TProfessional

Thermocycler
Family

Service manual

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TProfessional
Service manual

10 / 2007

Biometra
biomedizinische Analytik GmbH
Rudolf-Wissell-Str. 30, D-37079 Goettingen
Tel.: 0551/50 686-0; Fax: 0551/50 686-66
email:.info@biometra.com
www.biometra.com

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Index___________________________________________________________________________ I

Preface

This Service Manual is written as complete and exact as possible. Far as lawful authorised we exclude
any liability and consequential damages.

Biometra biomedizinische Analytik GmbH believes the information contained in this manual is accurate
and reliable at time of print. Biometra reserves the right to modify any information contained in the
manual without prior notice. Biometra is not liable for inadvertent errors or omissions.

We must reserve the right to modify the design every time. Copyright owner for text and creation is

© Biometra biomedizinische Analytik GmbH

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1 Introduction........................................................................................................................... 2
1.1 TProfessional Thermo block........................................................................................... 2
1.2 TProfessional Lid............................................................................................................ 2
1.3 TProfessional User interface........................................................................................... 2
1.4 TProfessional Housing.................................................................................................... 2
2 Safety precautions ................................................................................................................. 3
3 Installation ............................................................................................................................. 4
3.1 Content of delivery.......................................................................................................... 4
3.2 Operation Voltage ........................................................................................................... 4
3.3 Setting up the TProfessional ........................................................................................... 5
3.4 Initial self test (power on self test).................................................................................. 5
4 Getting familiar with the TProfessional.............................................................................. 6
4.1 TProfessional Thermocycler front view ......................................................................... 6
4.2 TPROFESSIONAL Thermocycler rear view ....................................................................... 6
4.3 Exchange of TProfessional block module ...................................................................... 7
4.4 The TProfessional control panel ..................................................................................... 7
4.5 High Performance Smart Lid (HPSL)............................................................................. 8
4.6 Releasing blocked lid wheel ........................................................................................... 8
5 The TProfessional User Interface...................................................................................... 10
5.1 TProfessional Menu Buttons......................................................................................... 10
5.2 TProfessional Soft Keys ............................................................................................... 10
5.3 Log in Menu.................................................................................................................. 11
5.4 Block Menu................................................................................................................... 11
5.5 System Menu ................................................................................................................ 12
5.6 Program Menu............................................................................................................... 12
5.7 Navigation within software menus ............................................................................... 13
6 Login In................................................................................................................................ 13
6.1 Log in existing User...................................................................................................... 14
6.2 Create new user account ............................................................................................... 15
6.3 Supervisor Log in.......................................................................................................... 16
6.4 Delete User.................................................................................................................... 17
7 Create program .................................................................................................................. 18
7.1 Set Block Type.............................................................................................................. 18
7.2 Heated lid settings......................................................................................................... 19
7.2.1 Set lid pre-heating mode ....................................................................................... 19
7.2.2 Set lid temperature ................................................................................................ 20
7.3 Enter step temperature and time.................................................................................... 20
7.4 Set loop ......................................................................................................................... 21
7.5 Cool below ambient temperature .................................................................................. 22
7.6 Program Pause .............................................................................................................. 22
7.7 Save program ................................................................................................................ 22
8 Set Gradient......................................................................................................................... 25
9 Edit programs...................................................................................................................... 26
9.1 Insert program step........................................................................................................ 26
9.2 Delete program step ...................................................................................................... 27
9.3 Copy program ............................................................................................................... 29
9.4 Delete program.............................................................................................................. 30

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9.5 Editing programs in the graphical mode .......................................................................31

10 Change program options ....................................................................................................33
10.1 Heating and cooling rate ...............................................................................................33
10.2 Time increment Æ Δt(s)................................................................................................34
10.3 Temperature increment or decrement Æ ΔT(°C)..........................................................34
11 Run program .......................................................................................................................36
11.1 Select program for start from User directories..............................................................36
11.2 Quick start programs from the block Menu ..................................................................36
11.2.1 View program prior to start...................................................................................37
11.3 Display during operation...............................................................................................38
11.4 Pause Program during run .............................................................................................39
11.5 Continue program..........................................................................................................39
12 Stop program .......................................................................................................................40
12.1 Stop Program from a pause ...........................................................................................40
13 System settings.....................................................................................................................41
13.1 User configuration.........................................................................................................41
13.1.1 Set language ..........................................................................................................41
13.1.2 Change individual PIN ..........................................................................................42
13.1.3 Delete Account......................................................................................................42
13.2 System configuration.....................................................................................................42
13.2.1 Configure beep ......................................................................................................43
13.2.2 Configure Contrast ................................................................................................43
13.2.3 Set time and date ...................................................................................................44
13.3 System info....................................................................................................................44
13.3.1 System info............................................................................................................44
13.3.2 View Log files of the last five runs .......................................................................45
13.4 Service...........................................................................................................................46
13.4.1 View log files of Power on self test ......................................................................46
13.4.2 Execute extended self test .....................................................................................46
13.4.3 View log files from extended self test...................................................................47
13.4.4 View Error log files...............................................................................................47
13.4.5 Create Info file for Biometra Service Department ................................................47
13.5 How to contact Biometra...............................................................................................47
14 Maintenance.........................................................................................................................48
14.1 Cleaning and Decontamination .....................................................................................48
14.2 Trouble shooting ...........................................................................................................48
14.2.1 Slow heating and cooling ......................................................................................48
14.2.2 Restart due to unrecognised power failure............................................................48
14.2.3 Adaptation of protocols from other cyclers...........................................................48
14.2.4 Releasing wheel in case of blocked lid .................................................................48
15 Service and repair ...............................................................................................................49
15.1 Spare parts .....................................................................................................................49
15.2 Assembling instructions ................................................................................................49
15.2.1 Main groups...........................................................................................................50
15.2.1.1 Air baffle (9-020-010)...................................................................................50
15.2.1.2 Fan chassis (9-020-011) ................................................................................50
15.2.1.3 Back cover plate (9-020-012)........................................................................51
15.2.1.4 Housing complete (9-014-001) .....................................................................52
15.2.1.5 Fixed modules (Tprofessional STANDARD; TProfessional BASIC)..........53

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15.2.2 Housing ................................................................................................................. 55

15.2.2.1 Housing and display (7-014-071; 9-073-022)............................................... 55
15.2.2.2 Keypad foil (7-076-019) ............................................................................... 56
15.2.3 Heated lid .............................................................................................................. 56
15.2.3.1 Heated lid seal (7-014-081)........................................................................... 56
15.2.3.2 Connection of heated lid ............................................................................... 57
15.2.3.3 Lid plug ......................................................................................................... 57
15.2.3.4 Knob (9-010-033) ......................................................................................... 58
15.2.3.5 Planetary gear (9-010-034) ........................................................................... 58
15.2.3.6 Fixing of heating element; TProfessional 60 (9-010-007),
96 (9-010-006) and 384 (9-010-008) ............................................................ 59
15.2.3.7 Spring plate (9-010-035)............................................................................... 59
15.2.4 Chassis .................................................................................................................. 60
15.2.4.1 Power board (9-070-013) .............................................................................. 60
15.2.4.2 Processor board (9-070-014)......................................................................... 63
15.2.4.3 Display I/O board (9-070-012) ..................................................................... 65
15.2.5 Back cover plate.................................................................................................... 66
15.3 Wiring diagram ............................................................................................................. 67
15.3.1 Main Board description......................................................................................... 68
15.3.2 Processor Board description.................................................................................. 68
15.3.3 Display I/O Board discription ............................................................................... 68
15.4 Search Fault Trees......................................................................................................... 69
15.4.1 Fault tree “Bolck” ................................................................................................. 69
15.4.2 Fault tree “Display” .............................................................................................. 70
15.5 Firmware update ........................................................................................................... 70
15.6 Service repair form........................................................................................................ 71
16 Instructions for return shipment ....................................................................................... 72
17 Equipment Decontamination Certificate .......................................................................... 73
18 Technical specification........................................................................................................ 74
19 Warranty.............................................................................................................................. 75
20 Declaration of Conformity ................................................................................................. 76
21 Note for the disposal of electric / electronical waste. ....................................................... 77

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1 Introduction
The Biometra TProfessional Thermocycler is inspired by the wish to simplify molecular biology
research. To reach this goal the instrument housing, lid and block were totally redesigned,
providing so far unrealised speed and temperature uniformity. Innovative user software was
developed, driven by Biometras 15 years experience in interface design.

The result is an easy to use Thermocycler with excellent technical specifications.

