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Biosignalsplux User Manual

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0% found this document useful (0 votes)

432 views159 pages

Biosignalsplux User Manual

Uploaded by

sagiitariio

We take content rights seriously. If you suspect this is your content, claim it here.

Available Formats

Download as PDF, TXT or read online on Scribd

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biosignal acquisition tool-kit for advanced research applications

user manual
biosignalsplux
User Manual

ATTENTION
please read this manual before using your
biosignalsplux product

The information contained in this manual has been carefully checked and we made every
effort to ensure its quality. PLUX reserves the right to make changes and improvements
to this manual and products referenced at any time without notice.

The word Bluetooth and its logo are trademarks of Bluetooth SIG Inc. and any use of such
marks is under license. Other trademarks are the property of their respective own.

Please check your system after receiving and before using it the first time, to confirm if it
contains all the ordered sensors, accessories and other components. Contact our customer
support if there are any variations from your original order.

Last update: 01.02.2018

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PLUX Wireless Biosignals S.A.

email: plux@plux.info
web: http://www.plux.info

Headquarters
Zona Industrial das Corredouras, Lt. 14 – 1°
2630-369 Arruda dos Vinhos
Portugal
tel.: +351 263 978 572
fax: +351 263 978 902

Lisbon Office
Av. 5 de Outubro, n° 79 – 8°
1050-059 Lisboa
Portugal
tel.: +351 211 956 542
fax: +351 211 956 546

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DISCLAIMER

biosignalsplux products are intended for use in life science education and research
applications only; they are not medical devices, nor are they intended for medical
diagnosis, cure, mitigation, treatment or prevention of disease and is provided to you “as
is”.

We expressly disclaim any liability whatsoever for any direct, indirect, consequential,
incidental or special damages, including, without limitation, lost revenues, lost profits,
losses resulting from business interruption or loss of data, regardless of the form of action
or legal theory under which the liability may be asserted, even if advised of the possibility
of such damages.

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TABLE OF CONTENTS

DISCLAIMER ....................................................................................................................... 3
1 Your biosignalsplux Kit ................................................................................................ 7
1.1 biosignalsplux Explorer..................................................................................................... 8
1.2 biosignalsplux Researcher .............................................................................................. 10
1.3 biosignalsplux Professional............................................................................................. 12
1.4 biosignalsplux Force ....................................................................................................... 14
1.5 ergoplux Explorer ........................................................................................................... 16
1.6 ergoplux Explorer ........................................................................................................... 18
2 biosignalsplux HUB .................................................................................................... 20
2.1 4-Channel Hub Specifications ......................................................................................... 21
2.2 8-Channel Hub Specifications ......................................................................................... 22
2.3 Ports and Peripheries ..................................................................................................... 23
2.3.1 Button ............................................................................................................................23
2.3.2 Analog Inputs.................................................................................................................23
2.3.3 Reference ......................................................................................................................23
2.3.4 Digital Port .....................................................................................................................24
2.3.5 Charging Port .................................................................................................................24
2.3.6 Button Behavior.............................................................................................................24
2.4 General Device Functionality .......................................................................................... 25
2.4.1 Turning on the Device ...................................................................................................25
2.4.2 Connecting the Sensors .................................................................................................25
2.4.3 Disconnecting the Sensors ............................................................................................25
2.4.4 Charging.........................................................................................................................26
2.5 Communication .............................................................................................................. 27
2.5.1 Bluetooth .......................................................................................................................27
2.5.2 USB ................................................................................................................................28
3 biosignalsplux Sensors ............................................................................................... 29
3.1 Professional Sensors ....................................................................................................... 30
3.1.1 Electromyography (EMG) ..............................................................................................30
3.1.2 Electrocardiography (ECG) ............................................................................................33
3.1.3 Electrodermal Activity (EDA) .........................................................................................36
3.1.4 Electroencephalography (EEG) ......................................................................................40
3.1.5 Accelerometer (ACC) .....................................................................................................43
3.1.6 Temperature (TMP) .......................................................................................................45
3.1.7 Respiration (PZT) ...........................................................................................................47
3.1.8 Force (FSR) .....................................................................................................................50
3.1.9 Light (LUX) .....................................................................................................................52
3.2 Advanced Sensors .......................................................................................................... 55
3.2.1 Respiration (RIP) ............................................................................................................55
3.2.2 Goniometer (GON) ........................................................................................................57
3.2.3 Load Cell ........................................................................................................................59
3.2.4 Vaginal Probe (Pelvic EMG Sensor) ...............................................................................61
3.2.5 RFID Sensor....................................................................................................................63
3.2.6 Blood Volume Pulse (BVP) .............................................................................................65
3.2.7 SpO2 ..............................................................................................................................68
3.2.8 fNIRS ..............................................................................................................................71
3.2.9 Blood Pressure Reader (BPR).........................................................................................74

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3.2.10 SpO2 Reader (OSL) ........................................................................................................76

3.2.11 Glucose Meter Reader (GMR) .......................................................................................78
4 Accessories ................................................................................................................ 82
4.1 Actuators ....................................................................................................................... 83
4.1.1 Handheld Switch ............................................................................................................83
4.1.2 Foot Switch ....................................................................................................................85
4.1.3 Light-Emitting Diode (LED) ............................................................................................87
4.2 Synchronization & Additional Digital Ports ..................................................................... 90
4.2.1 Digital Synchronization Cable ........................................................................................90
4.2.2 Multi Sync Splitter .........................................................................................................92
4.3 3rd Party Compatibilities ................................................................................................ 94
4.3.1 I/O Breakout Cable ........................................................................................................94
4.3.2 Sensor Breakout PCB .....................................................................................................95
5 ergoplux .................................................................................................................... 96
5.1 Hardware ....................................................................................................................... 96
5.2 Software ........................................................................................................................ 97
5.2.1 Video Synchronization Add-On .....................................................................................97
5.2.2 Electromyography Analysis Add-On ............................................................................100
5.2.3 Muscle Load Add-On ...................................................................................................104
5.3 Scientific Background – Muscle Load ............................................................................ 107
5.3.1 Amplitude Probability Distribution ..............................................................................107
5.3.2 Computation................................................................................................................107
6 Data Logging............................................................................................................ 110
6.1 Scheduling Offline Acquisitions .................................................................................... 111
6.1.1 Control by Trigger ........................................................................................................112
6.1.2 Stop When Full ............................................................................................................112
6.1.3 Scheduling Start & End Times ......................................................................................113
6.1.4 Setting & Clearing Acquisition Schedules ....................................................................114
6.2 Downloading Offline Acquired Data ............................................................................. 115
6.2.1 Download via Bluetooth ..............................................................................................115
6.2.2 Download via Fast USB Data Transfer Cable ...............................................................116
6.2.3 Deleting Offline Acquired Data ....................................................................................118
7 Bluetooth Setup ...................................................................................................... 119
7.1 Windows 7 ................................................................................................................... 119
7.2 Windows 8 ................................................................................................................... 122
7.3 Windows 10 ................................................................................................................. 124
7.4 Linux ............................................................................................................................ 126
7.4.1 Connecting via System Settings ...................................................................................126
7.4.2 Connecting via Terminal ..............................................................................................130
7.5 Mac OS X ...................................................................................................................... 133
8 Firmware Updates & Upgrades ................................................................................ 136
8.1 Checking Firmware Version .......................................................................................... 136
8.2 Updating or Upgrading Firmware ................................................................................. 137
9 Software & APIs ...................................................................................................... 139
9.1 OpenSignals (r)evolution .............................................................................................. 139
9.2 OpenSignals Mobile ..................................................................................................... 139
9.3 APIs .............................................................................................................................. 139
10 Troubleshooting ................................................................................................... 140

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10.1 Bluetooth ..................................................................................................................... 140

10.1.1 Windows 7 & Windows 8 ............................................................................................141
10.1.2 Windows 10 .................................................................................................................145
11 Safety & Maintenance.......................................................................................... 151
11.1 Maintenance Recommendations .................................................................................. 152
11.1.1 Transportation and Storage.........................................................................................152
11.1.2 Cleaning .......................................................................................................................153
12 Regulatory & Legal Information ........................................................................... 154
12.1 Disclaimer .................................................................................................................... 154
12.2 Guarantee of Quality & Warranty ................................................................................. 154
12.2.1 Warranty Voidance ......................................................................................................154
12.3 Contact & Support ........................................................................................................ 156
13 Datasheets ........................................................................................................... 157

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1 Your biosignalsplux Kit

Figure 1: biosignalsplux kit.

biosignalsplux systems are available in 4 different kit versions. These versions are listed
on the following pages. We recommend you to check and confirm the content of your kit
after receiving it and before using it the first time, to identify missing or wrongly shipped
components or sensors that vary from your original order. Contact our support if there are
any variations from your original order.

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1.1 biosignalsplux Explorer

Figure 2: biosignalsplux Explorer Kit

This kit contains:

• 1 wireless 4-channel biosignalsplux HUB

• 4 professional sensors of your choice
• 1 PLUX proven Bluetooth dongle
• 24 electrodes
• 1 medical grade charger
• 1 portable and rugged storage case with foam cushioning to house all the parts
• OpenSignals (r)evolution signal acquisition and visualization software (download
here)
• OpenSignals (r)evolution Real-Time Event Emulation Add-On

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Optional components, features, and add-ons1:

• 1 digital I/O port

• 8GB internal memory
• Advanced sensors (see 3.2 Advanced Sensors)
• Accessories (see 4 Accessories)
• OpenSignals (r)evolution Heart Rate Variability (HRV) Add-On
• OpenSignals (r)evolution Electromyography (EMG) Analysis Add-On
• OpenSignals (r)evolution Electrodermal (EDA) Events Add-On
• OpenSignals (r)evolution Respiration (PZT & RIP) Add-On
• OpenSignals (r)evolution Video Synchronization Add-On

1
OpenSignals (r)evolution add-ons may require specific sensors. Please check the indications given
in chapter 9.1 OpenSignals (r)evolution to confirm that you have the required sensor(s).

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1.2 biosignalsplux Researcher

Figure 3: biosignalsplux Researcher Kit

This kit contains:

• 1 wireless 8-channel biosignalsplux HUB

• 8 professional sensors of your choice
• 1 PLUX proven Bluetooth dongle
• 24 electrodes
• 1 medical grade charger
• 1 portable and rugged storage case with foam cushioning to house all the parts
• 8h personalized technical support
• OpenSignals (r)evolution signal acquisition and visualization software (download
here)

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• OpenSignals (r)evolution Real-Time Event Emulation Add-On

• OpenSignals (r)evolution Video Synchronization plug-in (pre-activated in
OpenSignals)

Optional components, features, and add-ons2:

• 8GB internal memory

• Advanced sensors (see 3.2 Advanced Sensors)
• Accessories (see 4 Accessories)
• OpenSignals (r)evolution Heart Rate Variability (HRV) Add-On
• OpenSignals (r)evolution Electromyography (EMG) Analysis Add-On
• OpenSignals (r)evolution Electrodermal (EDA) Events Add-On
• OpenSignals (r)evolution Respiration (PZT & RIP) Add-On

2
OpenSignals (r)evolution add-ons may require specific sensors. Please check the indications given in
chapter 9.1 OpenSignals (r)evolution to confirm that you have the required sensor(s).

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1.3 biosignalsplux Professional

Figure 4: biosignalsplux Professional Kit.

This kit contains:

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• OpenSignals (r)evolution signal acquisition and visualization software (download

here)
• OpenSignals (r)evolution Real-Time Event Emulation Add-On
• OpenSignals (r)evolution Heart Rate Variability (HRV) Add-On
• OpenSignals (r)evolution Electromyography (EMG) Analysis Add-On
• OpenSignals (r)evolution Electrodermal (EDA) Events Add-On
• OpenSignals (r)evolution Respiration (PZT & RIP) Add-On
• OpenSignals (r)evolution Video Synchronization Add-On

Optional components and features3:

• Advanced sensors (see 3.2 Advanced Sensors)

• Accessories (see 4 Accessories)

3
OpenSignals (r)evolution add-ons may require specific sensors. Please check the indications given in
chapter 9.1 OpenSignals (r)evolution to confirm that you have the required sensor(s).

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1.4 biosignalsplux Force

Figure 5: biosignals Force

This kit contains:

• 1 wireless 4-channel biosignalsplux HUB with 8GB internal memory

• 4 load cell sensors (200Kg per sensor)
• 1 PLUX Bluetooth dongle
• 8GB internal memory
• 1 medical grade charger
• 1 sturdy aluminium plate (45x45cm)
• OpenSignals (r)evolution signal acquisition and visualization software (download
here)
• OpenSignals (r)evolution Real-Time Event Emulation Add-On

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• OpenSignals (r)evolution Center of Mass Analysis Add-on

Optional components, features, and add-ons:

• 1 digital I/O port

• Advanced sensors (see 3.2 Advanced Sensors)
• Accessories (see 4 Accessories)
• OpenSignals (r)evolution Video Synchronization Add-On

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1.5 ergoplux Explorer

ergoplux Explorer was designed to objectively track and measure work-related stress on
workers conducting physical work processes by providing reliable data for ergonomists
to detect, prevent, and/or improve high-risk work activates. ergoplux explorer aims to
help decrease and prevent the amount of work-related musculoskeletal disorders.

This kit objectively measures muscular load according to released standards (ISO 5349-
1:2001). It uses the OpenSignals (r)evolution software to acquire and visualize data from
up to 8 electromyography sensors simultaneously and the Electromyography Analysis add-
on to extract statistical information from the acquired signals to evaluate the stress
workers are exposed to in further detail.

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This kit contains:

• 1x Wireless 4-channel hub (see specs below)

• 4x Professional sensors of your choice
• 24x Pre-gelled electrodes
• 1x Bluetooth dongle
• 1x Medical-grade charger
• 1x Portable and rugged storage case with foam cushioning to house all the parts
• OpenSignals (r)evolution signal acquisition and visualization software (download
here)
• OpenSignals (r)evolution Real-Time Event Emulation Add-On
• Muscle Load add-on for OpenSignals
• VideoSync add-on for OpenSignals
• Cross-platform APIs

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1.6 ergoplux Explorer

ergoplux Researcher was designed to objectively track and measure work-related stress
on workers conducting physical work processes by providing reliable data for ergonomists
to detect, prevent, and/or improve high-risk work activates. ergoplux explorer aims to
help decrease and prevent the amount of work-related musculoskeletal disorders.

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This kit contains:

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2 biosignalsplux HUB

Figure 6: biosignalsplux hub

The biosignalsplux hub is the 4 or 8-channel device that collects and digitizes all signals
from the sensors and accessories and transmits them via Bluetooth to the computer,
where they are recorded and visualized in real-time. The channels support up to 16-bit
resolution and 3000Hz sampling frequency per channel, i.e. up to 8 channels with 3000
16-bit samples per channel per second, or 4000Hz sampling frequency per channel when
using only up to 3 channels simultaneously.

biosignalsplux is compatible with all available biosignalsplux professional sensors (3.1

Professional Sensors), advanced sensors (3.2 Advanced Sensors), and accessories. Devices
with internal memory do also allow options for onboard data logging features, without
the of a permanent Bluetooth connection.

Features
> Designed for advanced biosignal research
> Medical-grade raw data acquisition
> Ready to use in- & out-of-the-lab
> Sensor auto-detection
> Bluetooth streaming capability for online acquisitions
> Internal memory for offline acquisitions

Intended Use for Research Applications

> Life sciences studies
> Biomedical research
> Human-Computer-Interaction
> Sports sciences & biomechanics
> Robotics & Cybernetics
> biomechanics
> Ergonomics

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2.1 4-Channel Hub Specifications

Figure 7: 4-channel biosignalsplux hub.

The 4-channel hub is available in the biosignalsplux Explorer and Force Platform kits and
enables the usage of up to 4 sensors simultaneously. The standard version of this hub
does not have an internal memory and a digital port which allows the usage of
biosignalsplux accessories (see chapter 4 Accessories for more information). However,
these features can be added to a 4-channel device. Contact our support for more
information if you need to add these features (see 12.3 Contact & Support).

For detailed specifications check the table below and the biosignalsplux datasheet at the
end of this manual (see annexes).

