Activity No.
4
NAME: PACALDO, ANNISSA J. DATE PERFORMED: ________
GROUP NO: _________________________ DATE SUBMITTED: ________
SECTION: ST12P3 RATING: ________________
Drosophila Life Cycle
Objective:
• To describe the different stages of animal
development.
Introduction:
Some of the most important information discovered about fruit
flies involves chromosomes. Chromosomes contain DNA in cells
that gives instructions for how organisms function and
develop. Humans have 23 pairs of chromosomes. Fruit flies have
only 4. One of the four pairs of chromosomes is called the sex
chromosomes. How the sex chromosomes are paired determines
whether the fly is male or female. Male fruit flies, and male
humans, have an X and Y chromosome. Female fruit flies, and
female humans, have two X chromosomes. In the early 1900s,
fruit fly geneticist Thomas Hunt Morgan used his extensive
data on multiple fruit fly generations to determine how
chromosomes were related to genes in all organisms.
Materials:
• Overripe banana
• One quart jar
• Large rubber band
• Magnifying glass or dissecting microscope
Procedures:
1. Peel the banana.
2. Add a small amount of the food medium into a bottle. (The
bottle should only be approximately 1/10 full.) Use the
same type of bottle as that used to catch fruit flies so
that the mouth openings will be the same. Be sure to
flatten the medium at the bottom of the bottle so that
the medium will not fall off if the bottle is placed
upside down.
3. Transfer the fruit flies from the catch bottle to the
culture bottle. Do this by placing the culture bottle on
top of the catch bottle with their mouths touching each
other. Remove the paper cover of the catch bottle and let
the flies move to the medium bottle. When there are at
least 10 flies in the culture bottle, immediately cover
the bottle with paper secured with rubber bands.
4. Store the culture bottles in a cool, dry place in the
class room or laboratory. Place the bottles on a shallow
basin with water to prevent ants from going into the
medium.
Observation:
A. Take note of the date when the flies were cultured.
This is indicative of the approximate time the eggs will
be laid.
B. Record the date when the larvae first emerged. These
will appear as small maggots crawling on the medium or at
the inner side of the bottle. Draw or take pictures of
the larvae.
C. Record the date when the pupae first emerged. These
will appear as small cocoons sticking at the inner side
of the bottle. Draw or take pictures of the pupae.
D. Record the date when the adult flies first emerged.
Draw or take pictures of the adult flies.
E. Draw the life cycle of the fruit fly. Include the
approximate number of days it takes for each stage to
emerge based on observations.
Table 2. Observing the Life cycle of the fruit flies
Date when the Date when
Date when Date when adult
larvae 1st pupae 1st
flies were flies emerged
emerged emerged
cultured
April 1, 2024 Until now, ✓ April 16,
Larvae have there is no 2024
not developed development
of larvae. 16 days have
So, there is passed and there
no pupae. are no visible
adult flies.
Drawing:
Draw the life cycle of a fruit fly. Include the
approximate number of days it takes for each stage to
emerge based on observations. (On the next page provided)
Conclusion:
According to my research, when flies are caught, they lay eggs
in about 5 to 10 days. In my container with a ripe banana and
flies, I saw eggs after 8 days. These eggs then turn into
larvae after about 3 to 10 days. But in my container, the
larvae didn't appear even after 16 days had passed.
Furthermore, after larvae, the pupae supposed to start showing
up on the ground between 10 to 25 days later. Once a pupa has
formed, it turns into an adult fly in about 8 to 40 days. In
conclusion, the life cycle of a fruit fly takes a long time.
According to my findings, fruit flies usually live for about
40 to 50 days. Therefore, it’s the reason why I still haven't
seen any adult flies in my container yet, even a larva, as
I've only been observing for 16 days, or there are some
factors that causes the life cycle of a fruit fly in my
container doesn’t emerge every part of it.
The Drosophila Life Cycle
DOCUMENTATION
✓ Making the container and placing it near
the trash bin to catch fruit fly.
✓ Fruit fly have been cultured
✓ After 16 days |STILL NO LARVAE