1.1 TProfessional Thermo block

The TProfessional Thermocycler offers three different block modules. To achieve ultimate
performance the 96 well modules are made of Silver. Due to its excellent heat conductivity
Silver equilibrates extremely quickly, thus providing maximum speed and temperature
uniformity. To protect the valuable silver blocks against corrosion, the block surfaces are Gold
plated.
The 96 well silver block is designed for daily routine work and can be used with 0.2 ml tubes,
strips or microplates. For rapid optimisation of new protocols, a 96 well Gradient version is
available. The third block module is designed for use with 384 well plates. All block modules
can be easily exchanged in less than 10 seconds.

1.2 TProfessional Lid

The TProfessional lid has been optimised to achieve two essential requirements: prevention of
condensation and reliable contact between samples and thermoblock. The lid is powered by
Biometras well know smart lid technology which automatically limits lid pressure to save tubes
from damage. In addition, the new design of the High Performance Lid ensures an even
temperature distribution between samples thus significantly improving temperature uniformity.
With one press on the front button, the lid gently swings open and arrests in its end position.

1.3 TProfessional User interface

The TProfessional user interface incorporates Biometras proven spreadsheet-programming

philosophy. Avoiding endless serial program windows, all steps of a program are entered in a
single screen. This makes the creation of new or editing existing programs fast and easy. One
touch leads from the spreadsheet to an alternative graphical programming mode. The
TProfessional manages up to 30 individual users (all password protected) and provides for a
large program store.

1.4 TProfessional Housing

The elegant TProfessional housing is designed for heavy use. Due to an optimised air stream
design even higher efficiency and better temperature uniformity is achieved. The instrument
works quietly and consumes little power, which in turn leads to low heat emission. The compact
footprint saves valuable bench space. Keyboard and display have been set to an angle that
ensures reflection-free viewing and ergonomic programming. To facilitate servicing the
TProfessional housing can be completely removed from the chassis.
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2 Safety precautions
Please read this manual carefully before starting operation of the TProfessional Thermocycler.
The TProfessional Thermocycler is intended for sample incubation at varying temperatures.

• General safety precautions for laboratory work must be observed when working with the
TProfessional thermocycler.
• Be aware that samples are reaching high temperatures. Do not touch or open hot tubes or
microplates, because hot liquid may quickly spill out.
• Do not heat samples without having the lid locked securely.
• Before opening of the lid, release lid pressure (see section 4.5)
• Do not place fingers between lid and housing when opening or closing the lid.
• Do not touch the heated lid.
• When only few samples are put in the block place additional tubes in the four corner
positions. This is to evenly distribute the lid pressure and prevents single tubes from
excessive pressure. Use of few tubes may result in damage of the tubes by excessive
pressure.
• Use only suited plastic ware in the TProfessional thermocycler. Tubes and plates must show
good fit when placed in the thermoblock. Only use tubes that are suited for high temperatures
(tight lids).
• The TProfessional Thermocycler contains no user serviceable parts. Do not open the housing
instrument. Service and repair may only be carried out by the Biometra Service department
or otherwise qualified technical personal.
• Do not use the instrument when damages of the housing, block, cable or other parts are
visible.
• The TProfessional Thermocycler must not be used with explosive, flammable or volatile
liquids.
• Appropriate safety regulations must be observed when working with infectious or pathogenic
material.
• The Tprofessional does not produce a sound power level that could be hazardous for the
user.

The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.

Rapid heating of the thermoblock can cause liquids to boil

explosively. Always wear safety goggles during operation. Close the
lid before starting a program.

It is not necessary to apply oil into the opening of the block in order
to improve the heat transfer between the block and the sample tubes.

If you still decide to use oil, do not use silicon oil. Mineral oil may
be used.
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3 Installation

3.1 Content of delivery

1) Thermocycler with exchangeable block module

2) Mains connector
3) Manual
4) Short Manual

Please keep the original packaging material for return shipment in case of servicing. The
TProfessional shipping box provides a specially developed system for contact-free transport of
this electronic device.

3.2 Operation Voltage

Important: Prior to connecting the TProfessional to the mains, make sure that the setting of the
Voltage selector is in accordance with your mains Voltage.

The TProfessional Thermocycler can operate at 100, 115 or 230 Volt. The operation Voltage is
shown on the Voltage selector which is located at the instrument bottom.

To change operation Voltage of the TProfessional, switch off the instrument and disconnect the
mains plug.

STEP 1 Use a coin or another round shape item to turn the adjustment slot of the Voltage
selector to the new Voltage.

Instrument bottom Instrument backside

Voltage Mains plug

indicator

230 Fuses
5
11

Fuses
110

Adjustment
slot
1 Power switch
2

Important: After changing the operation Voltage, the instrument fuses have to be replaced. Fuse
specifications for each Voltage are shown on the type label on the instrument backside.

STEP 2: Insert Fuses according to selected Voltage at the backside of the instrument.
Service Manual TProfessional _______________________________________________________ 5

3.3 Setting up the TProfessional

• Place the TProfessional Thermocycler on a stable surface in a dry environment.

• Let equilibrate the TProfessional Thermocycler to room temperature before starting
operation (1 to 6h).
• Make sure that the appliance connector and the plug of the supply cord are accessible, so you
can separate the instrument from the mains.
• Make sure that the ventilation slots on the bottom and the rear are not obstructed (see section
4.2). Make sure that there is no object underneath the thermocycler that may block the
ventilation slots at the bottom (e.g. a piece of paper etc.)
• There must be sufficient distance between the ventilation slots on the rear side of the
Thermocycler and a wall or another instrument (min 10 cm).

Ensure that both the rear and bottom ventilation slits of the
rear and bottom of the instrument are unobstructed.

Insufficient ventilation can cause overheating of the

instrument.

• Make sure that the main supply voltage is in accordance with the label above the power
connection (see section 4.2)
• Connect the TProfessional Thermocycler to a grounded socket.
Prior to connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.

Danger of electric shock! Unplug the power cable before you open the
TProfessional Thermocycler.

• The display contrast can be adjusted to local lightning conditions (13.2.2)

3.4 Initial self test (power on self test)

After switching on the TProfessional serial number of the instrument and the software version is
displayed.

A log file of the power on self test is stored in the Thermocycler memory (see section 13.4.1).
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4 Getting familiar with the TProfessional

4.1 TProfessional Thermocycler front view

Lid pressure
adjustment wheel

Lid open
button

Display

Start / Stop Keypad

Button

4.2 TPROFESSIONAL Thermocycler rear view

R232 serial Port

Block release
lever

Mains plug Power on / off

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4.3 Exchange of TProfessional block module

Switch off the instrument.

Lift release lever on the backside (1) then pull block slowly to the back (2).

2
1

To install an new block module insert module carefully from the back in the base unit (2) until
the plugs at the front of the module are in contact with the socket inside the instrument. Then
carefully push down the release lever (1).

Switch on instrument. During boot up the block module (block format and serial number) is
automatically recognized.

4.4 The TProfessional control panel

Menue Buttons

Keypad
LED
Block
status

Start

Stop

Back Soft Keys Navigation

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4.5 High Performance Smart Lid (HPSL)

To achieve optimum pressure on the tubes the TPROFESSIONAL is equipped with a height
adjustable heated lid.

Close the lid:

After the samples have been placed in the block close the lid. Turn the wheel clockwise until you
hear a clicking noise. In this mode the pressure will not increase further, even when you keep on
turning the wheel.

Note: The pressure of the lid has been optimised for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.

Open the lid:

First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Open the lid with by pushing the front button.

Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.

4.6 Releasing blocked lid wheel

Note: When the lid is in the very up or down position, it may happen that the wheel is
uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in
either direction).

To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
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Release lid in upper position:

1) press pin
2) carefully turn wheel while holding
the pin down CLOCKWISE, until
you feel normal resistance (no more
clicking noise, clutch is released).
Release pin and turn lid down, until 1
the clutch mechanism is activated
(clicking noise, optimum pressure
applied). 2

Release lid in down position:

1) press pin
2) carefully turn wheel while holding
the pin down COUNTER
CLOCKWISE, until you feel
normal resistance (no more clicking
noise, clutch is released). Release pin
and turn wheel counter clockwise
until pressure is completely released.
Open lid.