Specifications
Analog Inputs: 4 (16-bit per channel)
Digital Ports: none (optionally available)
Common Ground: 1
Sampling Rate: up to 3000Hz (per channel); 4000Hz for up to 3 channels
Internal Memory: none; 8GB optionally available (stores up to ~111h)
Communication: Bluetooth 2.0+EDR (Class II); USB cable to download stored data
from memory (optional extra)
Bluetooth Range: up to ~10m (in line of sight)
Battery: 700mA 3.7V LiPo rechargeable (up to 12h in continuous streaming)
Hub Size: 54x85x10mm
Weight: 45g

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2.2 8-Channel Hub Specifications

Figure 8: 8-channel biosignalsplux hub.

The 8-channel hub is available in the biosignalsplux Researcher (see 1.2 biosignalsplux
Researcher) and biosignalsplux Professional (see 1.3 biosignalsplux Professional) kits and
enables the usage of up to 8 sensors and up to 3 accessories (using the multi sync cable;
see 4.2 Synchronization & Additional Digital Ports for more information) simultaneously.

The hub included in the biosignalsplux Professional kit comes with 8GB of internal
memory. Internal memory is optionally available for the hub of the biosignalsplux
Researcher kit. Contact our support for more information if you need to add internal
memory to your device (see 12.3 Contact & Support)

For detailed specifications check the table below and the biosignalsplux datasheet at the
end of this manual (see annexes).

Specifications
Analog Inputs: 8 (16-bit per channel)
Digital Ports: 1
Common Ground: 1
Sampling Rate: up to 3000Hz (per channel); 4000Hz for up to 3 channels
Internal Memory: 8GB optionally available (stores up to ~111h) (optional for the
biosignalsplux Researcher Kit)
Communication: Bluetooth 2.0+EDR (Class II); USB cable to download stored data
from memory (optional extra)
Bluetooth Range: up to ~10m (in line of sight)
Battery: 700mA 3.7V LiPo rechargeable (up to 12h in continuous streaming)
Hub Size: 54x85x10mm
Weight: 45g

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2.3 Ports and Peripheries

Figure 9: biosignalsplux ports and the button.

2.3.1 Button
The button is used to turn the device on and off and to display device status’ using the
built-in LED (see 2.3.6 Button Behavior).

2.3.2 Analog Inputs

The biosignalsplux hub has 4 or 8 analog ports to connect up to 4 or 8 biosignalsplux or
3rd party sensors to the hub (see 3 biosignalsplux Sensors for more information about the
sensors). All channels are synchronously and simultaneously acquired.

2.3.3 Reference
The reference port allows you to connect one single lead ground cable to share a common
reference point for all sensors. However, some sensors (e.g., ECG and EEG) have their own
ground/reference electrodes, which can be used instead. A common ground connection
is, for example, useful in applications that require multiple sensors that share the same
reference point (e.g. multiple EMG sensors).

Additionally, this port is used to connect the USB adapter for the fast USB data transfer
cable to download offline acquired signal from the internal memory of biosignalsplux
devices (see 6 Data Logging and 6.2.2 Download via Fast USB Data Transfer Cable).

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2.3.4 Digital Port

The digital port enables the usage of biosignalsplux accessories listed in chapter 4
Accessories (e.g., trigger buttons to manually mark events in your acquisitions or a
synchronization cables to connect several biosignalsplux for synchronized multi-device
acquisitions).

2.3.5 Charging Port

The charging port is designed to charge your device using the medical-grade
biosignalsplux charger which comes with your kit. Please read the information displayed
in the 2.4.4 Charging section before using the biosignalsplux charger and follow the
indicated instructions when charging your biosignalsplux device(s) to prevent any damage
to the user or the system due to problems occurring during the charging process.

WARNING
Do not use the biosignalsplux device during the charging process.

2.3.6 Button Behavior

The button at the center of the biosignalsplux hub enables basic control of the device.
Please read the biosignalsplux hub datasheet to get information about the button
behavior (see 13 Datasheets).

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2.4 General Device Functionality

2.4.1 Turning on the Device

In order for the device to work, it must be turned on. Turning on the device can be done
by pressing the button in the center of the biosignalsplux hub. After being turned on, the
LED status light will blink once per second (green).

Figure 10: biosignalsplux center button.

2.4.2 Connecting the Sensors

Sensors have to be connected to channel 1 to 4 or 1 to 8 (number of channels depends
on each biosignalsplux hub) in order to work properly. Do not connect the sensors to the
digital or common ground port, unless it is stated otherwise in the sensor datasheet or in
the 3 biosignalsplux Sensors chapter. The reference electrode/ground cable should be
connected to the common ground port (see Figure 6 or Figure 11).

When connecting the sensors, pay attention to where the cables go to avoid device
damages or disrupting the user’s movements.

Channel 1 to 4 Reference

Figure 11: biosignalsplux side view.

2.4.3 Disconnecting the Sensors

Disconnect the sensors according to the recommendations displayed below, to avoid
damaging the biosignalsplux device or the sensors:

• don’t twist the sensor while disconnecting

• don’t apply upward force or pull the cable
• use minimal force to disconnect
• hold the plug firmly and pull it out
• don’t pull the cable

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2.4.4 Charging

WARNING
Do not use the biosignalsplux device during the charging process.

biosignalsplux has a battery lifetime of up to 12 hours in continuous usage. Note, that the
lifetime might vary depending on the number of active sensors and/or accessories. The
built-in LED will light up red if the battery is running low until the device stops working.

To recharge, connect the biosignalsplux charger to the device’s charging port and to a
power outlet (see Figure 12). The charging process will take approximately 2 ½ hours to
fully charge the battery. The button will light red while charging (steady light when
turned off, and flashing red when acquiring or in stand by state), and the red color will
turn off once the battery is fully charged.

Charging Port
Figure 12: Charging port.

The suitable room temperature when charging the device must be between 10°C and
35°C to prevent device or user related damages during the charging process.

WARNING
Unplug the charger from the wall outlet if the battery/device overheats (reaching 60°C)
and immediately get in contact with PLUX’s Technical Assistance.

NOTE
The red LED will be switched on if an error occurs during the charging process.

In this case, unplug the charger from the wall outlet and plug it in again to complete
the charging process correctly. Please get in contact with our support if the error
remains permanently.

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2.5 Communication

The communication with biosignalsplux devices is done via Bluetooth or USB. However,
the USB communication is used to download offline acquired data only, while Bluetooth
is used to configure the device, to acquire and transmit sensor signals in real-time to the
computer, and to download offline acquired data which is stored on the internal memory
of the biosignalsplux device.

2.5.1 Bluetooth
biosignalsplux uses Bluetooth Class II to communicate with the computer and to transmit
the acquired sensor data. Since most internal Bluetooth modules are not designed to
support high transfer rates as those are needed when acquiring and streaming signals
using biosignalsplux, we strongly recommend using the PLUX Bluetooth dongle (which
comes with your biosignalsplux kit) to prevent any communication issues or connection
losses. These dongles have been tested and verified by PLUX to work properly with any
biosignalsplux device.

The Bluetooth dongle can be found in our store if several dongles are needed (e.g. to use
biosignalsplux on several computers without having to change the Bluetooth dongle every
time another computer’s being used4).

https://store.plux.info/biosignalsplux-accessories/368-bitalino-proven-bluetooth-
dongle-820201511.html

Instructions on how to connect biosignalsplux via Bluetooth to the computer can be found
in the 7 Bluetooth Setup section.

If you’re experiencing a great number of connection loss events, reconnection problems,

missing samples or similar problems, your internal Bluetooth module might be being used
for data transmission or your Bluetooth dongle might be configured to use the wrong
Bluetooth stack. Instructions to set up the correct Bluetooth stack and to solve such
problems can be found in the 10.1 Bluetooth section of this manual

See the table on the following page for detailed Bluetooth specifications of the internal
Bluetooth module of biosignalsplux devices.

4
biosignalsplux can only be connected to one computer at a time

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Operating frequency range 2400 – 2483.5 MHz ISM Band

Modulation method GFSK (1 Mbps) P/4 DQPSK (2Mbps)
Hopping 1600 hops/s, 1 MHz channel space
Transmission power Min: -11 dBm Max: +3 dBm
Antenna peak gain (XZ-V) 0.5dBi typical
Average antenna gain (XZ-V) -0.5 dBi typical
Antenna VSWR 2 max
Bluetooth, CE, FCC, IC, Japan and South
Certifications
Korea
Table 1: Specifications of biosignalsplux devices' internal Bluetooth modules.

2.5.2 USB
The biosignalsplux USB adapter and the fast USB data transfer cable biosignalsplux
accessories allow downloading offline acquired data stored in the internal memory of
biosignalsplux devices. For this purpose, the USB adapter must be connected to the
reference port of your biosignalsplux device (see 2.3.3 Reference).

Full instructions on how to access and download data from the internal memory of your
device can be found in the chapters 6.2 Downloading Offline Acquired Data and 6.2.2
Download via Fast USB Data Transfer Cable.

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3 biosignalsplux Sensors

biosignalsplux has a large variety of compatible professional and advanced sensors. While
4 or 8 professional sensors are already included in the biosignalsplux kits, advanced
sensors can be bought additionally to extend the number of acquirable biosignals.

The entire updated list of biosignalsplux sensors can be accessed via the following link:

http://biosignalsplux.com/index.php/en/products/sensors

WARNING
Do not use damaged sensors, devices or components, as this can cause serious injuries
and device damages. Contact PLUX’s Technical Assistance to report such issues and
report malfunctioning devices or sensors.

NOTE
Please review the sensor datasheets (see annexes) for more detailed specifications.

NOTE
The sensor configuration for biosignalsplux devices are demonstrated by using PLUX’s
OpenSignals (r)evolution software. Please review the software manual for more detailed
information about how to configure your device when using OpenSignals.

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3.1 Professional Sensors

3.1.1 Electromyography (EMG)

Figure 13: EMG sensor.

Our low-noise ECG local differential triode configuration enables fast application and
unobtrusive data acquisition (although custom electrode cable configurations are
available). The state-of-the-art design of the analog frontend on this sensor is specifically
targeted at analyzing minutiae in the data. Together with the Heart Rate Variability (HRV)
plugin on our OpenSignals software, one can easily record and extract meaningful
information.

Electrode cables & sleeves

The electrode cables of this sensor can be connected to electrodes with integrated stud
connectors. The colored plastic sleeves on each electrode cable indicate which electrode
must be connected to the positive or negative electrode cable (see Table 2).

Electrode Cable + -
Sleeve Color Red Black
Table 2: Color coding of the electrode cable sleeves.

How to connect your sensor to your biosignalsplux

EMG sensors can be connected to any of the available analog inputs of your biosignalsplux
device (see 2.3.2 Analog Inputs).

This EMG sensor has no built-in reference electrode. We recommend connecting a

reference electrode the reference input of your device (see 2.3.3 Reference) which can

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then be used as reference signal for your EMG signal(s). For optimal signal acquisition,
place your ground electrode on a region of the body with low level of muscle activity.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
EMG sensor connected to. Select the channel of your EMG sensor and select EMG out of
the dropdown menu (click on the arrow in the channel’s field that appears when you
hover over the channel’s field). No additional set up is needed for the reference electrode
inside OpenSignals (r)evolution.

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 14 (here: EMG connected to channel 1).

Figure 14: EMG configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 15.

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Figure 15: biosignalsplux EMG sensor sample.

OpenSignals (r)evolution Add-On: Electromyography Analysis

The Electromyography Analysis add-on is able to extract useful statistical information from
EMG data acquired with this EMG sensor. This add-ons’ automatic onset detection
algorithm enables the analysis of each individual muscle action even, in addition to the
overall analysis of the recording session. Timings analysis is also done for each activation
to a reference muscle.

This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and can be bought additionally for the use with biosignalsplux hubs and
wearables which can be used with this EMG sensor.

Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the EMG sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/EMG_Sensor_Datasheet.pdf

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3.1.2 Electrocardiography (ECG)

Figure 16: ECG sensor.

Electrode cables & sleeves

Electrode Cable + - Reference

Sleeve Color Red Black White
Table 3: Color coding of the electrode cable sleeves.

How to connect your sensor to your biosignalsplux

ECG sensors can be connected to any of the available analog inputs of your biosignalsplux
device (see 2.3.2 Analog Inputs).

This ECG sensor has a built-in reference electrode. An additional reference electrode
which can be connected to the reference input of your device is not needed but can still
be used to work with this sensor (see 2.3.3 Reference).

How to configure your sensor in OpenSignals (r)evolution

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Open the OpenSignals (r)evolution device manager and select the device you have your
ECG sensor connected to. Select the channel of your ECG sensor and select ECG out of the
dropdown menu (click on the arrow in the channel’s field that appears when you hover
over the channel’s field). No additional set up is needed for the reference electrode inside
OpenSignals (r)evolution.

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 17 (here: ECG connected to channel 1).

Figure 17: ECG configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 18.

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Figure 18: biosignalsplux ECG sensor sample.

OpenSignals (r)evolution Add-On: Heart Rate Variability

The Heart Rate Variability add-on provides important quantitative markers related with
the sympathetic or vagal activity. This plugin enables the seamless extraction and
analysis of temporal, spectral, and non-linear parameters from sensor data acquired with
this ECG sensor (or the blood volume pulse sensor; see 3.2.6 Blood Volume Pulse (BVP)).

This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and in the cardioBAN wearable but it can also be bought additionally for the
use with biosignalsplux hubs and wearables which can be used with this ECG sensor.

Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the ECG sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/ECG_Sensor_Datasheet.pdf

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3.1.3 Electrodermal Activity (EDA)

Figure 19: EDA sensor.

Our EDA sensor is capable of accurately measuring the skin activity with high sensitivity
in a miniaturized form factor. The low-noise signal conditioning and amplification circuit
design provides optimal performance in the detection of even the most feeble
electrodermal skin response

How to connect your sensor to your biosignalsplux

EDA sensors can be connected to any of the available analog inputs of your biosignalsplux
device (see 2.3.2 Analog Inputs).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
EDA sensor connected to. Select the channel of your EDA sensor and activate this channel
for signal acquisition by clicking on the circle in the channel field (must be blue).

OpenSignals (r)evolution offers two modes that are designed for different applications for
EDA sensors that can be selected in the dropdown menu which can be accessed via the
channel field (click on the arrow in the channel’s field that appears when you hover over
the channel’s field).

The EDA option is designed to work with applications where the EDA sensor is placed, for
example, on the hand of the subject. If you plan to use the EDA sensor on the arm of a
subject, we recommend selecting the EDA.ARM configuration to ensure high quality data
acquisition.

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Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 20 (here: EDA connected to channel 1

and EDA on arm configuration connected to channel 2).

Figure 20: EDA configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 21.

Figure 21: biosignalsplux EDA sensor sample.

OpenSignals (r)evolution Add-On: Electrodermal Activity Events

The Electrodermal Activity Events add-on has been designed to compute overall statistics,
basic spectral analysis, and extract typical event-related phasic features from sensor data
acquired with this EDA sensor.

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This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and can also be bought additionally for the use with biosignalsplux hubs and
wearables which can be used with this EDA sensor.

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Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the EDA sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/EDA_Sensor_Datasheet.pdf

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3.1.4 Electroencephalography (EEG)

Figure 22: EEG sensor.

Electrode cables & sleeves

Electrode Cable + - Reference

Sleeve Color Red Black White
Table 4: Color coding of the electrode cable sleeves.

How to connect your sensor to your biosignalsplux

The EEG sensors can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs).

This EEG sensor has a built-in reference electrode cable. An additional reference
electrode which can be connected to the reference input of your device is not needed but
can still be used to work with this sensor (see 2.3.3 Reference) and useful when using
multiple EEG sensors during an acquisition. For optimal signal acquisition, place your
reference electrode on a region of the body with low level of muscle activity.

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How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
EEG sensor connected to. Select the channel of your EEG sensor and select EEG out of the
dropdown menu (click on the arrow in the channel’s field that appears when you hover
over the channel’s field). No additional set up is needed for the reference electrode inside
OpenSignals (r)evolution.

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 23 (here: EEG connected to channel 1).

Figure 23: EEG configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 24.

Figure 24: biosignalsplux EEG sensor sample.

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Sensor specifications
Please read the datasheet of the EEG sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/EEG_Sensor_Datasheet.pdf

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3.1.5 Accelerometer (ACC)

Figure 25: Accelerometer.

