Republic of the Philippines

AURORA STATE COLLEE OF TECHNOLOGY

School of Education
Brgy. Zabali, Baler Aurora

NAME: SCORE:
COURSE/YR/SEC: DATE:

MID AND FINAL TERM EXAM IN CHEM 4 – BIOCHEMISTRY

DIRECTIONS. Choose the letter of the correct answer. Write it in the space provided before each number.

___1. What discovery paved the way for the birth of biochemistry as a science?
a. fermentation of sugar into ethanol
b. synthesis of urea from ammonium cyanate
c. in vitro analysis of yeast
d. biological catalysis of urease
e. isolation of DNA from garden pea
___2. Which of the following is NOT a spontaneous process?
a. The hydrolysis of ATP to ADP and Pi.
b. The oxidation of glucose to CO2 and H2O by an organism.
c. The catalysis of 3-phosphoglyceric acid to 2-phosphoglyceric acid.
d. The reduction of NAD+ to NADH.
e. The phosphorylation of ADP to ATP.
___3. A solution that has a pH of 1
a. has twice the hydrogen ion concentration of a solution with a pH of 2.
b. has half the hydrogen ion concentration of a solution with a pH of 2.
c. has ten times the hydrogen ion concentration of a solution with a pH of 2.
d. is a basic solution.
e. is a buffer solution.
___4. Which of the following is a cyclic structure saturated hydrocarbon?
a. C2H6 b. C2H4 c. C3H6 d. C5H12 e. C6H6
___5. A reaction will occur spontaneously if
a. there is a decrease in free energy.
b. there is an increase in entropy.
c. there is an increase in free energy.
d. energy is conserved by the system.
e. heat is absorbed by the system.
___6. Which components of biological membranes are the most numerous and is amphipathic?
a. integral membrane proteins
b. glycolipids
c. membrane steroids
d. phospholipids
e. channel proteins
___7. Which of the following is the conjugate base of the species NH4+?
a. NH3 b. NH2- c. NH52+ d. NH2- e. N3-
___8. The prevailing intermolecular forces of attraction in bromine gas is`
a. hydrogen bonding
b. London dispersion
c. dipole-dipole interaction
d. ion-dipole interaction
e. dipole-induced dipole interaction
___9. Solids generally have higher density than liquids. What factor accounts for why ice floats in water?
a. surface tension
b. cohesive force
c. vapor pressure
d. freezing point
e. water structure
___10. Which of the following corresponds to the acid dissociation equation of species HX+?
a. HX+ ↔ H + X
b. HX+ ↔ H + X+
c. HX+ ↔ H+ + X
d. HX+ ↔ H+ + X-
e. HX+ ↔ H+ + X2-
___11. The nucleotide AMP contains all of the following except
a. glycosidic bond at C1
b. phosphate group of 5’
c. adenine sugar at C1
 d. hydroxyl group at C2
e. phosphodiester link at 3’
___12. Which tautomeric form of nitrogenous bases is NOT in the most stable forms?

___13. The DNA fragment 5’CATGCA3’ has which complementary fragment as transcribed by mRNA?
a. 5’GTACGT3’
b. 3’GTACGT5’
c. 5’GUACGU3’
d. 3’GUACGU5’
e. 5’CUAGCU3’
___14. In the DNA double helix
a. guanine and cytosine bond with 2 hydrogen bonds, while adenine and thymine bond with 3 hydrogen
bonds.
b. guanine and cytosine bond with 3 hydrogen bonds, while adenine and thymine bond with 2 hydrogen
bonds.
c. guanine and cytosine bond with 3 hydrogen bonds, while adenine and thymine bond with 3 hydrogen
bonds.
d. guanine and cytosine bond with 2 hydrogen bonds, while adenine and uracil bond with 3 hydrogen
bonds.
e. guanine and cytosine bond with 3 hydrogen bonds, while thymine and uracil bond with 2 hydrogen
bonds.
___15. Calculate the minimum value of ΔS in J/K.mol for the phosphorylation of ADP to ATP if the enthalpy
change for the process is 24.5 kJ/mol and that the reaction is spontaneous at 77℃.
a. 45.5 J/K.mol b. 52.8 J/K.mol c. 60.7 J/K.mol d. 70.0 J/K.mol e. 79.4 J/K.mol
___16. Calculate the temperature at which the ΔS (-105 J/K.mol) and ΔH (-11.7 J/K.mol) of the reaction involving
nucleic acid synthesis would become spontaneous.
a. 98 K b. 111 K c. 132 K d. 150 K e. 165 K
___17. How many 3’,5’ phosphodiester linkages would be present in a linear polynucleotide containing 20
nucleotide units?
a. 18 b. 19 c. 20 d. 21 e. 22
___18. Nucleases cleave 3’,5’ phosphodiester linkages. Determine the type of bond which catalyze by the
hydrolysis of the enzyme.
a. glycosidic bond b. hydrogen bond c. polar bond d. ester bond e. peptide bond
___19. Which process drives the formation of the disaccharide fructose?
a. hydrolysis
b. oxidation
c. dehydration
d. phosphorylation
e. denaturation
___20. Determine the chiral center in the compound on the right.
a. blue
b. violet
c. brown
d. green
e. red
___21. The structure of isoleucine is on the right. How many chiral centers does it have?
a. 1 b. 2 c. 3 d. 4 e. 5
___22. How many possible optical isomers does isoleucine have?
a. 1 b. 2 c. 3 d. 4 e. 5