Important: When the clutch mechanism is active (= optimum pressure is applied), do not use pin
to further increase lid pressure. This would lead to damage of tubes and instrument!
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5 The TProfessional User Interface

The TProfessional User interface provides four Menu Buttons above the screen and four Soft
Keys below the Screen,

Menu buttons

Softkeys

5.1 TProfessional Menu Buttons

The four MENU BUTTONS allow quick access to the TProfessional Main Menus. These are:

• Login /out Menu

• System Menu
• Block Menu
• Program Menu

The active menu is indicated by a graphical link to the respective menu button.

The example shows Program Menu is active.

The four Menu Buttons are permanent and always have the same function. This is in contrast to
the Soft Keys. The Soft Key functions change depending on the current software menu.

5.2 TProfessional Soft Keys

Below the Display there are four SOFT KEYS.

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In contrast to the Menu Buttons, the Soft Keys have changing functionalities, which are shown in
the above display line.

5.3 Log in Menu

To enter the Log In menu, press the Menu Button [Log in/out] above the display.

When the instrument is switched on, the Log in screen is displayed. To use the instrument a user
has to be selected. Alternatively, a new user can be created.

5.4 Block Menu

To enter the Block Menu, press the Menu Button [Block] above the display.
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The block menu shows the current status of the block. The block can be active with a program or
free.

If the block is free (not running), the five most recently used programs (by the active User) are
listed. These programs can be started directly from the block menu (select with cursor and push
[start] Button).

5.5 System Menu

To enter the System Menu, press the System Menue Button above the display.

The System Menu allows global settings and provides information about the instrument. The
power on self test log files can be retrieved and service files generated, a detailed description of
this menu is shown in section 13, page 41.

5.6 Program Menu

To enter the Program Menu, press the Program Button above the Display.
Service Manual TProfessional ______________________________________________________ 13

In the Program Menu, existing programs can be edited or new programs can be created. Each
User has his own program directory, where individual programs are saved. Programs from other
user directories can be used, but not modified.

5.7 Navigation within software menus

Four navigation keys provide easy navigation within the software screens. The [left] and the
[right] key have additional functionalities as described below:

The right cursor key moves the cursor to the next field.

This cursor can also be used to confirm data entry. By pressing the right cursor
settings will be saved and the cursor moves to the next field.

In the file directory, this key moves to cursor forward to the next (lower) level.

The left cursor key moves the cursor back to the previous field.

In most screens this cursor is equivalent to the ”back” key.

In the file directory, this key moves the cursor back to the higher level.

6 Login In
The TProfessional manages up to 30 individual users. For each user a directory is created, where
programs are stored. Programs from other directories (i.e. users) can be read but not modified.
This allows to use all programs that are in the cycler memory, but to write only in the own user
directory.

Each User is identified by User Name plus Initials (2-3 letters).

Each User account can be protected by a PIN consisting of 1-7 digits. If no PIN protection is
wanted, leave the field “Enter PIN” empty and confirm with [Enter].
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Note: If no PIN protection is used, programs in the User directory may be changed or deleted by
unauthorized persons.

6.1 Log in existing User

Press Menu Button [Log in/out] above the Display. A list of Users is shown

Select User with cursor keys, or enter first initial. The cursor jumps to the first account starting
with this letter.

Press Soft Key [log in] and enter your PIN.

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Confirm PIN with button [PIN code ok]. The current User is shown:

To proceed with editing or running a program, select one of the Menu Buttons above the display

6.2 Create new user account

Press Menu Button[Log in/out] above the Display.

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To create a new User account, press Soft Key [New User].

Enter your name and 2-3 initials. Enter a personal identification number (PIN) consisting of 1-7
digits. For secutity reasons, you are requested to repeat the entry of your PIN.

Note: PIN entry is optional. If no password protection is wanted, just confirm with [User data
OK]. However, if no PIN is entered, you account containing all of your programs can be
modified by unauthorized personal.

Note: Your PIN can be changed any time in the System menu (see section 0, page 42).

6.3 Supervisor Log in

Only the Supervisor is authorized to change global instrument settings. This includes update of
firmware and the deletion of user accounts.

To log in as Supervisor, press the Log in/out Menu Button above the Display. Press Soft Key
[Supervisor Log in] and enter Supervisor PIN.
Service Manual TProfessional ______________________________________________________ 17

The default Supervisor PIN is 000 000 0 (do not enter blanks).

6.4 Delete User

Once logged in, you can delete your own account in the System Menu (see section xxx).

In addition, any user can be deleted by the Supervisor. To delete an user, log in as Supervisor an
select option “delete user”.

Note: By deleting a User Account all programs in the referring user directory will be deleted. It
is therefore recommended to safe the programs in a different directory prior to deleting a User
account.
18 ___________________________________________________________________________

7 Create program
To create a new or edit an existing program, press the [Program] Menu Button above the Display
(see section 5.6).

To create a new program move cursor to your directory and press Soft Key [Open directory].

Press Soft Key [New Program].

7.1 Set Block Type

The TProfessional Thermocycler automatically recognizes the installed block module. This
module is the default block type for creating a new program.
Service Manual TProfessional ______________________________________________________ 19

However, it is also possible to write a program for an alternative block type (i.e. w/o gradient).
The block type can thus be set manually by pressing the Soft Key [block Type].

To create a program for a block type other than the one currently installed, please select one of
the listed block types.

7.2 Heated lid settings

7.2.1 Set lid pre-heating mode

You can choose whether the lid is pre-heated before the program starts. This is to avoid
evaporation during the initial heating phase.

Note: During the preheating of the lid, the block is held constant at 25°C.

Note: After reaching the set lid temperature, there is a 40 second equilibration phase before the
block starts.

Note: The default setting, which is recommended for most applications, is preheating: On.
20 ___________________________________________________________________________

However, if the program should start while the lid is heating, this can be set by pressing the Soft
Key [preheat on/off].

After Lid settings have been made, confirm with [Enter] to enter the programming spreadsheet.

7.2.2 Set lid temperature

Note: Thanks to the design of the new High Performance Smart Lid, lower lid temperatures can
be used for thermocycling than in the past. Thanks to the lower lid temperature a higher
temperature uniformity between samples can be achieved.

The recommended lid temperature is 99.0°C.

Enter temperature and confirm with [Enter] or step to the next field with cursor key.

7.3 Enter step temperature and time

Service Manual TProfessional ______________________________________________________ 21

Use cursor keys to navigate in the programming table.

Note: Each setting is confirmed with [Enter]. The cursor moves automatically to the next field.
Alternatively, you can confirm a value by moving forward with the cursor keys.

Enter temperature for the first step an press [Enter]. In the next row you can enter the time for
this temperature:

Note: Minutes and seconds can be separated by “dot”, but this is optional. You can also enter the
digits one after the other. Example: to set 2 minutes, 30 seconds enter “2”, “3”, “0”

7.4 Set loop

Note: In general, loops are entered in the LAST STEP of the loop. A loop is defined by
selecting the FIRST STEP in the loop plus the number of back loops.

Enter the number of the first step within the loop in the row “go to” and confirm with [Enter].

Enter the number of back loops in the row “loops”.

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The loop is shown as a bracket at the left side of the spreadsheet.

Note: Total cycler number = (number of back loops) + 1, e.g. enter 24 for a total cycle number
of 25.

7.5 Cool below ambient temperature

After the program has been finished, samples can be kept at below ambient temperature.

Note: To save lifetime of Peltier elements, a temperature of 15°C for the final step rather than
4°C is recommended.

7.6 Program Pause

To hold a temperature for an indefinite time enter “0”. After confirming with [Enter] the word
“pause” is shown.

7.7 Save program

Press button [Save / Save as].

Service Manual TProfessional ______________________________________________________ 23

The first available program number is automatically pre-selected. Use cursor keys (or enter two
digits) to move to a different program store.

To enter a program name press button [edit name].

Enter program name and confirm name with button [ok].

24 ___________________________________________________________________________

Confirm program store number with Soft Key [Save].

Program number, name and date are shown in the file directory.

To run this program press Menu Button [block] above the display.
Service Manual TProfessional ______________________________________________________ 25

Press the (illuminated) [Start] Button on the left side of the keypad.

8 Set Gradient
The easiest way to define a gradient is to enter the two temperatures of the block (left and right
side) separated by “-“.

Confirm with [Enter] or move cursor to the next field.

Alternatively, move cursor to the field for temperature setting and press Soft Key [Gradient].

In the Gradient Screen you can enter a new, or edit an existing Gradient. Enter new settings for
Row01 (left side) and Row12 (right side) and confirm with Enter.

To go back to the spreadsheet, press Soft Key [Table].