Our 3D accelerometer uses Micro Electro-Mechanical Systems (MEMS®) technology, and
has been especially designed taking into account the requirements of applications where
kinematics and motion measurements are required. Together with our biosignal
acquisition hardware, this sensor can measure sub-milliG accelerations and provides the
raw data of each axis as an independent quantity giving you full control.

How to connect your sensor to your biosignalsplux

The accelerometer can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs). This sensor requires up to 3 analog inputs
of your biosignalsplux (1 for each axis).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
accelerometer connected to. Select the channel(s) of your ACC sensor and select XYZ from
the dropdown menu (click on the arrow in the channel’s field that appears when you
hover over the channel field). We recommend labelling your channels accordingly to your
accelerometer configuration for better visualization purposes.

Activate each channel for signal acquisition by clicking on the circle in the channel field(s)
(circle must be blue).

An example configuration is displayed in Figure 26 (here: ACC connected to channel 1 to

3; channel 1 = x-axis; channel 2 = y-axis; channel 3 = z-axis).

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Figure 26: ACC configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 27.

Figure 27: biosignalsplux triaxial ACC sensor sample.

Sensor specifications
Please read the datasheet of the accelerometer carefully before using it the first time.
The datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/ACC_Sensor_Datasheet.pdf

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3.1.6 Temperature (TMP)

Figure 28: Temperature sensor.

Our high performance NTC sensors have been specifically developed for biomedical
applications and are meant to be used on a range of temperatures suitable for body
sensing. These sensors produce a robust, stable, and accurate output with low tolerance
values. The geometry and rapid response are also of added value for even the most
demanding applications.

How to connect your sensor to your biosignalsplux

The temperature sensor can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs)

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
temperature sensor connected to. Select the channel of your temperature sensor and
select TEMP out of the dropdown menu (click on the arrow in the channel’s field that
appears when you hover over the channel’s field).

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 29 (here: temperature sensor connected

to channel 1).

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Figure 29: TMP configuration in OpenSignals.

If configured correctly, you should be able to acquire signals similar to the signal
displayed in Figure 30.

Figure 30: biosignalsplux temperature (TMP) sensor sample.

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3.1.7 Respiration (PZT)

Figure 31: PZT sensor.

Our piezoelectric respiration sensor is an entry-level affordable option for respiratory

analysis in a wide range of applications. It has a localized sensing element that measures
displacement variations induced by inhaling or exhaling. The elastic strap is provided
with the sensor to secure it in place, and can be adjusted in length, enabling the sensor
to be applied in different anatomies (e.g. male and/or female) and body locations (e.g.
thorax and/or abdomen).

How to use this sensor

This respiration sensor is a piezoelectric sensor and the membrane is centered on the
flexible strip from where the cable comes out. As it measures respiration by means of the
bending of the strip, the more the center of the strip is over the chest contour the better.
Also, make sure the sensor fastened firmly to the chest to increase the signal quality and
reliability. Signal quality will increase if the sensor it too loose.

How to connect your sensor to your biosignalsplux

The respiration sensor can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
respiration sensor connected to. Select the channel of your respiration sensor and select
RESP from the dropdown menu (click on the arrow in the channel’s field that appears
when you hover over the channel’s field).

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Activate the respiration sensor’s channel for signal acquisition by clicking on the circle in
the channel field (must be blue).

An example configuration is displayed in Figure 32 (here: respiration sensor connected to

channel 1).

Figure 32: Respiration (PZT) configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 33.

Figure 33: biosignalsplux respiration (PZT) sensor sample.

OpenSignals (r)evolution Add-On: Respiration Analysis

The Respiration Analysis add-on add-on is a convenient way to determine respiratory rate
and other useful temporal and statistical parameters associated with the respiratory

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cycles and to provide useful information about the breathing dynamics. It is designed to
work with sensor data acquired with this piezoelectric respiration sensor (PZT) (or with
the inductive respiration sensor (RIP); see 3.2.1 Respiration (RIP) ).

This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and in the raspiBAN PRO wearable but it can also be bought additionally for
the use with biosignalsplux hubs and wearables which can be used with this respiration
sensor.

Please visit the following website for more information about this add-on:
http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the respiration sensor carefully before using it the first time.
The datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/PZT_Sensor_Datasheet.pdf

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3.1.8 Force (FSR)

Figure 34: Force sensor.

From reaction time measurement to load distribution in shoe insoles, our thin film force
sensors offer uncompromised performance in the most demanding applications. The low
profile membrane and miniaturized signal conditioning circuitry are ideal for minimally
intrusive setups. Multiple sensing area dimensions and measurement ranges are available.

How to connect your sensor to your biosignalsplux

ECG sensors can be connected to any of the available analog inputs of your biosignalsplux
device (see 2.3.2 Analog Inputs)

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
force sensor connected to. Select the channel of your force sensor and select CUSTOM
from the dropdown menu (click on the arrow in the channel’s field that appears when you
hover over the channel’s field). In the custom field of your channel, adjust the GAIN and
OFFSET that fits best to your application. Testing and readjustments of this parameters
might be needed to find the configuration that fits best your needs.

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 35 (here: force sensor connected to

channel 1).

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Figure 35: Force sensor configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 36.

Figure 36: biosignalsplux force (FSR) sensor sample.

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3.1.9 Light (LUX)

Figure 37: Light sensor.

A common need when working with biosignals is the synchronization of the recorded
data with external sources (e.g. a computer screen for visual evoked potentials). If applied
to the computer screen, our LUX sensor can be used to detect chromatic changes in the
stimuli, hence providing a synchronization source. The LUX sensor can also be useful for
optical synchronization with third-party devices (provided that the third-party device can
trigger an LED), in applications where it is important to have electrical decoupling
between devices.

How to connect your sensor to your biosignalsplux

The biosignalsplux light sensor can be connected to any of the available analog inputs of
your biosignalsplux device (see 2.3.2 Analog Inputs)

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Note, that the signal you are acquiring with this light sensor is affected by the ambient
light and other light sources that are available at your experiment setup. Therefore, it is
needed to adjust the acquired signal to fit to your application and setup.

For this, open the OpenSignals (r)evolution device manager and select the device you have
your light sensor connected to. Select the channel of your light sensor and select CUSTOM
from the dropdown menu (click on the arrow in the channel’s field that appears when you
hover over the channel’s field). In the custom field of your channel, adjust the GAIN and
OFFSET that fits best to your application. Testing and readjustments of this parameters
might be needed to find the configuration that fits best your needs.

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Activate the light sensor channel for signal acquisition by clicking on the circle in the
channel field (must be blue).

An example configuration is displayed in Figure 38 (here: light sensor connected to

channel 1).

Figure 38: Light sensor configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 39.

Figure 39: biosignalsplux light (LUX) sensor sample.

OpenSignals (r)evolution Add-On: Video Synchronization

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independent recording sources, a common problem when replaying the recording session
is the synchronization of both. This plugin was created to provide an easy way to replay
biosignal data synchronously with video using this light sensor (or the biosignalsplux LED;
see 4.1.3 Light-Emitting Diode (LED) ).

This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and the biosignalsplux Researcher kit (see 1.2 biosignalsplux Researcher), and
can also be bought additionally for the use with biosignalsplux hubs and wearables which
can be used with this light sensor.

Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the EMG sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/LUX_Sensor_Datasheet.pdf

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3.2 Advanced Sensors

3.2.1 Respiration (RIP)

Figure 40: Respiration (RIP) sensor.

This high-performance inductive respiration sensor has been specifically designed having
dynamic conditions in mind (e.g. ambulatory sensing). The sensing element is embedded
in the chest strap fabric, and spans its full length. Unlike our affordable piezoelectric (PZT)
sensor (which only measures the displacement in a localized manner), the RIP option
measures the overall displacement of the thorax or abdomen, making it more immune to
motion-induced artefacts.

How to connect your sensor to your biosignalsplux

The respiration (RIP) sensor can be connected to any of the available analog inputs of
your biosignalsplux device (see 2.3.2 Analog Inputs).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Activate the respiration sensor’s channel for signal acquisition by clicking on the circle in
the channel field (must be blue).

An example configuration is displayed in Figure 41 (here: respiration sensor connected to

channel 1).

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Figure 41: Respiration (RIP) configuration in OpenSignals.

OpenSignals (r)evolution Add-On: Respiration Analysis

The Respiration Analysis add-on add-on is a convenient way to determine respiratory rate
and other useful temporal and statistical parameters associated with the respiratory
cycles and to provide useful information about the breathing dynamics. It is designed to
work with sensor data acquired with this inductive respiration sensor (RIP) (or with the
piezoelectric respiration sensor (PZT); see 3.1.7 Respiration (PZT) ).

Please visit the following website for more information about this add-on:
http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the respiration sensor carefully before using it the first time.
The datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/RIP_Sensor_Datasheet.pdf

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3.2.2 Goniometer (GON)

Figure 42: Goniometer.

This twin-axis goniometer provides accurate data related with flexion/extension and
ulnar/radial deviation. With two attachment points to the body and a flexible beam, it
enables high-performance uncompromised operation even in dynamic scenarios, without
limiting the motion of the monitored subjects. Multiple size options are available,
enabling placement in segments ranging from a finger (e.g. intermediate – proximal
phalanges) to a leg (e.g. lower part of the femur – upper part of the tibia).

How to connect your sensor to your biosignalsplux

The goniometer can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs)This sensor requires 2 analog inputs of your
biosignalsplux.

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
goniometer connected to. Select the channels of your goniometer and select GONIO for
both channels from of the dropdown menu (click on the arrow in the channel’s field that
appears when you hover over the channel’s field).

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 43 (here: goniometer connected to

channel 1).

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Figure 43: Goniometer configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 44.

Figure 44: biosignalsplux goniometer sensor sample.

Sensor specifications
Please read the datasheet of the goniometer carefully before using it the first time. The
datasheet can be found at the end of this manual or using the following link:

http://biosignalsplux.com/datasheets/GON_Sensor_Datasheet.pdf

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3.2.3 Load Cell

Figure 45: Load cell.

This single-axis load cell is particularly suitable for handgrip, traction/compression, and
similar applications. Its sturdy design and accessories enable force measurement in many
different settings. The plug & play design provides maximum flexibility for use with our
biosignal acquisition kits.

How to connect your sensor to your biosignalsplux

The load cell can be connected to any of the available analog inputs of your biosignalsplux
device (see 2.3.2 Analog Inputs).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
load cell connected to. Select the channel of your load cell and select HANDGR from the
dropdown menu (click on the arrow in the channel’s field that appears when you hover
over the channel’s field).

Activate the respiration sensor’s channel for signal acquisition by clicking on the circle in
the channel field (must be blue).

An example configuration is displayed in Figure 46 (here: load cell connected to channel

1).

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Figure 46: Load cell configuration in OpenSignals.

OpenSignals (r)evolution Add-On: Center of Mass Analysis

The Center of Mass Analysis add-on can be used for several applications using 4 of these
sensors with the biosignalsplux Force Platform (see 1.4 biosignalsplux Force). Center of
gravity distribution, jump analysis, weight assessment and force production capacity are
just some of applications. This plugin allows you to observe, in real-time, the center of
gravity and the force produced in each moment.

This add-on is included in the biosignalsplux Force kit (see 1.4 biosignalsplux Force).

Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the load cell carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/Load_Cell_Datasheet.pdf

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3.2.4 Vaginal Probe (Pelvic EMG Sensor)

Figure 47: Vaginal probe.

This sensor is especially designed for high performance pelvic floor EMG data acquisition.
The bipolar configuration is ideal for uncompromised low-noise data acquisition, and the
raw data output provides medical-grade data enabling it to be used for advanced and
highly accurate electrophysiology research.

How to connect your sensor to your biosignalsplux

The vaginal probe for applications measuring pelvic EMG can be connected to any of the
available analog inputs of your biosignalsplux device (see 2.3.2 Analog Inputs)

This EMG sensor has no built-in reference electrode. We recommend connecting a

reference electrode the reference input of your device (see 2.3.3 Reference) which can
then be used as reference signal for your EMG signal(s). For optimal signal acquisition,
place your ground electrode on a region of the body with low level of muscle activity.

How to configure your sensor in OpenSignals (r)evolution

Activate this sensor’s channel for signal acquisition by clicking on the circle in the channel
field (must be blue).

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An example configuration is displayed Figure 48 (here: vaginal probe connected to

channel 1).

Figure 48: Vaginal probe configuration in OpenSignals.

Sensor specifications
Please read the datasheet of the EMG sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/EMG_Sensor_Datasheet.pdf

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3.2.5 RFID Sensor

Figure 49: RFID sensor.

This sensor is useful for synchronous recording of biosignal data and ID-mappable events.
Usage examples include token detection and reaction time analysis in object
manipulation tasks, and also differentiated event annotation.

How to connect your sensor to your biosignalsplux

The RFID sensor can be connected to any of the available analog inputs of your
biosignalsplux device (see 2.3.2 Analog Inputs).

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
RFID sensor connected to. Select the channel of your RFID sensor and select CUSTOM
from the dropdown menu (click on the arrow in the channel’s field that appears when you
hover over the channel’s field). In the custom field of your channel, adjust the GAIN and
OFFSET that fits best to your application. Testing and readjustments of this parameters
might be needed to find the configuration that fits best your needs.

Alternatively, you can also use the RAW option, which will display the raw digital signals
that are received from the biosignalsplux.

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 50 (here: RFID sensor connected to

channel 1).

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Figure 50: Load cell configuration in OpenSignals.

Sensor specifications
Please read the datasheet of the RFID sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/RFID_Sensor_Datasheet.pdf

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3.2.6 Blood Volume Pulse (BVP)

Figure 51: BVP sensor.

This Blood Volume Pulse (BVP) sensor is an optical, non-invasive sensor that measures
cardiovascular dynamics by detecting changes in the arterial translucency. When the
heart pumps blood the arteries become more opaque, allowing less light to pass from the
emitter on the sensor through to the receiver. The BVP sensor has a plastic clip-on
housing for placement on the finger, which houses the light emitter and detector, and
also minimizes interferences from external light sources.

How to connect your sensor to your biosignalsplux

The blood volume pulse sensor can be connected to any of the available analog inputs
of your biosignalsplux device (see 2.3.2 Analog Inputs)

This sensor does not require an additional reference electrode.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
blood volume pulse sensor connected to. Select the channels of your sensor and select
BVP for the channel from of the dropdown menu (click on the arrow in the channel’s field
that appears when you hover over the channel’s field).

Activate this channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

An example configuration is displayed in Figure 52 (here: blood volume pulse sensor

connected to channel 1).

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Figure 52: Blood volume pulse sensor configuration in OpenSignals.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 53.

Figure 53: biosignalsplux blood volume pulse (BVP) sensor sample.

OpenSignals (r)evolution Add-On: Heart Rate Variability

The Heart Rate Variability add-on provides important quantitative markers related with
the sympathetic or vagal activity. This plugin enables the seamless extraction and
analysis of temporal, spectral, and non-linear parameters from sensor data acquired with
this BVP sensor (or the ECG sensor; see 3.1.2 Electrocardiography (ECG)).

This add-on is included in the biosignalsplux Professional kit (see 1.3 biosignalsplux
Professional) and in the cardioBAN wearable but it can also be bought additionally for the

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use with biosignalsplux hubs and wearables which can be used with this BVP sensor.
Please visit the following website for more information:

http://www.biosignalsplux.com/index.php/en/software/add-ons

Sensor specifications
Please read the datasheet of the blood volume pulse sensor carefully before using it the
first time. The datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/BVP_Sensor_Datasheet.pdf

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3.2.7 SpO2

Figure 54: SpO sensor (versatile).

Figure 55: SpO2 sensor with finger clip.

The SpO2 (peripheral capillary oxygen saturation) sensor uses two emitting LED’s one in
the red region and the other in the infrared region of the spectrum. The reflected light of
each one of these LED’s is absorbed by a photodiode that converts this current into a
digital value that is sent via SPI. This sensor can be used to estimate the oxygen
saturation level on the blood with +/- 2% accuracy compared to a medical sensor.

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Firmware requirements
The fNIRS sensor requires a specific biosignalsplux firmware version (v.3.4) to work. Please
follow the instructions in chapter 8 Firmware Updates & Upgrades to learn how to check
the current firmware version of your device and to upgrade or downgrade the firmware
to version 3.4. if another version is currently being used on your device.

