[go: up one dir, main page]
Scribd
Upload
755 views2 pages

Assay of Phenobarbitone

The document describes an experiment to determine the percentage purity of phenobarbitone through a non-aqueous titration process. Phenobarbitone is dissolved in acetic acid and titrated with perchloric acid until the solution changes color, indicating the endpoint of the titration. The volume of perchloric acid used is then used to calculate the percentage purity based on the weight and normality values provided.

Uploaded by

Aditya Thakkar
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
755 views2 pages

Assay of Phenobarbitone

The document describes an experiment to determine the percentage purity of phenobarbitone through a non-aqueous titration process. Phenobarbitone is dissolved in acetic acid and titrated with perchloric acid until the solution changes color, indicating the endpoint of the titration. The volume of perchloric acid used is then used to calculate the percentage purity based on the weight and normality values provided.

Uploaded by

Aditya Thakkar
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd
You are on page 1/ 2

Not-Aque0iUsTitrations

O0 95

Experiment No. 3

Assay of Phenobarbitone

AIM

Tofind out the


percentage purity off Phenobarbitone.
APPARATUS REQUIREMENTS
ml), Volumetric flask, Funnel, Measuring cylinder.
Burette, Pipette (20
REAGENTS
perchloric acid, crystal violet,
phenobarbitone,glacial acetic acid, mercuricacetate, 0.1N
1-napthobenzoin.
PRINCIPLE
titration. Phenobarbitone get hydrated with
Phenobarbitone is assayed by non-aqueous
group.
acetic acid in imino
CH,CH,
CH,CHs
CH,COOH + HN
HN

N
H acid act as a base. Hence it react with
acetic
presence of stronger perchloricacid,
In the acetonium ion.
perchloric acid to form -’CH,COOH,*+CI0,
HCIO, + CH,COOH proton to conjugate base i.e., acetate ion.
donates its
Acetonium ion readily
2CH,COOH of base with
CH,COOH,' +CH,CO’ during titration. This replaces chloride
is added
Mercuric acetate solution
much stronger base than halide ion.
dCetate.This acetate ion is
+2CI-’ HgCl,
(CHCOO),Hg standardised +2CH,COOhydrogenphthalate.
by potassium
Perchloric acid is and
30 ml of glacial acetic acidcrystal
PROCEDURE it with acid using blank
Phenobarbitoneand dissolve with 0.1N perchloricPerform the
veigh 500mg of acetate. Titratethis solution changes to green.
add 10 ml offmercuric end point the colour
Violet as an indicator. At the
determination.
96 O0
Pharmaceutical Analytical Chemistry
gn of Phenobarbitone
Each ml of 0.1 N Perchloric acid = 0.02542
OBSERVATION
Titration of Phenobarbitone with 0.1N Perchloric acid
S.No. Volume of Phenobarbitone Burette reading Volume of 0.1 Perchloric
(ml) Initial Final acid (ml)

2.
3.

CALCULATION
T.Vx Normality of 0.1NPerchloricacidxI.P.factorx 100
Percentage purity = Weight takenx Exp.Normality
RESULT
The percentage purity of the given Phenobarbitone

You might also like