26 ___________________________________________________________________________

9 Edit programs
Programs can be edited in the Table or Graphical mode [Graph]. To change between these two
modes click on the Soft Key [Table] or [Graph] respectively. The graphical mode is intended for
easy modification of existing programs, which can then be saved under a new program number
and name. To edit programs in the graphical mode see section 9.5, page 31.

Note: During operation of the Thermocycler the active program can be viewed but not modified.
If you want to change settings of the active program for further experiments you have to save a
copy of this program to another memory. For further information about copying programs see
section 9.3, page 29.

9.1 Insert program step

Move cursor to the position, where a new step should be inserted

To insert a program step press Soft Key [Insert/Del]. The step at which a new step will be
inserted is highlighted.
Service Manual TProfessional ______________________________________________________ 27

Press Soft Key [insert step].

Confirm with Soft Key [Yes]. Enter temperature and time settings for new step.

Note: The new step has been entered within an existing loop, this loop now consists of four
steps.

9.2 Delete program step

Move cursor to the step that should be deleted and press Soft Key [insert/delete step].
28 ___________________________________________________________________________

To delete the highlighted step press Soft Key [Insert/Del]

Confirm deletion of step number 4 with Soft Key [YES]

Service Manual TProfessional ______________________________________________________ 29

The total step number is reduced by one, the loop now contains 3 steps only.

9.3 Copy program

Press Menu Button [Program] and enter User directory.

Move cursor to the program you want to copy.

Press Soft Key [Copy Program]. The cursor automatically jumps to the next available (free)
program store.

The program copy can be stored at any storage number. To change program number move the
cursor to the wanted location or directly enter a storage number (two digits) over the keypad.

Edit name and confirm with Soft Key [Save Copy]

30 ___________________________________________________________________________

9.4 Delete program

Select a program from your user directory

Press Soft Key [Delete program].

Confirm Deletion with Soft Key [Yes].

Service Manual TProfessional ______________________________________________________ 31

This program store is now free.

9.5 Editing programs in the graphical mode

Programs are created in the spread sheet mode where new steps are easily defined by
Temperature and Time. In addition, programs can be edited in the graphical mode which
provides a schematic temperature plot of the different steps.

To enter this mode, press Soft Key [Graph].

Spread Sheet Mode [Table] Graphical Mode [Graph]

To change temperature and time settings, move cursor to the respective field and enter new
values with the key pad. Once confirmed with enter, the temperature graph is updated.

Note: The steps are automatically spread over the whole range to give maximum resolution (thus
steps height does not represent the real temperature).

Note: To set loops, go to the Spread Sheet Mode (press [Table])

Note: To insert or delete program steps, go to the Spread Sheet Mode (press [Table])
32 ___________________________________________________________________________

Note: To edit or enter gradients, move cursor to the respective step and press Soft Key
[Gradient]. Enter gradient temperatures as described in section 8, page 25).
Service Manual TProfessional ______________________________________________________ 33

10 Change program options

For each step additional options can be defined.

To change program options, move the cursor to the right side of the spreadsheet, labelled with
“Options”. A new table is displayed.

To go back to programming sheet, move the cursor to th left side of the display

If options have been changed from the default values, there is an indication in the options field.

10.1 Heating and cooling rate

Since the TProfessional is a very fast thermocycler it may be necessary to reduce the heating and
cooling ramp to adopt protocols from slower thermocyclers.

The heating or cooling ramp respectively can be set in the row labelled with Ê[°C/s].
34 ___________________________________________________________________________

Note: This entry specifies the speed at which the referring step is achieved. I.e. to heat from step
1 to step 2, the heating rate in step 2 has to be lowered.

Note: To reduce the over all speed of the Thermocycler, the heating rate has to be modified in all
steps. The speed settings will be only valid for this individual program.

10.2 Time increment Æ Δt(s)

To compensate for loss in enzyme activity, each step within a loop can be extended from cycle to
cycle. Enter the desired time increment [seconds] in the row labelled with dt[s]. This value will
be added to the time value from cycle to cycle.

Note: A time increment will increase the total runtime depending on the numbers of cycles and
the size of the increment. A program with many cycles and large time increments will take a
significantly longer time than a standard protocol.

10.3 Temperature increment or decrement Æ ΔT(°C)

For some applications it is useful to start with a higher temperature and to decrease this
temperature from cycle to cycle. This subsequent lowering of a temperature is called touch
down.
Service Manual TProfessional ______________________________________________________ 35

To decrease a temperature from cycle to cycle enter a negative temperature increment in the row
labelled with ΔT[°C].

Note: Be sure that the temperature decrease is set in a step that lies within a loop. Otherwise
there will be no iterative temperature decrease.
36 ___________________________________________________________________________

11 Run program
To start program press [Start] Button (see section 4.4, page 7).

This button is only active, when a program for start has been selected. This is indicated by the
green LED in the Start button.

11.1 Select program for start from User directories

Any program in the cycler memory can be used to start the thermocycler. In addition to the
programs in the own User account, also programs from other User directories can be started.

To select a program for start press [Program] Menu Button above the Display.

Select directory and program with cursor keys and press [Start] Button.

11.2 Quick start programs from the block Menu

The TProfessional provides the last 5 programs that haven been run for quick start.

Push the [block] Menu Button above the Display.

Service Manual TProfessional ______________________________________________________ 37

Select one of the last five programs with the cursor keys.

Press the (illuminated) Start button on the left side of the keypad (see section 4.4, page 7).

11.2.1 View program prior to start

To check a program prior to start press the [View Program] Soft Key.

The current program is shown.

Press [View Graph] Soft Key for alternative display.

38 ___________________________________________________________________________

View Table View Graph

Note: In the view program mode, a program cannot be edited. To edit program press [Program]
Menu Button above the Display.

11.3 Display during operation

After the instrument has been started, the below display is shown

To show detailed information about the running program, press one of the Soft Keys [Show
Graph] and [Show Table]..

There are three different display modes available during the run.
Service Manual TProfessional ______________________________________________________ 39

Show Time Show Graph Show Table

11.4 Pause Program during run

To pause program press Soft Key [Pause Block].

The message “PAUSE” is shown in the display and alternates with the elapsed time.

11.5 Continue program

To continue a paused program press Soft Key [Continue]

40 ___________________________________________________________________________

12 Stop program
To stop the active program press [stop] button.

To abort the active program confirm with [Yes].

12.1 Stop Program from a pause

If the last step in a program is a pause, the program has to be stopped by pressing the [Stop]
Button before switching off the instrument.

Note: If the instrument is switched off during a pause step, this will be recognized as a power
failure (a referring error message is written to the log file).
Service Manual TProfessional ______________________________________________________ 41

13 System settings
To change global settings press the [System] Menu Button above the screen.

Main screen

13.1 User configuration

This option allows changes of your individual user account.

13.1.1 Set language

The language settings are part of each User account. Available languages are English and
German (second software release)
42 ___________________________________________________________________________

13.1.2 Change individual PIN

Thi option allows changing the PIN of the current User.

13.1.3 Delete Account

Any user can delete his or her own account.

Important: Deleting an User account will automatically delete the user file directory together
with all programs stored in this directory.

Proor to deleting an account, ensure that the User directory is empty, or save a copy of the
programs for further use to a different User directory (see section 9.3).

13.2 System configuration

This option allows setting of the signal tone (beep) , Display contrast and time settings.
Service Manual TProfessional ______________________________________________________ 43

13.2.1 Configure beep

To switch beep on/off toggle between On and OFF and confirm with Soft Key [Set Beep].

13.2.2 Configure Contrast

The Display contrast can be set individually for each User.

Change settings with Cursor keys and confirm with Soft Key [Save Contrast].
44 ___________________________________________________________________________

13.2.3 Set time and date

Enter settings for Date and Time and confirm with Soft Key [Set Time and Date].

13.3 System info

This Menu provides information on the instrument and on the Log files of the last five runs.

13.3.1 System info

Use this option to Display the instruments serial number, block type and software version.
Service Manual TProfessional ______________________________________________________ 45

13.3.2 View Log files of the last five runs

During each run a log file is stored in the thermocycler memory. The log files of the last five
runs can be viewed in this window.
46 ___________________________________________________________________________

13.4 Service

13.4.1 View log files of Power on self test

During boot up (Power on) the TProfessional hard ware is checked (Self test). Here you can see
the results of the last five self tests.

13.4.2 Execute extended self test

In addition to the regular Power on –Self test, a more comprehensive test can be triggered by the
user. This test takes several minutes. During the test no programs can be run on the
thermocycler.