You can download the correct firmware here (v.3.4 for SpO2 & fNIRS):

http://biosignalsplux.com/en/software/utilities

After checking and, if required, downgrading or upgrading your device’s firmware, remove
your device from the OpenSignals (r)evolution device manager and your computer’s
Bluetooth list and reconnect your device inside OpenSignals (r)evolution. If the firmware
change has been successfully, your device should now be displayed with an orange
biosignalsplux in your device manager (see Figure 56).

Figure 56: biosignalsplux device panel with firmware 3.4.

How to connect your sensor to your biosignalsplux

The SpO2 sensor(s) have to be connected to the ground port of your device. Connecting
this sensor to an analog input of your device will not work.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
SpO2 sensor connected to.

In the device’s panel, set up channel 9 for signal acquisition, set the port (P) to 9 and the
channel (C) to 1 and select one of the available SpO2 options in the channel’s dropdown
menu. The different options are designed for different placement areas of the SpO2
sensor. We recommend selecting the available configuration that fits best your needs.
The following configurations are available:

• SPO2.ARM for applications where the sensor is placed on the subject’s arm
• SPO2.FING for applications where the sensor is placed on the subject’s finger
• SPO2.HEAD for applications where the sensor is placed on the subject’s head

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Figure 57: biosignalsplux SpO2sensor sample.

Sensor specifications
Please read the datasheet of the SpO2 sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://www.biosignalsplux.com/datasheets/SpO2_Sensor_Datasheet.pdf

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3.2.8 fNIRS

Figure 58: biosignalsplux fNIRS sensor.

The fNIRS (functional near-infrared spectroscopy) sensor measures the red and infrared
light reflectance in the cortical tissue. This sensor is typically applied on the forehead
and it can be used to estimate the blood oxygen saturation level in the brain tissue.
Possible applications include brain activity monitoring, functional neuroimaging and
advanced BCI systems.

Firmware requirements
The fNIRS sensor requires a specific biosignalsplux firmware version (v.3.4) to work. Please
follow the instructions in chapter 8 Firmware Updates & Upgrades to check the current
firmware version of your device and to upgrade or downgrade the firmware to version
3.4. if another version is currently being used on your device.

You can download the correct firmware here (v.3.4 for SpO2 & fNIRS):

http://biosignalsplux.com/en/software/utilities

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Figure 59: biosignalsplux device panel with firmware 3.4.

How to connect your sensor to your biosignalsplux

The fNIRS sensor needs to be connected to the ground port of your device. Connecting
this sensor to an analog input of your device will not work.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
fNIRS sensor connected to.

In the device’s panel, set up the channels 9 and 10 for signal acquisition and set the
sensor type to fSpO2 or, if not available, to SpO2.HEAD (click on the arrow in the channel’s
field that appears when you hover over the channel’s field). For channel 9, set the port
(P) to 9 and the channel (C) to 1. For channel 10, set the port (P) to 9 and the channel to
(C) 2.

Channel 9 visualizes the reflected light of the red LED, while channel 10 visualizes the
reflected light of the IR. The intensities of the LEDs can be configured individually by
changing the sliders at the left side next to the channel configuration panels. If
configured correctly, the device panel should be similar to Figure 60.

Figure 60: Configuration of the fNIRS sensor with biosignalsplux.

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Figure 61: biosignalsplux SpO2sensor sample.

Sensor specifications
Please read the datasheet of the fNIRS sensor carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/fNIR_Sensor_Datasheet.pdf

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3.2.9 Blood Pressure Reader (BPR)

Figure 62: Blood pressure reader.

This user-friendly upper arm cuff-based blood pressure monitor measures systolic &
diastolic blood pressure levels (in mmHg) with ± 3mmHg accuracy. The built-in colour
LCD display displays all measured blood pressure values and additional heart rate
information (bpm) and allows this sensor to be used both as standalone device or as
biosignalsplux sensor within our OpenSignals (r)evolution software.

NOTE
This sensor is an adaptation of the 3rd party CK101 Wrist Blood Pressure Monitor which
provides compatibility with biosignalsplux devices. It can be used both as standalone
device or as biosignalsplux sensor.

Please do also read the datasheet of the standalone device before using this sensor:
http://biosignalsplux.com/datasheets/telemedicine/Goodwill_Studio_Ver1.pdf

How to connect your sensor to your biosignalsplux

Connect the BPR sensor to the adapter box. When done, connect the outgoing cables to
any available analog inputs of your biosignalsplux (see 2.3.2 Analog Inputs). Note that this
sensor has individual channels for the diastolic and systolic signals.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
BPR sensor connected to. Select the channel of your diastolic signal channel and set the

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sensor type to DIA (click on the arrow in the channel’s field that appears when you hover
over the channel’s field and select DIA from the dropdown menu). Select the channel of
your systolic signal channel and set the sensor type to SYS5.

Activate both channels for signal acquisition by clicking on the circle in the channel field
(must be blue).

How to use your sensor

This sensor is designed to measure blood pressure levels at the wrist. Place the sensor at
the wrist of the user (ideally the left wrist) such that both the sensor’s display and the
hand palm are facing towards the user. Please do also check the instructions provided on
the cuff to ensure optimal acquisition results.

Signal acquisitions in OpenSignals (r)evolution are done by initializing the signal

acquisition in OpenSignals and pressing the On/off button on your sensor. The cuff will
start to fill itself with air and the sensor will conduct the blood pressure measurment.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 63. Note that the sensor will achieve different states during the measurement
which can be seen in the acquires signal:

A. Sensor is not turned on but not conducting a measurement

B. Sensor cuff is being filled with air; ongoing blood pressure measurement
C. Sensor results for diastolic and systolic blood pressure values

Figure 63: Blood pressure reader sample.

5
The sensor types SYS (systolic) and DIA (diastolic) are only supported by OpenSignals (r)evolution versions
of 30.01.2018 or newer.

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3.2.10 SpO2 Reader (OSL)

Figure 64: SpO2 reader.

This comfortable and user-friendly finger clip sensor was designed to measure reliable
oxygen saturation levels (%) and heart rate (bpm) information. The built-in colour LCD
display displays all measured values and signals and allows this sensor to be used both
as standalone device or as biosignalsplux sensor within our OpenSignals (r)evolution
software.

NOTE
This sensor is an adaptation of the 3rd party CMS-50D Plus which provides compatibility
with biosignalsplux devices. It can be used both as standalone device or as
biosignalsplux sensor.

Please do also read the datasheet of the standalone device before using this sensor:
http://biosignalsplux.com/datasheets/telemedicine/CMS-50D_Plus.pdf

How to connect your sensor to your biosignalsplux

Connect the sensor to the adapter box using the micro-USB cable which comes pre-
connected to the adapter box. When done, connect the outgoing cables to any of the
available analog inputs of your biosignalsplux (see 2.3.2 Analog Inputs). Note that this
sensor has individual channels for the SpO2 and heart rate signals.

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
OSL sensor connected to. Select the channel of your SpO2 signal and set the sensor type

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to OXI (click on the arrow in the channel’s field that appears when you hover over the
channel’s field and select OXI from the dropdown menu). Select the channel of your heart
rate signal channel and set the sensor type to HR6.

Activate both channels for signal acquisition by clicking on the circle in the channel field
(must be blue).

How to use your sensor

This sensor is designed to measure oxygen saturation levels and heart rate on the index
finger.

To start acquiring a signal with this sensor when using OpenSignals (r)evolution, start the
signal acquisition in OpenSignals and hold the On/off button at the top side of your sensor
until the display is turned on. The sensor data will no be visible in OpenSignals (r)evolution.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 65.

Figure 65: SpO2 reader sample.

6
The sensor types OXI (oxygen saturation level) and HR (heart rate) are only supported by OpenSignals
(r)evolution versions of 30.01.2018 or newer.

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3.2.11 Glucose Meter Reader (GMR)

Figure 66: Glucose meter reader.

This user-friendly glucose meter measures reliable glucose level data (mg/dL) using
blood samples taken from fingertips, palms, forearms, upper arms, or calves. The built-in
colour LCD display displays the measured glucose level and allows this sensor to be used
both as standalone device or as biosignalsplux sensor within OpenSignals (r)evolution
software. This sensor comes with 10 disposable single-use lancets and test strips to take
and prepare the needed blood samples.

NOTE
This sensor is an adaptation of the 3rd party CareSensTM II Blood Glucose Monitoring
System (i-SENSO, Inc) which provides compatibility with biosignalsplux devices. It can be
used both as standalone device or as biosignalsplux sensor.

Please do also read the datasheet of the standalone device before using this sensor:
http://biosignalsplux.com/datasheets/telemedicine/caresens_ii.pdf

How to connect your sensor to your biosignalsplux

Connect the sensor to the adapter box using the 3.5mm connector which comes pre-
connected to the adapter box. When done, connect the outgoing UC-E6 cable to any of
the available analog inputs of your biosignalsplux (see 2.3.2 Analog Inputs).

How to configure your sensor in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select the device you have your
GMR sensor connected to. Select the channel of your sensor and set the sensor type to

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GLUC7 (click on the arrow in the channel’s field that appears when you hover over the
channel’s field and select GLUC from the dropdown menu).

Activate the channel for signal acquisition by clicking on the circle in the channel field
(must be blue).

How to use your sensor

This sensor is designed to measure the glucose concentration in 5µL blood samples taken
from fingertips, palms, forearms, thighs, upper arms, or calves.

WARNING
The test strips, lancets, and the disinfection cloths which come with your sensor are
disposables and are intended for single use only.

Do not reuse test strips, lancets, and the disinfection cloths and dispose them after
being used.

To start acquiring a signal with this sensor in OpenSignals (r)evolution, disconnect the
sensor from your biosignalsplux and start the signal acquisition to avoid starting with
measurement results from previous acquisitions (for more details see also the indications
on the next page).

Follow the application instructions on the flyer provided with your sensor to ensure a
correct and harmless use of this sensor during the preparation of the blood sample and
the measurement.

Prepare your blood sample and use the test strip, as indicated on the flyer to measure the
glucose level of the blood sample. The sensor requires 5 seconds to measure the glucose
level; a countdown will be shown on the device display. The test strip must be held in
the blood sample during the entire 5 seconds of the measurement process. If no error
occurred, the device will now show the measured value on its display.

After a successful measurement, plug-in the biosignalsplux connector to your glucose

meter reader. The device’s display should now be displaying “Pc” and the signal in
OpenSignals will now be at the measured value.

If configured correctly, your acquired signals should be similar to the signal displayed in
Figure 67.

7
The sensor type GLUC (glucose level) is only supported by OpenSignals (r)evolution versions of 30.01.2018
or newer.

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Figure 67: Glucose meter reader sample.

Important Notes & Troubleshooting

Initial values & effects of previous acquisitions

When using this sensor with biosignalsplux, the value of the last measurement will be
provided by the sensor and displayed in OpenSignals (r)evolution. The signal stays constant
on the last measured value and only changes when new measurements are made.

This can cause your signal to start at the value of a previous measurement even if no
actual measurement has been made during the acquisition session. To prevent initial
values of previous acquisitions, disconnect the adapter of this sensor from the
biosignalsplux and the sensor, measure the glucose level in your blood samples as
indicated on the previous page, and connect the biosignalsplux and the sensor when done.

This will cause the sensor signal to start at 0 and rise to the newly measured result.
Results of previous measurements will not be displayed in the current signal.

Signal does not change in OpenSignals (r)evolution after new measurements

After a successful measurement, the device should be displaying the measured value on
its display. Connect the sensor with biosignalsplux using the adapter for this sensor. The
display of the sensor should now be showing “Pc” and the signal in OpenSignals
(r)evolution should be adjusted to the newly measured value.

If you are experiencing no signal changes after new measurements, disconnect the sensor
from the adapter (the adapter can stay connected to the biosignalsplux). Restart your
sensor by pressing the “M” two times and wait until the last measured value is displayed
on its display. Reconnect the sensor with the biosignalsplux adapter (sensor display

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should be showing “Pc”) and the signal will now be adjusted according to the last
measured value.

Error Codes
The sensor will provide error codes if measurements could not be made correctly.
Depending on the type of error, the sensor will display one of the different error codes
below:

Error Code Issue Solution

Er1 The test strip you are trying to use
Dispose the used test strip and use a
has already been used. new test strip. Dispose the used lancet
and use a new one for the new
measurement.
Er2 The blood sample has been applied Dispose the used test strip and Use a
before the sensor has been ready new test strip and wait for the lancet
for measurements. symbol to appear at the bottom of the
display before applying your blood
sample. Dispose the used lancet and use
a new one for the new measurement.
Er3 Temperature recommended system Take the system where the temperature
range during test. is within the operating range (10-40°)
and repeat test after 30 minutes.
Er4 The blood sample has an Repeat the test with a new test strip and
abnormally high viscosity or adequate blood sample. Dispose the
insufficient volume. used lancet and use a new one for the
new measurement.
If required, increase the depth of the
lancet and massage the fingertip before
taking the blood sample, to increase the
size of the blood sample.
Table 5: Glucose meter reader error codes.

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4 Accessories

NOTE
biosignalsplux Accessories need to be connected to the digital port of biosignalsplux
devices. Please check if your device does have a digital port before considering using
one of the following accessories (4-channel biosignalsplux devices do not have a digital
port (can be added additionally); 8-channel biosignalsplux devices do have a digital
port).

NOTE
The configurations for biosignalsplux accessories are demonstrated by using PLUX’s
OpenSignals (r)evolution software. Please review the software manual for more detailed
information about how to configure your device when using OpenSignals.

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4.1 Actuators

4.1.1 Handheld Switch

Figure 68: Handheld switch.

This trigger device is useful to begin acquisition via Bluetooth to OpenSignals (or any
other custom software) or to manually start offline acquisitions stored in the hub’s
internal memory when no Bluetooth connection is available.

How to connect your accessory to your biosignalsplux

The handheld switch needs to be connected to the digital port of your device (see 2.3.4
Digital Port).

How to configure your handheld switch in OpenSignals (r)evolution to mark events

Input devices, such as the handheld switch, do not require to be configured in OpenSignals
(r)evolution if their intended use is to mark specific actions during an acquisition. In this
case, their input state can be seen in OpenSignals’ real-time acquisition mode in the
information bare located right below the acquired signals (circle to the left of INPUT
DIGITAL). The digital input circle will turn blue if a digital input has been received
(handheld switch pressed) or be kept empty if no digital input has been received
(handheld switch not pressed; see Figure 69)

Figure 69: Digital input states.

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The received inputs will be visualized in an extra channel named IO1 in the visualization
mode.

How to configure your handheld switch in OpenSignals (r)evolution to trigger acquisitions

If the intended use of the handheld switch is supposed to trigger the start of an
acquisition, it is needed to turn on this feature in the device manager. For this, open the
OpenSignals (r)evolution device manager and select the device you have your handheld
switch connected to. In the device’s panel, click on the circle next to TRIGGER to activate
the trigger feature (circle must be blue; see Figure 70).

Figure 70: Trigger configuration.

In the information bar of OpenSignals’ real-time acquisition mode(located right below the
acquired signals), the state of the trigger feature is displayed (see Figure 71).

Figure 71: Trigger state.

Accessory specifications
Please read the datasheet of handheld switch carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/BTN_Sensor_Datasheet.pdf

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4.1.2 Foot Switch

Figure 72: Foot switch.

How to connect your accessory to your biosignalsplux

The foot switch needs to be connected to the digital port of your device (see 2.3.4 Digital
Port).

How to configure your foot switch in OpenSignals (r)evolution to mark events

Input devices, such as the foot switch, do not require to be configured in OpenSignals
(r)evolution if their intended use is to mark specific actions during an acquisition. In this
case, their input state can be seen in OpenSignals’ real-time acquisition mode in the
information bare located right below the acquired signals (circle to the left of INPUT
DIGITAL). The digital input circle will turn blue if a digital input has been received (foot
switch pressed) or be kept empty if no digital input has been received (foot switch not
pressed; see Figure 73).

Figure 73: Digital input states.

The received inputs will be visualized in an extra channel named IO1 in the visualization
mode.

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How to configure your foot switch in OpenSignals (r)evolution to trigger acquisitions

If the intended use of the foot switch is supposed to trigger the start of an acquisition, it
is needed to turn on this feature in the device manager. For this, open the OpenSignals
(r)evolution device manager and select the device you have your foot switch connected
to. In the device’s panel, click on the circle next to TRIGGER to activate the trigger feature
(circle must be blue; see Figure 74).

