ZOO 4232 HUMAN PARASITOLOGY

FALL SEMESTER, 2023

Hybrid
In-person in TurL005 and Distance
MWF 8:30 – 9:20 am

INSTRUCTOR: Dr. PETER KIMA, Micro. Cell Science Building, Room 1005,
392-0384. Email: pkima@ufl.edu

Credits: 3

Textbook: Human Parasitology. Burton J. Bogitsh and Thomas C. Cheng.

Academic Press. San Diego CA., 4th Edition. ISBN. 978-0-12-
415915-0. Other recommended texts: Cell and Molecular Biology
by Gerald Karp. John Wiley & Sons, Inc.; Immunobiology, The
Immune System in Health and Disease. Charles Janeway and Paul
Travers. Garland Publishing Inc, New York NY.
 Hybrid course
Lectures: Most lectures will be delivered in in-person class
sessions. The lectures will be recorded and made accessible to all
students. Some lectures will be pre-recorded. Here is the zoom link
to access lectures synchronously.

You are invited to a Zoom meeting.

When: Aug 23, 2023 08:30 AM Eastern Time (US and Canada)

Register in advance for this meeting:

https://ufl.zoom.us/meeting/register/tJAvf-
CgqT4tG9Vb5MhRyyLjFjdqvT_DSq3n

After registering, you will receive a confirmation email containing

information about joining the meeting.

Meeting ID 942 8987 5711

Passcode 994211
 • Office hours – Tuesdays 3 -5pm
Peter Kima is inviting you to a scheduled Zoom meeting.

Topic: ZOO4232 office hours

https://ufl.zoom.us/j/96816372051?pwd=MTJMMndXOHgxTXRvOWh6QTN3aHZlZz09

Meeting ID 968 1637 2051

Passcode 616715
Course Website The course website on canvas contains pertinent information on the
course. The website is subdivided into 3 pages:

Lectures: Most lectures will be delivered in in-person class

sessions. The lectures will be recorded and made accessible to all
students. Some lectures will be pre-recorded. Lectures can be
accessed synchronously.

All recorded lectures will be placed in a library of recorded lectures

with links in the Lectures module. Powerpoint presentations for
each lecture (saved as pdf) will be posted in the ‘Lectures’ module.
Exam questions will be extracted from material in lectures.

Lecture-associated readings: The Lecture-associated module will

contain insightful case reports of infections as well as short
discussions of useful topics. The material in the Lecture-associated
module is intended to reinforce the course objectives and to
enhance your overall understanding of parasitism and host-
pathogen interactions.

Assigned readings. Assigned readings module will contain recent

review articles or primary source articles. You will be assigned case
reports, primary papers and review articles to compliment the
concepts discussed in lecture. You will be expected to gain a
detailed understanding of the assigned readings. The assigned
readings will have some annotations to help enhance your
understanding of the articles. Teaching Assistant(s) will be
available to discuss the readings with you. A quiz with at least 10
questions will be extracted from the assigned readings. You
will have up to 10 days to complete the assigned readings and
quiz.
Class Attendance: Class attendance is discretional (this is a hybrid course).
Gainesville students are encouraged to attend in person lectures. However, all material
will be recorded and made available to everyone.

Exams and quizzes: There will be 4 proctored class exams.

Gainesville students are encouraged to take the exam in person at the scheduled
class time. Alternatively, students can take the exam proctored through ProctorU.
The exams will be proctored by ProctorU. Each student will schedule a time to take
the exam on the exam dates in the syllabus. Each exam will be available for 2 days.
Each student is expected to have sufficient battery life to last for the entire 50 min
exam. Please note the exam make-up policy below.

Quizzes:

There will be 2 types of quizzes.

Type 1 quizzes will be extracted from lectures. Following lecture blocks, quizzes
extracted from those lectures will be made available. You will have 7 to 10 days to
complete each quiz. Students are expected to work independently.

Type 2 quizzes will be extracted from the assigned readings. Availability of the
assigned readings will be communicated by email to all students. Type 2 quizzes will be
‘open book’. You will have 2 tries for each quiz. Only your higher score will be recorded.
Although the quizzes are ‘open book’, students are expected to take them
independently.
Grading: Students in this class will be graded based on Exams and Quizzes.

There will be 4 exams each worth 15% of the final Grade (Exams
will be worth 60% of your grade). Exams will be proctored by
ProctorU or in class by the Instructor

No exam make-ups: Exams are available over a 2-

day period and can be taken from any where that has
accessible WIFI. Make notice of exam dates in the
syllabus and make appropriate arrangements!