It is recommended to execute an extended self test regularly. The results of the extended test can
be viewed in the following menu:
Service Manual TProfessional ______________________________________________________ 47

13.4.3 View log files from extended self test

Display results of the extended self test.

13.4.4 View Error log files

Histories of all software and hardware errors are shown in this menu.

Note: Sever errors that require action by the User are displayed in the User Software Menu.

13.4.5 Create Info file for Biometra Service Department

This option creates a Service file containing technical details for the Biometra Service
Department.

13.5 How to contact Biometra

48 ___________________________________________________________________________

14 Maintenance

14.1 Cleaning and Decontamination

The TProfessional was built to operate for a long time without the need for periodical
maintenance. The Thermocycler housing may be cleaned from time to time with a smooth cotton
cloth. Do not use strong detergents or organic solvents for cleaning. Never treat silver block with
abrasive agents.

Important: Appropriate safety regulations must be observed when working with infectious or
pathogenic material.

14.2 Trouble shooting

14.2.1 Slow heating and cooling

The TProfessional is equipped with a strong ventilator for the cooling of the heat sink. The inlet
of this fan is located at the bottom side of the instrument. Be sure that the inlet is not clogged by
dust or other material (e.g. a sheet of paper placed under the cycler can be attached to the inlet as
the fan is in operation). Dust can be removed easily from the inlet with a conventional vacuum
cleaner.

14.2.2 Restart due to unrecognised power failure

High voltage fluctuation can lead to an automatic restart of the thermocycler. In this case the
cycler restarts at the step where there power failure has occurred. To avoid voltage fluctuation,
do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a
centrifuge.

14.2.3 Adaptation of protocols from other cyclers

Since the TProfessional is a fast instrument it may be necessary to reduce the heating and
cooling ramps to run protocols from other cyclers. For the setting of the heating and cooling
ramps see section 10.1. Alternatively, the time settings may be extended.

14.2.4 Releasing wheel in case of blocked lid

When the lid is in the extreme up or down position, it may happen that the wheel is uncoupled.
In this situation the clutch mechanism is active in both directions (clicking noise in either
direction).

To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism (see section 4.6, page 8). Thus, care must be taken not
to apply excessive pressure.

Important: When the clutch mechanism is active (= optimum pressure is applied). Do not
use pin to further increase lid pressure. This will lead to damage of tubes and instrument!
Service Manual TProfessional ______________________________________________________ 49

15 Service and repair

The TProfessional Thermocycler contains no user serviceable parts. Do not open the housing
instrument. Service and repair may only be carried out by the Biometra Service department or
otherwise qualified technical personal.

15.1 Spare parts

Please see the current spare parts list of the TProfessional you will receive from Biometra’s
service centre. E-mail: info@biometra.com

15.2 Assembling instructions

Safety!
Read this section carefully before performing any maintenance operation on the TProfessional
Thermocycler.

Service personnel must restrict their maintenance operations to those described in this service manual.

In the interest of safety, it is essential that service personnel follow generally accepted laboratory
procedures and pay particular attention to the safety notices specified in this service manual.

Voltages present inside the instrument are potentially dangerous.

If work has to be carried out with the instrument housing removed, prior turn
off the main switch and disconnect the power cord.
Any voltage measurements must only be taken by personnel qualified to read and follow electric circuit
diagrams.

Important information
This assembling instruction deals with the maintenance and repair of the TProfessional Thermocycler
including component replacement. Servicing technician must restrict their servicing activities to the
identification and exchange of standard components.

All exchanged components must be sent to Biometra biomedizinische Analytik GmbH for repair or
exchange.

Service technician must restrict their reparation and replacement tasks to those described in this service
manual.
50 ___________________________________________________________________________

15.2.1 Main groups

15.2.1.1 Air baffle (9-020-010)

Figure 1 Assembling the air baffle (Fig. 1):
Unlock and remove the block before
detaching the air baffle. Unscrew the two
screws (M3x6). Disconnect both lock
washers. Carefully remove the baffle in front
direction.

15.2.1.2 Fan chassis (9-020-011)

Figure 2 Disassembling the air baffle (Fig. 2):
For inspection or cleaning procedure place the
instrument on the side and remove all four
screws (M3x6) near the fan grid on the ground
plate.
Lift up the fan chassis about 5 mm and remove
it from the slot by gently moving in front
direction. Finally disconnect the small plug of
fan wire from the back side of the processor
board.
Service Manual TProfessional ______________________________________________________ 51

15.2.1.3 Back cover plate (9-020-012)

Figure 3 Assembling and dissembling of
back cover plate (Fig. 3 to 5):
Remove the four hexagon screws
(M3x6) fixing the back cover plate
to chassis and remove the two
hexagon screws (M2, 2) from the
sub-D socket.
For assembling of back cover plate
handle in the opposite direction

Figure 4 Figure 5 Disconnect the cables (Fig. 4 + 5):

Detach the back cover plate.
Disconnect the plugs of yellow and
brown cables from mains filter
(Fig. 4).
Unplug the earth conductor which
is placed on ground chassis left
hand near display-I/O-board
(Fig. 5).
52 ___________________________________________________________________________

15.2.1.4 Housing complete (9-014-001)

Figure 6 Disassembling the housing (Fig. 6 + 7):

Use a soft pad for protection of scratches.
Turn the device carefully upside down.
Remove the 6 screws on the ground plate of
the chassis (Fig. 6). Turn around the device
and remove the two screws fixing the
housing and the back plate (Fig. 7). Finally
lift off the housing.

Figure 7

Assembling of the housing (Fig. 6 + 7):

Place the housing carefully onto the ground
chassis. Fix the housing and the back plate
with two screws on the rear side of the
device (Fig. 7). Protect the housing for any
scratches by using a softy pad. Carefully
turn upside down the device. Assemble the
housing and ground plate with six screws
(Fig. 6).

Figure 8 Disconnect the cables (Fig. 8):

Place the housing on a soft pad aside the
chassis and disconnect the cables going
from the keypad and display to the display-
I/O-board.
Connect the cable (Fig. 8):
Important: First of all check the correct
polarity of all cables before plug in the
display-I/O-board. Therefore each electrical
connection must show in outside direction.
Close the housing of device after display
test procedure (5 seconds). It must show a
screen layout.
If there is no menu display visible, check
up the correct orientation for the contact
section of display cables.
Service Manual TProfessional ______________________________________________________ 53

15.2.1.5 Fixed modules (Tprofessional STANDARD; TProfessional BASIC)

Before disassembling fixed modules of the TProfessional STANDARD or TProfessional
BASIC disassemble the housing (section 15.2.1.4, page 52) and the back cover plate (section
15.2.1.4, page 52). Afterwards disconnect the cables.
Figure 9 Disconnect the cables (Fig. 9):
Disconnect the D-sub plugs 37-pin cables
coming from the processor board.
Note the correct position of the heated lid and
block wires before disassembling.
Connect the cables (Fig. 9):
1 2 3 4 5 6 7 8 9 10 Connect the cables conform to the following
schema.

ATTENTION: Never use the cable end caps a second time!

The cable end caps will be delivered from Biometra Service Department with each
inspected module. Only use new crimped cable end caps and a torque wrench with a
torque of 30 Ncm to fix the heated lid and block wires in the terminal strip of the
module.

Finally connect the D-sub plugs 37-pin cables coming from the processor board.

position colour description position colour description

1 yellow heated lid - heater 5 green block 1 left section
2 yellow heated lid - heater 6 yellow block 1 left section
3 green heated lid - lid sensor 7 white block 2 middle section
4 white heated lid - lid sensor 8 blue block 2 middle section
9 black block 3 right section
10 red block 3 right section
54 ___________________________________________________________________________

Figure 10 Disassembling of the fixed module

(Fig. 10-12):
Screw off both upper and lower cross head
screws fixing the module to the chassis.
Unscrew the M3 screws which fixing the
Block on both sides of chassis.

Figure 11 (right sight module fixation) Figure 12 (left sight module fixation)

Figure 13 Take the module out of the chassis

(Fig. 13):
Press the left and right side panel of the
chassis in outside direction (1).
Lift the module when the two lateral notches
2 2 of the heat sink release the two bolts placed
1 right- and left-hand of the bear rails (2).
1

Assembling of the fixed module:

ATTENTION: Before fixing the module and the chassis make sure that the module is places
in a centred position between both vertical side panels of the chassis. Otherwise the housing
cannot be assembled on the chassis correctly.
Service Manual TProfessional ______________________________________________________ 55

15.2.2 Housing

15.2.2.1 Housing and display (7-014-071; 9-073-022)

Figure 14 Disassembling of the display (Fig. 14):
Remove the two screws (2,2 x 6,5) fixing
the display. Pull out the display board to
free direction of block until both black clips
move out of the catches (Fig. 14).