Figure 74: Trigger configuration.

In the information bar of OpenSignals’ real-time acquisition mode (located right below
the acquired signals), the state of the trigger feature is displayed (see Figure 75).

Figure 75: Trigger state.

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4.1.3 Light-Emitting Diode (LED)

Figure 76: LED.

The LED is typically used to provide visual feedback to the user. A common need when
working with biosignals is the synchronization of the recorded data with external
recording devices (e.g. a video camera). If applied to a camera lens, the LED sensor can
be used to introduce common markers in the recording, hence providing a
synchronization source. The LED sensor can also be useful for optical synchronization
with third-party devices (provided that the third-party device has a photo detector), in
applications where it is important to have electrical decoupling between devices.

How to connect your accessory to your biosignalsplux

The LED needs to be connected to the digital port of your device (see 2.3.4 Digital Port).

How to control your LED in OpenSignals (r)evolution

Output devices, such as the LED, do not require to be configured in OpenSignals
(r)evolution. However, it is possible to select their initial state which can later be changed
in the information bar of OpenSignals’ real-time acquisition mode which is located right
below the acquired signals (circle to the right of DIGITAL OUTPUT).

To select an initial state, open the OpenSignals (r)evolution device manager and select the
device you have your LED connected to. In the device’s panel, click on the circle next to
DIGITAL to turn on (blue circle) or off (empty circle) the LED for the start of your signal
acquisition (see Figure 77).

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Figure 77: Initial state of the digital output (here: on).

To change the output state of the LED during the acquisition, click on the circle next to
DIGITAL OUTPUT in the information bar of the real-time acquisition mode. Here, again,
the LED will be turned on if the circle is blue and off is the circle is empty (see Figure 78).

Figure 78: Digital output states for LED.

OpenSignals (r)evolution Add-On: Video Synchronization

The Video Synchronization add-on has been designed for research applications which
involve recording data from sources other than the biosignal acquisition hardware
devices (e.g. video camera). Given that the biosignal hardware and the camera are
independent recording sources, a common problem when replaying the recording session
is the synchronization of both. This plugin was created to provide an easy way to replay
biosignal data synchronously with video using this LED (or the light sensor (LUX); see
3.1.9 Light (LUX) ).

Please visit the following website for more information about this add-on:

http://www.biosignalsplux.com/index.php/en/software/add-ons

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Accessory specifications
Please read the datasheet of LED carefully before using it the first time. The datasheet
can be downloaded here:

http://biosignalsplux.com/datasheets/LED_Actuator_Datasheet.pdf

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4.2 Synchronization & Additional Digital Ports

4.2.1 Digital Synchronization Cable

Figure 79: Sync cable & kit.

The multi sync splitter accessory meets your multi-device acquisition needs. By using
digital synchronization cable(s), this accessory allows you to connect biosignalsplux
devices with digital ports together to collect up to 24 channels at the same time (by
connecting up to 3 HUBs together).

NOTE
If you are using more than 2 devices, follow the instructions provided in 4.2.2 Multi Sync
Splitter).

How to connect your accessory to your biosignalsplux

The digital synchronization cable needs to be connected to the digital ports of the devices
that are being used for the multi-device acquisition (see 2.3.4 Digital Port).

How to configure synchronized acquisitions in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select one of the devices you want
to use for the multi-device acquisition. In the device’s panel, click on the circle next to
SYNC to turn on (blue circle) or off (empty circle) the synchronization function to
synchronize the acquisition of your devices (see Figure 80). This function must be active
to ensure synchronized multi-device acquisitions without signal drifts. Repeat this step
for the second device that is being used for the multi-device acquisition.

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Figure 80: Activate synchronization feature.

Accessory specifications
Please read the datasheet of SYNC kit carefully before using it the first time. The
datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/SYNC_Accessory_Datasheet.pdf

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4.2.2 Multi Sync Splitter

Figure 81: Multi sync splitter.

How to connect your accessory to your biosignalsplux

The multi sync splitter needs to be connected to the digital port of one of the devices
that are being used for the multi-device acquisition (see 2.3.4 Digital Port). When done,
link the other device(s) with the multi sync splitter by connecting digital synchronization
cable(s) (see 4.2.1 Digital Synchronization Cable) to the digital port(s) of the other
biosignalsplux devices and to the one of the three available inputs of the multi sync
splitter.

How to configure synchronized acquisitions in OpenSignals (r)evolution

Open the OpenSignals (r)evolution device manager and select one of the devices you want
to use for the multi-device acquisition. In the device’s panel, click on the circle next to
SYNC to turn on (blue circle) or off (empty circle) the synchronization function to
synchronize the acquisition of your devices (see Figure 82). This function must be active
to ensure synchronized multi-device acquisitions without signal drifts. Repeat this step
for all devices that are used for signal acquisition.

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Figure 82: Activate synchronization feature.

Accessory specifications
Please read the datasheet of Multi Sync-Splitter carefully before using it the first time.
The datasheet can be downloaded here:

http://biosignalsplux.com/datasheets/SYNC_Accessory_Datasheet.pdf

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4.3 3rd Party Compatibilities

biosignalsplux devices allow the usage and acquisition of data from 3rd party sensors,
using the available biosignalsplux breakout PCB. This accessory allows to connect analog
or digital third party sensors to the biosignalsplux hub or biosignalsplux wearables that lie
within the specified and compatible electric values of the biosignalsplux HUB. Please read
the breakout PCB section of this manual before before trying to use any 3rd party sensor
with biosignalsplux.

4.3.1 I/O Breakout Cable

WARRANTY WARNING
Usage of this breakout cable input interface VOIDS THE ELECTRICAL WARRANTY of
your device and sensors.

DISCLAIMER
BEWARE when using this accessory as to preserve electrical isolation of the user.

We expressly disclaim any liability whatsoever for any direct, indirect, consequential,
incidental or special damages, including, without limitation, lost revenues, lost profits,
losses resulting from business interruption or loss of data, regardless of the form of
action or legal theory under which the liability may be asserted, even if advised of the
possibility of such damages.

This accessory allows the connection, within the specified

electric values, of a wide range of analog or digital third party
sensors to the biosignalsplux hub. This allows, for instance, the
acquisition and visualization in real time on OpenSignals of a
third party sensor, using the biosignalsplux hub.

Specifications
Maximum Output Current: 200mA (with no other sensors
connected to the hub)
Input Voltage Range: 0 – 3V
Ouptput Voltage Range: 0 – 3V or ±1.5V (if reference is
GND)

Check the table below and the breakout cable datasheet (see annexes) for detailed
information about the pinout configuration for the analog ports (standard sensor
channels) and digital ports (8-channel biosignalsplux devices only) BEFORE CONNECTING
YOUR SENSOR TO YOUR DEVICE to avoid damaging your PLUX device.

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ANALOG PORT DIGITAL PORT

V- 0V V- 0V
GND 1.5V GND Digital output
V+ 3V V+ 3V
IN Analog input IN Digital input
Table 6: Pinout configuration for analog & digital ports.

4.3.2 Sensor Breakout PCB

WARRANTY WARNING
Usage of this breakout cable input interface VOIDS THE ELECTRICAL WARRANTY of
your device and sensors.

DISCLAIMER
BEWARE when using this accessory as to preserve electrical isolation of the user.

We expressly disclaim any liability whatsoever for any direct, indirect, consequential,
incidental or special damages, including, without limitation, lost revenues, lost profits,
losses resulting from business interruption or loss of data, regardless of the form of
action or legal theory under which the liability may be asserted, even if advised of the
possibility of such damages.

This accessory allows easy access, within the specified electric

values, to the pins on biosignalsplux sensors and actuators for
interfacing them with third-party systems.

Check the sensor datasheets before using this accessory to

avoid any damage of your system or your sensor(s).

ANALOG SENSOR DIGITAL SENSOR

V- GND V- GND
GND 1.5V (to sensor) GND Sensor output
V+ 3V (to sensor) V+ 3V (to sensor)
IN Sensor output IN 1.5V (to sensor)
Table 7: Pinout configuration for analog & digital ports.

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5 ergoplux

Work-related musculoskeletal disorders are a world problem, concerning health, social

and economics sectors. In fact, the high prevalence of such disorders in the spine and
upper limbs have been documented by several studies associated with to a higher number
of work absences and dissatisfaction, which leads to significant social and economic
costs.

With this in mind, we designed the ergoplux Explorer and the ergoplux Researcher kits
were designed to objectively track and measure work-related stress on workers
conducting physical work processes by providing reliable data for ergonomists to detect,
prevent, and/or improve high-risk work activates. This kit aims to help decrease and
prevent the amount of work-related musculoskeletal disorders.

5.1 Hardware

ergoplux Explorer uses the OpenSignals (r)evolution software to acquire and visualize data
from up to 8 electromyography sensors simultaneously and the Electromyography Analysis
add-on to extract statistical information from the acquired signals to evaluate the stress
workers are exposed to in further detail. Additionally, the Video Synchronization add-on
allows the users to record videos of the workers during the signal acquisition sessions to
visualize all signals synchronously with the recorded video for post-acquisition analysis
purposes (e.g. movement analysis).

ergoplux Researcher uses the OpenSignals (r)evolution software and the Muscle Load
Analysis software add-on to track the muscular stress of workers by acquiring
electromyography signals (up to 8 sensors simultaneously) and by analyzing the muscle
contraction intensities over time. The Electromyography (EMG) Analysis add-on allows
extracting statistical information from the acquired signals to evaluate the stress workers
are exposed to in further detail. Additionally, the Video Synchronization add-on allows the
users to record videos of the workers during the signal acquisition sessions to visualize
all signals synchronously with the recorded video for post-acquisition analysis purposes
(e.g. movement analysis).

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5.2 Software

ergoplux systems come with software add-ons for the OpenSignals (r)evolution software.

NOTE
It is required for this software to be installed on your computer. You can download
OpenSignals (r)evolution here:

http://biosignalsplux.com/en/software

We do also recommend to check the OpenSignals (r)evolution documentation to take

advantage of all features:

http://biosignalsplux.com/en/learn/documentation

5.2.1 Video Synchronization Add-On

Available in ergoplux Explorer kits & ergoplux Researcher Kits.

Figure 83: Video Synchronization Add-on.

Multimodal data acquisition in human studies usually involves recording data from sources
other than the biosignal acquisition hardware devices (e.g. video camera). Given that the
biosignal hardware and the camera are independent recording sources, a common
problem when replaying the recording session is the synchronization of both. This plugin

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was created to provide an easy way to replay biosignal data synchronously with video
using for example our LED accessory to provide a common event to both devices.

In use with ergoplux, this add-on is useful to synchronize video material recorded during
signal acquisition sessions in real-life applications for further movement and stress
analysis.

This add-on is only available in OpenSignals (r)evolution’s signal visualization mode.

ADD-ON CONFIGURATION

DEVICES & CHANNELS

The video synchronization add-on does not have to be configured to a specific channel
or device. The video mark that synchronizes the acquired all available signals with the
selected video. It affects all acquisition channels equally.

VIDEO PATH
Click on the folder next to the video field and select the acquisition video.

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SYNC TIMES
Sync times displays the information of the video mark which synchronizes the video at
the indicated time with a selected point in the acquired signals (see detailed information
below).

Video: Locked synchronization point in the video timeline

Devices: Locked synchronization point in the acquired signals

Locked synchronization point

The lock marks the synchronization point between the video and the acquired
signals. Stop the video at the time point where the synchronization input (e.g.
visual input via LED) was set in the video. Move the lock to the time point in your
acquisition where the acquired signal overlap with your recorded video and click
on the lock fix this point as the synchronization point between your video and
your acquisitions.

Current position

The aim icon uses the locked synchronization point as a reference point and
displays the current position of your acquired signals compared to the acquisition
video. It will only be displayed in the signal window if the displayed signals lie
within the synchronized video interval.

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5.2.2 Electromyography Analysis Add-On

Available in ergoplux Explorer kits.

Figure 84: OpenSignals EMG Analysis Add-On

Muscle activity is usually assessed using temporal and spectral features. With this plugin,
you will be able to extract useful statistical information from Electromyography
(EMG) sensor data. Its automatic onset detection algorithm enables the analysis of each
individual muscle activation event, in addition to the overall analysis of the recording
session. Timings analysis can also be done for each activation relative to a reference
muscle activation.

The EMG analysis add-on offers the possibility to process all available EMG channels
(hidden and visible channels), all visible channels of the acquisition, or to select a specific
device and channel to be processed by this add-on. This add-on is only available in
OpenSignals (r)evolution’s signal visualization mode.

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ADD-ON CONFIGURATION

Figure 85: EMG add-on.

DEVICES & CHANNELS

The EMG analysis add-on offers the possibility to process all available EMG channels
(hidden and visible channels), all visible channels of the acquisition, or to select a specific
device and channel to be processed by this add-on. Note that processing all visible
channels will also process non-EMG sensor data, however, this can be useful to process
RAW EMG channels.

Additionally, it can be selected weather the channels units should be set to microvolt or
millivolt in the UNIT dropdown menu.

ASSIGN DIGITAL TRIGGER

The processing interval selects the time interval of the EMG data that is to be processed.
It can be changed by manually inputting the start and end point of the acquisition session
into the time fields in the configuration window or by changing the size of the
visualization interval in the overview window.

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ASSIGN DIGITAL TRIGGER

In the ASSIGN DIGITAL TRIGGER options it is possible to select if the add-on should find
all available muscle activations or if it should find activations depending on digital input
and output signals, i.e. trigger signals.

The FIND ACTIVATIONS FROM DIGITAL ON TO DIGITAL OFF option will find all activations
within the time frames where digital inputs have been received, for example, during the
time frame a pushbutton has been being pressed.

The FIND ACTIVATIONS FROM DIGITAL ON TO … SEC will find all activations within the
specified duration after the first digital inputs received, for example, beginning at the
moment a pushbutton has been pressed.

ADVANCED OPTIONS - Filters

The advanced options allow you to change the parameters which are being used in the
activation detection algorithm to adjust the processing to your acquisition. In this option,
you can select if you want to use a lowpass, highpass or a highpass to be applied on your
signal and set the respective cutoff frequencies for the selected filter.

To use a lowpass filter, click on the LOWPASS button and enter the preferred cutoff
frequency for this filter in the CUTOFF – LOW field and enter the order of the filter in the
ORDER field.

To use a highpass filter, click on the HIGHPASS button and enter the preferred cutoff
frequency for this filter in the CUTOFF – HIGH field and enter the order of the filter in the
ORDER field.

To use a bandpass filter, click on the BANDPASS button and enter the preferred cutoff
frequency for the lowpass component of this filter in the CUTOFF – LOW field and enter
the preferred cutoff frequency for the highpass component of this filter in the CUTOFF –
HIGH. Enter the order of the filter in the ORDER field.

ADVANCED OPTIONS – Process X Channel

This option allows you to apply the statistical algorithms of this add-on to a selected
channel X within the same time intervals of another channel without any activation
dependency of channel X. The add-on will search for activations on a selected EMG
channel (click on the channel to select) and analyze the selected channel X at the same
time intervals where activations have been found in the selected EMG channel even if
there are no activations or activation triggering activities within these time intervals in
channel X (e.g. muscle is relaxed in during this interval).

With this option it is possible to compare two EMG signals within the same time intervals
without any activation dependencies.

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MCV – MAXIMUM VOLUNTARY CONTRACTION

The maximum voluntary contraction (MVC) will be calculated for each channel based on
the EMG maxima of your selected EMG signals.

The MVC acquisition can also be done in a separate signal acquisition session/file. This
can be useful to use when doing several signal acquisitions to ensure that the analysis of
all files and signals are done with the same reference values – the MVC values.

For this, start an acquisition and acquire your data which are being used as MVC values
for later analysis and save your acquisition. To use the MVC data from this acquisition in
another acquisition, click on the document icon in the CALCULATE FROM A FILE INSTEAD
field and select the OpenSignals (r)evolution file which contains the MVC signal data.
OpenSignals (r)evolution will then calculate the MVC value from the selected file.

Select OpenSignals (r)evolution file to calculate MVC values from file

Each channel’s MVC value are listed in the table below MAXIMUM VOLUNTARY
CONTRACTION.

