GCSE Science Required Practical Questions
Q1 Based on taste a student suggests that the cells in one type of apple contain more sugar than the
cells of a different type of apple.
Don't think we're covered this
Describe and explain how a student can use a sugar solution of low concentration to test this
hypothesis. test to measure sugar
so I
just did a
Your account should include
a list of equipment and a description of how it would be used;
details of the measurements you would make;
details of how you would ensure that the experiment is fair;
the outcome you would expect if the hypothesis is correct.
of of and
•
Place one
piece each apple some size mass
into
a test tube .
•
Add some volume of Benedict 's solution to test tubes
•
Put them in a water bath of 80°C for 5 minutes
The colour of solution should change into either yellow or
green
•
, ,
orange
red in concentration if the colour the both
increasing
is same,
sugar ,
of If
would contain some concentration
sugar .
he is
right ,
two
colours would be different .
[8 marks]
4
GCSE Science Required Practical Questions
Q2 A student used the equipment shown in Figure 1 to model the effect of amylase on starch in the
gut.
beaker
visking tubing containing starch
solution and amylase
water
Figure
Figure 1 1
(a) At one-minute intervals the student used a pipette to remove a small volume of the water from
the beaker. test for
✓
glucose
hot water bath at .
For the first four sampl
Explain what this colour change indicates.
It indicates a positive test for the
presence of glucose meaning
has broken down starch into maltose and it has diffused
amylase
out from the risking tubing , into water
[2 marks]
(b) The student wanted to test the water in the beaker for starch.
Describe the steps that the student should follow and explain how the student will know if
starch is present or not.
Add iodine solution
drop wise onto
samples of water .
If starch solution will turn blue if not
is
present purple ,
solution will have a brown colour
[2 marks]
5
GCSE Science Required Practical Questions
(c) Buffer solution resists change to its pH when small amounts of an acid or an alkali are added to
it. Buffer solutions can be used to keep the pH of a substance constant during an experiment.
For example, if pH 5.5 buffer solution is added to a mixture of amylase and starch solution, the
pH of the mixture will remain constant at 5.5.
The student has the following buffer solutions available: 5.5, 6.0, 6.5, 7.0, 7.5 and 8.0.
Describe how the student can adapt the visking tube experiment to investigate the effect of pH
on the action of amylase.
C The charge is the pH of solutions ,
therefore different buffer solutions
needed
containg from pH 5.5 to 8.0 With 0.5 intervals are .
0 The risking tube containing the amylase and starch solution will be
added to the different solutions .
R Repeat 3 times for each buffer solution and calculate an
average
for each
Collect
M small
sample of water in a test tube and add Benedict 's
solution and
place it in a 80°C water bath for 5 minutes .
for every for A blue colour
Repeat
'
this minute solution 7 times
every ,
.
red yellow
and orange all indicate
indicates negative for starch and , green
positive result .
Draw a
graph on the pits against earliest minute
with a positive test .
S tube concentration
The concentration of starch in the risking ,
volume and
of buffer solutions should be kept same .
[6 marks]
6
GCSE Science Required Practical Questions
Q3 A group of students investigated the effect of changing light intensity on the rate of photosynthesis
in the water plant Elodea.
Figure 1 shows the equipment set up to carry out the experiment.
cut end boiling tube
containing
W lamp beaker of water
dilute hydrogen
carbonate
solution
Elodea
Figure 1
With the lamp at different distances from the Elodea the students counted the number of bubbles
leaving through the cut end in one minute.
(a) Explain why the experiment was carried out with the lamp at different distances from the
Elodea.
Lamp distances to the plant changes the
light intensity the plant recieves .
When the lamp is farther the
light intensity will be weaker
away ,
and vice versa .
[2 marks]
(b) Explain the purpose of the beaker of water.
To provide water for photosynthesis .
[2 marks]
7
GCSE Science Required Practical Questions
(c) Explain why the Elodea was placed in dilute hydrogen carbonate solution.
Because hydrogen carbonate solution can be a
pH indicator ,
which
indicate CO2 from from
can the
production of
plant photosynthesis .
as CO2 is alkaline
[2 marks]
(d) Before the sample of Elodea used in the experiment was cut from a plant, the plant was placed
in a beaker of water in front of the lamp for two hours.
Explain why this was necessary.
before experiment
To make sure the the plant can
photosynthesis e
properly and it's not
any other external factors affecting the
experiment .
[1 mark]