Figure 15 Assembling of the display (Fig. 14 + 15):

Check up the correct position of rubber
foam on the frame of display (Fig. 10),
before assembly. Remove the protection foil
from the display. Push the black clips
carefully into the catches (Fig. 9). Fix the
display with two screws (2,2 x 6,5) on the
inner side of housing.

Figure 16 Figure 17 Test of the background

illumination (Fig.16 + 17):
Before assembling of housing check
up the proper function of display
background illumination.
Use an external power supply at 5,0
volt DC. Connect the small display
plug with right polarity.
Red cable plus (+)
White cable minus (-)
56 ___________________________________________________________________________

15.2.2.2 Keypad foil (7-076-019)

Figure 18 Remove / exchange the keypad (Fig. 18):

If present detach the ferrite core from the
rear side for housing before remove of
defective keypad. Unplug the keypad and
clean the keypad area of the housing from
adhesive residues.
Important: Handle with care because the
ferrite core has to be reinstalled on the
same position.
Assembling of keypad (Fig. 18):
All keypads have a removable special
protection foil on both sides.
Start to install the keypad cable through the
small slot on panel area. Detach the
adhesive protection foil from backside.
Fit the keypad on the bottom edge of the housings. Make sure a balanced position in centre
of keypad area. Apply the keypad carefully. Pull the ferrite core over the cable and fix it with
silicon on the inner side of housing. Remove the protection foil on the front side of the
keypad after process.

15.2.3 Heated lid

15.2.3.1 Heated lid seal (7-014-081)

Figure 19 Exchanging of the heated lid sealing

(Fig. 19):
Open the lid. Remove the six screws
(M3x6) they fix the housing on the lid
bottom plate. Remove the old lid sealing
carefully.
Insert the new sealing. Thereby turn gently
the six screws only two turns and keep a
small slot. Fit the new sealing into this
small slot. Push it between bottom plate and
housing shaft. After fitting of new sealing in
a straight line and correct position, seize all
six screws.
Service Manual TProfessional ______________________________________________________ 57

15.2.3.2 Connection of heated lid

Figure 20 Disassembling of the heated lid
connection (Fig. 20):
For disassembling of the housing lid
remove the plug from heated lid. Cut
and remove the old cable clips. They
locate the cable in optimize and save
position. Disconnect the plug as (s.
figure).
Assembling of heated lid plug
(Fig. 20):
housing of lid plug
Fix the lid cable with a small loop on
right hand. It allows a free swing of
the cable while moving of the heated
lid. Test the isolation of cable for any
damages. Fix the new lid plug to the
position on left axle support (Fig. 15).

15.2.3.3 Lid plug

Figure 21 Disassembling of the lid plug (Fig. 21):
After disassembling cut the blue and brown
wires near the plug housing. They are
unemployable afterwards.
Assembling of the lid plug (Fig. 21):
The spare part kit of the new heating element
contains four crimp connectors and one new
plug housing. Fix the cable ends into the
connectors. Push all pins into the plug
housing and check up the correct polarity.
The cables differ in their Ohm resistance:
2x sensor = blue wires = 10 Kilo Ohm
2x heater = brown / grey wires = 10 Ohm
58 ___________________________________________________________________________

15.2.3.4 Knob (9-010-033)

Figure 22 Disassembling of the knob (Fig. 22):
Unscrew the two small black cross head screws on
top of knob. Detach the cover plate and the arresting
pin with its spring. Remove the locking washer from
the top of the centre bold. Finally remove the
complete knob.
Assembling of the knob (Fig. 22):
Check the intactness of centre bolt before assembling
the new knob. Attach the knob with the ball bearing
gently onto the centre bolt. Fix the knob with the
locking washer on the axis. Insert the arresting pin
with the spring into the pins hole. Attach the cover
plate on the knob. Finally inspect the function of the
components of the knob.

15.2.3.5 Planetary gear (9-010-034)

Figure 23 Disassembling of planetary gear (Fig. 23):
Before exchanging or repairing the planet gear
remove the knob. Detach the sun gear, the four
planet gears and washer. Unscrew the two flat head
screws to remove the complete heating element from
the housing of the lid.
Assembly of the planetary gear (Fig. 23):
Place the heating element so, that the threaded bolts
look through the four holes of the planetary gear
area of the lid housing. Screw the four gear wheels
with four PVC washers onto the four threaded bolts.
Do not fix them too close otherwise the heating
element can not be adjusted. Make sure an even
position of the heating element before fixing the sun
wheel with its washer 36 x 10 x 0, 25 PA
(Fig. 18).Lubricate carefully all four threaded bolts
and gear wheels with a little grease (Topas; NCA
5051). Finally fix both flat head screws onto the
spring plate.
Service Manual TProfessional ______________________________________________________ 59

15.2.3.6 Fixing of heating element;

TProfessional 60 (9-010-007), 96 (9-010-006) and 384 (9-010-008)
Figure 24 Disassemble the heating element (Fig. 24):
After dismounting the planet gear and flat head
screws pull the lid cable through the heated lid frame
and remove the heating element carefully.
Assemble the heating element (Fig. 24):
Push the cable from heating element through the hole
in the lid frame and assemble the heated lid as
described before.

15.2.3.7 Spring plate (9-010-035)

Figure 25 Disassembling of spring plate (Fig. 25):
Remove the two flat head screws (6) from
the spring plate.

1 6
Assembling of spring plate (Fig. 25):
1 Carefully, add four springs on spring plate
and fix them with two flat head screws (1).

4Figure 26 Description of spring plate (Fig. 26):

Four flat head screws (M6x30) are fixed on
5 the spring plate (2). The screw heads have to
2 show in the direction to the countersinks (3)
for the four springs, arranged on the back
side of heating element (Fig. 27; (4)).

5
3

Figure 27 Description of Heating element (Fig. 27):

While assembling please use a softy pad
which is protecting the heating element
surface for any scratches. Place the four
springs into the countersinks on the backside
4 4 of the heating element and attach the
preassembled spring plate.
60 ___________________________________________________________________________

15.2.4 Chassis
ATTENTION: All power and processor boards should be replaced together.
Only pair wise matched controller and processor boards obtained from BIOMETRA
Service Department ensure a technically faultless functionality of the TProfessional
thermocyclers, within the specified technical specifications.

15.2.4.1 Power board (9-070-013)

Figure 28 Test the condition of the power
board before assembling (Fig. 28):
Inspect the condition of fuse holders:
They must cover the end caps of 16
Ampere fuses very strong.
s. Fig. 24 Make sure that both ends are fixed by
solder joints. They do not have to
1 become dark.
Otherwise it is advisable to exchange
the holders together with new fuses.
Finally inspect the condition and
position of the protection strip (1) on
metal side plate of chassis.
Figure 29 Wiring of the power board (Fig. 29):
Fix the different colored peltier cables
with 35 Ncm in the socket on the back
side of the power board. Figure 29
gives an overview with the correct
position and color of Peltier cable:
Red = Block no. 1
Black = Block no. 1
Blue = Block no. 2
White = Block no. 2
Yellow= Block no. 3
Green = Block no. 3

Figure 30 Turn up the mounting side of power

board for correct assembling on
chassis (Fig. 30).
Service Manual TProfessional ______________________________________________________ 61

Figure 31 Figure 31: Insert the power board in the

free area of the chassis from the left hand
side.
Note: All wires have to be positioned in a
relaxed and big loop under the power
board.

Figure 32 Figure 32: Fix the power board on

the four spacers with cross head screws
(M3x10).

