met Window (390)

78-8083-3058-9, Rev C 10-38
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.4.2. Check the “Temperature Check” box in the MTS “Single Test - Test
10.8.4.4.3. Insert the temperature probe into the incubator block temperature test port
between wells 5 and 6.
Temperature
Test Port
Figure 10.70 - Temperature Measurement Setup
10.8.4.4.4. Complete the “Temperature Check” task as instructed on the “Temperature

Measurement” screen and then click “Continue.”
Enter
Temperature
Measurement
Value
Select
Figure 10.71 - MTS Single Test – Temperature Measurement Window

10-39 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.4.5. Click the “OK” button when the “Temperature Passed” window appears.
Click
Figure 10.72 - MTS Single Test - Temperature Passed Window

10.8.4.4.6. Remove the temperature probe from the incubator block temperature test
port.

78-8083-3058-9, Rev C 10-40
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5. BI Insertion
The “BI Insertion” test is performed on empty wells and with inserted BIs in all ten wells.
Each model Auto-reader is tested with a different BI as shown below:
Auto-reader BI Cap Color

390 ............... 1292 ............. Brown
390G .............. 1294 ............. Green
490 ..............1492V ............ Brown
490H .............1492V ............ Brown
The test screens are slightly different between the 390/390G and 490/490H models.
“Empty well” and “closed cap” tests are performed on all Auto-readers, but the “open
cap” test is only performed on the Models 490 and 490H Auto-readers.
Accordingly, the test sequence below is separated into a “Model 390/390G” section and
a “Model 490/490H” section.
10.8.4.5.1. Check the “BI Insertion” box in the MTS “Single Test - Test Selection”
10.8.4.5.2. Model 390/390G
10.8.4.5.2.1. Verify all wells are empty and then click the “BID Read Empty Wells”
button.
Select
Figure 10.73 - MTS Single Test – BI Insertion Test Window

10-41 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.2.2. Verify all wells passed and then click the “OK” button.
Click
Figure 10.74 - MTS Single Test – BID Read Empty Well Subtest Window
10.8.4.5.2.3. Insert a closed cap BI in every well and then click the “BID Read
Closed Cap” button.
Click
Click
Figure 10.76 - MTS Single Test – BID Read Closed Cap Subtest Window

78-8083-3058-9, Rev C 10-42
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.2.5. Remove all BIs from the wells.
10.8.4.5.2.6. Click the “Continue” button.
Click

10-43 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.3. Model 490/490H
10.8.4.5.3.1. Verify all wells are empty and then click the “BID Read Empty Wells”
button.
Select
Click
Figure 10.79 - MTS Single Test – BID Read Empty Well Subtest Window

78-8083-3058-9, Rev C 10-44
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.3.3. Insert an open cap BI in every well and then click the “BID Read
Open Cap” button.
Click
Click
Figure 10.81 - MTS Single Test – BID Read Open Cap Subtest Window

10-45 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.3.6. Insert a closed cap BI in every well and then click the “BID Read
Closed Cap” button.
Click
Click
Figure 10.83 - MTS Single Test – BID Read Closed Cap Subtest Window

78-8083-3058-9, Rev C 10-46
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.5.3.9. Click the “Continue” button.
Click

10-47 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.6. Fluorescent Detection
10.8.4.6.1. Check the “Fluorescent Detection” box in the MTS “Single Test - Test
10.8.4.6.2. Remove all objects from incubator wells.
10.8.4.6.3. Insert the calibration standards into the incubator wells and then select the
corresponding standard from the “Standards” drop-down list for each well.
(Note: Select the menu item “Change Standards Selection” (Password =

Attest) to enable the drop down list. If there are insufficient standards to fill
all wells, select the standards that will be used in the empty wells. After the
wells with standards have been tested, move the standards to the empty
wells and then click the “Perform Test” button.)
Click
Figure 10.85 - MTS Single Test – Fluorescent Detection Window
10.8.4.6.4. Click the “Perform Test” button.
10.8.4.6.5. The standards will undergo a warm-up period before the fluorescent
detection test commences. Test progress status is displayed in the “Status”
box and test results are displayed in the “Notes” box.