MUSCLE ACTIVATION RESULTS

Click on the PROCESS button to process all signals according to the set configuration and
all found activations will be visualized in the processed channels (white frames).

To display all statistical information about one or two specific activations, click on the
first activation (left mouse button). The white frame turns into a blue frame and all
statistical information about this activation will be listed in the ACT X column of the
analysis results. When done, select the second activation of your choice by holding the
CTLR key of your keyboard and click on the activation (left mouse button). The white
frame turns into a green frame and all statistical information about this activation will be
listed in the ACT Y column of the analysis results.

The results table displays general information about the source of this signal (device &
channel) as well as general information about the duration and times of your acquisition.
All activation specific data is displayed below the given general information (e.g.,
activation number, duration).

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5.2.3 Muscle Load Add-On

Available in ergoplux Explorer kits & ergoplux Researcher Kits.

Figure 86: OpenSignals Muscle Load Add-On.

This add-on evaluates the muscular load that the muscles are subjected during a normal
work day in real-time and after signal acquisition sessions. It measures the static, median
and high intensity levels and displays the amplitude probability density function which
helps detecting weather the worker is working within ranges that are safe or risky.

This add-on works both offline and online. It is available in OpenSignals (r)evolution’s
signal visualization mode and in the signal acquisition mode.

NOTE
For detailed information about the scientific background of this add-on, please check
the section 5.3 Scientific Background – Muscle Load.

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ADD-ON CONFIGURATION

Figure 87: Muscle Load add-on.

DEVICES & CHANNELS

The Muscle Load add-on processes all available and active channels.

Active channels are all channels which have not been hidden and are therefore visualized
in the signal visualization mode.

MVC – MAXIMUM VOLUNTARY CONTRACTION

At the beginning of each acquisition it is needed calibrate the add-on in order for it to
provide reliable data. To do so, click on the MVC button (maximum voluntary contraction)
and let the subject contract the muscle which is being monitored to its maximum. Make
sure the muscle can be contract without any restrictions for the calibration to be done
properly. This contraction will provide the value of the maximum possible contraction of
the muscle. This value will be the reference point for the signal acquisition and
processing. Each channel’s MVC value are listed in the table below MVC – MAXIMUM
VOLUNTARY CONTRACTION.

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The maximum voluntary contraction (MVC) will be calculated for each channel based on
the EMG maxima of your selected EMG signals.

Select OpenSignals (r)evolution file to calculate MVC values from file

APDF – AMPLITUDE PROBABILITY DENSITY FUNCTION

After this acquiring the MVC, click on the APDF button to start the amplitude probability
density function. This function calculates the cumulative percentage histogram of the
enveloped data in terms (%) of the MVC and displays if the muscle load that the muscle(s)
are subjected during the current task (represented by the blue lines in the graph) are
causing muscle tiredness (see WARNING ZONE area in Figure 87) or muscle fatigue (see
DANGER ZONE area in in Figure 87). Low to no muscle load will shift the blue line to the
top left corner of the graph, will increasing muscle load and muscle load over duration
will shift the graph into the warning and danger zones.

EFFORT DENSITY (%MVC IN %TIME)

This function displays the cumulated effort in terms (%) of the MVC at 10, 50 and at 90%
of the time (blue lines on chart) and displays all values of all devices and sensors in a
table.

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5.3 Scientific Background – Muscle Load

The scientific background for the Muscle Load add-on is based on B. Jonsson’s paper from
1982 “Measurement and Evaluation of Local Muscular Strain in the Shoulder During
Constrained Work”.

Please find below detailed information about this work and the Amplitude Probability
Distribution Function for deeper understanding of how the functionality of the Muscle
Load add-on.

5.3.1 Amplitude Probability Distribution

Proper electromyography signal processing can provide a reference for the analysis of the
relationship between work and required muscular load. Jonsson (1982) described a
technique in which the frequency of occurrence of a particular level of EMG is calculated,
generating a curve of the probability distribution function of amplitude (APDF). The APDF
is the distribution of the muscle contraction levels during a certain observation period.

The APDF concept was originally developed to extract information from EMG records of
muscular activity and has been used to analyze EMG in many Ergonomics studies.

5.3.2 Computation
The determination of APDF allows to obtain the profile of muscle tension that workers
are subject during a work period. The determination process involves the following
sequence:

• Calculation of the envelope of the EMG signal obtained by rectifying and

smoothing using a low pass filter.

• Determination of the histogram of the EMG signal amplitude for each muscle
during the execution of the task to be studied relative to the maximum voluntary
contraction (MVC) of the same muscle;

• Calculation of the cumulative histogram of EMG amplitude distribution and

mapping of the probability values regarding the distribution of the muscle for
MVC. This way, the amplitude of EMG, as well as the probability distribution is
related to the MVC, which allows comparing between several muscles or between
several tasks as well as the estimation of the average APDF function groups of
workers and/or tasks.

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Figure 88: EMG signal processing - Collecting samples (A); Construct the amplitude histogram (B) and the cumulative
amplitude (C), which will be converted into an APDF curve. [Adapted from Jonsson (1982)].

The final results of the APDF processing allows to evaluate the relative intensity of
different tasks in a workstation as a whole or even the sequence realized for workers
during their turn.

The amplitude probability of a certain level of contraction is the muscle’s electrical

activity probability lower or equal than the level of contraction. The amplitude
probability of a certain level can be expressed as the time interval where the signal is
less or equal than that level. If the electromyographic activity amplitude is expressed in
percentage of the MVC’s calculated value, the amplitude probability distribution is
related to the contractions during the work day.

The contraction level of electromyographic signals, which has an amplitude distribution

probability that initiates at a probability P=0 level, indicates the lowest contractile
activity that occurred throughout the work period. This corresponds to the basal static of
contraction level which is, in most of the cases, zero or quite close to zero, especially in
long-term acquisitions. A typical situation of this type of contraction is the interruptions
between active periods of work.

The highest contraction level of EMG amplitude distribution probability curve

corresponds to a probability of P=1, indicating the highest level recorded over the work

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period. In cases where the highest value for muscle activity estimation might exceed 50%
of the MVC, the normalization of EMG signals must be performed with a direct assessment
of 100% from the MVC. Otherwise, one might establish an underestimate of APDF.

The average level of contraction associated with a probability of P=0.5 indicates the
average value of the contraction developed during the work period. Usually, this average
level tends to the average contraction value when obtained from the EMG integration or
electromyographic signal’s RMS detection.

EMG amplitude distribution probability curve will feature how muscle behaves during
study period. Each type of work presents a characteristic profile concerning the amplitude
distribution probability curve.

When electromyographic records are used to obtain muscle activity levels’ evaluation,
criteria must be taken into account in order to define which obtained values for muscle
tension levels are acceptable and non-acceptable according to the performed
occupational tasks.

According to muscle fatigue studies, when performing static and dynamic tasks, a set of
limit values were suggested for the work performed over periods longer than one hour
(Jonsson, 1982):

• Static contraction level must not exceed 2% of the MVC and cannot overcome 5%
of the MVC;

• The average level must not exceed 10% of the MVC and cannot overcome 14% of
the MVC;

• Load’s highest values must not exceed 50% of the MVC and cannot overcome
70% of the MVC.

These limit values can be used as analysis criteria of the amplitude distribution
probability curves for the relative strength in occupational tasks. One must consider the
amplitude distribution probability level for an entire work day, and the limit should not
be exceeded for over an hour in continuous work. If limit is exceeded, it must be
exceptionally and for a 10 minutes maximum period.

These criteria can only be used for electromyographic records’ analysis of a group of
workers. The individual test results within workers can exceed these limits for several
reasons, such as unfavorable operational modes selection, although workers group’s
overall workload is not excessive.

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6 Data Logging

NOTE
The following biosignalsplux features are demonstrated by using PLUX’s OpenSignals
(r)evolution software. Please review the software manual for more detailed information
for general software use.

biosignalsplux devices with internal memory allow you to schedule and manage offline
acquisitions which can be initialized and conducted without the requirement of a
Bluetooth connection with your computer and OpenSignals (r)evolution.

The following devices are compatible with data logging features (scheduling, recording,
and downloading recorded data):

• biosignalsplux Professional
• biosignalsplux Force
• respiBAN Professional

Internal memory can also be added additionally for all biosignalsplux devices. Please
contact our support via e-mail at support@plux.info if you’d like to add internal memory
to your device to use all data logging features.

Devices with internal memory are displayed with an additional SCHEDULE button at the
end of their device panels (see Figure 89). These devices are compatible with all data
logging features.

Figure 89: Data logging feature for devices with internal memory.

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6.1 Scheduling Offline Acquisitions

NOTE
Before scheduling offline acquisitions, we recommend setting up the device and
channels first. To do so, configure your device to fit your sensors and channel
configuration according to the instructions in chapter 3 biosignalsplux Sensors before
entering the schedule panel.

Click on the SCHEDULE button of your device with internal memory in the device panel
to access the scheduling panel to schedule and configure your offline acquisition (see
Figure 90).

Figure 90: Scheduling panel.

The scheduling panel allows to configure your offline acquisition by defining the type of
initiator for the start of your acquisition (timed or triggered), to stop the acquisition when
the limits of the internal memory are reached (STOP WHEN FULL), to time acquisition at a
specific time or duration (STOP and ACQUISITION), and displays information about the
available free memory and the estimated memory usage of your scheduled acquisition.

NOTE
The indications of the estimated end of the acquisitions are calculated estimations and
might vary from real-life behaviour.

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NOTE
It is recommended to fully charge your device’s battery before using offline acquisitions
as the acquisitions can be of longer period than the battery lifetime of your device, for
example when using the STOP WHEN FULL option. The estimated end will not be
reached if the battery runs out before.

NOTE
Your device does not have to be turned on before your scheduled acquisition starts, as
it will automatically be turned on at the scheduled start-point.

To check if your device is acquiring data, check the LED information of your device:
http://biosignalsplux.com/datasheets/biosignalsplux_hub_Spec_Sheet.pdf

6.1.1 Control by Trigger

This option allows you to start your acquisition manually using one of the available
trigger accessories for biosignalsplux (see 4.1.1 Handheld Switch or 4.1.2 Foot Switch). To
activate this feature, click on the circle next to CONTROL BY TRIGGER and make sure that
your trigger accessory is correctly connected to the digital port of your biosignalsplux
device.

This feature is activated if this circle is filled blue and inactive if the circle is empty.
Activating this feature will also remove every time information in the START and STOP
fields.

To set up a fix duration of your offline acquisition, set up your preferred duration in the
ACQUISITION field by using the ‘+’ and ‘-‘ buttons. The acquisition will then stop when the
duration has been passed, with the duration starting at the point where the trigger signal
has been given.

Click on the STOP WHEN FULL option to stop the acquisition only when there is no
memory left on your device. Read the following section for more information about this
option.

6.1.2 Stop When Full

The STOP WHEN FULL option will stop your offline acquisition when the internal memory
of your device has run out of available memory. This function can both used with trigger-
based or timer-based initiated offline acquisitions. If a fixed time has been selected for
the start of the acquisition, the estimated end of the acquisition will be calculated based
on the available free memory and will be displayed in the STOP field of the scheduling
panel (see Figure 91).

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Figure 91: Scheduling panel displaying the estimated end of the acquisition.

6.1.3 Scheduling Start & End Times

Figure 92: Time schedule for start and end points for offline acquisitions.

Alternatively, you can also set up a fix duration for your acquisition instead of configuring
an end time. To do so, set up your preferred duration in the ACQUISITION field by using
the ‘+’ and ‘-‘ buttons. The acquisition will then stop when the duration has been passed,
with the duration starting at the scheduled time configured in the START field.

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6.1.4 Setting & Clearing Acquisition Schedules

If you’ve finished configuring your device and your offline acquisition, click on the SET
SCHEDULE button at the bottom of the scheduling panel to transmit all the configurations
to your device. The transmission and configuration has been successful if the button at
the bottom of your device panel in OpenSignals (r)evolution has changed from SCHEDULE
to SCHEDULED followed by the day and time when the acquisition has been configured
to start (non-triggered acquisitions only) (see Figure 93; here: Tuesday 19:00h).

Figure 93: Device panel with a scheduled acquisition.

To clear a scheduled acquisition, click on the SCHEDULED button and click on the CLEAR
SCHEDULE of the scheduling panel. The scheduled offline acquisition will be removed
and your device can now be configured for a new offline acquisition.

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6.2 Downloading Offline Acquired Data

After your offline acquisition has finished, you can download the recording by clicking on
the download data button in the OpenSignals (r)evolution main screen:

Download an acquisition from the memory card

The offline can be downloaded from your device’s internal memory within OpenSignals
(r)evolution via Bluetooth or via the fast USB data transfer cable (additional accessory). The
following sections provide the instructions on how to download data using both methods.

6.2.1 Download via Bluetooth

Make sure your biosignalsplux is turned on and click on the download button in the
OpenSignals (r)evolution main screen to access the download panel. In this panel, all the
available offline acquisitions which are available for download from your device’s internal
memory are listed.

Each offline acquisition creates its own file where the acquired data is stored. In the
download panel, the files are listed with their file name (FILE) their start time (START) ant
their entire duration (DURATION). Click on the indicated arrow in a file’s row to download
the selected file (see Figure 94) or click on the arrow in the top row of the downloading
panel to download all available files. The downloaded files will be stored on your
desktop.

Figure 94: Downloading offline acquired data via Bluetooth.

NOTE
The time needed to download an acquisition file depends on the size of the file and,
therefore, on the duration of your scheduled acquisition. The download process might
need several minutes to end. Do not use nor turn off your device when OpenSignals
(r)evolution is downloading data, as doing so might make the downloaded file
unreadable.

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6.2.2 Download via Fast USB Data Transfer Cable

NOTE
This method requires accessories which have to be bought additionally (in some kits).
These accessories are available in our store.

biosignalsplux USB Adapter & fast USB data transfer cable:

https://store.plux.info/biosignalsplux-accessories/371-fast-usb-data-transfer-cable-
for-biosignalsplux-820201514.html

Make sure your biosignalsplux is turned on and connected to your computer via USB with
the biosignalsplux USB adapter. Connect the USB adapter to the reference port of your
biosignalsplux (see 2.3.3 Reference) and click on the download button in the OpenSignals
(r)evolution main screen to access the download panel. In this panel, all the available
offline acquisitions which are available for download from your device’s internal memory
are listed.

Each offline acquisition creates its own file where the acquired data is stored. In the
download panel, the files are listed with their file name (FILE) their start time (START) ant
their entire duration (DURATION). Click on the indicated USB symbol in a file’s row to
download the selected file (see Figure 95) or click on the USB symbol in the top row of
the downloading panel to download all available files. The downloaded files will be
stored on your desktop.

Figure 95: Downloading offline acquired data via USB.

Do not use nor turn off your device when OpenSignals (r)evolution is downloading data,
as doing so might make the downloaded file unreadable.

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6.2.3 Deleting Offline Acquired Data

NOTE
Deleted files cannot be restored. Make sure you have downloaded all the needed files
before deleting or erasing the internal memory of your device to prevent accidental data
loss.

To delete all files stored in the internal memory of your device, click on the ERASE CARD
button at the bottom of the download panel.

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7 Bluetooth Setup

When connecting the device, you first need to enable Bluetooth on your computer or need
to plugin the Bluetooth dongle to your USB port, if Bluetooth is not built in or reliable for
your computer. Follow the instructions for your operating system that are displayed on
the following pages to connect your biosignalsplux to your computer.

7.1 Windows 7
To connect your device to your computer via Bluetooth, click on the start button at the
right lower part of the Windows taskbar and click on Control Panel to access the control
panel of your computer.

Figure 96: Access the Windows 7 control panel.

In the control panel, select the Add a device option in the Hardware and Sound section to
add your device.

Figure 97: Select Add a device in the Hardware and Sound section.

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At this point, turn on your biosignalsplux (if not done before) and wait until it appears in
the list available devices. When your device has been found (here: biosignalsplux), click
on the device and click on Next at the lower right corner of the Add a device window.

Figure 98: Select your device to add it to your computer.

Click on Enter the device’s pairing code and click on Next at the lower right corner of the
window to proceed.

Figure 99: Select Enter the device's pairing code.

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For biosignalsplux devices enter 123 and click on Next to connect to your device. For
BITalino devices enter 1234 and click on Next to connect to your device.