Figure 33 Connection of power board (Fig. 33):

Connect the wires (crimped with cable

end caps) coming from the secondary
Brown(1)

White(3)
Black(4)

side of transformer to the green print

Blue(6)

socket which is placed on the fore side

angel of the power board. Fix all
screws with a torque of 50 Ncm.
Start up counting the position of screws
from left to right. Install as following
cable to socket position:

Brown = Position no. 1

White = Position no. 3
Black = Position no. 4
Blue = Position no. 6
Free = Position no. 2 and 5
Figure 34 Before the installation of power board
make sure to place the cable in position
behind the spacer to protect for any
contacts to metal chassis (Fig. 34).
62 ___________________________________________________________________________

Control and protection components of the power board (Fig. 35):

All high sensitive components of the power board are fuse-protected.
There are two version of fuses placed on the power board:

2x 16 AT fuses (no.: 8-501-048) slow blow-solder version to safeguard the main circuit and
3x 8 AT fuses (no.: 8-501-013) slow blow to safeguard the Peltier circuit.
Figure 35

8-501-048

8-501-013
Service Manual TProfessional ______________________________________________________ 63

15.2.4.2 Processor board (9-070-014)

Figure 36 Processor board assembling (Fig. 36):

The processor board is placed left hand
1 2 of chassis. It is fixed by four cross head
screws (M3x10). On the left upper
position isolate the screw towards the
power board with an isolation washer
(1). On the right upper position ground
the power board by using a lock washer
behind the screw (2).
Before fixing the processor board move
the board to its correct position. Prevent
a clamping of the small socket of fan
plug which is fixed on the backside of
processor board. Centre the socket to its
chassis opening on the left chassis wall.
Finally screw the processor board tight.
Figure 37 Processor board wiring (Fig. 37/38):
D-sub plugs 37-pin, (1a) the 10-pin, (1b)
split from the 37-pin cable and the D-sub
plug of the 50-pin cable from the power
1b board (2)
D-sub plug of the 10-pin cable from the
display-I/O-board (3)
They have to be connected with the
1a
3 processor board as showed in figure 37
2 and 38.

Figure 38 Particularly the 50 pin flat ribbon cable

(Fig.38,(4)) must be placed very close to
the backside of 37 pin plug housing to
protect the cable against any damage
during the closing of the device housing.

3
4
64 ___________________________________________________________________________

Control and protection components of the processor board (Fig. 39):

The processor board features the following control and protection components:

Processor Part no.: 8-503-024

Eprom Part no.: 9-503-013
RAM Part no.: 8-503-085
2x fuses 1 AT slow bow Part no.: 8-501-003
Figure 39 8-503-024

8-503-085
9-503-013

8-501-003
Service Manual TProfessional ______________________________________________________ 65

15.2.4.3 Display I/O board (9-070-012)

Figure 40 Display-I/O-board assembling (Fig. 40):

The display-I/O-board is placed right hand
1 on chassis and fixed by four cross head
screws (M3x10).
2 The display-I/O-board is grounded on the
left upper position (1) by a lock washer
behind the screw.
Before assembling the display-I/O-board
onto the chassis move the board to its
correct position. Fix the RS 232 interface
plug of the display-I/O-board (2) with the
backside of the back cover plat.
Figure 41 Display-I/O-board wiring (Fig. 41):
The display-I/O-board has got one electric
3 power point (3) for the display illumination
Beside them there are three data links for
4 the connect of display-I/O-board to:
display (4)
5 key pad (5)
processor board (6)
6

Figure 42 Attention! (Fig. 42):

Near the chassis wall the 10-pin flat ribbon
cable with its D-sub plug has to be fixed
with a cable clip (1). Fix it together four
different coloured cables. Coming from the
transformer and going to the power board.
In order to avoid a contact of the 10-pin flat
1 ribbon cable with the single isolated line
segment of the 110 to 220 volt primary
circuit in the region of the voltage sector
switch (2).
2 Place the end of the 10-pin flat ribbon cable
in the region where the primary circuit
cables extra protected with a fibre glass
hose.
66 ___________________________________________________________________________

15.2.5 Back cover plate

Figure 43 describes the wiring of the system line filter and the power switch:

Blue wire (3) = neutral

Brown wire (4) = phase
The yellow/ green earth cable (5) = earth

Figure 43

blue (neutral)
3

green/yellow (earth) 5
4
b ( h )

The power switch is fixed by two screws M3x6 outside on the back cover plate. The system
line filter is positioned on the two thread gear bolts of the back cover plate and fixed with
two lock washer and two M3 nuts. The earth cable (yellow/green) connects with its short end
the earth contact from the system line filter with the earth contact of the power switch. The
long line segment of the earth cable (yellow/green) has to be connected to chassis, left hand
under the processor board.
Please note: Make sure that all outgoing cables from the power switch don’t stick up higher
than the system line filter.
Service Manual TProfessional ______________________________________________________ 67

15.3 Wiring diagram

68 ___________________________________________________________________________

15.3.1 Main Board description

ST1 Connector Power Board – Processor Board
STD Connector Transformer
STHD1 Connector Heated Lid Block 1 (2xHL Sensor, 2x Heating Element)
STHD2 Connector Heated Lid (n.c.)

SI1 Fuse Line Cord: 12 AT (soldered)

SI2 Fuse Line Cord: 12 AT (soldered)
SI150 Fuse Control Circuit 1: 8 AT
SI250 Fuse Control Circuit 2: 8 AT
SI350 Fuse Control Circuit 3: 8 AT

STP1 Connector Peltier elements Control Circuit 1

STP2 Connector Peltier elements Control Circuit 2
STP3 Connector Peltier elements Control Circuit 3

15.3.2 Processor Board description

ST1 Connector Processor Board-Main Board
ST3 Connector Control Circuit 1 to 3
STSER Connector Processor Board-Display I/O Board
STVENT Connector Fans

S1 Fuse Line Cord: 1 AT

S2 Fuse Line Cord: 1 AT

RT160 Potentiometer Current

RT100 Potentiometer 95°C
RT101 Potentiometer 0°C
RT260 Potentiometer Current
RT200 Potentiometer 95°C
RT201 Potentiometer 0°C
RT360 Potentiometer Current
RT300 Potentiometer 95°C
RT301 Potentiometer 0°C

IC67 RAM
IC66 Eprom
IC60 CPU

15.3.3 Display I/O Board discription

I1 Connector Display
I2 Illumination Display
I3 Connector Processor Board-Display I/O Board
I6 Connector keypad
Service Manual TProfessional ______________________________________________________ 69

15.4 Search Fault Trees

15.4.1 Fault tree “Bolck”

Error message: “No Block”

Start

"No Block"

no Switch off the device

Block module connected? Connect the block module
Switch on device

yes

no
Block module is fixed? Press locking lever

yes

no
Contact strip o.k.?

yes

no
Cable and PIN-connector o.k.?

yes

Return to Biometra Service

End
70 ___________________________________________________________________________

15.4.2 Fault tree “Display”

After switching-on the thermocycler

the display is still dark, no graphical Start
user interface is visible.

Display dark

no
Power cord connected? Check power cord

yes

no
Fuses on the PoBo o.k.? Solder in fuses
(2x 16 AT solderable)

yes

no
Fuses on ProcBo o.k.?
Replace fuses
(2x 1AT)

yes

no
Display illumination o.k.? Check display cables
Check transformer connections

yes

Return to Biometra Service

End

15.5 Firmware update

For instruction for firmware upgrade, please contact the Biometra Service Department.
Service Manual TProfessional ______________________________________________________ 71

15.6 Service repair form

To Biometra GmbH Service Department

Rudolf Wissell Str. 14-16, D-37079 Göttingen
Telephone (Direct Dial) +49(0) 551 50686-10/ 12
Fax Reply (Direct) +49(0) 551 50686-11
Service repair form (failure description)
Return Authorization Number *

Type of instrument /
order number *
Serial number *

Software version

Date of purchase and Biometra

RE number (invoice number) *

Description of problem *

International forwarding agent

Delivery procedure *
Standard procedure: The repaired product will be directly sent back after repair at
your cost if not specified differently (see below).
In case of a warranty repair Biometra will cover the freight charges.

In case you prefer the item to be included in the next regular ‰ with next
shipment please mark the field for this method. regular
delivery

Phone number
Fax number

Service person

Service person in charge or person to be contacted *

* Essential information. Without this information the repair procedure cannot start!
72 ___________________________________________________________________________

16 Instructions for return shipment

In case of an instrument failure that cannot be fixed by the procedures described in section 14.2
please proceed as follows:

• Return only defective devices. For technical problems which are not definitively recognisable
as device faults please contact the Technical Service Department at ( Tel.:
+49/551/5088-110/ -112, Fax: ++49/551/5088-111, e-mail:info@biometra.com).
• Important: Carefully clean all parts of the instrument of biologically dangerous, chemical or
radioactive contaminants. If an instrument is contaminated, will be forced to refuse
to accept the device. The sender of the repair order will be held liable for possible losses
resulting from insufficient decontamination of the device.
• Please prepare written confirmation that the device is free from biologically dangerous and
radioactive contaminants. The declaration of decontamination (see section 17) must be
attached to the outside of the packaging.
• Use the original packing material. If not available, contact Biometra or your local distributor.
• Label the outside of the box with “CAUTION! SENSITIVE ELECTRONIC
INSTRUMENT!”
• Please enclose a note which contains the following:
a) Sender’s name and address,
b) Name of a contact person for further inquiries with telephone number,
c) Description of the fault, which also reveals during which procedures the fault occurred, if
possible
Service Manual TProfessional ______________________________________________________ 73

17 Equipment Decontamination Certificate

To comply with German legislation (i.e. §28 StrlSchV, §17 GefStoffV and §19 ChemG) and to
avoid exposure to hazardous materials during handling and repair, please complete this form,
prior to shipping the equipment.