78-8083-3058-9, Rev C 10-48
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.6.6. Verify all wells were tested and then click the “Continue” button.
Click
Figure 10.86 - MTS Single Test – Fluorescent Detection Window
10.8.4.6.7. Remove the calibration standard(s) from all wells and store them properly.
10.8.4.6.8. MTS will return to the Main Screen.
10.8.4.6.9. Verify Sequence Results (applicable if “Verify” function was selected from the
Main Screen instead of the “Single Test” function).
10.8.4.6.9.1. An MTS window will display the message “Entire test sequence
completed successfully” if the test was successful. Click the “OK”
button to continue.
Click
Figure 10.87 - MTS – Verify Test Sequence Completed Successfully Window

10-49 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.8.4.6.9.2. Inspect the “xml” test results file located in the “c:\MTS\Test” folder
and verify all six verification tests passed (e.g.
“Attest_100170_02_09_2015_15_31_08_VER_Pass.xml”).
Verify
Figure 10.88 - MTS – Verify “xml” Test Results File
10.8.4.6.9.3. Fluorescent measurement sensitivity test data for a “Certificate of

Calibration”, if required, is located in the “xml” file under the
“Fluorescent Detection Test” section
Verify
Figure 10.89 - MTS – “xml” File Fluorescent Measurement Sensitivity Test Data

78-8083-3058-9, Rev C 10-50
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.9 Serial Number Update

10.9.1. The MTS Serial Number function is used to modify a main board default serial number to
the original Auto-reader serial number after a “Main Printed Circuit Board Assembly”
replacement. MTS login is not required to access Serial Number. A connected Auto-
reader is required to access the Serial Number function.
10.9.1.1. Select > “Serial Number” from the MTS Main Screen, then click the “Start” button.
Select
Click
Figure 10.90 - MTS Main Screen – Serial Number Function
10.9.1.2. Click the “Yes” button in the “New Serial Number Confirmation” window.
Click
Figure 10.91 - MTS Serial Number Function – New Serial Number Confirmation Window
10.9.1.3. Click the “Yes” button in the “New Serial Number Confirmation” window.
Click
Figure 10.92 - MTS Serial Number Function – Manual Serial Number Confirmation Window

10-51 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
10.9.1.4. Enter the Auto-reader serial number in the “Serial#” text box, then click the
“Program Serial #” button.
Enter serial number
Click
Figure 10.93 - MTS Serial Number Function – Prepare Device Window
10.9.1.5. Click the “OK” button when the new serial number programming is complete.
Click
Figure 10.94 - MTS Serial Number Function – Program Complete
10.9.1.6. Verify the S/N displayed on the front panel display is the desired S/N.

78-8083-3058-9, Rev C 10-52
Status: Release
Release Date:4/15/2015 8:15:12 PM
11.0 MECHANICAL DRAWINGS AND PARTS LIST

11.1. Final Assembly - Illustrated Parts List
8
14 Note:
6, 7, 8
2, 4,
5
3
Note: 1, 2, 3, 4
11
7 Note: 5
12
13
15
9
Power Adapter-Argentina
16
(Image Not Shown)
10
Figure 11.1 - Final Assembly
© 3M 2015, All rights reserved.