Quizzes will add up to 40% of your grade

-Type 1 quizzes will be worth 20% of your final grade

-Type 2 quizzes will be worth 20% of your final grade.

You will have 7 or 10 days to complete each quiz. Please make

every effort to complete your quizzes and exams at scheduled
times.

Letter grades including minus grades will be determined solely from

scores on exams and quizzes. The point cut offs for letter grade
assignment will be posted after the second exam.
 Class Exam Dates

Exam 1 September 21-22 (Thurs/Fri)

Exam 2 October 19/20 (Thus/Fri)

Exam 3 November 13-14 (Mon/Tues)

Exam 4 December 14 (Tues @ 12:30 PM - 2:30 PM (Tur

L005)
 ZOO 4232 Human Parasitology Lecture Schedule
Fall Semester, 2023

Date Topic Instructor

August 23 (Wed) Introduction to Course P. Kima

25 (Fri) Primer/Review-The Eukaryotic Cell P. Kima

28 (Mon) Primer/Review- The Host Immune Response P. Kima

30 (Wed) Primer P. Kima

September 1 (Fri) Primer P. Kima

4 (Mon) No Class - Labor day

6 (Wed) Protozoa: General Features/Visceral Protozoa P. Kima

8 (Fri) Visceral Protozoa (Amoeba and Ciliates) P. Kima

11 (Mon) Visceral Protozoa (Amoeba and Ciliates) P. Kima

13 (Wed) Visceral Protozoa (Flagellates) P. Kima

15 (Fri) Visceral Protozoa (Flagellates) P. Kima

18 (Mon) Visceral Protozoa (Flagellates) P. Kima

20 (Wed) Visceral Protozoa (Flagellates) P. Kima

21-22 (Thu/Fri) Exam 1 (Eukaryotic cell/Immune Response/Visceral Protozoa)

December 01 (Fri) Intestinal nematodes

03 (Mon) Blood and Tissue Nematodes P. Kima

06 (Wed) Blood and Tissue Nematodes P. Kima

Tuesday 12/14/2023 @ 12:30 PM - 2:30 PM (in class). Accessible via ProctorU all day,
12/14/2023. (Exam 4 - Lectures on Nematodes)
 IMPORTANT NOTES

Academic Honesty: As a result of completing the registration form at the University of

Florida, every student has signed the following statements: I understand that the
University of Florida expects its students to be honest in all academic work. I agree to
adhere to this commitment to academic honesty and understand that my failure to
comply with this commitment may result in disciplinary action up to and including
expulsion from the University.

University of Florida Counseling Services: On-campus resources are available for

students having personal problems or lacking clear career and academic goals that
interfere with their academic performance. These resources include:

1 University Counseling Center: 301 Peabody Hall, 392-1575, personal and

career counseling.

2 Student Mental health: Student Health care Center, 392-1171

3 Sexual Assault Recovery Services (SARS): Students Health Care Center,

392-1161, sexual counseling.

4. Career Resource Center: Reitz Union, 392-1601, career development

assistance and counseling.

5. Consult resources for students on your campus (Distance)

Scope: This course will present information concerning parasite life cycles,
biology of host-parasite relationships, vectors of parasites,
epidemiology, methods of diagnosis, prevention and control of
parasitic infections of public health importance.

Objectives: The student is expected to

• obtain a detailed understanding of the life cycle patterns of major parasites of

importance to humans
• Appreciate the cell biological aspects of the biology of these parasites and
host/parasite interactions
• Obtain some understanding of the host immune response to these infections as
presented in lecture, the assigned textbook and suggested reviews
• Learn the epidemiology of the diseases associated with these parasites
• Become familiar with Vectors of parasites; vector-parasite relationships
• Understand strategies to control or limit their effects on humans
• Gain a basic appreciation of significant unresolved questions relating to some of
these parasites and their relationships with humans.
 Case Report
• A 14 year old boy presented in a hospital in St John’s County
complaining of headaches, vomiting, fever and malaise. His past
medical history was unremarkable.
• It is known that he had been swimming for most of the summer in a
pond dug behind his mother’s house. No one else who had been
swimming in this pond had similar complaints. Gram stain and culture
of CSF fluid was most likely negative.
• The boy died a week after onset of symptoms.

• Differential Diagnosis: Meningococcal meningitis, Pneumonococcal

meningitis, viral encephalitis etc.
• Diagnosis: Primary amebic meningoencephalitis caused by Naegleria
fowleri. Viewed in wet mount of CSF. This organism is found in warm
fresh water lakes and ponds in Florida and southern U.S.