___23. Which of the amino acids above have basic side chain?
a. A b. B c. C d. D e. A and D
___24. Which of the amino acids above have polar uncharged side chain?
a. A b. B c. C d. D e. B and C
___25. Which of the amino acids above have nonpolar hydrophobic side chain?
a. A b. B c. C d. D e. A and D
___26. Which of the amino acids above have acidic side chain?
a. A b. B c. C d. D e. B and C
___27. Denaturation of proteins affect
I. primary structure III. tertiary structure
II. secondary structure IV. quaternary structure
a. II and III b. III and IV c. I and IV d. I and II e. III only
___28. Which of these is unique to the amino acid cysteine?
a. disulfide bridge
b. thiol group
c. neutral polar side chain
d. no α carbon
e. achiral center
___29. Which of these is unique to the amino acid glycine?
a. disulfide bridge
b. thiol group
c. neutral polar side chain
d. no α carbon
e. achiral center
___30. Which of these is unique to phenylalanine?
a. aromatic hydrocarbon group
b. sulfur aliphatic hydrocarbon
c. neutral pH side chain
d. derivative of carboxyl group
e. high pKa at pH=7
___31. Which does NOT belong to the group?
a. arachidonic acid
b. strearic acid
c. oleic acid
d. linoleic acid
e. linoleic acid
___32. Which of the following has the highest melting point?
a. lauric acid, 12 C
b. myristic acid, 14 C
c. palmitic acid, 16 C
d. stearic acid, 18 C
e. arachidic acid, 20 C
___33. One molecule of phosphoric acid can form ester bonds both to glycerol and to some other alcohol,
creating
a. phospholipid serine
b. phospatidyl choline
c. phospatidyl ester
d. phospatidyl inositol
e. phospatidic acid
___34. Reaction of long chain of carboxylic acid and long chain alcohols will form
a. waxes
b. sphingolipids
c. cholesterol
d. glycolipids
e. steroids
___35. Which of the following is the precursor of testosterone, estrogen, and progesterone?
a. steroids
b. cholesterol
c. phospholipids
d. glycolipids
e. sphingolipids
___36. The structure on the right represents which group of lipids?
a. ceramides
b. cerebrosides
c. sphingomyelin
d. estradiol
e. cardiolipin
___37. Which of the following is NOT present in the lipid bilayer?
a. sphingomyelin
b. cerebroside
c. ganglioside
d. cholesterol
e. ceramide
___38. Which structure corresponds to the hydrophilic part of fatty acids?
 a. hydrocarbon
b. alcohol
c. ester
d. carboxylic acid
e. amine

___39. Determine the relationship of set A compounds above.

a. enantiomers
b. diastereomers
c. meso compounds
d. anomers
e. epimers
___40. Determine the relationship of set B compounds above.
a. enantiomers
b. diastereomers
c. meso compounds
d. anomers
e. epimers

___41. Determine the relationship of set A compounds above.

a. enantiomers
b. diastereomers
c. meso compounds
d. anomers
e. epimers
___42. Determine the relationship of set B compounds above.
a. enantiomers
b. diastereomers
c. meso compounds
d. anomers
e. epimers
___43. Determine the relationship of set C compounds above.
a. enantiomers
b. diastereomers
c. meso compounds
d. anomers
e. epimers
___44. The most common monomer of carbohydrates is
a. manose b. glucose c. sucrose d. maltose e. galactose
___45. Monossacharides are
a. aldoses b. ketoses c. both a and b d. none of these
___46. The minimum number of carbon in a monosaccharide is
a. 1 b. 2 c. 3 d. 4 e. 5
___47. All of the following are storage polysaccharide in plants except
a. starch b. cellulose c. glycogen d. dextran e. inulin
___48. Carbohydrates occur naturally in
a. D-form b. L-form c. both a and b d. none of these
___49. Sucrose is a disaccharide made up of
a. glucose and fructose
b. glucose and lactose
c. glucose and glucose
d. glucose and galactose
e. glucose and manose
___50. All of the following are reducing sugars except
a. glucose b. maltose c. sucrose d. lactose e. fructose