11-1 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
Final Assembly – Parts List
Spare
Item Part Number Description 390 390G 490 490H Notes*
Part
1 26-1017-2710-0 SCREW,METRIC,CAP,M3X6,HEX SKT, SST 6 6 6 6 x ---
2 26-1017-8621-3 SCREW, TORX, 3X6, THREAD CUTTING 10 10 10 10 x ---
3 26-1021-2361-4 CONNECTOR, NICOMATIC, HOUSING, 4 POS 1 1 1 1 x 1, 2, 3, 4
4 78-0005-0367-8 HOUSING, LOWER 1 1 1 1 x ---
5 78-8083-2561-3 390/390G HOUSING, UPPER 1 1 --- --- N/A 1, 2
78-0005-0368-6 HOUSING, UPPER --- --- 1 1 N/A 3, 4
6 78-0005-0369-4 DISPLAY LENS 1 1 1 1 N/A 1, 2, 3, 4
7 78-8083-2567-0 390 BI BLOCK/PCBA ASSEMBLY 1 --- --- --- N/A 5
78-8083-2568-8 390G BI BLOCK/PCBA ASSEMBLY --- 1 --- --- N/A 5
78-0005-0370-2 490 BI BLOCK/PCBA ASSEMBLY --- --- 1 --- N/A 5
78-8083-3460-7 490H BI BLOCK/PCBA ASSEMBLY --- --- --- 1 N/A 5
8 78-8083-2466-5 390 BUTTON OVERLAY ASSEMBLY 1 --- --- --- N/A 1
78-8083-2467-3 390G BUTTON OVERLAY ASSEMBLY --- 1 --- --- N/A 2
78-0005-0380-1 490 BUTTON OVERLAY ASSEMBLY --- --- 1 --- N/A 3
78-8083-3181-9 490H BUTTON OVERLAY ASSEMBLY --- --- --- 1 N/A 4
9 78-0005-0381-9 FOAM PAD, BACK 1 1 1 1 x ---
10 78-0005-0382-7 FOAM PAD, FRONT 1 1 1 1 x ---
11 78-0005-0388-4 RIBBON CABLE ASSEMBLY 1 1 1 1 x ---
12 78-0005-0394-2 LCD DISPLAY ASSEMBLY 1 1 1 1 x ---
13 78-8083-2565-4 390 SERIAL PLATE LABEL 1 --- --- --- N/A ---
78-8083-2566-2 390G SERIAL PLATE LABEL --- 1 --- --- N/A ---
78-0005-0397-5 490 SERIAL PLATE LABEL --- --- 1 --- N/A ---
78-8083-3183-5 490H SERIAL PLATE LABEL --- --- --- 1 N/A ---
14 78-0005-0384-3 BI CAP LABEL - BROWN 1 --- 1 --- N/A 6
78-0005-0385-0 BI CAP LABEL - BLUE 1 --- 1 --- N/A 6
78-0005-0386-8 BI CAP LABEL - BLUE/BROWN 1 --- 1 --- N/A 6
78-8083-2575-3 BI CAP LABEL - GREEN --- 1 --- --- N/A 2, 7
78-8083-3182-7 BI CAP LABEL - PINK --- --- --- 1 N/A 4, 8
15 78-0005-0417-1 POWER SUPPLY 1 1 1 1 x ---
MULTI-BLADE POWER ADAPTOR FOR
16 26-1021-4266-3 1 1 1 1 x ---
ARGENTINA
* Spare parts available as part of a kit or subassembly

Spare
Note Part Number Description 390 390G 490 490H Item
Part
SPARE PARTS - 390 UPPER HOUSING W/ITH
1 78-8083-3188-4 1 --- --- --- x 3, 5, 6, 8
390 BUTTON OVERLAY
SPARE PARTS - 390G UPPER HOUSING WITH
2 78-8083-3189-2 --- 1 --- --- x 3, 5, 6, 8, 14
390G BUTTON OVERLAY
SPARE PARTS - 490 UPPER HOUSING WITH
3 78-8083-3190-0 --- --- 1 --- x 3, 5, 6, 8
490 BUTTON OVERLAY
SPARE PARTS - 490H UPPER HOUSING WITH
4 78-8083-3967-1 --- --- --- 1 x 3, 5, 6, 8, 14
490H BUTTON OVERLAY
5 --- See Illustrated Parts List-BI Block/PCB Assembly 1 1 1 1 x 7
6 78-8083-3364-1 STEAM BI LABEL, SPARE PARTS KIT 1 --- 1 --- x 14
7 78-8083-3365-8 EO BI LABEL, SPARE PARTS KIT --- 1 --- --- x 14
8 78-8083-3968-9 490H LABEL, SPARE PARTS KIT --- --- --- 1 x 14