COMPANY / INSTITUTE
ADDRESS
TEL NO
FAX NO
E-MAIL

Instrument Model Serial No

For demo instruments Start Date End Date

Typ of hazardous materials used with this equipment:

Has the equipment been cleaned and decontaminated? YES NO

(please mark)

Describe method of cleaning / decontamination:

Name Position

(Head of Div./ Dep./ Institute/ Company)

Signed Date

PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA

DISTRIBUTOR TOGETHER WITH THE EQUIPMENT.
PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS
WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER.
74 ___________________________________________________________________________

18 Technical specification

High Speed High Speed Large graphical Display

Large graphical Display Large graphical Display Easy Spreadsheet
Features Exchangeable Blocks programming
Gradient version available Gradient version available Gradient version available
Blocks exchangeable Yes - -
60 well 60 well
Block formats 96 well 96 well 96 well
384 well 384 well
Program memory 350 average programs 350 average programs 350 average programs
Temperature range 3 – 99°C 3 – 99°C 3 – 99°C
Temperature gradient* 40°C 40°C 25°C
Cool samples to 4 °C Yes Yes Yes
Maximum heating 5.0°C/sec 5.0°C/sec 3°C/sec
Maximum cooling 3.5°C/sec 3.5°C/sec 2°C/sec
+/- 0.15°C at 55°C +/- 0.15°C at 55°C +/- 0.20°C at 55°C
Temperature Uniformity
(15 sec after clock starts) +/- 0.25°C at 70°C +/- 0.25°C at 70°C +/- 0.30°C at 70°C
+/- 0.50°C at 95°C +/- 0.50°C at 95°C +/- 0.60°C at 95°C
Control accuracy ± 0.1°C ± 0.1°C ± 0.1°C
Display ¼ VGA screen, ¼ VGA screen, ¼ VGA screen,
320 x 240 pixel 320 x 240 pixel 320 x 240 pixel
Auto restart after power Yes Yes Yes
Easy Spreadsheet Easy Spreadsheet Easy Spreadsheet
Programming mode Programming (ESP) Programming (ESP) Programming (ESP)
Graphical programming Graphical programming
Toggle between spreadsheet Toggle between spreadsheet
and graphical mode, and graphical mode,
gradient temperature graph*, gradient temperature graph*, Gradient temperature graph*,
Software options adjustable ramp rates,
adjustable ramp rates, adjustable ramp rates,
extended self test, service extended self test, service extended self test, service
files, automatic block files, PC control files, PC control
recognition, PC control
Quick start of the last 5 Yes Yes Yes
High Precision Smart Lid Yes Yes Yes
Lid temperature range 30 – 99°C 30 – 99°C 30 – 99°C
Max. power consumption 480 Watt 480 Watt 480 Watt
Noise emission Very low Very low Very low
Interface RS232 serial port RS232 serial port RS232 serial port
Power Supply 100, 115, 230 Volt, 50-60 Hz 100, 115, 230 Volt, 50-60 Hz 100, 115, 230 Volt, 50-60 Hz

Working conditions 15 °C to 35 °C, 70 % air 15 °C to 35 °C, 70 % air 15 °C to 35 °C, 70 % air

humidity, max 2000 m NN humidity, max 2000 m NN humidity, max 2000 m NN
Dimensions (W x D x H) 28 cm x 38 cm x 24 cm 28 cm x 38 cm x 24 cm 28 cm x 38 cm x 24 cm

* Applies to models with gradient feature

Service Manual TProfessional ______________________________________________________ 75

19 Warranty
This Biometra instrument has been carefully built, inspected and quality controlled before
dispatch. Hereby Biometra warrants that this instrument conforms to the specifications given in
this manual. This warranty covers defects in materials or workmanship for 24 months as
described under the following conditions:

This warranty is valid for 24 month from date of shipment to the customer from Biometra or an
authorised distributor. This warranty will not be extended to a third party without a written
agreement of Biometra.

This warranty covers only the instrument and all original accessories delivered with the
instrument. This warranty is valid only if the instrument is operated as described in the manual.

Biometra will repair or replace each part which is returned and found to be defective. This
warranty does not apply to wear from normal use, failure to follow operating instructions,
negligence or to parts altered or abused.
76 ___________________________________________________________________________

20 Declaration of Conformity

EU – Konformitätserklärung
EC - Declaration of Conformity
Göttingen, October 2007

im Sinne der EG-Richtlinie über elektrische Betriebsmittel zur Verwendung innerhalb

bestimmter Spannungsgrenzen 73/23/EWG
following the EC directive about electrical equipment for use within certain limits of voltage
73/23/EEC
und / and
im Sinne der EG-Richtlinie für die elektromagnetische Verträglichkeit 89/336/EWG.
following the EC directive about the electromagnetic compatibility 89/336/EEC .

Hiermit erklären wir, dass folgende Thermocycler:

Herewith we declare that the following Thermocyclers:

Typen / types: TProfessional Basic 96, TProfessional Basic Gradient 96

TProfessional Standard 60, TProfessional Standard Gradient 60,
TProfessional Standard 96, TProfessional Standard Gradient 96,
TProfessional Standard 384
TProfessional 60, TProfessional Gradient 60,
TProfessional 96, TProfessional Gradient 96,
TProfessional 384
Best.-Nr. / Order No.: 070-601, 070701,
070-850, 070-851, 070-950, 070-951, 070-952
070-800, 070-801, 070-900, 070-901, 070-902

den grundlegenden Anforderungen der

corresponds to the basic requirements of

EG-Niederspannungsrichtlinie 73/23/EWG und der

EC low voltage directive 73/23/EEC and the
EG-Richtlinie über die elektromagnetische Verträglichkeit 89/336/EWG entsprechen.
EC directive about the electromagnetic compatibility 89/336/EEC .

Folgende harmonisierte Normen wurden angewandt:

The following harmonized standards have been used:

EN61326 : 2004
EN 61010-1 : 2001 EN 61010-2-010 : 2003

Dr. Jürgen Otte

Formular 006, Ver. 03

Quality Manager
Service Manual TProfessional ______________________________________________________ 77

21 Note for the disposal of electric / electronical waste.

Note for disposal of electric / electronical waste

Hinweis für die Entsorgung von Elektroaltgeräten

du traitement des déchets des appareils

Renseignement électrique / électronique

This symbol (the crossed-out wheelie bin) means, that this product should be
brought to the return and / or separate systems available to end-users according
to yours country regulations, when this product has reached the end of its
lifetime.

For details, please contact your local distributor!

This symbol applies only to the countries within the EEA*.
EEA = European Economics Area, comprising all EU-members plus Norway, Iceland and Liechtenstein.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Dieses Symbol (die durchgestrichene Abfalltonne) bedeutet, dass dieses Produkt

von der Firma Biometra für eine kostenlose Entsorgung zurückgenommen wird.
Dies gilt nur für Geräte, die innerhalb Deutschlands gekauft worden sind.

Kontaktieren Sie für die Entsorgung bitte die Biometra Service-Abteilung!

Außerhalb Deutschlands wenden Sie sich bitte an den lokalen Händler.
Dieses Symbol gilt nur in Staaten des EWR*.
*EWR = Europäischer Wirtschaftsraum, umfasst die EU-Mitgliedsstaaten sowie Norwegen, Island und Liechtenstein.

--------------------------------------------------------------------------------------------------------------------------------------------------------------------------

Cet symbol (conteneur à déchets barré d´une croix) signifie que le produit, en fin
de vie, doit être retourné à un des systèmes de collecte mis à la disposition des
utilisateurs finaux en conséquence des régulations par la loi de votre pays.
Pour des information additionel nous Vous demandons de contacter votre
distributeur!
Cet symbole s´ápplique uniquement aux pays de l´EEE*.
EEE = Espace économique européen, qui regroupe les États membres de l´UE et la Norvège, Islande et le Liechtenstein.