Figure 100: Enter the pairing code to connect to your device.

If Windows confirms that your device has been successfully added to your computer, the
connection has been successfully established.

Figure 101: Windows confirmation if the device has been successfully added.

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7.2 Windows 8
To connect your biosignalsplux device to your computer via Bluetooth, click on the start
button at the right lower part of the Windows taskbar, click on the triangle symbol and
click on the Bluetooth icon to open the Bluetooth settings panel.

Figure 102: Access to the Bluetooth settings panel in Windows 8.

At this point, turn on your biosignalsplux (if not done before) and wait until it appears in
the list of Bluetooth devices. When your biosignalsplux device has been found, click on
the device and click on Pair to connect to your device.

Figure 103: Bluetooth settings panel.

Windows will now try to connect to your device and pop up a window requesting you to
enter the passcode (pin) of your device. Enter 123 and click on Next to connect to
biosignalsplux.

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Figure 104: Requesting biosignalsplux' passcode (123).

If Connected is displayed under the name of your biosignalsplux device in the Bluetooth
settings panel, the connection has been successfully established.

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7.3 Windows 10
To connect your biosignalsplux device to your computer via Bluetooth, click on the start
button at the left lower corner in Windows 10 to open the start menu. Click on the gear
symbol to open the settings panel of your operating system as can be seen below.

Figure 105: Access to the settings panel in Windows 10.

In the settings panel, click on the Devices Bluetooth, printers, mouse field to open the
device configurations of your system.

Figure 106: Windows 10 settings panel.

Select Bluetooth from the list which is displayed on the left side of your settings window.
Note, that in some versions the Bluetooth option might not be available and that
Bluetooth devices might be listed under Other devices as seen in Figure 107.

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Figure 107: Bluetooth settings panel.

Windows will now try to connect to your device and pop up a window requesting you to
enter the passcode (pin) of your device. Enter 123 and click on next to connect to
biosignalsplux.

Figure 108: Requesting passcode (123).

If Pair is displayed under the name of your biosignalsplux device in the Bluetooth settings
panel, the connection has been successfully established.

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7.4 Linux
Ubuntu offers two options on how to connect your devices via Bluetooth to your
computer. The first option allows you to connect the device via Ubuntu’s system settings
and the graphical interface. The second option allows you to connect the device via the
terminal. However, the second option is recommended for advanced users only.

7.4.1 Connecting via System Settings

To connect your device to your computer via Bluetooth, click on the wheel in the top right
corner of the display and click on System Settings.. to access the settings panel of your
computer.

Figure 109: Access the system settings in Ubuntu.

In the settings panel, click on the Bluetooth logo to access the Bluetooth settings panel.

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Figure 110: Click on the Bluetooth logo to access the Bluetooth settings.

At this point, turn on your biosignalsplux (if not done before) and wait until it appears in
the list available devices. When your device has been found (here: biosignalsplux), it will
be listed in the Device Search window. Click on the device and select PIN options… to enter
the device’s PIN to proceed.

Figure 111: Select your device to add it to your computer.

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In the new PIN Options window click on Custom PIN and enter the device’s PIN to connect
to your device. For biosignalsplux devices enter 123 and click on Next to connect to your
device.

NOTE
If the Custom PIN option is not available in this window, follow up with the instructions
in Connecting via Terminal to connect your device to your computer using the terminal
instead of proceeding with the configuration via the system settings.

Figure 112: Enter the device’s pin to pair.

Click on Close to close this window and click on Next on the Device Search window to
connect to your device.

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Click on your device in the Devices list. If paired is confirmed (Paired Yes) your device has
been successfully connected to your computer8.

Figure 113: Successfully paired biosignalsplux.

8
Do not worry if the connection is set off. It will automatically switch to on when connecting to your device
using OpenSignals or other custom software for biosignalsplux.

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7.4.2 Connecting via Terminal

NOTE
Using the terminal may harm your computer if it is not done correctly. Therefore, this
method is recommended for advanced users only and following this method is done at
your own risk.

To connect your device to your computer via Bluetooth, click on the Unity Dash logo in
the toolbar on the left to your desktop open the search function. In the search bar, enter
terminal (or Terminal) and click on the terminal logo to open the Terminal.

Figure 114: Access the terminal using the search function in Ubuntu.

Enter bluetoothctl into the terminal window (without the $). Terminal should answer to
this command with the a line displaying the MAC address of your internal Bluetooth
adapter. The response should be similar to this (with AA:BB:CC:DD:EE:FF being replaced
with your MAC address):

$ bluetoothhctl
[NEW] Controller AA:BB:CC:DD:EE:FF ubuntu [default]
[bluetooth]#

Next, enter turn on your internal Bluetooth module on (if not done before) by entering
power on into the Bluetooth prompt line. The response should be similar to this (again,
with AA:BB:CC:DD:EE:FF being replaced with your MAC address):

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[bluetooth]# power on
Changing power on succeeded
[CHG] Controller AA:BB:CC:DD:EE:FF Powered: yes

Next, it is needed to turn on a Bluetooth agent. The Bluetooth agents manages the
transmission of pin codes (or pairing codes) between devices and is needed here to send
the pairing code to the device you want to connect. To do this, enter agent on. The
terminal response should be similar to this:

[bluetooth]# agent on
Agent registered

Set up the default-agent to proceed with the pairing process.

[bluetooth]# default-agent
Default agent request successful

At this point, turn on your device (if not done before) and enter scan on into the terminal
to search for available Bluetooth devices and wait until your device’s MAC address
appears in the list (here: biosignalsplux, 00:07:80:B3:84:01). Your device’s MAC address
can be found at the back of the device.

[bluetooth]# scan on
Discovery started
[CHG] Controller AA:BB:CC:DD:EE:FF Powered: yes
[NEW] Device 00:07:80:B3:84:01 biosignalsplux

If your device is listed, enter pair followed by the MAC address of your device to start the
pairing process which will be respond with requesting the PIN code of your device. For
biosignalsplux devices enter 123 and click on Next to connect to your device. For BITalino
devices enter 1234 and click on Next to connect to your device. The result should be
similar to this:

[bluetooth]# pair 00:07:80:B3:84:01

Attempting to pair with 00:07:80:B3:84:01
Request PIN code
[agent] Enter PIN code: 123
[CHG] Device 00:07:80:B3:84:01 Connected: yes
[CHG] Device 00:07:80:B3:84:01 Modalias: bluetooth
[CHG] Device 00:07:80:B3:84:01 UUIDs: (device specific ID)
[CHG] Device 00:07:80:B3:84:01 UUIDs: (device specific ID)
[CHG] Device 00:07:80:B3:84:01 Paired: yes
Pairing successful
[biosignalsplux]

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If the terminal outputs Pairing successful your device has been successfully paired to your
computer.

The entire text of your terminal should now be similar to the following:

$ bluetoothhctl
[NEW] Controller AA:BB:CC:DD:EE:FF ubuntu [default]
[bluetooth]#
[bluetooth]# power on
Changing power on succeeded
[CHG] Controller AA:BB:CC:DD:EE:FF Powered: yes
[bluetooth]# agent on
Agent registered
[bluetooth]# default-agent
Default agent request successful
[bluetooth]# default-agent
Default agent request successful
Discovery started
[CHG] Controller AA:BB:CC:DD:EE:FF Powered: yes
[NEW] Device 00:07:80:B3:84:01 biosignalsplux
[bluetooth]# pair 00:07:80:B3:84:01
Attempting to pair with 00:07:80:B3:84:01
Request PIN code
[agent] Enter PIN code: 123
[CHG] Device 00:07:80:B3:84:01 Connected: yes
[CHG] Device 00:07:80:B3:84:01 Modalias: bluetooth
[CHG] Device 00:07:80:B3:84:01 UUIDs: (device specific ID)
[CHG] Device 00:07:80:B3:84:01 UUIDs: (device specific ID)
[CHG] Device 00:07:80:B3:84:01 Paired: yes
Pairing successful
[biosignalsplux]

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7.5 Mac OS X

To connect your biosignalsplux device to your computer via Bluetooth, click on the Apple
symbol at the top left corner of your display and select System Preferences…

In the system preferences window click on the Bluetooth symbol to open the Bluetooth
settings.

Figure 115: Mac OS X system preferences window.

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Figure 116: Click on Pair to connect to the biosignalsplux.

An error will occur indicating that the connection could not be established due to a
mismatching passkey. Click on Options… to enter the passkey (pairing code).

Figure 117: Click on Options… to enter the passkey.

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Mac OS X will now try to connect to your device and pop up a window requesting you to
enter the passcode (pin) of your device. Enter 123 and click on Pair to connect to
biosignalsplux.

Figure 118: Enter the pairing code

If Connected is displayed under the name of your biosignalsplux device in the Bluetooth
settings panel, the connection has been successfully established.

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8 Firmware Updates & Upgrades

biosignalsplux firmware updates are not done automatically. We recommend to frequently

check if newer firmware versions are available and to upgrade or update the firmware on
your device when newer versions are available to ensure full functionality and
compatibility with all biosignalsplux sensors and accessories.

Updating the biosignalsplux firmware lets you also take advantage of all the newest
recording resolutions and offline scheduling features.

Check the following website to see if newer firmware versions are available, and
download and extract all the required tools to update or upgrade your firmware:

http://biosignalsplux.com/index.php/en/software/utilities

NOTE
biosignalsplux devices can be used on Windows, Linux and Mac OSX operating systems.
However, at the time being, the firmware update software does only run on Windows
operating system.

For Mac OS X and Linux users, the firmware update software can be used in virtual
machines running a compatible Windows version (Windows 7 or newer).

8.1 Checking Firmware Version

Before updating the firmware of your biosignalsplux device, it is recommended to check
the current device’s firmware version to see whether a firmware update is required or not.

To do so, make sure that your device is charged and turned on (LED must blink green). If
you haven’t connected your device to your computer via Bluetooth before, connect your
device following the instructions in Bluetooth Setup before following the next steps.

Open the Updater.exe you’ve just downloaded and extracted. A new window will appear.
Click on the Find Devices button and wait for your MAC address to appear in the Device
Mac Address Field.

Figure 119: PLUX firmware update tool has found one biosignalsplux.

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Check if the MAC address that is listed in the dialog matches the MAC address of the
biosignalsplux device you want to update or upgrade. The MAC address of the device is
placed on its back. Click on the Check button.

The firmware update software will check the current version of the biosignalsplux and
display it in the dialog field.

8.2 Updating or Upgrading Firmware

If your device is running on an older firmware9 it is recommended to update or upgrade
the firmware for compatibility reasons with new features and to ensure that your device
works properly with the newest version of the OpenSignals (r)evolution software.

Open the Updater.exe you’ve downloaded and extracted. A new window will appear. Click
on the Find Devices button and wait for your MAC address to appear in the dialog bellows.
Check if the MAC address that is listed in the dialog matches the MAC address of the
biosignalsplux device you want to update or upgrade. The MAC address of the device is
placed on its back.

Click on Browse and browse the folder where the firmware .ENC file from the downloaded
package is placed and select the .ENC file. The firmware updater should now be similar
to the firmware updater in and click the Update button.

Figure 120: Firmware update tool is ready to update the firmware.

biosignalsplux will turn itself off two times during the firmware update or upgrade. Wait
for the biosignalsplux to turn itself on again after the first time (this may take some
seconds). The biosignalsplux firmware will now be installed on the biosignalsplux and the
device will automatically turn itself off for the second time after the installation is
finished. This time, check for the indications of the update software, and, if no different
indications are given, turn your device back on and confirm the dialog of the update
software by clicking on the OK button.

9
or if you need to upload another firmware to run with specific sensors (e.g. beta versions
of new sensors)

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When the firmware update/upgrade has finished, a dialog will pop up displaying the old
and the new version of the biosignalsplux firmware. new firmware was successfully
installed if the version of the new firmware matches your selected .ENC file and if it is
superior to the version of the displayed old firmware.

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9 Software & APIs

9.1 OpenSignals (r)evolution

OpenSignals is our easy-to-use, versatile, and scalable software for real-time biosignals
visualization, capable of direct interaction with all PLUX devices.

Core functionality includes sensor data acquisition from multiple channels and devices,
data visualization and recording, as well as loading of pre-recorded signals. OpenSignals
is also a Python-powered web-based software framework, targeted at rapid application
development; a bare bone code base is available on GitHub

In addition, OpenSignals has a suite of data analysis add-ons to create reports from the
recorded data and extract features directly from the signals without having to do any
coding. biosignalsplux Research and Professional lab kits already include some or all of
the add-ons upon purchase, although add-ons can also be purchased individually in our
web store.

You can download the newest version of OpenSignals (r)evolution here:

http://biosignalsplux.com/index.php/en/software/opensignals

9.2 OpenSignals Mobile

OpenSignals Mobile is a slimmed down version of OpenSignals (r)evolution specifically
designed to run on Android® mobile phones or tablets (iOS coming soon), while
preserving the ease-of-use and performance for real-time sensor data visualization and
recording.

OpenSignals Mobile is currently in development. However, if you are interested in being

one of the first few users to get your hands on a beta version of this app, visit our
OpenSignals website and fill in the form to subscribe for the early access beta version.

http://biosignalsplux.com/index.php/en/software/opensignals

9.3 APIs
It is possible to connect the devices to third party applications including your own custom
software. The needed toolkits to start your own biosignal software are delivered by PLUX
for free. All the functionalities and features of our high-end research devices can be
accessed by developers to integrate PLUX systems in custom and third-party applications.

Visit the official biosignalsplux API website to check the list of the available APIs:

http://biosignalsplux.com/index.php/en/development/apis

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10 Troubleshooting

10.1 Bluetooth
Most internal Bluetooth adapters are compatible with PLUX devices. However, if you’re
experiencing a great number of connection loss events, reconnection problems, missing
samples or similar problems, your internal Bluetooth module might not have enough
performance to deal with the data throughput of the device you are using. In this case,
reducing the sampling frequency or the sample resolution might solve this issue, if a
change of these parameters is possible.

For this reason, PLUX has identified Bluetooth dongles that have been tested and are
known to work with our devices. We recommend that you use one of these PLUX-tested
Bluetooth dongles, which are either already shipped with your device (e.g. in the case of
biosignalsplux kits) or can be purchased separately from our store. These dongles are
compatible to work with data rates up to 24 Mbps.

The solution is to exchange the internal Bluetooth Adapter for an external Bluetooth USB
dongle that uses the Windows native Bluetooth stack. For this, you’ll need to install the
Bluetooth dongle properly, as described in the following steps, which are explained in
more detail throughout the next pages for the supported operating systems:

Step 1: Removing already paired devices from your operating system

Step 2: Installing PLUX-proven Bluetooth dongle
Step 3: Changing Bluetooth Stack to Microsoft’s native Bluetooth stack

NOTE
If you’re already using PLUX devices with the old Bluetooth stack, please remove your
device(s) from your computer before changing the Bluetooth drivers/changing to
Microsoft Bluetooth Stack.

NOTE
We recommend going through all the following steps to set up your Bluetooth dongle
correctly, even if Windows may automatically install and set up your dongle.

NOTE
Installing the Bluetooth dongle and changing the Bluetooth stack is only required for
Windows computers only. Computers with a Mac OS X operating system do not need to
go through these steps and use the internal Bluetooth adapter instead.

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10.1.1 Windows 7 & Windows 8

Step 1: Removing already paired devices from your operating system

To remove Bluetooth devices, it is needed to access the Device Manager on your computer
and view the list of connected devices. The device list can be found following these steps:

Start Button > Control Panel > Hardware and Sound > View devices and printers

In the Device and Printers window, PLUX devices can be found in the Unspecified section.
Right-click on the device you want to remove and select Remove device (see Figure 121)

Figure 121: Windows 7 "Devices and Printers" window.

If the device is not listed under Unspecified anymore, it has been successfully removed
from your system.

Step 2: Installing PLUX-proven Bluetooth dongle

To install the PLUX-proven Bluetooth dongle, plug it in and install all the necessary
drivers for the Bluetooth dongle to work (an Internet connection is advisable). Windows
should display the window below.

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Figure 122: Wait for Windows to install your Bluetooth dongle.

Make sure you wait until your Bluetooth Dongle is installed. Windows will issue a
notification and you’ll be ready to configure the correct Bluetooth stack following the
next step.

