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P530/3
BIOLOGY
PRACTICAL
JULY, 2019
3¼ Hours
                            Uganda Advanced Certificate of Education
                         RESOURCEFUL MOCK EXAMINATION 2019
                                     BIOLOGY PRACTICAL
                                              Paper 3
                                          Time: 3¼ Hours
INSTRUCTION
        This paper consists of three questions.
        Answer ALL questions.
        Write your answers in the spaces provided. Additional sheets of paper must not be
         inserted in this booklet.
        You are not allowed to start working within the first 15 minutes. You are advised to use
         this time to read through the paper and ensure that you have all the apparatus, chemicals
         and specimens you may require.
                                        For Examiner’s use ONLY
                       Question      Mark               Examiner’s initial
                       1
                       2
                       3
                       TOTAL
1. You are provided with specimen K which is freshly killed.
   a. Relate the features of the following parts to significance for the organism’s survival
       in its habitat.
       i.      Dorsal skin         (2 marks)
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       ii.     Foot of hind limb         (2 marks)
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   b. Place the specimen ventral side up with head towards you. Using forceps, open the
       mouth fully to expose and examine structures within the buccal cavity responsible
       for feeding. Draw and label.                    (9 marks)
c. Dissect the specimen to expose vessels responsible for
   i.      taking blood to the left and upper trunk regions from the heart
   ii.     carrying blood from the thoracic region, urinary organs and structures
           responsible for chemical digestion back to the heart. With the heart turned up,
           draw and label the vessels.      (27 marks)
2. You are provided with plant tissues B1 and B2 which have been treated differently and
     solutions Z and X.
a. Cut tissues B1 and B2 into four equal cube pieces. Obtain the same size of tissues from
     lung and thigh muscle of specimen K in question 1.
     Label test tubes 1, 2, 3, 4, 5, 6, and 7. Add contents to each of the test tubes as shown in
     table 1 and in each case record your comparative observations and deductions. (14 marks)
 Test tube Contents                      Observation                            Deduction
 1            2cm3 of Z and cube of
              lung.
 2            2cm3 of Z and cube of
              muscle.
 3            2cm3 of Z and one
              cube of B1
 4            2cm3 of Z and one
              cube of B2
 5            2cm3 of Z and one
              crushed cube of B1
b. Explain your results for the following test tubes
   i.      Test tube 1 and 2         (7 marks)
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   ii.     Test tubes 3, 4 and 5     (5 marks)
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c. Suggest two conclusions that can be drawn from your results and give a reason.
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d. In test tubes 6 and 7 add into each 5cm3 of solution X. Dip two of the remaining
   cubes of B1 into test tube 6 and two cubes of B2 into test tube 7. Leave to stand for 30
   minutes.
   i.      Measure the final volume in test tubes 6 and 7. Explain the changes in
           volume.             (6 marks)
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       ii.    Examine and state the physical state of tissue from test tube 6. (3 marks)
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3. You are provided with specimen P, Q, R and S which are plant organs.
   a. Describe the floret arrangement in
       i.     Specimen Q                (2 marks)
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       ii.    Specimen R                (2 marks)
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   b. Describe the structure of comparative features of bracts in floret of P.   (3 marks)
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   c. How are the following features adapted for pollination?
       i.     Stamen of floret in P             (2 marks)
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   ii.    Observable gynoecium feature of S.             (2 marks)
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d. Carefully obtain one inner floret from specimen Q observe using a hand lens, draw
   and label.               (6 marks)
e. Construct a dichotomous key for the identification of specimen P, Q, R and S using
   only essential reproductive structural features.      (3 marks)
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                                      --( THE END )--
S.6 BIO RESOURCEFUL 2 CONFIDENTIAL
Requirements
      Toad labelled K
      1cm3 of fresh Irish potato cube labelled B1
      1cm3 of slightly boiled Irish potato and cooled labelled B2
      15cm3 of 5% hydrogen peroxide labelled Z
      15cm3 of 1M sucrose solution labelled X
      Male maize inflorescence branch with hanging anthers labelled P
      Biden pilosa inflorescence labelled Q
      Bougainvillea inflorescence labelled R
      Panicum maximum inflorescence branch with floret stigma exposed
       labelled S
      7 test tubes
      2 slides
      Labels
      10cm3 measuring cylinder
      Razor blade
      Small mortar and pestle