• Naegleria is a free living amoeba that sometimes infects humans.

 Teen dies of rare disease caused
by "brain-eating" amoeba
• August 17, 2011 9:33 AM PrintText
• By David W Freeman Topics News
,Kids and family ,Fitness .28
Comments
• (CBS/AP) Amoeba are being blamed
for the brain infection that killed a
Florida teen.
• Courtney Nash, 16, fell ill and died of
the rare disease after swimming in a
river that harbored the infectious
microbes, the CDC confirmed on
Monday. The girl's family says she
died
• amoeba
• 23:23 PM, Aug 13, 2015A deadly brain infection caused by an amoeba killed an
Oklahoma resident after a recent lake swim, state health officials said.
• Officials: Brain-eating amoeba did not cause teen's death
• 21:17 PM, Aug 11, 2015Bacterial meningitis killed a 14-year-old boy in Minnesota
last month, not a brain-eating amoeba as some officials initially suspected. CNN
affiliate WCCO reports.
• Brain-eating amoeba didn't cause teen's death
• 23:43 PM, Aug 10, 2015Bacterial meningitis killed a 14-year-old boy in Minnesota
last month, not a brain-eating amoeba as some officials initially suspected.
• Brain eating amoeba
• 02:36 AM, Jul 14, 2015Dr. Sanjay Gupta explains a brain eating amoeba found in
freshwater lakes,
 From CNN – August 19, 2008

Lawsuit says eatery to blame for 9-foot tapeworm

A man who contends he got a 9-foot tapeworm after eating

undercooked fish has sued a Chicago restaurant.

In the lawsuit filed Monday, Anthony Franz said he ordered

salmon salad for lunch from Shaw's Crab House in 2006 and fell
violently ill. He later passed the giant parasite, which a
pathologist determined came from undercooked fish, such as
salmon.
 Case Report
• This is the case of an 18 year old female visiting the United States
from India who presented to St. Francis Hospital in CT after having
had a seizure episode. The patient did not have a history of previous
seizures. She did not complain of any headaches, abdominal pain or
diarrhea. She had not lost weight.
• She lived in India with her parents and was attending College at the
time. She was sexually active and acknowledged condom use. She
denied any history of injectable drug use. She indicated that she was
always healthy and had never been hospitalized.
• She appeared well upon physical exam. Was afebrile. The examination
(including the neurological exam) was essentially unremarkable. Lab
data did not reveal any significant finding. Her Chest X-ray was
normal. However, CT scan of the head revealed multiple small circular
densities. What was the cause of her seizures?
Representative Laboratory
Findings
• Differential Diagnosis: In individuals who live in the U.S., are
young and sexually active, toxoplasmosis (as a possible complication
of HIV infection) will be considered. It is also likely that the patient
has a malignancy (lymphoma, gliobastoma etc.)
• Diagnosis: In individuals from Mexico, Central and South America,
South East Asia, such a presentation is most likely due to the presence
of larval cysts of the cestode Taenia solium (pork tapeworm). The
infection is termed neurocysticercosis. In the regions mentioned above,
T. solium is the most common parasitic infection of the CNS.
• The cysts act as space occupying lesions. When cysts degenerate, they
evoke an inflammatory response that can result in seizures. The
circular densities in the CT scan are calcified scolex (head) of the
worm
• T. solium is most often acquired by ingestion of (infected)
undercooked pork and parasite eggs.
ID boards exam question
this smear was obtained from a heart
transplant recipient; what is your
diagnosis Dr.?
 Case Report
• A 39 year old man presents to the emergency department with a 4 day
history of worsening dyspnea (shortness of breath), fevers, shaking
chills and a 2 week old nonproductive cough. He complains of recent
weight loss. He denies abdominal pain, diarrhea and recent travel. The
patient is HIV positive, and his last CD4 T cell count was 350/ul (2
years prior) [Normal ~1000-1200/ul].
• Significant laboratory findings include bilateral interstitial and alveolar
opacities observed in chest radiograph.

• Diagnosis: The patient has pneumonia. The most common cause of

pneumonia in patients with HIV whose CD4 T cell count is below
200/ul is Pneumocystis carinii (P. carinii pneumonia or PCP).
• Taxonomic classification of P. carinii is still being debated. Is it a
fungus or a protozoan parasite? The organism is difficult to culture and
little is known of its biology.