Step 3: Changing Bluetooth Stack to Microsoft’s native Bluetooth stack

To configure the Bluetooth stack of the Bluetooth dongle, you have to connect your
Bluetooth dongle to your computer and access the Device Manager on your computer. The
device manager can be found following these steps:

Start Button > Control Panel > System and Maintenance > Device Manager

In the device manager, the Bluetooth dongle should be listed as Generic Bluetooth Adapter
(see Figure 123). If your machine already has an internal Bluetooth Adapter and you have
just plugged in the PLUX-proven Bluetooth Dongle, keep in mind that the last one will
be automatically disabled by Windows. This is why one of your Bluetooth adapters under
Bluetooth in the device manager shows a yellow warning icon ( ) stating: Code 10 -
This device cannot start.

Figure 123: Bluetooth dongle listed in the device manager.

If your Bluetooth dongle is the one disabled, you can manually disable your internal
Bluetooth adapter by right-clicking on it in the device manager and selecting Disable.
This action will enable your Bluetooth dongle by default.

If, after this action, your Bluetooth Dongle continues to display a yellow warning icon

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( ) , it means that it is not installed correctly and you should try to update the drivers
by right-clicking on it and selecting Update Driver Software... or fix the problem displayed
under Device Status in the device properties (right-click the Bluetooth device and select
Properties; see screenshot below).

Figure 124: First step to update the driver of the Bluetooth dongle.

Select Browse my computer for driver software from the new window.

Figure 125: Browse for driver software.

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Select Let me pick from a list of device drivers on my computer.

Figure 126: Pick driver from list of device drivers on the computer.

Select Generic Bluetooth Adapter and click on Next to install the Microsoft Bluetooth Stack.
Of several models of Generic Bluetooth Adapter are available you can select any of them.
It will not make any difference.

Figure 127: Select Generic Bluetooth Adapter

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Your device should now be configured properly and have no warning sign or error
message in the device manager. Rebuild the connection with your biosignalsplux device
(see Bluetooth Setup Windows 7) and test your device by recording signals with the
OpenSignals (r)evolution software. If, after all these steps, you keep experiencing the same
issues, contact our support

10.1.2 Windows 10

Step 1: Removing already paired devices from your operating system

To remove Bluetooth devices, it is needed to access the Bluetooth configuration of your
operating system. Click on the start button at the left lower corner in of your desktop to
open the start menu. Click on the gear symbol to open the settings panel of your
operating system as can be seen below.

Figure 128: Access to the settings panel in Windows 10.

In the settings panel, click on the Devices Bluetooth, printers, mouse field to open the
device configurations of your system.

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Figure 129: Windows 10 settings panel.

Click on your biosignalsplux and click on Remove device to remove this Bluetooth
connection from your computer and confirm the Are you sure you want to remove this
device? message that will be shown on your screen. Repeat this step with every
biosignalsplux if you’re using several devices.

Figure 130: Windows 10 Bluetooth devices

If, after this step, your device is not listed in the list of Bluetooth devices anymore, the
device has been successfully removed.

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Step 2: Installing PLUX-proven Bluetooth dongle

To install the PLUX-proven Bluetooth dongle, plug it into your computer. Windows will
install the Bluetooth dongle and display the window below.

Figure 131: Wait for Windows to install your Bluetooth dongle.

Make sure you wait until your Bluetooth Dongle is installed. Windows will issue a
notification and you’ll be ready to configure the correct Bluetooth stack following the
next step. Note, that this step might require a restart of your system. Make sure to close
all your open work and programs properly before restarting the system to prevent data
losses or other issues.

Step 3: Changing Bluetooth Stack to Microsoft’s native Bluetooth stack

To configure the Bluetooth stack of the Bluetooth dongle, you have to connect your
Bluetooth dongle to your computer and access the Device Manager on your computer.
Enter Device Manager into the search field of your taskbar and click on the listed device
manager.

In the device manager, the Bluetooth dongle should be listed as Generic Bluetooth Adapter
(see ). If your machine already has an internal Bluetooth Adapter and you have just
plugged in the PLUX-proven Bluetooth Dongle, keep in mind that the last one will be
automatically disabled by Windows. This is why one of your Bluetooth adapters under
Bluetooth in the device manager shows a yellow warning icon ( ) stating: Code 10 -
This device cannot start.

Figure 132: Bluetooth dongle listed in the device manager.

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If, after this action, your Bluetooth Dongle continues to display a yellow warning icon (
), it means that it is not installed correctly and you should try to update the drivers by
right-clicking on it and selecting Update Driver Software... or fix the problem displayed
under Device Status in the device properties (right-click the Bluetooth device and select
Properties; see screenshot below).

Figure 133: First step to update the driver of the Bluetooth dongle.

Select Browse my computer for driver software from the new window.

Figure 134: Browse for driver software.

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Select Let me pick from a list of device drivers on my computer.

Figure 135: Pick driver from list of device drivers on the computer.

Figure 136: Select Generic Bluetooth Adapter

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Your device should now be configured properly and have no warning sign or error
message in the device manager. Rebuild the connection with your biosignalsplux device
(see Bluetooth Setup Windows 10) and test your device by recording signals with the
OpenSignals (r)evolution software. If, after all these steps, you keep experiencing the same
issues, contact our support

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11 Safety & Maintenance

Please read the following safety instructions before using your biosignalsplux system to
prevent any damages or problems with the user, test persons and/or biosignalsplux
devices. Violations of these instructions can lead to inferior signal quality and/or damages
to the biosignalsplux system and user.

! The user should always keep the device and its accessories dry.

! The user must turn off the biosignalsplux device and contact Technical Support if
the system or accessories reach uncomfortable temperatures.

! The user should not use the biosignalsplux device in noisy environments
(environments with microwaves and other similar equipment). Doing so will lead
to noise increase in the acquired signals and Bluetooth connectivity issues.

! The user must not use the device near the fire or in potentially explosive
atmospheres, such as atmospheres with flammable gas.

! The user should only use the detection surfaces or other approved accessories
purchased from PLUX or by a PLUX agent.

! The user should inspect the sensors on a regular basis to ensure that they remain
in good working order.

! The user should stop using the biosignalsplux device if experience any kind of
discomfort or skin irritation.

! The user should not use the biosignalsplux device continuously for periods of time
above 60 minutes. Do not use the system on persons with allergies to silver.

! The user should dispose detection surfaces after using the biosignalsplux device.
Detection surfaces are single-user and disposable. Reusable electrodes should be
reused by the same user. Do not use reusable electrodes on several users.

! The user must not place the device in the microwave.

! The user must not insert objects into the holes of the device.

! The user should not open the biosignalsplux device or its accessories. The repair of
the same should be only done by properly authorized PLUX personnel.

! The user should make sure the cables do not obstruct the passage of people.

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! The user should use the sensor cables with extreme caution to avoid risk of
strangulation.

! The user should keep a safe distance between the biosignalsplux device and other
devices to ensure their proper functioning.

! The user should only send the device to repair to qualified PLUX personnel.

! The user should not immerse the sensors or the biosignalsplux device, nor clean
with liquid or abrasives.

! The user should handle the biosignalsplux device with caution and not expose the
device or accessories to high accelerations and vibrations.

! biosignalsplux devices should not be used in patients with implanted electronic

devices of any kind, including pace-makers, electronic infusion pumps, stimulators,
defibrillators or similar.

! Do not apply electrodes over damaged or irritated skin.

! Do not use your device while charging its internal battery.

11.1 Maintenance Recommendations

11.1.1 Transportation and Storage

Please follow these recommendations to ensure safe transportation and storage of your
biosignalsplux equipment and sensors to prevent any damaging of your system.

• The biosignalsplux equipment and sensors should be stored in the original box in
a dry place when those are not being used.
o Relative humidity: up to 95% with no condensation
o Ambient temperature: 10°C to 30°C
o Atmospheric pressure between 500hPa and 1060hPa

• Whenever the equipment needs to be transported, it should be placed in the

original box, since this was designed and tested to ensure the equipment and
accessories are securely stored.

• Take care while handling the bac and avoid dropping it, since the device is not
shock-proof and should not be placed under stress or sudden acceleration.

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11.1.2 Cleaning
Please follow these cleaning instructions to prevent any damage of the system or the
user because of conducting cleaning methods that may cause any damage.

• The biosignalsplux and sensors should be visually checked before each use and
cleaning process to ensure that no mechanical damage occurred.

• The biosignalsplux equipment and sensors (including the cables) should be

cleaned with a slightly damp cloth or suitable absorbent paper, ensuring no liquid
enters the equipment of sensors. Do not use detergent or any type of cleaning
liquid as these may damage your equipment and/or sensor.

• Do not clean or re-use detection surfaces (electrodes). They are only suitable for
single use, and should be disposed of after usage except indicated otherwise.

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12 Regulatory & Legal Information

12.1 Disclaimer
biosignalsplux products are intended for use in life science education and research
applications only; biosignalsplux products are not medical devices, nor medical software
solutions, nor are they intended for medical diagnosis, cure, mitigation, treatment or
prevention of disease and is provided to you “as is”.

12.2 Guarantee of Quality & Warranty

The biosignalsplux acquisition system has a two years quality guarantee from the date of
purchase. biosignalsplux sensors have three months quality guarantee from the date of
purchase. PLUX guarantees that the system, sensors and accessories will be free from
material or manufacturing defects for the mentioned time periods following date of
purchase.

If PLUX receives notification of any such defects within the guarantee period, it will repair
or substitute with the same unit\model, any products with proven defects at no cost to
the client. During the repair period PLUX promises to provide a temporary replacement
under the same specification. Repairs will be carried out at PLUX’s premises after the
equipment has been received.

12.2.1 Warranty Voidance

Usage of the device that is not in accordance with the handling instructions indicated in
the manual, or use with accessories other than those manufactured by PLUX will
invalidate the warranty of your devices.

Be careful when connecting your biosignalsplux devices, sensors and/or accessories to any
third party device including the usage of the 3rd party connection components that are
available for biosignalsplux systems as the usage of these components will void the
electrical warranty of your biosignalsplux device and sensors and, if not indicated
otherwise, the warranty of the 3rd party system you’re connecting to the device. Check the
electrical specifications of both systems you want to connect to prevent any damage of
the user(s) or the systems.

In the case of warranty voidance, the same applies that we expressly disclaim any liability
whatsoever for any direct, indirect, consequential, incidental or special damages,
including, without limitation, lost revenues, lost profits, losses resulting from business

154 of 159
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interruption or loss of data, regardless of the form of action or legal theory under which
the liability may be asserted, even if advised of the possibility of such damages.

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12.3 Contact & Support

Contact us if you’re experiencing any problems that cannot be solved with the
information given in the biosignalsplux or OpenSignals (r)evolution manual. We’ll get back
to you as soon as possible to find the best solution for your problem.

Please send us an e-mail with precise information about the error occurrence, device
configuration, and, if possible, screenshots of the problem to support@plux.info.

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13 Datasheets

The information displayed in this manual is taken from the official biosignalsplux
datasheets. Please read the datasheets of your hub, sensors, and/or accessories before
using them. The datasheets can be accessed via the links below.

biosignalsplux Hub
http://biosignalsplux.com/datasheets/biosignalsplux_hub_Spec_Sheet.pdf

Accelerometer (ACC)
http://biosignalsplux.com/datasheets/ACC_Sensor_Datasheet.pdf

Blood Volume Pulse (BVP)

http://biosignalsplux.com/datasheets/BVP_Sensor_Datasheet.pdf

Breakout Cable
http://biosignalsplux.com/datasheets/IO_Breakout_Cable.pdf

Electrocardiography (ECG)
http://biosignalsplux.com/datasheets/ECG_Sensor_Datasheet.pdf

Electrodermal Activity (EDA)

http://biosignalsplux.com/datasheets/EDA_Sensor_Datasheet.pdf

Electroencephalography (EEG)
http://biosignalsplux.com/datasheets/EEG_Sensor_Datasheet.pdf

Electromyography (EMG)
http://biosignalsplux.com/datasheets/EMG_Sensor_Datasheet.pdf

Goniometer (GON)
http://biosignalsplux.com/datasheets/GON_Sensor_Datasheet.pdf

Handheld Switch
http://biosignalsplux.com/datasheets/BTN_Sensor_Datasheet.pdf

LED
http://biosignalsplux.com/datasheets/LED_Actuator_Datasheet.pdf

Light (LUX)
http://biosignalsplux.com/datasheets/LUX_Sensor_Datasheet.pdf

Load Cell
http://biosignalsplux.com/datasheets/Load_Cell_Datasheet.pdf

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Respiration (RIP)
http://biosignalsplux.com/datasheets/RIP_Sensor_Datasheet.pdf

Respiration (PZT)
http://biosignalsplux.com/datasheets/PZT_Sensor_Datasheet.pdf

Synchronization Kit (SYNC) (incl. Digital Synchronization Cable & Multi Sync Splitter)
http://biosignalsplux.com/datasheets/SYNC_Accessory_Datasheet.pdf

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Biosignalsplux User Manual

Uploaded by

Biosignalsplux User Manual

Uploaded by

biosignal acquisition tool-kit for advanced research applications

Last update: 01.02.2018

PLUX Wireless Biosignals S.A.

3.2.10 SpO2 Reader (OSL) ........................................................................................................76

10.1 Bluetooth ..................................................................................................................... 140

1 Your biosignalsplux Kit

Figure 1: biosignalsplux kit.

1.1 biosignalsplux Explorer

Figure 2: biosignalsplux Explorer Kit

This kit contains:

• 1 wireless 4-channel biosignalsplux HUB

Optional components, features, and add-ons1:

• 1 digital I/O port

1.2 biosignalsplux Researcher

Figure 3: biosignalsplux Researcher Kit

This kit contains:

• 1 wireless 8-channel biosignalsplux HUB

• OpenSignals (r)evolution Real-Time Event Emulation Add-On

Optional components, features, and add-ons2:

• 8GB internal memory

1.3 biosignalsplux Professional

Figure 4: biosignalsplux Professional Kit.

This kit contains:

• 1 wireless 8-channel biosignalsplux HUB

• OpenSignals (r)evolution signal acquisition and visualization software (download

Optional components and features3:

• Advanced sensors (see 3.2 Advanced Sensors)

1.4 biosignalsplux Force

Figure 5: biosignals Force

This kit contains:

• 1 wireless 4-channel biosignalsplux HUB with 8GB internal memory

• OpenSignals (r)evolution Center of Mass Analysis Add-on

Optional components, features, and add-ons:

• 1 digital I/O port

1.5 ergoplux Explorer

This kit contains:

• 1x Wireless 4-channel hub (see specs below)

1.6 ergoplux Explorer

This kit contains:

• 1x Wireless 8-channel hub (see specs below)

Figure 6: biosignalsplux hub

biosignalsplux is compatible with all available biosignalsplux professional sensors (3.1

Intended Use for Research Applications

2.1 4-Channel Hub Specifications

Figure 7: 4-channel biosignalsplux hub.

2.2 8-Channel Hub Specifications

Figure 8: 8-channel biosignalsplux hub.

2.3 Ports and Peripheries

Figure 9: biosignalsplux ports and the button.

2.3.2 Analog Inputs

2.3.4 Digital Port

2.3.5 Charging Port

2.3.6 Button Behavior

2.4 General Device Functionality

2.4.1 Turning on the Device

Figure 10: biosignalsplux center button.

2.4.2 Connecting the Sensors

Figure 11: biosignalsplux side view.

2.4.3 Disconnecting the Sensors

• don’t twist the sensor while disconnecting

If you’re experiencing a great number of connection loss events, reconnection problems,

Operating frequency range 2400 – 2483.5 MHz ISM Band

3.1 Professional Sensors

3.1.1 Electromyography (EMG)

Figure 13: EMG sensor.

Electrode cables & sleeves

How to connect your sensor to your biosignalsplux

This EMG sensor has no built-in reference electrode. We recommend connecting a

How to configure your sensor in OpenSignals (r)evolution

An example configuration is displayed in Figure 14 (here: EMG connected to channel 1).

Figure 14: EMG configuration in OpenSignals.

Figure 15: biosignalsplux EMG sensor sample.

OpenSignals (r)evolution Add-On: Electromyography Analysis

3.1.2 Electrocardiography (ECG)

Figure 16: ECG sensor.

Electrode cables & sleeves