78-8083-3058-9, Rev C 11-2
Status: Release
Release Date:4/15/2015 8:15:12 PM
11.2. BI Block / PCB Assembly - Illustrated Parts List
(22) 2
10
7
4
3
(10) 14
11
12
9
15 (10)
16
13 (10)
1 (6)
Note: 1, 2
Figure 11.2 - BI Block / PCB Assembly

11-3 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
BI Block / PCB Assembly - Parts List
Spare
Item Part Number Description 390 390G 490 490H Notes*
Part
1 26-1017-2055-0 SCREW, SOCKET CAP, M2 X 6 MM, SS 6 6 6 6 X ---
2 26-1017-8621-3 SCREW, TORX, 3X6, THREAD CUTTING 22 22 22 22 X ---
3 26-1017-8631-2 PARLEX FLAT CABLE, 100R14-76B, 3" 1 1 1 1 X ---
4 26-1017-8632-0 PARLEX FLAT CABLE, 100R14-51B, 2" 1 1 1 1 X ---
5 78-8083-2570-4 390 INCUBATOR BLOCK HOUSING 1 1 --- --- X ---
78-0005-0351-2 490 INCUBATOR BLOCK HOUSING --- --- 1 1 X ---
6 78-0005-0352-0 OPTICS ALIGNMENT BLOCK 1 1 1 1 X ---
7 78-0005-0353-8 OPTICS ALIGNMENT BLOCK COVER 1 1 1 1 X ---
8 78-8083-2569-6 390 INCUBATOR BLOCK 1 1 --- --- N/A 1
78-0005-0354-6 490 INCUBATOR BLOCK --- --- 1 1 N/A 2
9 78-8083-2571-2 390 PCB ASSEMBLY, MAIN 1 --- --- --- X ---
78-8083-2572-0 390G PCB ASSEMBLY, MAIN --- 1 --- --- X ---
78-0005-0355-3 490 PC BOARD ASSEMBLY, MAIN --- --- 1 --- X ---
78-8083-3461-5 490H PC BOARD ASSEMBLY, MAIN --- --- --- 1 X ---
10 78-0005-0358-7 PC BOARD ASSEMBLY, LED 1 1 1 1 X ---
11 78-0005-0361-1 PC BOARD ASSEMBLY, SENSOR 1 1 1 1 X
12 78-0005-0371-0 HEATER FLEX ASSEMBLY 1 1 1 1 N/A 1, 2
13 78-8083-2656-1 GLASS FILTER, EMISSION, LIGHT BLUE 1 1 1 1 X ---
14 78-8083-2657-9 GLASS FILTER, EXCITATION, BLACK 1 1 1 1 X ---
15 78-0005-0379-3 GEL FILTER, EMISSION 1 1 1 1 X ---
16 78-0005-0383-5 INCUBATOR BLOCK INSULATOR 1 1 1 1 N/A 1, 2
* Spare parts available as part of a kit or subassembly

Spare
Note Part Number Description 390 390G 490 490H Item
Part
SPARE PARTS - 390/390G INCUBATOR
1 78-8083-3191-8 1 1 --- --- x 8, 12, 16
BLOCK W/ FLEX HEATER AND INSULATOR
SPARE PARTS - 490/490H INCUBATOR
2 78-8083-3192-6 --- --- 1 1 x 8, 12, 16
BLOCK W/ FLEX HEATER AND INSULATOR

78-8083-3058-9, Rev C 11-4
Status: Release
Release Date:4/15/2015 8:15:12 PM
11.3. Calibration Standards – Illustrated Parts List
Figure 11.3 - Calibration Standard Sets
Item Part Number Description 390 390G 490 490H

1 78-8083-3123-1 390/390G CALIBRATION SET 1 1 --- ---
2 78-0005-0404-9 490 CALIBRATION SET --- --- 1 1

11-5 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
12.0. ELECTRICAL SCHEMATICS
12.1. Main Board Schematic (78-0005-0357-9, Sheet 1 of 3)
Figure 12.1 - Schematic, Main Board (Sheet 1 of 3)

78-8083-3058-9, Rev C 12-1
Status: Release
Release Date:4/15/2015 8:15:12 PM
Main Board Schematic (78-0005-0357-9, Sheet 2 of 3)

12-2 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
Main Board Schematic (78-0005-0357-9, Sheet 3 of 3)

78-8083-3058-9, Rev C 12-3
Status: Release
Release Date:4/15/2015 8:15:12 PM
12.2. Sensor Board Schematic (78-0005-0363-7)
Figure 12.4 - Schematic, Sensor Board

12-4 78-8083-3058-9, Rev C
Status: Release
Release Date:4/15/2015 8:15:12 PM
12.3. LED Board Schematic (78-0005-0360-3)
Figure 12.5 - Schematic, LED Board

78-8083-3058-9, Rev C 12-5
Status: Release
Release Date:4/15/2015 8:15:12 PM
3M Health Care
3M Health Care Service Center
Suite 200, Building 502
3350 Granada Ave N
Oakdale, MN 55128
U.S.A.
1-800-292-6298
www.3M.com/infectionprevention
3M Health Care
3M Canada
Post Office Box 5757
London, Ontario N6A4T1
Canada
1-800-364-3577
www.3M.com/ca/healthcare
3M Deutschland GmbH
Health Care Business
Cari-Schurz-Str.-1
41453 Neuss, Germany

12-6 78-8083-3058-9, Rev C

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3M Confidential: 3M™ Attest™ Auto-Reader Service Manual Models 390, 390G, 490, 490H

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3M Confidential Manual-Service-05-207096

Document Name: Manual-Service-05-207096

Document Title: 390/390G/490/490H Auto-reader Service Manual

Effective From: 4/15/2015 8:15:12 PM To:

Owner: Schenk Stephen B, A1GY7ZZ

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3M™ Attest™ Auto-reader

Model 390 Model 390G

Model 490 Model 490H

© 3M 2015. All rights reserved.

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© 3M 2015. All rights reserved.

© 3M 2015. All rights reserved.

© 3M 2015. All rights reserved.

Environmental Operating Conditions

© 3M 2015. All rights reserved.

2.0 PRODUCT DESCRIPTION / INTENDED USE

3M™ Attest™ Auto-reader Model 390

A positive fluorescence reading or a visual pH color change indicates a steam sterilization

© 3M 2015. All rights reserved.

3M™ Attest™ Auto-reader Model 390G

© 3M 2015. All rights reserved.

3M™ Attest™ Auto-reader Model 490

© 3M 2015. All rights reserved.

3M™ Attest™ Auto-reader Model 490H

© 3M 2015. All rights reserved.

3.0 SAFETY WARNINGS AND CAUTIONS

Explanation of Single Word Consequences

Warnings and Safety Precautions

WARNING: To reduce the risk associated with hazardous voltage.

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CAUTION: To reduce the risk of injury or instrument damage.

Always unplug the Auto-reader and allow to cool before cleaning.

The instrument must be returned to the manufacturer for repair.

WARNING: To reduce the risk associated with incorrect results.

CAUTION: To reduce the risk associated with incorrect results.

© 3M 2015. All rights reserved.

3.1 Device Safety Compliance

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3.2 EMC Compliance

 EMC requirements of the CE mark EMC Directive 2004/108/EC.

© 3M 2015. All rights reserved.

3.3 Explanation of Product and Packaging Label Symbols

Attention - Refer to the instructions for use.

Waste Electrical and Electronic Equipment (WEEE) and EU Battery Directive

UL Listed to US and Canadian Safety Standards.

Mark of Conformity to European Directives.

Compliant to all applicable ACMA regulatory arrangements (RCM)

Manufacturer – This symbol is accompanied by name and address of the

Catalogue – This symbol is accompanied by the catalogue number relevant to the

Authorized representative for the European Community – This symbol is